Nghiên cứu mức độ biểu hiện của micro RNA 29a, micro RNA 146a và micro RNA 147b tự do huyết tương ở bệnh nhân đái tháo đường týp 2 TT TIENG ANH

MINISTRY OF EDUCATION MINISTRY OF DEFENSE AND TRAINING VIETNAM MILITARY MEDICAL UNIVERSITY PHAN THE DUNG THE EXPRESSION OF CIRCULATING MICRO-RNA 29A, MICRO-RNA 146A, AND MICRO-RNA 147B LEVELS IN PLASMA OF PATIENTS WITH TYPE DIABETES MELLITUS Specialization: Internal medicine Code: 97.20.107 SUMMARY OF DOCTORAL DISSERTATION IN MEDICINE HA NOI - 2021 The study was completed at: VIETNAM MILITARY MEDICAL UNIVERSITY Supervisors: Assoc Prof Dr Doan Van De Prof Dr Nguyen Linh Toan Reviewer 1: Dr Nguyen Van Do Reviewer 2: Prof Dr Nong Van Hai Reviewer 3: Assoc Prof Dr Nguyen Minh Nui The disseratation will be defended in front of the Board of Dissertation Assessment, Vietnam Military Medical University at on month year The dissertation could be found in: 1- Vietnam National Library 2- Library of Vietnam Military Medical University THE PUBLISHED ARTICLES RELATED TO THE DISSERTATION Phan The Dung, Doan Van De, Nguyen Linh Toan, (2020) The beta cell function disorder and insulin resistance status in patients with type diabetes mellitus Vietnam Medical Journal, 494(1):17-20 Phan The Dung, Doan Van De, Nguyen Linh Toan, (2020) Plasma micro-RNA 29a, micro-RNA 146a and micro-RNA 147b expression level in patients with type diabetes melitus Jounal of military pharmaco-medicine, 45(7):83-88 LIST OF ABBREVIATION IN THE THESIS No Abbreviation Full text β Beta BMI Body Mass Index cDNA Complementary DNA Ct Threshold cycle HOMA-β Homeostasis Model Assessment Beta cell function HOMA-IR Homeostasis Model Assessment Insulin Resistance HOMA-S Homeostasis Model Assessment insulin sensitivity MiR Micro-RNA mRNA Messenger RNA 10 RNA Ribonucleic acid 11 RT-PCR 12 T2DM Type diabetes militus INTRODUCTION Diabetes mellitus is one of the non-communicable diseases with a rapid growth rate The pathogenesis of type diabetes mellitus (T2DM) is insulin resistance, impaired beta cell function Micro-RNAs (miRs) are RNAs with a molecular size of 19-24 nucleotide, non-protein-coding RNAs that regulate gene expression at the post-transcriptional level by inhibiting transcription or destroying messenger RNAs (mRNA) Extracellular miRs have been reported to act as signaling molecules to mediate cell-to-cell communication Recent studies suggest that miRs are directly and indirectly involved in the pathogenesis of T2DM and a potential therapeutic approach for diabetes Some recent studies show that miR-29a, miR-146a participate in the pathogenesis of diabetes but these have not been fully studied yet Meanwhile, miR-147b has been shown to be involved in programmed cell death and little information is available about miR-147b studies in patients with T2DM In Vietnam, there are still no studies mentioning the role of micro-RNAs, especially miR-29a, miR-146a and miR-147b in plasma in T2DM Therefore, we have conducted a research with subject “The expression of circulating micro-RNA 29a, microRNA 146a and micro-RNA 147b levels in plasma of patients with type diabetes mellitus” including goals: Investigating the expression levels of micro-RNA 29a, micro-RNA 146a, micro-RNA 147b in plasma in patients with type diabetes mellitus Evaluating the relationship between the expression level of micro-RNA 29a, micro-RNA 146a, micro-RNA 147b in plasma with some clinical, subclinical, insulin resistance status in patients with type diabetes mellitus * New contribution of the thesis This is the first study on the expression levels, association and diagnostic values of circulating miR-29a, miR-146a, miR-147b in plasma of patients with T2DM in Vietnam The results showed that the expression of circulating miR-29a and miR-147b in plasma significantly increased in patients with T2DM compared with healthy people (p0.05) There are positive correlation between plasma miR-29a and miR-147b expression levels with glucose concentration, HbA1C, and inversely correlated with C-peptide concentration and HOMA-β indexes The expression level of miR-146a was significantly lower while that of miR-147b was significantly higher in patients with T2DM with microalbuminuria in comparison to patients withous microalbuminuria (p0.05), the rate of hypertension accounted for 47.6% The average blood glucose concentration was 9.47 ± 2.67 mmol/l, HbA1C 8.2 ± 1.99 %, insulin 10.07 ± 5.53 µU/ml The rate of patients with microalbuminuria (+) was 66.7% The researchers had HOMA-IR 1.61 ± 0.59 ,which is higher than the control group 1.45 ± 0.42 (p