formosanus cua ciing td trong 1 dia petri trong dd dd chi cd mpt ti le nhd e i thi da dugc phoi nhiem trudc ydi chi pham Metavina 90DP, theo ddi sd moi chit do benh nim d Id thi nghie[r]
(1)PHAN II - XAY DUNG VA B A O VE CONG TRINH 625
KET QUA NGHIEN CLFU SLP DUNG CHE P H A M
METAVINA 90DP DE DIET TRLf M | COPTOTERMES
FORMOSANUS (ISOPTERA: RHINOTERMITIDAE) HAI CONG TRiNH KIEN TRUC
TS Trinh Vdn Hgnh"' Dinh Xudn Tudn Summary:
Research on usage of Metavina 90DP to treat Coptotermes formosanus (Isoptera: Rhinotermitidae) damaging buildings construction
Some results in the research process show that the strain Ma 01 can cause death all individuals of termite population with 1000-4000 individules in which 10-20% ones have innitially contaminated Culture and form features of the strain MaCI is still stable after re-isolation.In the preservation condition at 4°C, decline level of termite treatment of MaC1 is 2,9% and 8,3% after 12 months
I DAT V A N Dt
Mdi C formosanus la loai gay hai chii yeu cho cdng trinh kien tnic de diet trii' chiing ngudi ta thudng sir dung phucmg phap lay nhiem vdi cac hoa chat dang bpt, cac hoa chit la nhung chit dpc hai vdi ngudi va mdi trudng sinh thai Tir cac ket qua nghien ciru chiing tdi da xay dyng hoan thien quy trinh va san xuat cdng che phim Metavina 90DP cd ham lugng bao tir rat cao (10* - 10"'bt/g) De sir dung che pham Metavina 90DP cd hieu qua chiing tdi tien hanh nghien ciai sir dung che pham tren cic md hinh thi nghiem vdi sd lupng 1000 - 4000 ca the mdi
II VAT LIEU VA PHU'aNG PHAP NGHIEN ClJU ; Vdt lieu
To mdi Coptotermes formosanus Shiraki dugc nudi tai Trung tam nghien ciiu phdng trii mdi
Chi phim Metavina 90DP Trung tam nghien cim phdng trir mdi san xuat tir cac chiing nim A/ Anisopliae MaCl va MaC2
Hop nhir hop nhya de nudi mdi, dng nhya trang cd = 1,5 cm
(2)2 Phwffng phdp nghiin ciru
Thi nghiem tren dTa petri: Tha 50 c i thi moi C formosanus cua ciing td dia petri dd dd chi cd mpt ti le nhd e i thi da dugc phoi nhiem trudc ydi chi pham Metavina 90DP, theo ddi sd moi chit benh nim d Id thi nghiem v i sd mdi chet ty nhien d Id ddi chirng dl riit nhan xet, thi nghiem dugc lip lai lan
Thi nghiem vdi cac hop nudi: Mdi dugc nudi hop nhya da dugc bd sung giay cac tdng sau 15 mdi tao nen hop nhya hinh dang td gin gidng nhu vdi td phy Tir hop nhya ed cac ong nhira dan sang hop nhir mdi (so bd tri thi nghiem theo hinh I) Sau moi da an diy cac hop nhir, thi tiin hanh bam bpt che pham Metavina 90DP len minh cac ca thi mdi nhu tiin hanh lay nhiem bing hoi chit [I] Ket qui dugc dinh gii sau dd va kilm tra mirc dp sdng sdt ciia mdi d hop kiem tra va thiing dyng mdi sau 20 thi nghiem
Xir ly so lieu thu duge theo cdng thirc Abbot
Hop nuoi moi
Hinh Md hinh khdo nghiim thudc bdng phwffng phdp lay nhiim
Nhudm mdi mau dd de danh dau cic ca the mdi nhiem che pham ban dau bang Sudan Red 7B theo phuang phip ciia Lai, 1983 [ 3]
Che pham dugc bio qua d dieu kien 4*'C v i dieu kien nhiet phdng tir 25 den 30"C, de kiem tra hieu lyc diet mdi cua che pham theo thdi gian d cic dieu kien b i o qua khac
III K^T QUA NGHIEN CLFU
/ Xdc djnh ty li mdi bj nhiim chi phdm Metavina 90DP phong thi nghiim Cho mdi nhiem che phim Metavina 90DP vdi sd lugng khic nhau, rdi bd sung trd lai vao cac dia petri da cd mdi ban dau cho sd lugng mdi cic dia diu la 50 cac the Sau theo ddi ty le chet ciia mdi d cic dTa thi nghiem v i ty le mdi ehet ty nhien d cac dTa ddi chimg de riit nhan xet Sd lieu thi nghiem duge trinh b i y tren bang
(3)PHAN II - XAY DIJNG VA BAO VE CONG TRINH 627
bao tir miu xanh dim die trung ciia M anisopliae Trong dd xac nhirng mdi chet d Id ddi chung khdng cd bieu hien ciia benh nhiem nim va chiing bi vi khuin phan huy rat nhanh Dieu chimg td nhd tap tinh xa hpi ciia mdi, bao tu nim tir mpt sd mdi nhiem bao tir nam M anisopliae ban diu da lan truyen sang tat ca cac mdi khac dTa thi nghiem dc gay chit cho c i nhdm moi Kit qua phan tich cho thay chiing hai chiing MaCl va MaC2 cd hieu lire diet manh
Bang Ty le (%) moi chet ldy nhiem che phdm Metavina 90DP tren dia petri theo thai gian thi nghiem
Chiing giong cua ch6 pham Metavina 90DP (MaCl) Metavina 90DP (MaC2)
^r"^Ooi chu-ng "
Tile nhiem ban diu 12 12 4,0 ±1,0 2,5 ±1,2 2,25 ±1,1 8,0 ± 1,1 0,7 ±0,3
Ty le {%)
2 8,3 ±1,5 16,3 ±3,2 14,3 ±2,2 4,3 +1,2 12,3 ±2,4 20,8+2,9 1,3 ±0,3
moi chet theo thai • ' , " ~ !
j ; i hi
-14,5 49,0 ±1,8 ±5,0
55,5 ±5,0 31,5 81,3 ' 86,3 j ±3,1 ; ±3,6 \ ±3,9 , 28,3 87,5 , 93,8 ±4,8 : ±4,0 i ±4,0 10,5 , 38,0 57,5 : ±2,2 ±3.5 ±4,0 ; 46.3 : 82,0 : 87,8 : ±5,8 ; ±5,9 j ±4,8 j 53,0±5,
9 2,3
62,5±4, ! 96,3± 1,9 2,7 2,7 i ±0,3 ±0,7 i ±0,6 j
gian (ngay) 60,0 ! ±5,1 i 90,5 ±2,7 100 74,0 ±3,5 96,3 ±2,8 100 3,4 ±0,3 : 88,0 ±4,3 93,3 ±2,7 87,8 ±4,6 100 4,0 ±0,6 93 ! ±4,3 100 95,5 ±3,3 5,7 ±0,9 100 100 6,0 ±0,6 Sd lieu tren bang cdn cho thay tang ty le c i the moi bi nhiem bao tir M
anisopliae ban diu thi thdi gian mdi chit dat 100% giam di rd ret Vdi ty le mdi bi
nhilm bao tir ban diu la 4%, so mdi chet 100% la ngay; vdi 8% sd mdi chit 100% la - ngay; vdi 12% sd mdi chit 100% la
2 Thi nghiim ldy nhiim chung MaCl trin md hinh v&i 1000 - 4000 cd the mdi
(4)nhiem bao tir vdi sd lugng mdi tir 1000 - 4000 c i the mdi Vdi so lugng nay, mdi cd the tao nen td phu vat lieu cac tdng Kit qua thi nghiem dugc trinh bay d bing
Bdng Miii quan he giua «5 ngdy mdi chil 100% vai sd lugng mdi thi nghiem, giica ty le cd thi mdi hi nhiim hdo lu ban ddu vai dp ddi qudng duang mdi di chuyen
STT 10 11 12 13 14 15 16 17
So lupng m6i ; Ty le nhiim bao tif (ca the)
1000
2000
4000
ban dau (%)
10
20
10
20
10
20
Chiiu dii ong noi (cm)
100
S mdi j chit 0 %
10,4±1,07 \
300 11,4±0,68 500 14,8±0,66 100 8,0±0,32 300 j 8,8±0,37 j 500 i 9,0±0,45 100 14,8±0,86 i 300 r 15,4±0,81 I 500 ! 17,4±0,68 100 9,8±0.58 300 i 10,2±0,37
500 100
11,0±0,32 i >20 ; 300 j >20 500 i >20 i 100 11,4±0,51 300 '. 11,8±0,66
500 17,0+1,05
(5)PHAN II - XAY DIJNG VA BAO VE CONG TRINH 629 d thi nghiem tren dia Nhu vay, vdi sd lugng hang trieu c i thi mdt td mdi d ty nhien cin phii Iiy nhiem d ty le cao ban
3 Hiiu Iwc diet mdi mdi C formosanus eda cdc chung MaCI vd MaC2 tdi phdn lap
Mdt cie tieu chi ciia viec tuyen chpn cic chiing nim gay benh la hieu lyc ciia chiing phii dn dinh qui trinh lay nhiem [2] Chiing tdi da tiin hanh tii phan lap cic chiing MaCl va MaC2 tir xic mdi ehit bi phai nhilm bio tir ciia cie chiing niy de xic dinh lai hieu lyc cua cic ehimg d the he sau Kit q u i thi nghiem tren dTa petri duge trinh b i y tren bang
Bdng LT50 (gia) cita cdc chimg MaCl vd MaC2 tdi phdn lap
Chiing giong
MaCI MaC2
Lieu luang bio tu 5.10'
63,00 ±4,41 65,75 ± 2,42
5.10^ 91,25 ±4,27 96,25 ±3,77
5.10^ 134,75 ±3,63 143,00 ±5,08
5.10' >240 >240
06i chifng >240 >240
Sd lieu cho thay, sau tii phan lap, hieu lyc diet mdi ciia cic chiing MaC va MaC2 k h i dn dinh, cho phoi nhiem bao tir eiia cic ehiing tii phin lap d cic lieu lugng khic nhau, hieu lyc diet mdi dugc the hien qua LT50 tren bang Chimg td hieu lyc diet mdi cua chiing khdng thay ddi so vdi chiing gdc ban dau Dieu md trien vpng cic the he nam va bio tir ke tiep sinh tren xac mdi se tao nen sy lay nhiem mang tinh diy tmyen, lam cho hieu qui diet mdi se cao ban
4 Hiiu qud diet mdi C formosanus qud trinh bdo qudn bdo tir
Dinh ky mdi thing mdt lin qui trinh bao quin, liy bao tir xic dinh hieu qui diet mdi sau 72 gid xir ly bing dich huyen phii bao tir, vdi lieu lupng 10 /ml va lieu diing la 10ml, phoi nhiem cho 50 ca the mdi C formosanus dTa petri (10 xl5cm) Hieu qua diet mdi cua chiing MaC I trudc bao quan dugc xic dinh l i 67,9%, tuang ty, chiing MaC2 la 67,1 % Kit qui xic dinh hieu lyc dinh ky sau bio quan dugc trinh bay d bang
(6)Bdng Hieu qud diet mdi C formosanus Irong qud trinh bdo qudn bdo tic a cdc diiu kien khdc ciia chiing MaClvd MaC2
Thdi gian bao quan (thang)
1 10 11 12
Chung MaCI 25 - 30°C
67,3±0,76 67,0±0,43 66,2±0,43 64,8±0,71 60,8+0,53 57,8±0,08 56,2+0,51 53,3±0,65 50,6±0,51 47,5±0,67 43,5±0,90 39,4+0,34
Nhiet dp 4''C 67,6±0,21 67,3±0,24 66,7±0,15 65,6±0,14 65,4±0,10 65,0±0,10 64,4±0,07 63,5+0,03 62,20,12 61,6±0,15 60,4±0,20 59,6±0,31
Chung MaC2 25 - SO^C
66,8±0,24 66,6±0,24 66,1±0,20 64,8+0,70 60,5±0,42 57,6+0,16 56,0±0,45 52,9±0,50 50,3±0,42 46,9±0,55 43,5±0,31 39,4±0,32
Nhiet dp 4°C 66,8±0,15 66,6±0,26 65,7±0,15 65,4±0,22 64,6±0,25 64,0±0,29 63,4±0,12 62,3±0,18 60,9±0,22 61,5±0,32 60,3±0,14 57,3±0,24 IV K^T LUAN
1 Chiing nim MaCl cd ning gay chet cho tat ci cac c i the khac quin the cd sd lugng tu 1000 - 4.000 ci the vdi ty Ie lay nhiem ban diu li 10 -20% sd ci thi Khi ning gay benh lim chet din mdi C formosanus ciia bao tir chiing MaCl phy thudc vao ty le ca the mdi bi giy nhiem ban dau, ning gay chit xir Iy chi phim phu thupc vao khoing each giira nai giy nhiem vi nai tap tmng mdi
2 Sau tii phan lap tir cic ci the mdi bi nhiem benh, hieu luc diet mdi cua cic chiing MaCl va MaC2 la dn dinh
3 Miic dp suy giim hieu qua diet mdi cua cic chung MaCI v i MaC2 chi phim Metavina 90DP d diiu kien bao quin 4°C chi la 2,9% vi 8,3% sau 12 thing
TAI LIEU THAM KHAO
[1] Nguyen Chi Thanh, 1996 Nghien ciiu bien phip diet va phdng mdi khdng phai tim td cho cdng trinh xay dyng Luan an Phd Tien sy khoa hpc ndng nghiep, 166tr [2] Hanel, H., 1982 Biological control of the termite Nasutitermes axitiosus with
Metarhizium anisopliae Proc Biol Of Social Insect, pp 47