Sử dụng nước thải ao nuôi cá tra

TRƯỜNG ĐẠI HỌC AN GIANG KHOA NÔNG NGHIỆP – TÀI NGUYÊN THIÊN NHIÊN CHLORELLA VULGARIS BRACHIONUS PLICATILIS 2015 TRƯỜNG ĐẠI HỌC AN GIANG KHOA NÔNG NGHIỆP – TÀI NGUYÊN THIÊN NHIÊN CHLORELLA VULGARIS BRACHIONUS PLICATILIS NĂM 2015 Chlorella vulgaris Brachionus plicatilis.” N2 TS Chau Thi Đa i Chlorella vulgaris – ối tượ Brachionus plicatilis Mật độ tảo yếu tố môi trường pH, nhiệt độ theo dõi hàng ngày, yếu tố khác NH4+, NO3-, PO43-, TSS, COD, BOD đo ngày/lần - êm NH4+ 384449 ct/mL Brachionus plicatilis, 3- + Chlorella vulgaris ii - PO43- ABSTRACT Evaluation the ability to use waste water from striped catfish ponds to raise Chlorella sp and rotifers Brachionus sp biomass was implemented from June to August 2014 at the experimental area of An Giang University The experiment was arranged in a completely randomized design on species with treatments and replications for each species in 10 days Treatments were 100% waste water from striped catfish pond and algae density were 100,000 individual/mL, 200,000 individual/mL, 300,000 individual/mL and 400,000 individual/mL; or rotifer density were 30 individual/mL, 50 individual/mL, 75 individual/mL and 100 individual/mL The density of algae, rotifers as well as environmental factors as pH, temperature was monitored daily, while other factors such as NH4+, NO3-, PO43-, TSS, COD, BOD was measured days/times The results showed that the temperature and pH were appropriate for the growth of Chlorella sp and Brachionus sp The other environmental factors were markedly improved after the experiment; these environmental factors were adapted standard column A2 or B1 according to QCVN in algae experiment However, in rotifers experimental was only NO3- factor decreased 44.62% (column A1), COD decreased 67.91% (column A2), BOD dropped 72.98% (column A2), TSS dropped 93.62% (column B1) while the other factors such as NH4+ and PO43- were unqualified and need to more treat Algae density peaked on day in treatment NT4 was 5,811,111 individual/mL with the ability to reduce the level of nutrients concentrate NH4+, NO3- and PO43in wastewater were 88.19%, 91.16% and 67.72%, respectively Rotifer populations also peaked on day was 833,330 individual/L in treatment NT7 and the highest growth rate was 0.9%/day in treatment NT8 Keywords: Brachionus sp., striped catfish, Chlorella sp., Rotifers, waste water, algae biomass iii iv năm 2015 Trang 1 2 2 3 3 3 4 6 6 7 10 10 10 10 10 10 10 10 10 10 10 11 14 1.2 CHƯƠNG 2: 2.1.6 3.1.2 v 4 + , NO3-, PO43- + , NO3-, PO43- vi 15 15 15 15 15 17 19 19 21 22 23 23 23 23 24 26 26 28 28 29 31 31 32 32 35 Trang 4 + + + + 34 4 NT3 vii 12 19 20 20 20 20 20 20 21 21 27 29 29 30 30 31 Trang + 15 16 17 17 18 19 21 22 23 24 24 25 25 25 26 28 28 29 - 34 + 34 viii treat N Mean Grouping NT1 305555 A NT2 NT4 3 255555 238889 A A NT3 194444 A Grouping Information Using Tukey Method and 95.0% Confidence for day treat NT2 NT1 N 3 Mean 311111 261111 Grouping A A NT4 NT3 3 205556 144444 A A Grouping Information Using Tukey Method and 95.0% Confidence for day treat NT1 N Mean 522222 Grouping A NT2 NT3 3 450000 444444 A A NT4 288889 A Grouping Information Using Tukey Method and 95.0% Confidence for 10 day treat N Mean Grouping NT2 NT1 3 1172222 A 961111 A NT3 NT4 3 700000 450000 A A Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT1 1.1262 A NT2 NT3 3 1.0204 0.7214 A A NT4 0.6720 A Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT2 NT1 3 1.2150 1.1810 A A NT4 NT3 3 0.8838 0.8145 A A 43 Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT1 NT2 NT4 3 0.9658 0.8683 0.8312 A B B NT3 0.8267 B Grouping Information Using Tukey Method and 95.0% Confidence for day treat NT1 NT2 N 3 Mean 0.8557 0.7618 Grouping A B NT3 0.7409 B NT4 0.6672 C C Grouping Information Using Tukey Method and 95.0% Confidence for day treat NT1 N Mean 0.5728 Grouping A NT2 NT3 3 0.3600 0.3261 B B NT4 0.2590 B Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT1 0.4067 A NT2 0.1911 B NT3 NT4 3 0.1894 -0.1204 B C Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT1 0.1522 A NT2 0.0156 A NT3 -0.0621 B NT4 -0.0796 B B Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT1 0.1058 A NT2 NT4 3 0.0329 -0.0845 A B NT3 -0.0984 B B 44 Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT1 NT2 NT3 3 0.1718 0.0796 0.0420 A A A NT4 -0.0524 B B B Grouping Information Using Tukey Method and 95.0% Confidence for 10 day treat NT1 NT2 N 3 Mean 0.2186 0.1755 Grouping A A NT3 0.0690 A NT4 -0.0092 B B Grouping Information Using Tukey Method and 95.0% Confidence for day Treat N Mean Grouping NT1 NT3 3 6.5025 4.3531 A A NT2 NT4 3 2.7100 2.1917 A A Grouping Information Using Tukey Method and 95.0% Confidence for day Treat N Mean Grouping NT3 2.5141 A NT1 2.3524 A NT2 1.0773 A NT4 0.6418 A Grouping Information Using Tukey Method and 95.0% Confidence for day Treat N Mean Grouping NT1 NT2 NT3 3 7.1810 5.7769 4.0463 A A A NT4 3.2415 A Grouping Information Using Tukey Method and 95.0% Confidence for day Treat NT2 N Mean 7.7515 Grouping A NT1 6.3342 A NT3 5.9935 A NT4 3.2278 A 45 Grouping Information Using Tukey Method and 95.0% Confidence for day Treat N Mean Grouping NT2 NT4 NT3 3 18.9112 13.7706 8.2074 A A A NT1 5.3960 A Grouping Information Using Tukey Method and 95.0% Confidence for day Treat NT1 NT4 N 3 Mean 21.0049 16.9192 Grouping A A NT2 11.4815 A NT3 10.8887 A Grouping Information Using Tukey Method and 95.0% Confidence for day Treat NT3 N Mean 36.5151 Grouping A NT4 NT1 3 15.1281 14.9495 B B NT2 13.3333 B Grouping Information Using Tukey Method and 95.0% Confidence for day Treat N Mean Grouping NT3 19.6967 A NT1 19.2424 A NT2 NT4 3 18.6204 10.8423 A A Grouping Information Using Tukey Method and 95.0% Confidence for day Treat N Mean Grouping NT4 13.0159 A NT2 0.8772 A NT3 0.0000 A NT1 -0.0000 A Grouping Information Using Tukey Method and 95.0% Confidence for 10 day Treat N Mean Grouping NT2 1.4833 A NT4 NT1 3 0.6667 0.5128 A A NT3 0.0000 A + Grouping Information Using Tukey Method and 95.0% Confidence for day 46 treat N Mean Grouping NT8 6.133 A NT7 NT6 3 6.133 6.133 A A NT5 6.133 A Grouping Information Using Tukey Method and 95.0% Confidence for day treat NT8 NT7 N 3 Mean 5.827 5.704 Grouping A A NT5 NT6 3 5.520 5.091 A B Grouping Information Using Tukey Method and 95.0% Confidence for day treat NT7 N Mean 5.336 Grouping A NT8 NT5 3 5.311 5.091 A A NT6 4.907 B B Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT7 NT6 3 5.397 5.275 A A NT5 NT8 3 5.152 5.152 A A Grouping Information Using Tukey Method and 95.0% Confidence for day treat B4 N Mean 5.213 Grouping A B3 B2 3 5.213 5.213 A A B1 5.213 A Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT8 5.029 A NT7 5.029 A NT6 5.029 A NT5 5.029 A 34 Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping 47 NT6 1.444 A NT7 1.335 A NT8 NT5 3 1.275 1.154 A A Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT6 NT7 NT8 3 1.211 1.081 1.072 A A A NT5 1.031 A Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT7 NT8 3 1.795 1.771 A A NT6 NT5 3 1.607 1.408 A B B B Grouping Information Using Tukey Method and 95.0% Confidence for day treat NT7 N Mean 1.677 Grouping A NT8 NT6 3 1.506 1.446 A A NT5 1.294 A Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT8 NT6 3 2.182 1.765 A A NT7 NT5 3 1.661 1.426 A B B B Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT8 1.241 A NT7 1.239 A NT6 1.202 B NT5 1.181 B Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT8 25.777 A 48 NT7 25.777 A NT6 25.777 A NT5 25.504 A Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT6 24.080 A NT7 NT8 NT5 6 24.077 19.892 19.117 A A A Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT7 15.776 A NT6 NT8 6 14.848 14.752 A A NT5 14.662 A Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT7 NT6 6 20.413 17.080 A A NT8 NT5 15.067 12.880 A A Grouping Information Using Tukey Method and 95.0% Confidence for day treat NT8 N Mean 20.760 Grouping A NT7 NT6 6 17.160 16.560 A A NT5 6.048 B B Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT7 18.400 A NT8 16.200 A B NT6 15.333 A B NT5 12.880 B Grouping Information Using Tukey Method and 95.0% Confidence for day treat NT8 N Mean 15.466 Grouping A NT7 15.466 A 49 NT6 15.466 A NT5 15.466 A Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT5 14.383 A NT6 13.982 A NT7 NT8 3 13.618 13.301 A A Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT6 13.919 A NT5 13.610 A NT7 NT8 3 13.431 12.373 A A Grouping Information Using Tukey Method and 95.0% Confidence for day treat NT6 N Mean 10.413 Grouping A NT5 NT7 3 9.880 9.413 A A NT8 9.067 A Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT6 NT8 3 8.893 8.093 A A NT7 NT5 3 7.493 6.240 A A Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT7 15.067 A NT6 13.000 A B NT8 11.533 A B NT5 9.547 B ngay1 Duncan NT N Subset for alpha = 05 50 6.00 61.1100 8.00 62.2233 7.00 67.7800 5.00 71.1133 Sig .486 Means for groups in homogeneous subsets are displayed a Uses Harmonic Mean Sample Size = 3.000 ngay3 Duncan Subset for alpha = 05 NT N 6.00 27.7767 5.00 40.0033 8.00 7.00 40.0033 44.4433 52.2200 Sig .079 090 Means for groups in homogeneous subsets are displayed a Uses Harmonic Mean Sample Size = 3.000 ngay5 Duncan Subset for alpha = 05 NT N 6.00 18.6667 5.00 22.8900 8.00 7.00 Sig 38.6667 41.3333 497 665 Means for groups in homogeneous subsets are displayed a Uses Harmonic Mean Sample Size = 3.000 ngay7 Duncan NT N Subset for alpha = 51 .05 6.00 10.0000 5.00 10.8900 8.00 12.2200 7.00 18.8900 Sig .084 Means for groups in homogeneous subsets are displayed a Uses Harmonic Mean Sample Size = 3.000 ngay9 Duncan Subset for alpha = 05 NT N 5.00 7.00 7.7800 8.00 10.0000 6.00 12.2233 7.7800 Sig .400 Means for groups in homogeneous subsets are displayed a Uses Harmonic Mean Sample Size = 3.000 Grouping Information Using Tukey Method and 95.0% Confidence for day treat NT8 NT7 N 3 Mean Grouping 245832 A 146010 B NT6 NT5 3 84997 49446 C D Grouping Information Using Tukey Method and 95.0% Confidence for day treat NT8 N Mean Grouping 366666 A NT7 NT6 3 255556 B 141889 C NT5 81667 C Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT8 726333 A NT7 711108 A 52 NT6 501222 B NT5 299999 C Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT7 833330 A NT8 827778 A NT5 NT6 3 405553 B 377768 B Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT8 600000 A NT7 378889 A B NT6 NT5 3 316666 A 183333 B B Grouping Information Using Tukey Method and 95.0% Confidence for day treat NT8 N Mean Grouping 382947 A NT7 NT6 3 345213 A 189840 B NT5 122412 C B C Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT8 NT7 3 150000 A 127774 A NT6 NT5 3 84444 75444 A A Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT8 120000 A NT7 102222 A NT6 47778 A NT5 43889 A Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT8 81667 A NT7 NT6 3 48889 33333 B B NT5 29444 B 53 Grouping Information Using Tukey Method and 95.0% Confidence for 10 day treat N Mean Grouping NT5 NT8 NT6 3 228333 A 64444 A 48333 A NT7 44286 A Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT8 2000.00 A NT5 1000.00 A NT7 333.33 A NT6 333.33 A Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT6 NT8 3 1666.67 1000.00 A A NT5 NT7 3 666.67 333.33 A A Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT7 666.67 A NT5 666.67 A NT6 333.33 A NT8 333.33 A Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT5 NT7 NT6 3 1000.00 666.67 333.33 A A A NT8 333.33 A Grouping Information Using Tukey Method and 95.0% Confidence for day treat NT6 N Mean 1333.33 Grouping A NT7 666.67 A NT5 666.67 A NT8 333.33 A Grouping Information Using Tukey Method and 95.0% Confidence for day 54 treat N Mean Grouping NT7 1000.00 A NT5 NT6 3 1000.00 333.33 A A NT8 0.00 A Grouping Information Using Tukey Method and 95.0% Confidence for day treat NT5 NT6 N 3 Mean 1666.67 333.33 Grouping A A NT8 NT7 3 333.33 0.00 A A Grouping Information Using Tukey Method and 95.0% Confidence for day treat NT7 N Mean 666.67 Grouping A NT6 NT8 3 666.67 333.33 A A NT5 333.33 A Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT8 NT6 3 1000.00 666.67 A A NT7 NT5 3 0.00 -0.00 A A Grouping Information Using Tukey Method and 95.0% Confidence for 10 day treat NT8 N Mean 666.67 Grouping A NT5 NT7 3 666.67 333.33 A A NT6 333.33 A Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT6 NT7 3 44.7347 38.6617 A A NT5 NT8 3 32.4897 31.2765 A A Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping 55 NT5 39.8293 A NT7 32.3689 A NT8 NT6 3 29.7017 25.7799 A A Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT8 NT6 NT5 3 25.8594 19.1086 17.6785 A A A NT7 9.7474 B B B Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT8 NT5 3 23.9920 15.1009 A B NT6 NT7 3 15.0801 10.9872 B C Grouping Information Using Tukey Method and 95.0% Confidence for day treat NT5 N Mean 22.3420 Grouping A NT6 NT8 3 16.4081 15.5828 B B NT7 11.0853 C C Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT5 NT6 3 25.3204 17.7683 A A NT8 NT7 3 12.8089 7.0295 A B B B Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT7 16.6673 A NT5 8.3333 A NT6 8.3333 A NT8 3.0303 A Grouping Information Using Tukey Method and 95.0% Confidence for day treat NT6 N Mean 68.6728 Grouping A NT8 11.1111 A 56 NT5 0.0000 A NT7 -0.0000 A Grouping Information Using Tukey Method and 95.0% Confidence for day treat N Mean Grouping NT5 2.6667 A NT7 0.7143 A NT8 NT6 3 0.4000 0.0000 A A Grouping Information Using Tukey Method and 95.0% Confidence for 10 day treat N Mean Grouping NT7 1.1709 A NT5 1.0753 A NT6 NT8 3 0.8730 -0.0000 A A 57 ... thải cá tra Nước thải đượ trực tiếp từ ao nuôi cá tra TP Long Xuyên, An Giang Ao nuôi cá tra khoảng 5,5 tháng, nước ao xả c 10 3.3.2.1 Cách xử lý Đối với thí nghiệm ni tảo Chlorella: - Nước thải. .. hệ thống nuôi cá sử dụng thức ăn tự chế thức ăn tươi từ xác cá tra cao cao gấp - 10 lần so với hệ thống nuôi sử dụng thức ăn viên Qua cho thấy, sử dụng nguồn thức ăn tự chế nuôi cá tra tăng lượng... nhiễm khác Các nghiệm thức bố trí với hàm lượng mật độ sau: Nghiệm thức (NT1): 100% nước thải ao nuôi cá tra + mật số tảo 100.000 ct/ml Nghiệm thức (NT2): 100% nước thải ao nuôi cá tra + mật số