Phân lập tuyển chọn vi sinh vật xử lý loại bỏ nitơ trong nước thải chế biến thủy sản

LUẬN VĂN THẠC SĨ CHUYÊN NGÀNH: KỸ THUẬT MÔI TRƢỜNG MÃ NGÀNH: 60520320 HVCH: HUỲNH VĂN THÀNH HDKH: TS NGUYỄN HỒI HƢƠNG Tp Hồ Chí Minh, tháng năm 2013 TỔNG QUAN VẬT LIỆU VÀ PHƢƠNG PHÁP NGHIÊN CỨU KẾT QUẢ THẢO LUẬN KẾT LUẬN, KIẾN NGHỊ TỔNG QUAN Nƣớc thải chế biến thủy sản (CBTS) Công nghệ xử lý nƣớc thải CBTS VN Vấn đề tồn Chiến lƣợc xử lý nƣớc thải CBTS Cá da trơn: 5-7 m3/tấn sản phẩm Tôm đông lạnh: 4-6 m3/tấn sản phẩm Surimi: 20-25 m3/tấn sản phẩm Thuỷ sản đông lạnh hỗn hợp: 4-6 m3/tấn sản phẩm (Nguồn: Tổng cục Môi trường 2011) (Tổng cục Môi trường, 2011) Nước thải Bể khử trùng Nguồn tiếp nhận Song chắn rác Ngăn thu gom Bể lắng Bể chứa bùn Bùn thải Bể điều hịa Bùn hoạt tính hiếu khí Tuần hồn bùn Phân hủy hiếu khí Amon hóa Nitrate hóa Nước thải Mương tách dầu mỡ Máy tách rác Thiết bị lược rác tinh Bể tiếp nhận Bể điều hịa Bể tạo bơng Bể tuyển Bể sinh học BHTDB Bể sinh hoc BHTLL Bể anoxic Bể lắng Bể trung gian Bể lọc áp lực Phân hủy hiếu khí, amon hóa, nitrate hóa Nguồn tiếp nhận Bể khử trùng Phân hủy thiếu khí Nước thải Bể khử trùng Nguồn tiếp nhận Song chắn rác Ngăn thu gom Bể lắng Bể sinh học hiếu khí Bể điều hịa Bể kỵ khí Phân hủy kị khí Bể chứa bùn Tuần hồn bùn Bùn thải Nitrate hóa Nước thải CBTS • Giàu protein Xử lý kị khí và/hoặc hiếu khí • Protein Amino acid NH4+ NO2- NO3- Nước thải đầu • Hàm lượng N cao dạng NH4+ NO2- NO3- Nitrate hóa • NH4+ bán/tồn phần Phản nitrate Nitrate hóa bán phần Anammox NO2- NO3- • NO3- NO2- NO • NH4+ NO2- • NH4+ + NO2- N2O N2 N2 + H2O Muốn hiệu cao, phải tăng cường sinh học = cung cấp đầy đủ VSV cần thiết để loại bỏ triệt để hợp chất N Bảng 5: số liệu khảo sát mật độ vsv thích hợp NO3- NO2- NH4+ Thời gian 10^5 10^6 10^7 10^8 10^5 10^6 10^7 10^8 10^ 0.495 0.488 0.497 0.48 0.001 0.001 0.006 0.032 0 0.517 0.502 0.493 0.476 0.001 0.001 0.002 0 0.506 0.495 0.495 0.478 0.001 0.001 0.004 0.516 0.496 0.484 0.288 0.004 0.005 0.016 0.035 0.033 0.52 0.492 0.504 12 12 0.303 0.295 0.065 0.069 0.005 0.004 0.001 0.001 0.467 0.406 0.067 0.001 0.005 18 18 0.411 0.438 0.424 0.456 0.438 0.487 0.485 0.425 0.387 0.005 0.503 0.499 0.461 0.473 0.405 0.405 0.226 0.21 0.004 0.004 18 0.447 0.405 0.218 24 24 0.474 0.474 0.404 0.418 0.107 0.131 0.004 0.001 0.002 0.004 0.004 24 0.474 0.411 0.119 0.004 0.027 36 36 0.398 0.385 0.391 0.067 0.06 0.063 0.144 0.133 0.138 0.004 0.004 0.008 0.005 0.006 0.001 0.000 0.001 0.001 0.097 0.097 0.085 0.08 0.082 0.121 0.116 0.118 0.389 0.373 0.015 0.015 0.085 0.084 0.084 0.418 0.413 0.415 0.792 0.78 0.001 0.097 0.002 0.002 0.002 12 36 48 48 48 0.004 0.01 0.00 10^ 0.0 0.0 0 0 0 0 0 0 0 0.007 0.009 0 0.008 0 0 0 0 0 0 0 0 0 0.456 0.448 0.511 0.515 0.51 0.505 0.507 0.524 0.507 0.515 0.371 0.35 0.360 0.006 0.004 0 0 0 0.004 0.015 0.009 0.002 0.011 0.006 0 0.381 0.452 0.005 0 0 0.749 0.729 0.48 0.476 0.364 0.35 0 0 0 0 0.739 0.478 0.357 0.244 0.217 0.230 0 0 0.004 0.028 0.026 0.005 0.006 0.004 0.786 10^6 10^8 0.011 0.005 0.008 Đường chuẩn phosphate 0.25 y = 0.8371x - 0.0035 R² = 0.9976 0.2 0.15 Series1 0.1 Linear (Series1) Linear (Series1) 0.05 0 0.05 0.1 0.15 0.2 0.25 0.3 -0.05 Đường chuẩn nitrate 1.6 y = 0.5346x + 0.0463 R² = 0.9937 1.4 1.2 Series1 0.8 Linear (Series1) 0.6 0.4 0.2 0 0.5 1.5 2.5 Đường chuẩn nitrate 1.6 y = 0.5346x + 0.0463 R² = 0.9937 1.4 1.2 Series1 0.8 Linear (Series1) 0.6 0.4 0.2 0 0.5 1.5 2.5 Đường chuẩn ammonium 0.18 0.16 y = 0.1697x - 0.0005 R² = 0.9989 0.14 0.12 OD 0.1 0.08 Series1 0.06 Linear (Series1) 0.04 0.02 -0.02 0.2 0.4 0.6 N-NH3 0.8 1.2 TN Nguồn dinh dưỡng C N MT1, Biến đổi N (mg/L), OD600nm, hiệu xử lý nitrate theo thời gian MT1 Thời Efficiency, N-NO3N-NO2N-NH4+ OD gian % 227,42 3,22 32,33406 0,093 0,00% 194,5763 10,83604 31,41895 0,091 9,94% 142,0339 39,11255 34,46933 0,09 18,01% 133,1356 41,93032 35,38444 0,096 19,97% 12 148,6017 44,90627 31,72398 0,097 14,35% 18 96,27119 65,56988 35,38444 0,094 25,00% 24 95,42373 70,03876 28,97864 0,105 26,06% 48 12,10251 107,4112 44,53559 0,107 37,62% 96 108,5779 4,880613 0,135 56,86% MT2, Biến đổi N (mg/L), OD600nm, hiệu xử lý nitrate theo thời gian MT2 Thời Efficiency, N-NO3N-NO2N-NH4+ OD gian % 219,5763 3,371431 37,51971 0,061 0,00% 215,339 10,6383 31,41895 0,079 1,18% 138,8559 46,37942 30,19879 0,089 17,29% 137,3729 41,58428 26,53833 0,077 21,11% 12 139,0678 39,17187 29,89375 0,078 20,09% 18 88,85593 57,8581 34,46933 0,094 30,44% 24 89,27966 65,68852 38,12979 0,083 25,86% 48 1,814441 115,7162 30,50383 0,144 43,17% 96 42,92889 3,050383 0,120 82,35% MT3, Biến đổi N (mg/L), OD600nm, hiệu xử lý nitrate theo thời gian MT3 Thời gian 12 18 24 48 96 N-N 3- N-NO2- N-NH4+ OD 213,4322 192,6695 86,31356 65,76271 89,70339 27,20339 21,27119 0 4,112948 11,92359 65,16452 73,32121 64,38345 100,2729 123,2302 97,52432 22,87787 23,79299 18,91237 20,13253 25,62322 30,19879 24,09803 45,14567 10,98138 0,056 0,08 0,105 0,112 0,113 0,112 0,112 0,123 0,14 2.Thí nghiệm khảo sát tỉ lệ C:N N-NO3-, mg/L Thời gian C/N=4 C/N=12 C/N=18 164,07 164,07 164,07 68,94 67,03 52,84 12 29,53 10,04 0,00 18 0 24 0 36 0 48 0 72 0 N-NO2-, mg/L Thơi gian C/N=4 C/N=12 C/N=18 19,29 17,16 24,08 57,35 63,43 76,63 12 78,32 108,78 126,28 18 125,39 126,47 125,19 24 117,38 118,56 111,35 36 75,85 77,43 72,79 48 101,95 93,75 83,47 72 56,67 58,45 71,90 N-NH4+, mg/L Thơi gian C/N=4 C/N=12 C/N=18 15,862 11,591 14,947 6,711 5,491 20,743 12 0,305 0,305 0,305 18 0,305 0,305 0,305 24 0,305 0,305 0,305 36 0,305 0,305 0,305 48 0,305 0,305 0,305 72 0,305 0,305 0,305 Efficiency, % 0,00% 5,01% 29,13% 33,78% 25,25% 34,42% 29,87% 40,66% 95,43% Hiệu xử lý nitrate (%) Thơi gian C/N=4 C/N=12 C/N=18 0% 0% 0% 33% 29% 26% 12 46% 38% 38% 18 37% 34% 38% 24 41% 38% 45% 36 62% 60% 64% 48 49% 51% 59% 72 71% 70% 64% Thí nghiệm khảo sát nồng độ NaCl vi khuẩn chịu NaCl 0% NaCl 3% NaCl 5% NaCl 7% 0,148 0,22 0,23 0,234 0,184 0,223 0,181 0,178 12 0,296 0,357 0,167 0,101 18 0,336 0,339 0,184 0,192 24 0,381 0,395 0,184 0,151 36 0,327 0,391 0,131 0,168 48 0,335 0,469 0,141 0,125 72 0,337 0,388 0,127 0,128 Nồng độ muối 5% trở lên, VSV không tăng trưởng N-NO3mg/L Thời gian NaCl 0% NaCl 3% NaCl 5% NaCl 7% 172,12 145,85 181,86 164,49 35,04 108,98 158,35 135,47 12 26,99 107,08 121,48 177,63 18 10,25 98,60 107,08 145,21 24 2,63 22,75 162,37 104,96 36 0,00 11,74 157,29 175,72 48 0,00 14,70 119,79 167,25 72 0,00 3,69 137,37 148,39 96 0,00 0,00 105,38 157,29 N-NO2mg/L Thừoi gian NaCl 0% NaCl 3% NaCl 5% NaCl 7% 21,61 18,05 15,48 15,48 96,26 54,04 15,73 14,59 12 95,86 77,77 19,44 14,99 18 88,94 36,49 19,04 15,09 24 75,45 73,52 21,42 15,33 36 90,67 89,54 22,60 15,88 48 75,25 86,72 25,72 16,22 72 52,61 89,68 25,07 16,42 96 13,60 20,82 44,55 16,87 N-NH4+ mg/L Thời gian NaCl 0% NaCl 3% NaCl 5% NaCl 7% 0,31 0,31 0,31 0,31 0,31 0,31 0,31 0,31 12 17,39 5,19 0,31 0,31 18 8,85 8,85 0,31 0,31 24 14,34 18,91 0,31 0,31 36 19,22 10,98 10,68 11,59 48 14,34 7,93 6,71 8,85 72 6,71 10,68 15,56 17,39 96 6,71 6,10 7,32 7,93 Giờ 12 18 24 36 48 72 96 Hiệu xử lý NaCl 0% NaCl 3% NaCl 5% NaCl 7% 0% 0% 0% 0% 32% 1% 12% 17% 28% -16% 29% -7% 44% 12% 36% 11% 52% 30% 7% 33% 43% 32% 4% -13% 54% 33% 23% -7% 69% 37% 10% -1% 90% 84% 20% -1% Vai trò giá thể Thời gian 0h 6h 12h 18h 24h 36h 48h Thời gian 0h 6h 12h 18h 24h 36h 48h Thời gian 12 18 24 36 48 72 96 0% NaCl NO3- NO2- NH4+ Hiệu 164,28 7,13 0,31 0% 95,85 32,54 0,31 25% 0,00 51,13 0,31 70% 0,00 5,45 4,27 94% 0,00 0,00 0,31 100% 0,00 0,00 0,31 100% 0,00 0,00 0,31 100% 3%NaCl NO3- NO2- NH4+ Hiệu 138,43 6,04 0,31 0% 130,81 15,68 0,31 -1% 97,12 40,84 0,31 4% 80,38 24,88 3,36 25% 30,38 35,45 3,66 52% 0,00 21,86 3,97 82% 0,00 15,53 1,53 88% Hiệu xử lý NaCl 0%, giá thể NaCl 3%, giá thể NaCl 0% NaCl 3% 0% 0% 0% 0% 25% -1% 32% 1% 70% 4% 28% -16% 94% 25% 44% 12% 100% 52% 52% 30% 100% 82% 43% 32% 100% 88% 54% 33% 69% 37% 90% 84% Ảnh hưởng mật độ Thời gian 12 18 24 36 48 10^5 194,79 193,94 160,25 169,79 181,23 146,27 7,29 Thời gian 12 18 24 36 48 Thời gian 12 18 24 36 48 Thời gian 12 18 24 36 48 NO310^6 10^7 10^8 190,13 190,13 182,92 192,03 186,10 105,59 178,26 152,42 8,77 151,99 72,75 0,00 154,53 30,81 0,00 39,07 0,00 0,00 0,00 0,00 0,00 NO210^5 10^6 10^7 0,00 0,00 0,00 0,00 0,00 0,70 0,00 0,00 7,52 0,00 0,00 40,25 1,84 10,89 76,88 8,76 73,68 87,72 72,23 92,86 68,93 10^5 0,31 0,31 0,31 0,31 0,31 0,31 0,31 10^5 0% 0% 18% 13% 6% 20% 59% NH4+ 10^6 10^7 4,58 6,41 0,31 0,31 0,31 0,31 0,31 0,31 0,31 0,31 0,31 2,14 0,31 2,14 Hiệu 10^6 10^7 0% 0% 1% 5% 8% 18% 22% 42% 15% 45% 42% 54% 52% 64% 10^8 2,48 50,14 98,69 50,14 34,81 0,00 21,96 10^8 5,19 0,31 5,19 6,10 4,27 1,53 1,53 10^8 0% 18% 41% 70% 79% 99% 88% Nguồn C N (48h) Summary Statistics for hl nitrate moi truong Count Average Standard deviation Coeff of variation Minimum mt1 27.4167 1.275 4.65047% 26.14 mt2 5.0 5.49928 109.986% 0.0 mt3 0.0 0.0 % 0.0 Total 10.8056 12.9563 119.904% 0.0 Table of Means for hl nitrate by moi truong with 95.0 percent LSD intervals Stnd error moi truong Count Mean (pooled s) Lower limit Upper limit mt1 27.4167 1.88172 24.1609 30.6725 mt2 5.0 1.88172 1.74419 8.25581 mt3 0.0 1.88172 -3.25581 3.25581 Total 10.8056 Multiple Range Tests for hl nitrate by moi truong Method: 95.0 percent LSD moi truong Count Mean mt3 0.0 mt2 5.0 mt1 27.4167 Maximum 28.69 10.89 0.0 28.69 Range 2.55 10.89 0.0 28.69 Stnd skewness -0.00831883 0.501481 Maximum 109.39 117.3 99.9 117.3 Range 3.96 3.17 4.75 22.15 Stnd skewness 0.0 -0.00669182 0.00446593 -0.210359 Homogeneous Groups X X X Contrast Sig Difference +/- Limits mt1 - mt2 * 22.4167 6.51162 mt1 - mt3 * 27.4167 6.51162 mt2 - mt3 5.0 6.51162 * denotes a statistically significant difference Summary Statistics for hl nitrite B.moi truong Count Average Standard deviation mt1 107.41 1.98 mt2 115.717 1.585 mt3 97.5233 2.375 Total 106.883 8.0769 Coeff of variation 1.8434% 1.36973% 2.43532% 7.55675% Minimum 105.43 114.13 95.15 95.15 B.moi truong Stnd kurtosis mt1 mt2 mt3 Total -0.884204 ANOVA Table for hl nitrite by B.moi truong Source Sum of Squares Df Mean Square F-Ratio P-Value Between groups 497.744 248.872 61.84 0.0001 Within groups 24.1465 4.02442 Total (Corr.) 521.891 Table of Means for hl nitrite by B.moi truong with 95.0 percent LSD intervals Stnd error B.moi truong Count Mean (pooled s) Lower limit Upper limit mt1 107.41 1.15822 105.406 109.414 mt2 115.717 1.15822 113.713 117.721 mt3 97.5233 1.15822 95.5193 99.5273 Total 106.883 Multiple Range Tests for hl nitrite by B.moi truong Method: 95.0 percent LSD B.moi truong Count mt3 mt1 mt2 Mean 97.5233 107.41 115.717 Homogeneous Groups X X X Contrast Sig Difference +/- Limits mt1 - mt2 * -8.30667 4.00798 mt1 - mt3 * 9.88667 4.00798 mt2 - mt3 * 18.1933 4.00798 * denotes a statistically significant difference Summary Statistics for hl amoni 0.775217 C.moi truong mt1 mt2 mt3 Total Count 3 Average 44.5367 30.5033 45.1467 40.0622 Standard deviation 0.305014 0.915005 4.575 7.54533 Coeff of variation 0.68486% 2.99969% 10.1336% 18.834% Minimum 44.23 29.59 40.57 29.59 Maximum 44.84 31.42 49.72 49.72 Range 0.61 1.83 9.15 20.13 Stnd skewness -0.03477 0.0115917 -0.00231838 -0.625178 C.moi truong Stnd kurtosis mt1 mt2 mt3 Total -0.949213 ANOVA Table for hl amoni by C.moi truong Source Sum of Squares Df Mean Square F-Ratio P-Value Between groups 411.734 205.867 28.25 0.0009 Within groups 43.7218 7.28697 Total (Corr.) 455.456 Table of Means for hl amoni by C.moi truong with 95.0 percent LSD intervals Stnd error C.moi truong Count Mean (pooled s) Lower limit Upper limit mt1 44.5367 1.55852 41.8401 47.2333 mt2 30.5033 1.55852 27.8067 33.1999 mt3 45.1467 1.55852 42.4501 47.8433 Total 40.0622 Multiple Range Tests for hl amoni by C.moi truong Method: 95.0 percent LSD C.moi truong Count mt2 mt1 mt3 Mean 30.5033 44.5367 45.1467 Homogeneous Groups X X X Contrast Sig Difference +/- Limits mt1 - mt2 * 14.0333 5.39321 mt1 - mt3 -0.61 5.39321 mt2 - mt3 * -14.6433 5.39321 * denotes a statistically significant difference tỷ lệ cn (12h) Summary Statistics for hl nitrate ty le cn Count Average Standard deviation CN12 10.0433 3.815 CN18 0.0 0.0 CN4 29.5333 2.965 Total 13.1922 13.227 Coeff of variation 37.9854% % 10.0395% 100.264% Minimum 6.23 0.0 26.57 0.0 ty le cn Stnd kurtosis CN12 CN18 CN4 Total -1.00121 ANOVA Table for hl nitrate by ty le cn Source Sum of Squares Df Mean Square F-Ratio Between groups 1352.95 676.473 86.93 Within groups 46.6909 7.78182 Total (Corr.) 1399.64 Table of Means for hl nitrate by ty le cn with 95.0 percent LSD intervals Stnd error ty le cn Count Mean (pooled s) Lower limit Upper limit CN12 10.0433 1.61057 7.25667 12.83 CN18 0.0 1.61057 -2.78666 2.78666 CN4 29.5333 1.61057 26.7467 32.32 Total 13.1922 Multiple Range Tests for hl nitrate by ty le cn Method: 95.0 percent LSD ty le cn Count Mean Homogeneous Groups Maximum 13.86 0.0 32.5 32.5 P-Value 0.0000 Range 7.63 0.0 5.93 32.5 Stnd skewness 0.00278023 0.00357726 0.569597 CN18 CN12 CN4 3 0.0 10.0433 29.5333 X X X Contrast Sig Difference +/- Limits CN12 - CN18 * 10.0433 5.57333 CN12 - CN4 * -19.49 5.57333 CN18 - CN4 * -29.5333 5.57333 * denotes a statistically significant difference Summary Statistics for hl nitrite B.ty le cn Count Average Standard deviation CN12 54.3867 1.335 CN18 63.14 0.89 CN4 39.16 0.25 Total 52.2289 10.5403 Coeff of variation 2.45465% 1.40957% 0.638407% 20.181% Minimum 53.05 62.25 38.91 38.91 B.ty le cn Stnd kurtosis CN12 CN18 CN4 Total -1.05232 ANOVA Table for hl nitrite by B.ty le cn Source Sum of Squares Df Mean Square F-Ratio Between groups 883.513 441.756 502.60 Within groups 5.27367 0.878944 Total (Corr.) 888.786 Table of Means for hl nitrite by B.ty le cn with 95.0 percent LSD intervals Stnd error B.ty le cn Count Mean (pooled s) Lower limit Upper limit CN12 54.3867 0.541278 53.4501 55.3232 CN18 63.14 0.541278 62.2035 64.0765 CN4 39.16 0.541278 38.2235 40.0965 Total 52.2289 Multiple Range Tests for hl nitrite by B.ty le cn Method: 95.0 percent LSD B.ty le cn Count Mean CN4 39.16 CN12 54.3867 CN18 63.14 Maximum 55.72 64.03 39.41 64.03 Range 2.67 1.78 0.5 25.12 Stnd skewness -0.00794495 0.0 0.0 -0.453706 Range 0.0 10.1695 26.2712 14.41 174.45 Stnd skewness P-Value 0.0000 Homogeneous Groups X X X Contrast Sig Difference +/- Limits CN12 - CN18 * -8.75333 1.87307 CN12 - CN4 * 15.2267 1.87307 CN18 - CN4 * 23.98 1.87307 * denotes a statistically significant difference Khảo sát nồng độ muối (48h) Summary Statistics for hl nitrate nd muoi Count Average Standard deviation NaCl 0% 0.0 0.0 NaCl 3% 14.7034 5.08475 NaCl 5% 119.788 13.1356 NaCl 7% 167.247 7.205 Total 12 75.4345 73.7481 nd muoi Stnd kurtosis NaCl 0% NaCl 3% NaCl 5% NaCl 7% Total -1.446 ANOVA Table for hl nitrate by nd muoi Coeff of variation % 34.5821% 10.9657% 4.30801% 97.7644% Minimum 0.0 9.61864 106.653 160.04 0.0 Maximum 0.0 19.7881 132.924 174.45 174.45 -4.17192E-10 0.0 -0.00147212 0.264978 Source Sum of Squares Df Mean Square F-Ratio Between groups 59326.1 19775.4 316.01 Within groups 500.621 62.5776 Total (Corr.) 59826.7 11 Table of Means for hl nitrate by nd muoi with 95.0 percent LSD intervals Stnd error nd muoi Count Mean (pooled s) Lower limit Upper limit NaCl 0% 0.0 4.56719 -7.44723 7.44723 NaCl 3% 14.7034 4.56719 7.25616 22.1506 NaCl 5% 119.788 4.56719 112.341 127.235 NaCl 7% 167.247 4.56719 159.799 174.694 Total 12 75.4345 P-Value 0.0000 Multiple Range Tests for hl nitrate by nd muoi Method: 95.0 percent LSD nd muoi Count Mean NaCl 0% 0.0 NaCl 3% 14.7034 NaCl 5% 119.788 NaCl 7% 167.247 Homogeneous Groups X X X X Contrast Sig Difference NaCl 0% - NaCl 3% -14.7034 NaCl 0% - NaCl 5% * -119.788 NaCl 0% - NaCl 7% * -167.247 NaCl 3% - NaCl 5% * -105.085 NaCl 3% - NaCl 7% * -152.543 NaCl 5% - NaCl 7% * -47.4585 * denotes a statistically significant difference +/- Limits 14.8945 14.8945 14.8945 14.8945 14.8945 14.8945 Summary Statistics for hl nitrite nd muoi Count Average Standard deviation NaCl 0% 75.25 0.84 NaCl 3% 86.7167 8.255 NaCl 5% 25.7167 0.145029 NaCl 7% 16.2233 0.345012 Total 12 50.9767 32.0159 Coeff of variation 1.11628% 9.51951% 0.563948% 2.12664% 62.805% Minimum 74.41 78.46 25.57 15.88 15.88 nd muoi Stnd kurtosis NaCl 0% NaCl 3% NaCl 5% NaCl 7% Total -1.51634 ANOVA Table for hl nitrite by nd muoi Source Sum of Squares Df Mean Square F-Ratio Between groups 11137.2 3712.4 215.24 Within groups 137.981 17.2477 Total (Corr.) 11275.2 11 Table of Means for hl nitrite by nd muoi with 95.0 percent LSD intervals Stnd error nd muoi Count Mean (pooled s) Lower limit Upper limit NaCl 0% 75.25 2.39775 71.3402 79.1598 NaCl 3% 86.7167 2.39775 82.8069 90.6264 NaCl 5% 25.7167 2.39775 21.8069 29.6264 NaCl 7% 16.2233 2.39775 12.3136 20.1331 Total 12 50.9767 Multiple Range Tests for hl nitrite by nd muoi Method: 95.0 percent LSD nd muoi Count Mean NaCl 7% 16.2233 NaCl 5% 25.7167 NaCl 0% 75.25 NaCl 3% 86.7167 Homogeneous Groups X X X X Maximum 76.09 94.97 25.86 16.57 94.97 P-Value 0.0000 Range 1.68 16.51 0.29 0.69 79.09 Stnd skewness 0.0 -0.00128487 -0.0730959 0.0307398 0.0953026 Contrast Sig Difference +/- Limits NaCl 0% - NaCl 3% * -11.4667 7.81953 NaCl 0% - NaCl 5% * 49.5333 7.81953 NaCl 0% - NaCl 7% * 59.0267 7.81953 NaCl 3% - NaCl 5% * 61.0 7.81953 NaCl 3% - NaCl 7% * 70.4933 7.81953 NaCl 5% - NaCl 7% * 9.49333 7.81953 * denotes a statistically significant difference Summary Statistics for hl amoni nd muoi Count Average Standard deviation Coeff of variation NaCl 0% 14.34 0.61 4.25384% NaCl 3% 7.93333 3.965 49.979% NaCl 5% 6.71333 3.965 59.0616% NaCl 7% 8.85 6.1 68.9266% Total 12 9.45917 4.67326 49.4046% Minimum 13.73 3.97 2.75 2.75 2.75 nd muoi Stnd kurtosis NaCl 0% NaCl 3% NaCl 5% NaCl 7% Total -1.03478 ANOVA Table for hl amoni by nd muoi Source Sum of Squares Df Mean Square F-Ratio Between groups 102.184 34.0614 1.97 Within groups 138.049 17.2561 Total (Corr.) 240.233 11 Table of Means for hl amoni by nd muoi with 95.0 percent LSD intervals Stnd error nd muoi Count Mean (pooled s) Lower limit Upper limit NaCl 0% 14.34 2.39834 10.4293 18.2507 NaCl 3% 7.93333 2.39834 4.02261 11.8441 NaCl 5% 6.71333 2.39834 2.80261 10.6241 NaCl 7% 8.85 2.39834 4.93927 12.7607 Total 12 9.45917 Multiple Range Tests for hl amoni by nd muoi Method: 95.0 percent LSD nd muoi Count Mean NaCl 5% 6.71333 NaCl 3% 7.93333 NaCl 7% 8.85 NaCl 0% 14.34 Maximum 14.95 11.9 10.68 14.95 14.95 Range 1.22 7.93 7.93 12.2 12.2 Stnd skewness 0.0 0.00267505 0.00267505 0.0 -0.381454 Range 0.0 5.93 10.17 0.0 181.23 Stnd skewness P-Value 0.1966 Homogeneous Groups X X X X Contrast Sig Difference NaCl 0% - NaCl 3% 6.40667 NaCl 0% - NaCl 5% 7.62667 NaCl 0% - NaCl 7% 5.49 NaCl 3% - NaCl 5% 1.22 NaCl 3% - NaCl 7% -0.916667 NaCl 5% - NaCl 7% -2.13667 * denotes a statistically significant difference +/- Limits 7.82145 7.82145 7.82145 7.82145 7.82145 7.82145 khảo sát mật độ vi sinh (24h) Summary Statistics for hl nitrate mat Count Average Standard deviation MD10^5 181.23 0.0 MD10^6 154.533 2.965 MD10^7 30.8067 5.085 MD10^8 0.0 0.0 Total 12 91.6425 81.0785 mat MD10^5 MD10^6 Stnd kurtosis Coeff of variation 0.0% 1.91868% 16.5062% % 88.4725% Minimum 181.23 151.57 25.72 0.0 0.0 Maximum 181.23 157.5 35.89 0.0 181.23 0.00357726 -0.00208586 -0.0252418 MD10^7 MD10^8 Total -1.57714 ANOVA Table for hl nitrate by mat Source Sum of Squares Df Mean Square F-Ratio Between groups 72241.6 24080.5 2779.98 Within groups 69.2969 8.66212 Total (Corr.) 72310.9 11 Table of Means for hl nitrate by mat with 95.0 percent LSD intervals Stnd error mat Count Mean (pooled s) Lower limit Upper limit MD10^5 181.23 1.69923 178.459 184.001 MD10^6 154.533 1.69923 151.763 157.304 MD10^7 30.8067 1.69923 28.0359 33.5774 MD10^8 0.0 1.69923 -2.77075 2.77075 Total 12 91.6425 Multiple Range Tests for hl nitrate by mat Method: 95.0 percent LSD mat Count Mean MD10^8 0.0 MD10^7 30.8067 MD10^6 154.533 MD10^5 181.23 Homogeneous Groups X X X X Contrast Sig Difference +/- Limits MD10^5 - MD10^6 * 26.6967 5.5415 MD10^5 - MD10^7 * 150.423 5.5415 MD10^5 - MD10^8 * 181.23 5.5415 MD10^6 - MD10^7 * 123.727 5.5415 MD10^6 - MD10^8 * 154.533 5.5415 MD10^7 - MD10^8 * 30.8067 5.5415 * denotes a statistically significant difference Summary Statistics for hl nitrite mat Count Average Standard deviation Coeff of variation MD10^5 2.0 0.0 0.0% MD10^6 11.0 0.0 0.0% MD10^7 76.6667 0.57735 0.753066% MD10^8 35.0 1.0 2.85714% Total 12 31.1667 30.1958 96.885% mat MD10^5 MD10^6 MD10^7 MD10^8 Total P-Value 0.0000 Minimum 2.0 11.0 76.0 34.0 2.0 Maximum 2.0 11.0 77.0 36.0 77.0 Stnd kurtosis -0.770033 ANOVA Table for hl nitrite by mat Source Sum of Squares Df Mean Square F-Ratio Between groups 10027.0 3342.33 10027.00 Within groups 2.66667 0.333333 Total (Corr.) 10029.7 11 Table of Means for hl nitrite by mat with 95.0 percent LSD intervals Stnd error mat Count Mean (pooled s) Lower limit Upper limit MD10^5 2.0 0.333333 1.45647 2.54353 MD10^6 11.0 0.333333 10.4565 11.5435 MD10^7 76.6667 0.333333 76.1231 77.2102 MD10^8 35.0 0.333333 34.4565 35.5435 Total 12 31.1667 Multiple Range Tests for hl nitrite by mat Method: 95.0 percent LSD mat Count Mean Homogeneous Groups P-Value 0.0000 Range 0.0 0.0 1.0 2.0 75.0 Stnd skewness -1.22474 0.0 1.03011 MD10^5 MD10^6 MD10^8 MD10^7 3 3 2.0 11.0 35.0 76.6667 X X X X Contrast Sig Difference MD10^5 - MD10^6 * -9.0 MD10^5 - MD10^7 * -74.6667 MD10^5 - MD10^8 * -33.0 MD10^6 - MD10^7 * -65.6667 MD10^6 - MD10^8 * -24.0 MD10^7 - MD10^8 * 41.6667 * denotes a statistically significant difference +/- Limits 1.08706 1.08706 1.08706 1.08706 1.08706 1.08706 ... để xử lý nước thải giàu nitơ BỘ GIÁO DỤC VÀ ĐÀO TẠO TRƯỜNG ĐẠI HỌC KỸ THUẬT CÔNG NGHỆ TP HCM - HUỲNH VĂN THÀNH PHÂN LẬP TUYỂN CHỌN VI SINH VẬT XỬ LÝ LOẠI BỎ NITƠ TRONG NƯỚC THẢI CHẾ... danh vi sinh vật (hình thái, sinh lý, sinh hóa, phân tích gene) PP định lượng vi sinh vật PP phân tích hóa học, N-NH4+, N-NO3, N-NO2-…(hóa nước) 3 KẾT QUẢ THẢO LUẬN Phần Tăng sinh, phân lập khảo... nhất, phân lập từ nước thải chợ đầu mối thủy sản Sàng lọc – Loại bỏ vi khuẩn gây bệnh qua test sinh hóa ? ?Nước thải CBTS chứa VK đường ruột, Vibrio spp Các VK khử nitrate, khử nitrite sinh NH4+