Giải trình tự hệ gen lục lạp của sâm ngọc linh (panax vietnamensis ha et grushv)

ĐẠI HỌC QUỐC GIA HÀ NỘI TRƢỜNG ĐẠI HỌC KHOA HỌC TỰ NHIÊN Nguyễn Nhật Linh GIẢI TRÌNH TỰ HỆ GEN LỤC LẠP CỦA SÂM NGỌC LINH (Panax vietnamensis Ha et Grushv.) LUẬN VĂN THẠC SĨ KHOA HỌC Hà Nội – 2017 ĐẠI HỌC QUỐC GIA HÀ NỘI TRƢỜNG ĐẠI HỌC KHOA HỌC TỰ NHIÊN Nguyễn Nhật Linh GIẢI TRÌNH TỰ HỆ GEN LỤC LẠP CỦA SÂM NGỌC LINH (Panax vietnamensis Ha et Grushv.) Chuyên ngành: Mã số: Sinh học thực nghiệm 60420114 LUẬN VĂN THẠC SĨ KHOA HỌC NGƯỜI HƯỚNG DẪN KHOA HỌC : TS Lê Thị Thu Hiền PGS TS Võ Thị Thương Lan Đại diện cán hướng dẫn: Lê Thị Thu Hiền Hà Nội – 2017 LỜI CẢM ƠN Để thực thành công luận văn thạc sĩ này, xin gửi lời cảm ơn sâu sắc chân thành đến TS Lê Thị Thu Hiền (Viện Nghiên cứu hệ gen, Viện Hàn lâm Khoa học Công nghệ Việt Nam), người trực tiếp hướng dẫn, bảo tận tình suốt thời gian tơi thực đề tài Cô không giúp đỡ vượt qua khó khăn, truyền thụ cho tơi kiến thức, kinh nghiệm chuyên môn quý báu công việc mà cịn giúp tơi phát triển khả tư duy, định hướng nghiên cứu tác phong làm việc khoa học Điều giúp thêm tự tin đường nghiên cứu khoa học sau Trong suốt thời gian học tập nghiên cứu vừa qua, tơi nhận dạy dỗ, khích lệ hỗ trợ nhiều mặt PGS TS Võ Thị Thương Lan (Khoa Sinh học, Trường Đại học Khoa học tự nhiên, Đại học Quốc gia Hà Nội) Sự quan tâm, giúp đỡ góp phần khơng nhỏ giúp tơi hồn thành luận văn tốt nghiệp Tơi xin chân thành cảm ơn trợ giúp quý báu Tơi xin bày tỏ lịng biết ơn đến PGS TS Nơng Văn Hải (Chủ tịch Hội đồng Khoa học, Viện Nghiên cứu hệ gen), tập thể cán Phòng Đa dạng sinh học hệ gen cán Viện Nghiên cứu hệ gen, Viện Hàn lâm Khoa học Công nghệ Việt Nam tạo điều kiện thuận lợi tận tình hướng dẫn, trợ giúp thực tốt đề tài nghiên cứu Tôi xin chân thành cảm ơn thầy cô thuộc Khoa Sinh học, Trường Đại học Khoa học tự nhiên, Đại học Quốc gia Hà Nội thầy cán Phịng thí nghiệm Sinh Y, dạy dỗ dìu dắt tơi bước đường nghiên cứu khoa học Luận văn thực khuôn khổ đề tài cấp Viện Hàn lâm Khoa học Công nghệ Việt Nam: “Giải mã hệ gen lục lạp sâm Ngọc Linh (Panax vietnamensis Ha et Grushv.)” Hà Nội, ngày 05 tháng 12 năm 2017 Học viên cao học Nguyễn Nhật Linh MỤC LỤC MỞ ĐẦU CHƢƠNG 1: TỔNG QUAN TÀI LIỆU 1.1.Chi Nhân sâm sâm Ngọc Linh 1.2.Các cơng nghệ giải trình tự DNA 1.2.1 Các hệ thống giải trình tự DNA hệ 1.2.2 Các hệ thống giải trình tự DNA hệ HT-NGS (high-throughput next generation sequencing) 1.3.Giải trình tự gen hệ gen lồi thuộ 1.3.1 Giải trình tự gen phục vụ định loại phân tử 1.3.2 Giải trình tự hệ gen nhân hệ gen biểu 1.3.3 Giải trình tự hệ gen lục lạp 1.4.Phân tích phát sinh chủng loại CHƢƠNG 2: VẬT LIỆU VÀ PHƢƠNG PHÁP 2.1 Vật liệu 2.1.1 Vật liệu thực 2.1.2 Hóa chất, dụn 2.2 Phương pháp 2.2.1 Sơ đồ thí ngh 2.2.2 Tách chiết 2.2.3 Phân tách 2.2.4 Tạo thư viện 2.2.5 Giải trình tự 2.2.6 Lắp ráp trình 2.2.7 chỉnh Khuếch đại 2.2.8 Phân tích 2.2.9 Xây dựng 2.2.10 Tìm kiếm, phân tích đánh giá vùng trình tự mã vạch phân tử tiềm i CHƢƠNG 3: KẾT QUẢ VÀ THẢO LUẬN 33 3.1 Kết tách chiết, kiểm tra chất lượng DNA tổng số mẫu sâm Ngọc Linh phân tách phân đoạn DNA bị methyl hóa, khơng bị methyl hóa 33 3.1.1 Tách chiết kiểm tra chất lượng DNA tổng số mẫu sâm Ngọc Linh 33 3.1.2 Phân tách phân đoạn DNA bị methyl hóa khơng bị methyl hóa CpG 34 3.2 Giải trình tự hệ gen lục lạp sâm Ngọc Linh 36 3.3 Kết lắp ráp gen hệ gen lục lạp sâm Ngọc Linh 38 3.4 Kết hiệu chỉnh/ kiểm chứng trình tự hệ gen lục lạp sâm Ngọc Linh 39 3.5 Kết phân tích giải hệ gen lục lạp sâm Ngọc Linh .41 3.6 Đề xuất thị phân tử tiềm khai thác từ trình tự hệ gen lục lạp sâm Ngọc Linh 45 KẾT LUẬN 49 KIẾN NGHỊ 49 DANH MỤC CÁC CÔNG TRÌNH CƠNG BỐ 50 LIÊN QUAN ĐẾN LUẬN VĂN 50 TÀI LIỆU THAM KHẢO 51 ii DANH MỤC CÁC KÝ HIỆU, CHỮ VIẾT TẮT bp DNA dNTPs ENA EST IR kb LSC ML mPTP NCBI NGS PCR RNA-Seq SNP SSC TE v/p WGS iii Bảng 1.1: Bảng 1.2: Bảng 3.1: Bảng 3.2 chọn iv DANH MỤC CÁC HÌNH Hình 1.1: Hình 1.2: Hình 1.3: Hình 1.4: Hình 2.1: Hình 2.2: Hình 3.1: Hình 3.2: Hình 3.3: Hình 3.4: Hình 3.5: Hình 3.6: Hình 3.7: Hình 3.8: Hình 3.9: Hình 3.10: v MỞ ĐẦU Sâm Ngọc Linh (Panax vietnamensis Ha et Grushv.) thuộc chi Nhân sâm (Panax) cịn có tên gọi khác sâm Việt Nam, sâm Khu Năm (K5), sâm trúc (sâm đốt trúc, trúc tiết sâm), củ Ngải rọm hay Thuốc giấu, lồi đặc biệt có giá trị khoa học kinh tế với thành phần saponin, hàm lượng acid amin, chất khoáng vi lượng củ, rễ nhiều loài sâm khác Ngoài tác dụng dược lý, sâm Ngọc Linh cịn giúp chống căng thẳng, trầm cảm, oxy hóa Do vùng phân bố hạn chế việc khai thác mức khiến sâm Ngọc Linh trở nên khan tự nhiên đưa vào Danh lục đỏ IUCN (2003) danh sách loài hạn chế khai thác sử dụng mục đích thương mại Trên giới Việt Nam, nghiên cứu định loại phương pháp hình thái kết hợp với phân tử thơng qua phân tích số vùng gen loài thuộc chi Nhân sâm, có sâm Ngọc Linh thực Nhờ đời phát triển hệ thống giải trình tự gen hệ mới, trình tự tồn hệ gen, có hệ gen biểu hiện, hệ gen lục lạp số loài thuộc chi Nhân sâm triển khai thực năm gần Việc phân tích khai thác sở liệu toàn hệ gen cung cấp nguồn thơng tin lớn, có giá trị độ tin cậy cao, hỗ trợ nghiên cứu phát sinh chủng loại, q trình thích nghi, nhận dạng lồi phục vụ giám sát thương mại bảo tồn nguồn gen Nhằm đáp ứng nhu cầu ngày tăng nghiên cứu tiến hóa, bảo tồn, khai thác sử dụng bền vững nguồn gen sâm Ngọc Linh quý góp phần tìm kiếm mã vạch phân tử đặc trưng định hướng ứng dụng giám định chất lượng sâm Ngọc Linh loài thuộc chi Nhân sâm Việt Nam, đề tài: “Giải trình tự hệ gen lục lạp sâm Ngọc Linh (Panax vietnamensis Ha et Grushv.)” xây dựng thực với nội dung sau: (1) Tách chiết DNA giải trình tự hệ gen lục lạp sâm Ngọc Linh; (2) Lắp ráp hiệu chỉnh trình tự hệ gen lục lạp; (3) Chú giải phân tích trình tự hệ gen lục lạp; (4) Phân tích tìm kiếm thị phân tử thích hợp để phân biệt sâm Ngọc Linh với loài thuộc chi Nhân sâm TAGGGGTGCAGAGACAGCCAGGAGGTTTGCCTAGAAGCAGCCACCCTTGAAAGAGTGCGTAATAGCTCA CTGATCGAGCGCTCTTGCGCCGAAGATGAACGGGGCTAAGCGATCTGCCGAAGCTGTGGGATGTAAAAA TACATCGGTAGGGGAGCGTTCCGCCTTAGAGGGAAGTACCCGCGCGAGCGGTAGTGGACGAAGCGGAA GCGAGAATGTCGGCTTGAGTAACGCAAACATTGGTGAGAATCCAATGCCCCGAAAACCTAAGGGTTCCT CCGCAAGGTTCGTCCACGGAGGGTGAGTCAGGGCCTAAGATCAGGCCGAAAGGCGTAGTCGATGGACA ACAGGTGAATATTCCTGTACTACCCCTTGTTGGTCCCGAGGGACGGAGGAGGCTAGGTTAGCCGAAAGA TGGTTATCGGTTCAAGGACGTAAGGTGACCCTGCTTTTTCAGGGTAAGAAGGGGTAGAGAAAATGCCTC GAGCCAATGTTCGAGTACCAGGCGCTACGGCGCTGAAGTAACCCACGCCATACTCCCAGGAAAAGCTCG AACGACCTTCAACAAAAGGGTACCTGTACCCGAAACCGACACAGGTAGGTAGGTAGAGAATACCTAGGG GCGCGAGACAACTCTCTCTAAGGAACTCGGCAAAATAGCCCCGTAACTTCGGGAGAAGGGGTGCCTCCT CACAAAGGGGGTCGCAGTGACCAGGCCCGGGCGACTGTTTACCAAAAACACAGGTCTCCGCAAAGTCGT AAGACCATGTATGGGGGCTGACGCCTGCCCAGTGCCGGAAGGTCAAGGAAGTTGGTGACCTGATGACA GGGGAGCCGGCGACCGAAGCCCCGGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATT CCTTGTCGGGTAAGTTCCGACCCGCACGAAAGGCGTAACGATCTGGGCACTGTCTCGGAGAGAGGCTC GGTGAAATAGACATGTCTGTGAAGATGCGGACTACCTGCACCTGGACAGAAAGACCCTATGAAGCTTCAC TGTTCCCTGGGATTGGCTTTGGGCCTTTCCTGCGCAGCTTAGGTGGAGGGCGAAGAAGGCCTCCTTCCG GGGGGGCCCGAGCCATCAGTGAGATACCACTCTGGAAGAGCTAGAATTCTAACCTTGTGTCAGGACCTA CGGGCCAAGGGACAGTCTCAGGTAGACAGTTTCTATGGGGCGTAGGCCTCCCAAAAGGTAACGGAGGC GTGCAAAGGTTTCCTCGGGCCGGACGGAGATTGGCCCTCGAGTGCAAAGGCAGAAGGGAGCTTGACTG CAAGACCCACCCGTCGAGCAGGGACGAAAGTCGGCCTTAGTGATCCGACGGTGCCGAGTGGAAGGGCC GTCGCTCAACGGATAAAAGTTACTCTAGGGATAACAGGCTGATCTTCCCCAAGAGCTCACATCGACGGGA AGGTTTGGCACCTCGATGTCGGCTCTTCGCCACCTGGGGCTGTAGTATGTTCCAAGGGTTGGGCTGTTC GCCCATTAAAGCGGTACGTGAGCTGGGTTCAGAACGTCGTGAGACAGTTCGGTCCATATCCGGTGTGGG CGTTAGAGCATTGAGAGGACCTTTCCCTAGTACGAGAGGACCGGGAAGGACGCACCTCTGGTGTACCAG TTATCGTGCCCACGGTAAACGCTGGGTAGCCAAGTGCGGAGCGGATAACTGCTGAAAGCATCTAAGTAG TAAGCCCACCCCAAGATGAGTGCTCTCCTATTCCGACTTCCCCAGAGCCTCCGGTAGCACAGCCGAGAC AGCGACGGGTTCTCTGCCCCTGCGGGGATGGAGCGACAGAAGTTTTGAGAATTCAAGAGAAGGTCACG GCGAGACGAGCCGTTTATCATTACGATAGGTGTCAAGTGGAAGTGCAGTGATGTATGCAGCTGAGGCAT CCTAACAGACCGATAGACTTGAACCTTGTTCCTACATGACCCGATCAATTCGATCAGGCACTCGCCATCT ATTTTCATTGTTCAACTCTTTGACAACACGAAAAAACCCTTGTTCAACAAAAAACCAAAAGCTCTGCCCTCC CTCTCTATCTATCCAAGGGATGGAAGGGCAGAGGCCTTTGGTGTCCCCTCCAGTCAAGAATTGGGGCCT CACAATCACTAGCCAATATGCTTTTCTCTCATGCCTTTCTTCGTTCATGGTTCGATATTCTGGTGTCCTAG GCGTAGAGGAACCACACCAATCCATCCCGAACTTGGTGGTTAAACTCTACTGCGGTGACGATACTGTAG GGGAGGTCCTGCGGAAAAATAGCTCGACGCCAGGATGATAAAAAGCTTAACACCTCCCATTCTTATTTAT TTTTCAATATGAAAAATAAATAAATGAAAAATCAATCAAAAGGTCGTCTTATTCAAAACCCCAATTATGACAT CCCCTCTCTCCCACTTCACACCTCGGAACGCACCGTTCCGTTCGTATAGAGAGAAAGGCGCTTTCACATC TTCTTAACCCGAAATGGCTGGGGAGAGGAAAGGTTCCTTTTTTTGAGGGTACTCCCGGGAACAGATCCAG TGGAGACGGGGTGGGGCCTGTAGCTCAGAGGATTAGAGCACGTGGCTACGAACCACGGTGTCGGGGGT TCGAATCCCTCCTCGCCCACAACCGGCCCAAAAGGGAAGGGCCTTTCCCTCTGGGGGTAGGAAAATCAT GATCGGGATAGCGGACCAAAAGCTATGGAACTTGGGTGTGGGTCTTTTGTCGAAATGGAATGGCCCTTTT TTTTTATTTTATTTATCGTAAATGGTTTATTCATTACACATAGTATGCCCGACCCCCCTCAGCGTATTTTTTG TTTTACGCCCCGTAACTCTTCCTCAGCCAGGCTGGGGCAGAATAGCAGAGCAAGTACAAGTATTAGTAGC ATAACAAAAATGCGTTCCTCGTCATTAATATGTTTGCTCACGGCAATTGTGGCCTCTCGGGAGAATCGATG ACTGCATCTTTGATGCACTGCTAGTACTAGTACATCTGAGAATTCTTAATTGGATAGTTGTAAATAGCCCC AGGGCTATGGAACAAAGGATTATCCCGGACCTACACCGAGGTATTGACGGTGATTTTAAAATCTCGCAGA ACAGGATGTGATACGATGAGATAGAATGCAATAGAAACAAAGACGGGGAATGGGTTACCTACTCTTAACG GTCAAAGCGAGCCCTTTTATTCTGAATTAAAGAATTCAGAATGAATCAAATCTCCCCAGGTAGGATTCGAA CCTACGACCAGTCAGTTAACAGCCGACCGCTCTACCACTGAGCTACTGAGGAACAACGAGAGATTAGAT CTCATAGAGTTCAATTCCCGTTCTCAACCCATGACCAATATGAGCTCGAAGCTTCCTTCGTAACTCCCGGA ACTTCTTCGTAGTGGCCCCCTTCCATGCCTCATTTCATAGGGAACCTCAAAGTGGCTCTATTTCATTATAT TCCATCCATATCCCAATTCCATTCATTTAATATCCCTTTGGTGTCATTGACATAACAGATGTCGTTTCTAGT CTATCTCTTTCTATTTCTTTTCTATATATGGAAAGTTCAAAAATCATCATATAATAATCCAGAAATTGCAATA GAAAAGAAAAAGGGAGGTTTGTGATGATTTTGAAATCTTTTCTACTAGGTAATCTAGTATCCTTATGCATGA AGATAATCAATTCGGTCGTTGTGGTCGGACTCTATTATGGATTTCTGACCACATTCTCCATAGGGCCCTCT TATCTCTTCCTTCTCCGAGCTCGGGTTATGGAAGAAGGAGAAGAAGGAACCGAGAAGAAGGTATCAGCA ACAACAGGTTTTATTACGGGACAGCTCATGATGTTCATATCGATCTATTATGCGCCTCTGCATCTAGCATT GGGTAGACCTCATACAATAACTGTACTAGCTCTACCGTATCTTTTGTTTCATTTCTTCTGGAATAATCACAA AAACTTTTTTGATTATGGATCTACTACCAGAAATTCAATGCGTAATCTTAGCATTCAATGCGTATTCCTGAA 90 TAATCTCATTTTTCAATTATTCAACCATTTCATTTTACCAAGTTCAATGTTAGCCAGATTAGTCAACATTTAT ATGTTTCGATGCAACAACAAGATGTTATTTGTAACAAGCAGTTTTGTTGGTTGGTTAATTGGTCACATTTTA TTCATGAAATGGGTTGGATTGGTATTAGTCTGGATACGGCAAAATAATTCTATTAGATCGAATGTACTTATT CGATCTAATAAGTACCTTGTGTCAGAATTGATAAATTCTATGGCTCGAATCTTTAGTATTCTCTTATTTATTA CCTGTGTCTACTATTTAGGCAGAATACCGTCACCCATTATTACTAAGAAACTGAAAGAAACCCCAACAACG GAAGAAAGGGGGGAAAGTGAGGAAGAAACCCCAACAAAGGAAGAAAGGGGGGAAAGTGAGGAAGAAAC AGATGTTGAAATAGAAACAGCTTCCGAAACGAAGGGGGCTAAACAGGAACAAGAGGGATCCACCGAAGA AGATCCTTCTCCTTCCCTTTTTTCGGAAGAAAAGGAGGATCCGGACAAAATCGATGAAACGGAAGAGATC CGAGTGAATGGAAAGGAAAAAACAAAGGATGAATTCCACTTTACAGAGACATCCTATAACAATAGCCCAG TTTATAAAGTTTCTTATTTGTATAAGAATCAAGAAAATTTTAACAAAATAAAAATACTTGATAAAAAAGATGA AGATAAAGGCCTCTTCTGGTTTGAAAAACCTCTTGTGACCCTTCTTTTCGATTATAAACGATGGAATCGCC CATTACGATACATAAAAAATAATAGATTTGAAAGGACTGCAAGAAATGAAATGTCACAATATTTTTTTTATA CATGCCGAAGTGATGGAAAAGAAAGAATATCTTTTACGTATCCGCCTAGCTTGTCAACTTTTGGAGAAATG ATACAAAGAAGGATGTCCTTGCCCACACTAGAAAAACTCTCTTCGGATGAATTGTACAATCATTGGATTTA TACTAACAAACAAAAAAAGAACAACTTAAACAACGAGTTTTTAAATAGAATTAAGGCTCTAGATATGGGATT TCTTTCTCTAGATATACTCGAAAAAAGTACTAGATTGTATAATGACACTAAAAAAGATTACTTGCCTAAAAA GTAGTAAATTAATAAAAAGATTGATACATAAGACAAATACAAGAAAAGATAAGAAGAGATGCGCCCCCCAC CTACAGATTTGATACTTTCGCCTACAAAAAAACTCAAAACACCAACTCCATTTGTAATTCCATCAATTACTT GTCTATCAAAAAAAGAAGTCAATTCGGCCAATCCTCTTATCCCCCCAGTAAAGAATGTTGCATAAAAAGCA TCTATGTAACCACGATTACACGACCAACCATATATACCATTTATTATTTTGTCCAAAAAAATTCTCTTAGGA CCTATTTTAACAAACGAGTTAATTAAGTCCAAATTTTGCAAAGAGGAATAAACGGGTTTATATAAAAAGGAG GCTATAAAGATTCCAAAAAAAGCTATACTAACTGAAAAGATGGCATCTTTCGCAAATTCATCCCAATCTATT AAATTATTTAAATTTTGATGTAAAAGGTTTATAGACGGGGTTAACCATTTTGATAATATGTCCAAATTCGCT CCTTCTTGATTGAAAGGAATTCCTAGGAATCCAATGAACAAAGTGAATAGAACCAATACAAGTATAGGAAA TAACATAGTATTATCTGATTCATAAGGATATGAAAAAATATTCTTATTGCCAAAATAGGTAATAGTAATAAAG GGTTGTGTGATGTTTCTTACATTCTGATCCATTTGATATGTCTTCTTTGAAAAAAAAGAAGCAATTTCTTGA TTATTCTTGTTATTCATTGTTAATAAAGGAAAATTTTTATTAATCCTTTTCGAACCTTCTTTACCCCATAGAG ATATTGAATAGAAGGGGGTATTTTTGTAATTTTGAAAATGAACGTTTAAATGTCCTTCAAAAGTAAGTAAAT AGATTCGAAACATATAAAATGCGGTTAATCCCGCTGTAGACCAAGCTATTATTGCAAAAATTGGTGAATAC AACCAAGTATCATTAATAATTTCATCCTTGGACCAAAAACAAGCAAGAGGCGGAATACCACAAAGAGAAAG TGTACCGAATAAAAAAGACGTTTTGGTAATTGGCACATGCTTTGTTAAACCCCCCATAAAAACCATATTCT GGCTTTTCTTTGGAGAATATCCAACAAGAGTTTCCATTGAATGAATAACGGATCCGGATCCTAAAAACAAT AATGCTTTCGAATAAGCATGAGTAATCAAATGAAATAAAGCACTTCGATAAGACCCCATACCTAAAGCTAA CATCATATAACCCAATTGAGACATTGTGGAATAGGCCAAACCTCTCTTAATGTCTTTTTGAGCAAGGGCTA AAGTAGCTCCTAATAATACTGTTATTATCCCTACCAAAGAGATTAAATTCATTATGTGAGGGATGACTATGA AAAGAGGAATAAGCCGAGCTACAAGAAAAATTCCCGCTGCTACCATAGTAGCAGCATGTATAAGAGCTGA AATAGGAGTAGGCCCCTCCATGGCATCAGGTAACCATACATGAAGGGGGAATTGTGCAGATTTAGCAAC CGCACCGGCAAATAATAGAACGGCACACAAAGTAACAAATAAAAAATTCACTTCGTTATTATAAATCAAGT TACTGAATATTTTGAATAAATCTCGAAATTCGAAACTACCTGTTATCCAATAAAAACCTAAAATTCCTAATAA TAAACCAAAATCCCCGACACGATTAGTTACAAACGCCTTTTGGCAAGCATTTGCCGCAACAGGTCGTGTG AACCAAAACCCTATTAATAGATACGAACACATTCCGACTAATTCCCAAAAAATATAAATTTGTATCAAATTA GAACTAGTAACTAATCCTAACATAGAAGTACTGAAAAAACTCATATAAGCGAAAAATCTCAAATATCCTTGA TCATGAGACATATAATTATCACTATAAACAAGAACCACAATCCCAACAGTAGTAATTAATATTGACATAATA GAAGTAAGTGGGTCGATCAAGTAGCCGAATTCTAAAGAAAAATCATTATTGATGATCCAAGACCACACATA TTGATAGATAGAACTGCTATTTATTTGCTGAATAGACAGATTGATCGAAAAAATCATGACTATACTTAACAA TAAAACACTCTGAAAAGCCCACATACGACGAAGATTTTTTGTTGCCGTCGGAAAAAGAAGAAGTCCCACT CCTATTAACATAGGAACTGGAAGTGGAAGGAAAGGTATTATCCACGCATATTGATATGTCTGTTCCATAAA AAAGTTTTTTATTCTTAATTAATTGTTTCCGATTTACCGGATCTTACCTCTTTCAAAAGGAGTCAATAAAAAA ATCAAGATATGCACTAACTTAAAGAGAATTTTCTACTCTTACTTATTCTGAGTCTTTCGAAAATACTTCAAAT ATTCAAATCAAGAAGTTAGAATTAATTGGTCAAATGAGATGACCCACCTGTTCGTAAAAAGCGAATACTTA GTTATTAACTAAGATTTTTATGAAGATACCGAAAATAAAAATTTCAATTATTATTACATGAATTTATATTCATA GAATAAGGATAAAGAATTACTCTAAAAAGAGTACTTAATTCTGCTATGAATCATAGGGTTAACTAAGATAAA AAACTTGTCCTAATAAAATAAGAACTTTTAGTTAGTTTGTTCCAAATCATTAACTAGTTCATTATGGAATTGA TTTATTATTTTCCATTTCAATAAAAAAAACATTTATAGATTATAGAAAACGCTATAATATCCGACATTTTATAT AAAATTAACTTGATTTTCTATTTATCGATATTTATCGAAAGGGGTCAAATAAAATTAATAAAAAAAAACTAAG ATCTCTTTTATCAAACCACGTATCCTTTAACAGATTAATAACTTTAATTACTAGTCTCATTCAAATTTTAAAAT GAAATTTAGGAGTATTCTTTCCTCGAATTTGACCGGTTAGTAGGAAAAGATTAAAGTTTTCATTAGGCAAT GGGTTCTTAAACCCCTTGGTATATTTTATTCCTTATAAATTAATTAAATGTAGAACGAAGAAAATAGACAGA 91 GATAAAAAGGAAGGTCTTTTTTTGGATTCTTTCTTTTATTAAGTTCAACTAATTAGATTTCTGTGGCATAAC GTCGGATTCTATAGATATAAATGTGAATCATAAAACAAGAAATAAAGGTACCAATCAATAAAAATGAGTCTT TCTTACGAATATTGTATGTATATAAAAAAACTCTTTTTGTTGAAAAAATCACATATCATTTTCTTTTTCTAGTC ATATTTTATACGATTTTCATAGATCTCTATTTTTTTTGTTTTATGAAGAGAATATTATCTAGAGAAGAAATAG ATAATGAATAGTAAAGAACGATTCATTTTGACTAAAAGATGTCTTTCACATCCAACTATAACAACGGGTAAC CTCTTTATTTTAAAATGGCAGTTCCAAAAAAACGTACTTCTATATCAAAAAAGCGTATTCGTAAAAATATTTG GAAAAAAAAGGGATATTGGGCAGCCTTAAAAGCGTTGTCATTAGGGAAATCTCTTTCTACTGGAAATTCAA AAGGTTTTTTTGTGCGACAAACAAATAAGTCATAAAATAAAACATTGTAATAATATGAATCGAAAAATTGGC TTATTTCGCCGTTAATATGAAATTAAAAATTTCCATTACCTATTGTTTGAGGTTAATCAATATGAAATGGAAC TCGCTTCGATCTTAGGATATGTAAACTAAGAATTCTTCAGTTTACTAAATTCTAAAAAAACTTTTGAAAAAA GACAAGATGCAAAGTTTGAACTTTATTTTTAGTCTTTTTTTTTTCATTTTGGGGTGGGGAGTCTTTTTTCCC CATCAACTTATTTATCCCAATTACAAAAATGAAAGTTTTTCTTTTTTCTCTATATCTATAGAAGGGCAAATAT AATATCTTTAGTTAAAATTAATGAATCGTTGAAACTAATTGATTCTAGTTATTTTGAAAACTTTTTGTGTAAC TTTATAACTTCTTATTTCTCGGAATTCATATAATTAATATATCAATCTCCCACATATCTCCCGTTTTCAACCC AATCAACAATGGAATATTTTGTCCAAATAATGTGTCAGTTTTGAGTAATCAAAAATAGGGCCTATTTTGCGT TCATTGATAAATAAAATACATTTTTTAGTTCTATCACTAACCAGAGGGTCGAACTTTCTAATTACAAGAATTC GATTCGAAAAGAGCATAAACTATAACAAAGCCCTAAAGTAAATAAAACCGACACCCCAAAAAAATGAACCT TAAAATTCCTATTTGAATGAAGTTTTTTCATCCATTTGAATCTTTACTAAAAATATTTCATTGAATTAACTCCT TCAATCTCGACGATTGACTATGAATAGGCTATTATGAGTTCGAGCAAGCCGCTATGGTGAAATCGGTAGA CACGCTGCTCTTAGGAAGCAGTGCTAGAGCATCTCGGTTCGAGTCCGAGTGGCGGCAGACCATCTTGTA AAAGAGCTACAATATAATGAATTCCATTTCTGATTTATATTTCAGGATTCCTCCTTTTTAACATTTTTTATGA TATTTTCAACTTTAGAGCATATATTAACTCATATTTCCTTTTCAATCGTTTCAATTGTAATTACAATTCATTTG ATAACCTTATTAGTCGATGAAATCATAAAACTATATGATTCGTCAGAAAAGGGAATGATAGCTATTTTTTTA TGTATAACAGGATTATTAGTCACTCGTTGGATTTATTCGAGACATTTTCCACTAAGTGATTTATATGAATCA TTAATCTTTCTTTCATGGAGTTTATCAGTTATTCATATAGTTCCGTATTTCAAAAAAAAGAAAAAAAATTTAA GCACAATAACTGCGTCAAGTGTTATTTTTACCCAGGGCTTTGCTACTTCAGGTCTTTTAACTGAAATACAT CAATCCGCAATATTAGTACCCGCTCTCCAATCCGAGTGGTTAATAATGCACGTAAGTATGATGATATTGAG CTATGCAGCTCTTCTATGTGGATCATTATTATCAGTAGCACTTCTAGTCATTACATTTCGAAAAAACAGAAA TATTTTTTGTAAAAGGAATCCTTTATTAAATGAGTTATTTTCCTTTGGTGAAATCCAATACATGAATGAAAGA AACAATGTTTTAAGAACTACTTTTTTTTCTGCTAAGAATTATTACAGGTCCCAGTTGATTCAACAGTTGGAT TATTGGAGTTATCGTGTCATTAGTCTAGGGTTTATCTTTTTAACCATAGGTATTCTTTCAGGAGCAGTATGG GCTAATGAAGCATGGGGATCATATTGGAATTGGGACCCAAAGGAAACTTGGGCATTTATTACTTGGATCG TATTTGCGATTTATTTACATACTCGAACCAATATAAAATTACGGGGTGCAAATTCCGCAATTGTGGCGTCT ATAGGCTTTCTTATAATTTGGATATGCTATTTCGGAGTCAATCTATTAGGAATAGGACTACATAGTTATGGT TCATTTACGTTAACGTCTAATTGAATTCAAAAAAGGTTCTTACGACTATAAAAATATAAATAGAATACGGTG TAACTTTATGTGAACCGACGAGAGCCATGTGAATCAAGTAGTGTGGGGATTCACACGGTTCTCACAAAGA CCAAACATATCGGGTTAAAATTCATTTTTTAACTTAAGCTTAAGAGAGAAAAAAGTCTTCTTTTTTTCATTAT CCAAATCCTAGGATTTGGATAATGAAAAACTATCTATAAAAAAAATTAGATAGAATAACTTCGACCTTGTCA ACTGATAGTGAAAGAACAAAATCCGGGTACATACCAATACCTATTACGGGTAGAAAGATAGAGATCGAAA CAAATAACTCTCTTGGTCCAGAATCAAAAACATAAGAGTTTGGAGCATTAAATAGCTTGTATCCATAGAAC ATCTGGCGTAACATAGATAATGAATAAATAGGAGTTAATATCATCCCAATTGCCATTACAAAAGTAATTAAG ATTTTTGGCATTAAAAGATATTTTTGGCTGGTAATTATTCCAAAAAAGACTATCAATTCTGCAACAAAACCA CTCATACCCGGTAATGCAAGGGAAGCCATCGAAAAGCTAGTGAACATCGTGAATATTTTTGGCATTGGGA TAGCTATTCCGCCCATTTCGTCGAGATAAATAAGACGTATTCTATCATAACTCGTTCCTGCCAAGAAAAAA AGCGCAGCACCGATAAATCCATGAGAGATTATTTGTAAAATAGCTCCATTGAGTCCCGTATCGGTTATAGA ACCAATTCCTATAAGTATGAAACCCATATGAGATACAGAGGAATAGGCTATTCTTTTTTTTAAATTACGTTG GCCGGGAGATGTTGAAGCTGCATAGATTATTTGTATTGTGCCTACTATCACCAACCACGGAGAAAATATA GAATGGGCGTGGGGTAATAATTCCATATTAATCCGAATCAATCCATAGGCTCCCATTTTTAGTAAAATTCC GGCTAGAAGCATACAAGTACTGTAATGTGCTTCTCCGTGGGTATCTGGTAACCATGTATGTAGGGGTATA ATCGGCGATTTGACAGCAAAAGCAATAAAAAATCCAATATAGAATATTATTTCCAAGGCCACAGGATATGA CTGATTAACTGATGTTTCAAAATTTAATGTTGGTTCATTAGAACCATATAAACCAACACCCAGAACTCCCAT TAATAGAAAAATGGAACCTCCCGCCGTGTATAAAATAAATTTTGTAGCCGAGTAAAGTCGTCTCTTTCCCC CCCATATGGATAAAAGTAGATAAACCGGAATTAATTCGAACTCCCACATGATGAAAAAAAGTAAAAGGTCC CGAGAAGAAAACGATCCTATTTGCCCGCTGTACATTGCTAACATCAGGAAATGGAATAATCGAGGATCTC GAGTAACAGGCCGAGCCGCTAAAGTAGCTAAAGTGGTGATGAATCCCGTTAGTAAAATGGGTCCTATAGA AAGTCCATCTATTCCTAATCTCCAATGGAAAGCAAAAAAATTGATCCATTTATAGTCCTCCACCAGTTGGAT TAATGGATCGTCGAATTGAAAATGATAACAGAATGCATAGGTCGTTAGAAGGAGTTCTAAAAGACATATAC ATATAGTATACCACCGAATTACTCTATTTCCTCTATGGGGAAGAAAGAAAATTACGGAGCCCGCGAATATT 92 GGCAAAACTACAATTATTGTTAACCAAGGAAAAAAATTCGTGGTAAAGACAAGATACGCTTGGACCAGAAA AACCCGCGCTCAAAATAACATATTTTGAGCACGGGTTTTGTCGGTAAAAAAAATCAAATGGATTCAAATAG AGTTCTTTGGAACATATCAATAACCTAGACCCATGCTGCGAGTTGTTTCATGCCATAAAGAAACTCGAACA CTCAAGAAATCGGTTGGACAGGCGGATTCACATCTCTTACAACCGACACAGTCCTCTGTTCTTGGAGCAG AAGCAATTTGTTTAGCTTTACATCCGTCCCAAGGTATCATTTCTAATACATCGGTGGGGCAGGCTCGGAC ACATTGAGTACATCCTATACATGTATCATAAATCTTTACTGAATGTGACATTGGATCTATACATTTTTGAAC GTCATAAATTTTCGATATAGTAAACTTATAAATGAATGATATATTTAGATACTAGACGAATCAATAAGTTACC AGAATTTTTTTAATCTACTTCTGCAGTGGCTTATGAGAGAAGGCCAAAATACTTCGATTTCTTATGTTTGTG CAAACATGATTAAACCTCACGTAGCAAACATGCTGATTTGAATTAATTTATGAATCTAATATTAGAATTTATA TGATTAATACTACTTATTCAACAAATTCGATTGATTGATACGAATTGATTTTCTGTTACGATAAATTGATGAA ACAATAGCCAGCCCAATAGCTGCTTCAGCGGCTGCAATAGCTATAACAAAAATGGAGAAAATGGCTCCTT TTAATTGACGACTATCAAAAAAATCAGAAAATGTTACAAAATTGATATTAACTGCATTCAAAATAAGTTCAA GACACATAAGGGCCCTAACCATATTTCGACTCGTGATCAATCCATAAAGACCAACAGAAAATAAATAGGCA CTCAAAACAAGTACATGTTCGAGCATCATTAAAAAACTCCTTATCAATCTCGATTCATTTCAATATGAACAA AAATTCAACCGATTCGATTGACTAGAATATAACAAGTACGGAAAAAAGAATATATTGGTAATACACCGAAC TAAAGCATTTCTATTTTATATATGAATTCAAATAAAATTGAAGGGAAATAAATGTGATAACCCAGAACAAAG TTTGATTTGGATTTCCAGTTCGAAAGATTTTTTTATTGACGAGCTACGGCAATTGCACCTATTAACGCAACT AATAGAATTATTGAAATGAGTTCAAATGGAAGAAAAAAATCTGTTGATAGATGAATCCCAATTTGTTGACTA TTGCTTATCAAATCTTGCTCTATAATCTGGTTTGATTTTGTAGTCCAAATAATCCCGTACCATGACGTATCT GGAATAGTAGTAATTAGTGAAACAAAAATACTTGTACAAACCATCGAAGTAACCCCATCTCCAACGGTCCA AAGATGAAAATCTTTGTAATATTCGGAACCATTCATGAACATCACAGCAAAAATAATTAAAACATTTATGGC CCCTACATAAATAAGGAGCTGTGCAGCAGCTACAAAATGGGAGTTCGATAGAATATAGAATAAAGATGTA CAAACAAGAACCAATCCCAATGAAAAGGCGGAATAAATTGGATTGGGAAGTAATACCACTCCCAGACCTC CTAATATAAGACCCGATCCCAGAAAGACTAAAAGAAAATCATGTATTGGTCCAGGTAAATCCATTATATAT AAAATAAGAGATAAATAAATCGAAATCTTTCATAACTTTATTGACCTGGCCAGGAAAAAAAGTCACTCTATT TTTTTATGAAACTTCTTAATTATTAATTCATTATTAATTGAATGAAATTTGAACAGATGTGGATTGATCTAGA TATAGTTATTCGACCTATCTCGCTCTTTATCCGAAAGAGTAGCTTGAAACTATATATTTTAAAATCATTAATA TATAGATTTAAAATCAAAATTAAACCACGATTCTCATCAACAAAACCAATCAATAGTTTGGAGTTGTAGTTA TTCACCAAGTAAAAACCTCACTCTTTTAGATCAAAGGGGAGTCTTATTAATTGGGAATTGCTCTTGAATCAA GAATTTTTTATTTCAGGCAAATTCAAAATAGTTCGAGTTGTGTAATCGTCAATTACTGCCATCGGTAACCGC CCCAAAGCAATTTGATTATAATTCAATTCGTGACGATCATACGTAGAAAGTTCATATTCTTCAGTCATTGAT AAACAATTTGTTGGACAATACTCAACGCAATTACCACAAAATATACAGATTCCGAAATCAATACTGTAATTA AGCAATCGTTTCTTTCGAATATCCATTTCCAATTTCCAATCAACAACGGGTAGATCTATAGGACATACACG AACACATACTTCACAAGCAATGCATTTATCAAACTCAAAGTGAATTCGGCCGCGGAAACGCTCCGATGTA ATCAATTTTTCGTAGGGGTATTGAATAGTTACAGGTAAACGATTCGCGTGTGATAAGGTAATCATGAAACC TTGACCAATATACCTTGCAGCTCGTACTGTTTGTTGACCATAGTTCATGAACTCAGTTACCATAGAGAACA TATCGTGAATATCTATAAAAAAATTTATGCTTGTTTTTTTCTCTTGTTTGAGACAAGTCGTGAAGATATAATA TCGTATTCCTTTACAGTGAAAGAAGTTGGGAAGAGGTTGTTAATAATAGATTGCCGAGAGAAATGGGTAAA AGAAATTTCCATCCAAGATTTAATAGTTGGTCCATTCTTAGCCTTGGTAAAGTCCATCTTGTTGTGATAGGA ATGAATAAGAACAAATAAGTTTTAGCTAATGTAATAAAGATACCAGTTATTGTTCCAAATACTCTACTCGCT TTATTTATTTCAAAAATATCAGGAACGGGTATGTACGGAATAGAAAGATTCCAACCCCCCAAGTAAAGAAC CGTTACAAATAATGAAGAAACTAGTAGATTCAGATACGAAGCAACGTAAAATAAACCAAATTTTATACCTGA ATATTCGGTTTGATAACCTGCTACCAATTCTTCTTCTGCTTCTGGTAAATCAAAAGGTAATCTCTCACACTC GGCTAGGGACGAAATTAGAAAAATGATAAACCCTATAGGTTGACGCCACAAATTCCACCCCCAAAAACCA TATTTTGACTGCGCTTCAACTATATCAACTGTACTTGAACTGTTAGATAATCATAGTCGATGATAACATCAC TGTTCGCATCGCTATTACAGAACCGTACATGAGATTTTCACCTCATACGGCTCCTCGAAGGTCACAAATAA ATCAAAGGACCCGTTCGCTATTATTTATCTTGATATGTTTGTAGGATAGAATCAAAATCTATCGTAAGGTCC CCAATTAGACAATGGAATTCTGTCTGCTATATATTATTTATAATTAAAGTGCTTCTGAATTGATCTTATCTTT TAATAATTTAAAATTTTTCTTTGTTGGGGTAATAACTTAATCTTTTAATAAAATCTTTCTTGCAGCAATATCAA TAAATATTTCAACCTAGCATTTTTGATTACGAGAAAAATTATACATTGCATTAGTTTATGAATCATTACAAGA ATTCTATCTCTCTAAAAAAGATCTCTTTTTGTAGTGCAATTAAATTATTTCTATTTTCTTTTTTCGTTCCTATT CTCCTTTCTCAAAAAGGGGACTTTCATTTAAATATTTAAATAAAGGATTACTTCGTTCTTAATAGTTATTTAC TTAGTCGGTGGATAGGAGCATACTCTGGATCGGAATCGTGGGTAGTACTCCTTCATCATTTCTACCAATTT AAAGCCCCAATTAGAATCCCTTTTATGCAGTATGCACAGAAAAATACTGTTGAATAACTTACATAATCTCTA TTACGAATCCTTTGTGTACCTTGGTGTTCCTAACTATCCACTCATTTTTGGTCAAAAATCCCCATAAAGTTG ATCTTTTGAACCCGCTTCAAGTCATGATGACTAATCAACCAATCTTGGGGTAAACAGTCTCTAATCCTTAT GTTTACTTTCACTTAACTCTTGTACATAGGAAATGAGACTAAAAATTTTTACTGCAAATTTATAAGCCGTTTT TTTCACTCATATAACTATCTGGTTTAGTTCATCAACCCGAATGATGAATAAAAAAATGAAAATATATATTCAA 93 CTCATGAAACTTCCTTCCTAGAAAGAAAAGAAATAGAGAAGATTTATGTCTCAACGAATCACACGTAGAGA TATTGATAACACACATAGAGTTAATGGTATTTCATAACTAATTGATTGAGCAGCAGCTCGTAAACCACCTAA AAAGGAATATTTATTATTTGATCCATAGCCTGACATAAGAAGGCCAACGGGAGCAATACTTGAAATGGCAA TCCATAAAAAAACACCGATACTGAGATCGGCTAGAACAAGGTGATAGCCAAAAGGAATTACTAAATAACTT AGTAGAATTGATGTAACTGCTACGGATGGTCCGATACTGAATAAACGAGTATCTCCTCTCGATGGAAGAA GATTCTCTTTGAAAAGTAATTTTGTACCATCTGCTAAAGCTTGAAGAATTCCCAAAGGCCCGGCGTATTCA GGTCCAATACGTTGTTGTATCCCTGCAGATATTTCTCTTTCTAACCAAACAATTACTAGTACACCTATTGTG ATTCCTAATACAGGAGTAAAAATAGGGATAAGCATCCATATGATCCCATATACCTCTTTTAAGGATTCCAAT CTAAAAAAAGAATTGATAGCTTGTACTTCTGTTGTATCTATTATCATTTTAACGATCAACTTCTCCCATAATT ATATCTATGCTACCTAGTATCGTCATAATATCAGCCAATTTCATTCTTTTAACTAACTGAGGAAGGATTTGC AAATTGATAAAACCCGGTGGTCGGATTTTCCATCTCCAAGGAAAAACACCCCTATCCCCTATCAGAAAAAT CCCCAATTCTCCCTTGGGGGCTTCGACTCTCACATAAAGTTCTTGCTTTGATAATTCAAAAGTCGGAGAAG GTTTTTTACTGATGAATCGATAGTCAAAATCATTCCATTCAGGATCCCGTACTTTATCAAAACGTCGGATTT CTAAATTCTCATAGGGCCCCCCCGGAATTCCTTCTAAAGCCTGTTGAATTATTTTTATGGATTCTGTCATTT CACTGATTCGTACTAAATAACGAGCTAAGGAATCCCCCTCTTTTTGCCATTGGACCCCCCAATCAAATTCG TCGTAAGACTCATAATGATCAACTTTACGAAGATCCCATTGTATTCCGGAAGCTCGTAGCATTGGTCCTGA TAAACCCCAATTTATTGCCTCCGCTCCGCCAATAATGCCTACTCCTTCAACCCGTTCTAAAAAAATAGGGT TCCGCGTAATAAGTTTTTGATATTCAGTAACCCTTGTTAAAAAAAAATCACAAAAATCCAAACATTTATCTAT CCATCCATGAGGTAAATCAGCAGCTACTCCTCCGATCCGAAAATAATTATGCATCATTCGCATACCGGTG GCAGCTTCGAATAGGTCATATATCAATTCTCTTTCTCGAAAAATATAGAAGAAAGGGGTCTGTGCGCCAAT ATCTGCCAGAAAGGGGCCAAGCCATAACAAATGCGAAGCTATACGACTTAACTCCAACATAATGACTCTG ATATAGCTGGCCCTTTTAGGTACTTGAATATTGCCTAACTGCTCTGGTGCATTTACAGTTATTGCTTCTGT GAACATAGTAGCTAAATAATCCCAACGTGTTACATAAGGTAAATACTGTATAATTGTTCGATTTTCCGCAAT TTTTTCCATCCCTCTATGTAAATAACCCAATATTGGTTCACAGTCAATAACATCTTCACCATCTAGAGTAAC AATGAGTCGAAGAACACCGTGCATTGATGGGTGCTGAGGACCCAGATTGACTATCATAAGGTCATTTCGT GTAGCTGATGCAGTCATAGGTTTTTTCCCGATTCATTCTTCCATGAATTGCTGAAAGCGAAAAGAAGTTCA TCAAAATTTAAGCAAAAAATTCAAAATGACTCTTCAAATTAACGAATTTTTGTTTCTCGAATATCCAACCGA CCAATTAATTCTTTATAACGTACTCTATTTTTTTTTGACAAATAAGCCAGTAGCCGTTGACGCTTTCCTAGA ATTTTACGCAGACCTCTCTGAGATAAATAGTCTTTTTTGTGCAATTCCAAATGTGAAGTAAGTCTTCGTATC CTATTGGTGAAACTGACTACTTGAAATTCAGCAGACCCCTTGTTGTCTTTGTTTTCCTCTTTCAAAATAATT CCACTGAATGGATTTTTTATCATAACAGAATCTCCTCCTCCACTTTTTACATATATTATATTAATTTTATTGA TCAGTAATGATAATATCAGTCATTTTTATGTAGTAAACACAAGAATATGAATTTCTTTATGGCTCCCGATTTT CTCAATTAAAACGAATCAATCAAAATTTGAATTTGATTCGGGCAAGGATAAAAAGGGGGACGGTACGTTTT TACACTCACAAACGCGAATAATTTAGACGTAAAATTACGAAGATTCCGGTGATTCATTTATAGATTTTATCC TAACTAATTGATACGTCATTGACTTATTATTATAATATCGTATCGTAGACTGCGATGTAAGACAGGGCATAT GTGCAGCTGTTTGCTTTCATATATGGTACAGAATATATAGTCAAAGTGGACCATCAACGGATTTTTAATTGT GGATATATCCTTAACATACTGAAATTGCTTCCATTATTGGTATCAAACCAATAGCGATTTATACAAGCTAAG TCTTCTAATCGATAATTAGGCCAAAGAAAGAACTTTAATTTAATTAATTCGTTTTTATCTCTATCAAAACGTT TACTTTCATCCAAAAAAGGACTCCTGCTTTTTATGTTCTTCTTGTTGCAAAATACTGGATTGCTATCCACAC CTTTTCTACTCTTTGAATTGAAATAAATTAAAATTCTCAATTCTCTACGACGTCTAGACGATAAAATCTTTTC AGGAACAAGAAAATCATAATGGTTTTTGTCTCTATTTCCAGTTCTTCTTTGATATCTTGGGGTGGATTCATC AAACTTCTTCTTATCAATATATCTTTGTTCTCGGTATCTTTGATTAGTTTGGTACTTACTCTTATGAATTAAT GAAATACCTATGGTTTGATACATAATAAACTGTCCATCCTTTTTTACAGACAGACGAATGGGTTCGATAATC AATACCCCCCTTTTCATCAACTCTCTAACAGTTAAGCTCTTCCGAATCAGCATTATATCCAGACTTATTTCT CTGTTTTGAATAGATGATACAGTAATATTTCTTGGATTTTTCAGTCCAATCAAGAGACAACTTACCTTGATA TTATTGATCATTCTTTGCTTTAAACCCTCGTCGTATCTCAATTGAAAAAGCAAATGCTGTTTCAGTAAGGCA TTTAGTTCTACTTTCATGTTACTTTTGTTCTTGTATTGTTTTTTCGTTTTACCCCTTTTCAGGACCAATTTCG CATAATCTTCTTCAATATCTTTTTGTTGATTTGATAGAACGAATCTGCGATTTCCTTGGTTTTGTGCATCTG ATCCAAGATCTCCTCGGCCTGCGGGTTCTTTTTCTTCTTGATTTCGATTCTTTAATTCAAAATTTTTTTTTTC ATTTGATGGTCTAAAAAAATTCCATTTTTGCTTTCCATTGATGTTTTTATTGTCACTAACATTTTCATTTCTAT TCAAATTTAAAAGAAGTAATTTGCTTGGTATAATCCACGGTTTTTTTTTGTATACATTATAAAGTAGCACAAA TTCTGGGAAGAACCAAAGTTCCAGATTCGATATGTGGCGATTTAGTATTTCTTCATTCATTTCCATCCAAC CAAAAAGGTGTTTTTTCTGATTGAGCGGATTTTTTTCTAAATCTTGATAAAGCGTAAGATAAAAAATATCGT TCTTATGAATTTTATCAATTTTTTGGTAATTCTTACTTTCAATCTTAGTATTTTTATTACTGTTGGGATCCATT TTGATCCAGGTCTCAATATCAACTTTTTCTCTTAAAAAAAAGTTGATAATTTTCCAATCAAAATATTTTCTAT CTGGATTTTTTTCCATATACATAATATCACCCTTTCCTAGATAATTATTGATAGGAATATCCTCTAGTATATC AAAGAATTTTTGTTTCTGTGTGGTGTAATTATAAGAAATTCTTTGGTTGTTATTTACTTGCAATGGTGATACA TAAATAATATATGAGTCCGTCTTCCTTTCATAATTAATAGATTTATATGATAAAAGGTCATATCTATAGTATT 94 TTTGAAAATTCTCGTTTTTATTTGGGTTTGGTAATGAATATACTTCAAAATCGTTTTGTTTTTTGTAATGAAG TAATCGGTCTTTTGAATCCCATTTTGTTAAATTTTTTTTTTGAGTTATACGGCCTTGATTGATTGTATTTCGC CATTTTTGTGGTATTAATCCAGACCATCTAATCTGAGATAAATTGTATTGATAATGACCTCTTAACCAGTTT TTCCATTGATTCGTTCCATAACTTGGAAATTTCGTATGCTCTAATTCGGAATGAAATATTCCTTGTGTTCCA AAAGAATCCTTTATTGCAGTCTTAAGAAAAAAAGAAGTTCCATGATATTCAAGAACAGATCTTAACTTATAT AAATTAATAACTCGGGTTTGGGATAATTTGTAAAATACATATGCTTGCGACAAGGAGGATAAGTCAAAAAA AAGATGTGAATTTTTCTTACTATTACTAATATTATAAAGTGACTTTTTTATAGTCGAAATAAAGTGAATTGTA TTTTGATTTGTTTTATTAATTGTTTCTTGGTTTGTTTCATTATTGTAAATGTATTTATATTTTTGAATAATTTTT TTTGTTGATTCAAGAACAAGTTGTGTATACATTCTGGCAATATTAATGATATATAGAAAGATATCTATGTATA TTTTTTCAATAAAAATTTTTATAAAAACCTGTAATTTCCAGATTAATCGAGTATTTCTTCTTTTTAATATTTGC CAAATATCTTTTGGCCATTCTACATTATAACTTCTTTTATTAGGACTACTGTTTATTCCTGGAGTTCCTTTTT TTTTTTTTTTTTCTTTTGTAATACTCTTTATTTTCTTTCTGATTGTGCTTGTTCTATCAGTTATATTTTTAATTT TTCTTTCTCTCGGTGAATAATTATCTGTAGATCGAATTTTATTAAACGAGTTATGAATTGTCTGATTGCTGA TTATAGAACCTTTTTCTTGGTTAGTTTCACCGGATTCATATACTTCTTTCAATCTAAATAGAGTTGGATTTGC TTTTGAGAGCTCTTTTTTTTTTCTTTTTAGAAACAGAAATAAAACGTTTTTGATAAACCCCCTTTTTGTTCTT CTTATTAAAACTCTTCGAGTTAGAAAATCCTTAAAAATGGGCTCAAAAAAGGAAGTTATTTTTCGGGGAGA ACCAAAAGGAAGGTCAGTTTCCATTCCACTAGCCGTTAAAAAACAAGAATTATCCCCTTTTTGCTCTTTTTT TAGATCTCTATAAGAGGGCCGCAACTTAGGCTTAGATCTGTACCAAGGTTTCAAACAGAAGGGAAATAGT ATTTTTATCTGAATACCCTCTGTTAACCAATTTGCGGGAAGTTCTGTTTCTGATACTTGAACACCGTTATAG GTACATTTAATATGCTTTTCTCTCTTCCATTCATGAAAATCCTCAGACCACTCAGGGGGTTGGAATAATAA GATACGCCCAATATTTTTAACTATTATCAATGAAGGTAATATAATATATTTTCTAAGCATCGATTGAATTATT AATAGCAAACTTCTTGTTGTTTTCGAAAATGGAACGAGATCCCAGATTTCCGGCAGTTCTATCCGCACTTT TTCCTTTATGTTGTTCTCCTCTTGTTTCTCTCTTCTTTTTGTCTGTTCTTCTGTATAATCTTTTAATTCTGCCC CTTTACCCATCCAATTTCTAAAGATAAGTTTCATCAGTTCGGAGATATCAAAAGAAAAAAGGGGGGATTTT TTTCTTCTGTTCAAAAAAAGTGGGGAGTGCGCATTTGCTTCAAAGAGTTTCCAAATAATAGTTTTACGCCTT TGAGTACGCATAGAACCTTTGATTATGTCTCGACGAAAATCCGATTCTTGCAAATATTCTATCGTATATAGC GGGCCTTTTTTATCAATATTTTTAGAATTCCAGTTGTTATCAGTAAAAAGTACTATATCGTCAATTTTTCTTG AACGAATCTGATGGCCCACCCGTACGCCTTCTTGAATTACGCCCGTCTGTTGTTCCAACTCGGTAATAAA TTTGTCTGACTTTCGAGGGACTTTTTTACTGATTTCTTTTATTCCAAAAGATTTTTGTTTTATTTTGTGACCA TTAGCGATAGTTATAATTGCATTTAACAAAAATTTTAGAAGTTTTGCTTCATTTTCTGAAAATAAGGAAGGG CCCTTCAAATTTAAACCCGATCTTGATTCTCTATCAAATTTACTGATTAAAGTAAATAAATGACTAATTTCTG TTGAAAATATTTTTTTTTTTTCAAATGGATCTATTTTCTGTTTAAACTCTTGGTAATCGGTATCAGGAAGAAG GAGATTATGAATCTTATTTATTTCCATTGTCTCTATGAAATTTTCTAACGAAATTTTATTTCTGATTGAAGGT GAAAATTTTTTTTTGATTGTTCCACGATATGACCCATTCAAAATGGGATCATATTTTTTAGGCAAGTAATCTT TTTTAGTGTCATTATACAATCTAGTACTTTTTTCGAGTATATCTAGAGAAAGAAATCCCATATCTAGAGCCT TAATTCTATTTAAAAACTCGTTGTTTAAGTTGTTCTTTTTTTGTTTGTTAGTATAAATCCAATGATTGTACAAT TCATCCGAAGAGAGTTTTTCTAGTGTGGGCAAGGACATCCTTCTTTGTATCATTTCTCCAAAAGTTGACAA GCTAGGCGGATACGTAAAAGATATTCTTTCTTTTCCATCACTTCGGCATGTATAAAAAAAATATTGTGACAT TTCATTTCTTGCAGTCCTTTCAAATCTATTATTTTTTATGTATCGTAATGGGCGATTCCATCGTTTATAATCG AAAAGAAGGGTCACAAGAGGTTTTTCAAACCAGAAGAGGCCTTTATCTTCATCTTTTTTATCAAGTATTTTT ATTTTGTTAAAATTTTCTTGATTCTTATACAAATAAGAAACTTTATAAACTGGGCTATTGTTATAGGATGTCT CTGTAAAGTGGAATTCATCCTTTGTTTTTTCCTTTCCATTCACTCGGATCTCTTCCGTTTCATCGATTTTGT CCGGATCCTCCTTTTCTTCCGAAAAAAGGGAAGGAGAAGGATCTTCTTCGGTGGATCCCTCTTGTTCCTG TTTAGCCCCCTTCGTTTCGGAAGCTGTTTCTATTTCAACATCTGTTTCTTCCTCACTTTCCCCCCTTTCTTC CGTTGTTGGGGTTTCTTTCAGTTTCTTAGTAATAATGGGTGACGGTATTCTGCCTAAATAGTAGACACAGG TAATAAATAAGAGAATACTAAAGATTCGAGCCATAGAATTTATCAATTCTGACACAAGGTACTTATTAGATC GAATAAGTACATTCGATCTAATAGAATTATTTTGCCGTATCCAGACTAATACCAATCCAACCCATTTCATGA ATAAAATGTGACCAATTAACCAACCAACAAAACTGCTTGTTACAAATAACATCTTGTTGTTGCATCGAAACA TATAAATGTTGACTAATCTGGCTAACATTGAACTTGGTAAAATGAAATGGTTGAATAATTGAAAAATGAGAT TATTCAGGAATACGCATTGAATGCTAAGATTACGCATTGAATTTCTGGTAGTAGATCCATAATCAAAAAAGT TTTTGTGATTATTCCAGAAGAAATGAAACAAAAGATACGGTAGAGCTAGTACAGTTATTGTATGAGGTCTA CCCAATGCTAGATGCAGAGGCGCATAATAGATCGATATGAACATCATGAGCTGTCCCGTAATAAAACCTG TTGTTGCTGATACCTTCTTCTCGGTTCCTTCTTCTCCTTCTTCCATAACCCGAGCTCGGAGAAGGAAGAGA TAAGAGGGCCCTATGGAGAATGTGGTCAGAAATCCATAATAGAGTCCGACCACAACGACCGAATTGATTA TCTTCATGCATAAGGATACTAGATTACCTAGTAGAAAAGATTTCAAAATCATCACAAACCTCCCTTTTTCTT TTCTATTGCAATTTCTGGATTATTATATGATGATTTTTGAACTTTCCATATATAGAAAAGAAATAGAAAGAGA TAGACTAGAAACGACATCTGTTATGTCAATGACACCAAAGGGATATTAAATGAATGGAATTGGGATATGGA TGGAATATAATGAAATAGAGCCACTTTGAGGTTCCCTATGAAATGAGGCATGGAAGGGGGCCACTACGAA 95 GAAGTTCCGGGAGTTACGAAGGAAGCTTCGAGCTCATATTGGTCATGGGTTGAGAACGGGAATTGAACT CTATGAGATCTAATCTCTCGTTGTTCCTCAGTAGCTCAGTGGTAGAGCGGTCGGCTGTTAACTGACTGGT CGTAGGTTCGAATCCTACCTGGGGAGATTTGATTCATTCTGAATTCTTTAATTCAGAATAAAAGGGCTCGC TTTGACCGTTAAGAGTAGGTAACCCATTCCCCGTCTTTGTTTCTATTGCATTCTATCTCATCGTATCACATC CTGTTCTGCGAGATTTTAAAATCACCGTCAATACCTCGGTGTAGGTCCGGGATAATCCTTTGTTCCATAGC CCTGGGGCTATTTACAACTATCCAATTAAGAATTCTCAGATGTACTAGTACTAGCAGTGCATCAAAGATGC AGTCATCGATTCTCCCGAGAGGCCACAATTGCCGTGAGCAAACATATTAATGACGAGGAACGCATTTTTG TTATGCTACTAATACTTGTACTTGCTCTGCTATTCTGCCCCAGCCTGGCTGAGGAAGAGTTACGGGGCGT AAAACAAAAAATACGCTGAGGGGGGTCGGGCATACTATGTGTAATGAATAAACCATTTACGATAAATAAAA TAAAAAAAAAGGGCCATTCCATTTCGACAAAAGACCCACACCCAAGTTCCATAGCTTTTGGTCCGCTATCC CGATCATGATTTTCCTACCCCCAGAGGGAAAGGCCCTTCCCTTTTGGGCCGGTTGTGGGCGAGGAGGGA TTCGAACCCCCGACACCGTGGTTCGTAGCCACGTGCTCTAATCCTCTGAGCTACAGGCCCCACCCCGTC TCCACTGGATCTGTTCCCGGGAGTACCCTCAAAAAAAGGAACCTTTCCTCTCCCCAGCCATTTCGGGTTA AGAAGATGTGAAAGCGCCTTTCTCTCTATACGAACGGAACGGTGCGTTCCGAGGTGTGAAGTGGGAGAG AGGGGATGTCATAATTGGGGTTTTGAATAAGACGACCTTTTGATTGATTTTTCATTTATTTATTTTTCATATT GAAAAATAAATAAGAATGGGAGGTGTTAAGCTTTTTATCATCCTGGCGTCGAGCTATTTTTCCGCAGGACC TCCCCTACAGTATCGTCACCGCAGTAGAGTTTAACCACCAAGTTCGGGATGGATTGGTGTGGTTCCTCTA CGCCTAGGACACCAGAATATCGAACCATGAACGAAGAAAGGCATGAGAGAAAAGCATATTGGCTAGTGAT TGTGAGGCCCCAATTCTTGACTGGAGGGGACACCAAAGGCCTCTGCCCTTCCATCCCTTGGATAGATAG AGAGGGAGGGCAGAGCTTTTGGTTTTTTGTTGAACAAGGGTTTTTTCGTGTTGTCAAAGAGTTGAACAAT GAAAATAGATGGCGAGTGCCTGATCGAATTGATCGGGTCATGTAGGAACAAGGTTCAAGTCTATCGGTCT GTTAGGATGCCTCAGCTGCATACATCACTGCACTTCCACTTGACACCTATCGTAATGATAAACGGCTCGT CTCGCCGTGACCTTCTCTTGAATTCTCAAAACTTCTGTCGCTCCATCCCCGCAGGGGCAGAGAACCCGTC GCTGTCTCGGCTGTGCTACCGGAGGCTCTGGGGAAGTCGGAATAGGAGAGCACTCATCTTGGGGTGGG CTTACTACTTAGATGCTTTCAGCAGTTATCCGCTCCGCACTTGGCTACCCAGCGTTTACCGTGGGCACGA TAACTGGTACACCAGAGGTGCGTCCTTCCCGGTCCTCTCGTACTAGGGAAAGGTCCTCTCAATGCTCTAA CGCCCACACCGGATATGGACCGAACTGTCTCACGACGTTCTGAACCCAGCTCACGTACCGCTTTAATGG GCGAACAGCCCAACCCTTGGAACATACTACAGCCCCAGGTGGCGAAGAGCCGACATCGAGGTGCCAAA CCTTCCCGTCGATGTGAGCTCTTGGGGAAGATCAGCCTGTTATCCCTAGAGTAACTTTTATCCGTTGAGC GACGGCCCTTCCACTCGGCACCGTCGGATCACTAAGGCCGACTTTCGTCCCTGCTCGACGGGTGGGTCT TGCAGTCAAGCTCCCTTCTGCCTTTGCACTCGAGGGCCAATCTCCGTCCGGCCCGAGGAAACCTTTGCA CGCCTCCGTTACCTTTTGGGAGGCCTACGCCCCATAGAAACTGTCTACCTGAGACTGTCCCTTGGCCCG TAGGTCCTGACACAAGGTTAGAATTCTAGCTCTTCCAGAGTGGTATCTCACTGATGGCTCGGGCCCCCCC GGAAGGAGGCCTTCTTCGCCCTCCACCTAAGCTGCGCAGGAAAGGCCCAAAGCCAATCCCAGGGAACA GTGAAGCTTCATAGGGTCTTTCTGTCCAGGTGCAGGTAGTCCGCATCTTCACAGACATGTCTATTTCACC GAGCCTCTCTCCGAGACAGTGCCCAGATCGTTACGCCTTTCGTGCGGGTCGGAACTTACCCGACAAGGA ATTTCGCTACCTTAGGACCGTTATAGTTACGGCCGCCGTTCACCGGGGCTTCGGTCGCCGGCTCCCCTG TCATCAGGTCACCAACTTCCTTGACCTTCCGGCACTGGGCAGGCGTCAGCCCCCATACATGGTCTTACG ACTTTGCGGAGACCTGTGTTTTTGGTAAACAGTCGCCCGGGCCTGGTCACTGCGACCCCCTTTGTGAGG AGGCACCCCTTCTCCCGAAGTTACGGGGCTATTTTGCCGAGTTCCTTAGAGAGAGTTGTCTCGCGCCCC TAGGTATTCTCTACCTACCTACCTGTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAG CTTTTCCTGGGAGTATGGCGTGGGTTACTTCAGCGCCGTAGCGCCTGGTACTCGAACATTGGCTCGAGG CATTTTCTCTACCCCTTCTTACCCTGAAAAAGCAGGGTCACCTTACGTCCTTGAACCGATAACCATCTTTC GGCTAACCTAGCCTCCTCCGTCCCTCGGGACCAACAAGGGGTAGTACAGGAATATTCACCTGTTGTCCAT CGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCACCCTCCGTGGACGAACCTTGCGGAGGAACCC TTAGGTTTTCGGGGCATTGGATTCTCACCAATGTTTGCGTTACTCAAGCCGACATTCTCGCTTCCGCTTCG TCCACTACCGCTCGCGCGGGTACTTCCCTCTAAGGCGGAACGCTCCCCTACCGATGTATTTTTACATCCC ACAGCTTCGGCAGATCGCTTAGCCCCGTTCATCTTCGGCGCAAGAGCGCTCGATCAGTGAGCTATTACG CACTCTTTCAAGGGTGGCTGCTTCTAGGCAAACCTCCTGGCTGTCTCTGCACCCCTACCTCCTTTATCAC TGAGCGGTCATTTAGGGGCCTTAGCTGGTGATCCGGGCTGTTTCCCTCTCGACGATGAAGCTTATCCCC CATCGTCTCACTGGCCGACCTTGACCCCTGTTATTTTGAGGTCATATCTAGTATTCAGAGTTTGCCTCGAT TTGGTACCGCTCTCGCGGCCCGCACCGAAACAGTGCTTTACCCCTAGATGTCCAGTCAACTGCTGCGCC TCAACGCATTTCGGGGAGAACCAGCTAGCTCTGGGTTCGAGTGGCATTTCACCCCTAACCACAACTCATC CGCTGATTCTTCAACATCAGTCGGTTCGGACCTCCACTTAGTTTCACCCAAGCTTCATCCTGGTCATGGAT AGATCACCCAGGTTCGGGTCCATAAGCAGTGACAATTGCCCTATGAAGACTCGCTTTCGCTACGGCTCC GGTGGGTTCCCTTAACCAAGCCACTGCCTATGAGTCGCCGGCTCATTCTTCAACAGGCACGCGGTCAGA GCCCTGGCTCCTCCCACTGCTTGGGAGCTTACGGTTTCATGTTCTATTTCACTCCCCGATGGGGGTTCTT TTCACCCTTCCCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTTGCAAGGTGGTCCT 96 TGCTGATTCACACGGGATTCCACGTGCCCCATGCTACTCGGGTCAGAGCGTAAGCTAGTGATGCTTTCG GCTACTGGACTCTCGCCATCTAGGGTGCAGCACTCCACCGCTTCGCCTAGCAGCACGACGCTTGTATTG CTCTCCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCTACTACGGGAATCGCTT TTGCTTTCTTTTCCTCTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCTTGCCTGCCCATGGATTCA GCAGCAGTTTGAAAGGTTGACCTATTCGGGAATCTCCGGATCTATGCTTATTTTCAACTCCCCGAAGCATT TCGTCGCTTACTACGCCCTTCCTCGTCTCTGGGTGCCTAGGTATCCACCGTAAGCCTTTCCTCGTTTGAA CCTCGCCCTTAACTTTAAGGCTATGCCATCCTAAGGTGCTGCTAAATGGATGGATCTTATCAACGTCCATG AATGATAAATCATAGATCGAACCGCCGAATCGGAAAAATTGGGTGCTATCATATAGCTTTGTATCGGCTAA GTTCACGAGTTGGAGATAAGCGGACTCGAACCGCTGACATCCGCCGCAGGGTAAACCACCGCCTCTCAG GTCCCCCGACTGATTCTACCATAGAGACCAACGATAGACAATAACTCCCCCCCGAACACAGCTTACAACT TTCATCGTACTGTGCTCTCCAAAGAGCAACTCTTCTCAAAATCTCACTCAAAAGGTGCTGAGTTGGAATCC CATTCTAACTAAGGATTCTTGTGGTTCCGGAGGATCCAGCTACAGGAGAACCAGGAACGGAGAGCTTCC CCCCCCCCTTTCCGCCGACTCTTTGGTCTTAAGAACGCCGGTTTTAAGAATGAGTGATTGCCCTTCTCCG ACCCTTACTGCCCAACCTGAGAGCGGACAGCTAATGCGTTCCACTTATTGAACAGGGTTCTATGGTCGGT CCGTGACCCCTGGATGCCGAAGGCGTCCTTGGGGTAATCTCGTAGTTCCTACGGGGTGGAGATGATGG GGTCGGTCCATGGATTTTCCTTCCTTTTTCCGCATTTCGCTCAAAGGGTTGAAGGGAGATAGTGCATCAA GCTGTTCGCAAGGCCAACTTGATCCTCTTCCCCAGAGATCCCAGATGAGGGAACCCTAGGAGAGCCGCC GACTCCAACTACCGTCCATGTACGATCCATACTAGATCTGACCAACTGCCCATCCTACCTCCTCTACGTTC TTGACAGCCCATCTTTGTCTCAGTAGAGTCTTTCAGTGGCATGTTTCGGTCCTCTTCCCCATTACTTAGAA AAAGTGAGCCACCGGTTCAGGTACAAGATACTATCATTACCGCCTGGACAATTAGACATCCAACCCGTAA TCGCAACGACCCAATTGCAAGAGCGGAGCTCTACCAACTGAGCTATATCCCCCCGAGCCAAGTGGAGCA TGCATGAAGGAATCAGATGCTTCTTCTATTCTTTTCCCTGGCGCAGCTGGGCCATCCTGGACTTGAACCA GAGACCTCGCCCGTGAAGTAAATCATCGCACCTACGGTCCAACCAATTGGGAGGAGAATCAATAGATTCC TTTTCGGGAGCGATTCATCCTTCCCGAACGCAGCATACAACTCTCCGTTGTACTGCGCTCTCCAAGTGTG CTTGTTCCCCCCTTCTTCCTTACCATGGCAAGTCTTTGTGAAAGAACTCCGATGAGAAGAAAAAAGAAGG CGTTAAGAGACCCTCCTGGCCCAACCCTAGACACTCTAAGATCCTTTTTCAAACCTGCTCCTCCCATTTCG AGTCAAGAGATAGATAAATAGACACATCCCGTTGCACTTATCGGGGGCGTTCGTAGTGACTGAGGGGGT CGAAGACCAAGAAGTGAGTTATTTATACCAAGCATTCTTCTTACGGCTAGATCCAATCTCCTGGTCCCTGC GGAAAGGAAAAAGAATTTCACGCTCTTCCTTTCGGTAAGGGAGGATTAGGGAAATCCTATTGATTGCAGC TTTCTCCAGACCTCCGGGAAAAGCATGAAAAAAACGGCTCGAATGGTACGATCCCTCCGTCACCCCAGAA TGAAAGGGGTGATCTCGTAGTTCTTGGTCTGTGAAGATGCGTTGTTAGGTGCTCATTTTATTTTCCCATTG AGGCCGAACCTAAACCTGTGCTCGAGAGATAGCTGTCCATACACTGATAAGGGATGTATGGATTCTCGAG AAGAGAGGAGCCATGGTGGTCCCCCCCGGACCGCCCGGATCCCACGAGTGAATCGAAAGTTGGATCTA CATTGGATCTCACCTGAATCGCCCCATCTATCCTCCTGAGGAGAAGTTTGGTTTCAAACCCCGGTTCGAA CAGGAGGAGTACGCCATGCTAATGTGCCTTGGATGATCCACATCTCAGGGTCAGGCGCCGATGAGCACA TTGAACTATCCATGTGGCTGAGAGCCCTCACAGCCCAGGCACAACGACGCAATTATCAGGGGCGCGCTC TACCACTGAGCTAATAGCCCGTCGTGCGAGCCCCCCACTGGGGGCCCGCTATGCCAAAAGCGAGAGAA ACCCATCCCTCTCTTTCCCTTTTTCGCCCCCATGTCGCCACACGGGGGGGCATGGGGACGTAAAAAAGG GGATCCTATCAACTTGTTCCGACCTGGGATAATAAGCTCATGAGCTTGGTCTTACTTCACCATCGAGAAAC GAAAGAAGACTTCCATCTCCAAGTTTCACTCAGACGTAGCTCCCTTCTTTTTGGGTGTGAAGCAGTGTCAA ACCAAAATACCCAACAAACATTAGCTCTCCCTGAAAAGGAGGTGATCCAGCCGCACCTTCCAGTACGGCT ACCTTGTTACGACTTCACTCCAGTCACTAGCCCTGCCTTCGGCATCCCCCTCCTTGCGGTTAAGGTAACG ACTTCGGGCATGGCCAGCTCCCATAGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGAATTCACCG CCGTATGGCTGACCGGCGATTACTAGCGATTCCGGCTTCATGCAGGCGAGTTGCAGCCTGCAATCCGAA CTGAGGACGGGTTTTTGGAGTTAGCTCACCCTCGCGGGATCGCGGCCCTTTGTCCCGGCCATTGTAGCA CGTGTGTCGCCCAGGGCATAAGGGGCATGATGACTTGACGTCATCCTCACCTTCCTCCGGCTTATCACC GGCAGTCTGTTCAGGGTTCCAAACTCAACGATGGCAACTAAACACGAGGGTTGCGCTCGTTGCGGGACT TAACCCAACACCTTACGGCACGAGCTGACGACAGCCATGCACCACCTGTGTCCGCGTTCCCGAAGGCAC CCCTCTCTTTCAAGAGGATTCGCGGCATGTCAAGCCCTGGTAAGGTTCTTCGCTTTGCATCGAATTAAAC CACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCA GGCGGGATACTTAACGCGTTAGCTACAGCACTGCACGGGTCGATACGCACAGCGCCTAGTATCCATCGT TTACGGCTAGGACTACTGGGGTATCTAATCCCATTCGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTC GGCCCAGCAGAGTGCTTTCGCCGTTGGTGTTCTTTCCGATCTCTACGCATTTCACCGCTCCACCGGAAAT TCCCTCTGCCCCTACCGTACTCCAGCTTGGTAGTTTCCACCGCCTGTCCAGGGTTGAGCCCTGGGATTT GACGGCGGACTTAAAAAGCCACCTACAGACGCTTTACGCCCAATCATTCCGGATAACGCTTGCATCCTCT GTATTACCGCGGCTGCTGGCACAGAGTTAGCCGATGCTTATTCCCCAGATACCGTCATTGCTTCTTCTCC GGGAAAAGAAGTTCACGACCCGTGGGCCTTCTACCTCCACGCGGCATTGCTCCGTCAGGCTTTCGCCCA TTGCGGAAAATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCTGAT 97 CATCCTCTCGGACCAGCTACTGATCATCGCCTTGGTAAGCTATTGCCTCACCAACTAGCTAATCAGACGC GAGCCCCTCCTCGGGCGGATTCCTCCTTTTGCTCCTCAGCCTACGGGGTATTAGCAGCCGTTTCCAGCT GTTGTTCCCCTCCCAAGGGCAGGTTCTTACGCGTTACTCACCCGTCCGCCACTGGAAACACCACTTCCC GTCCGACTTGCATGTGTTAAGCATGCCGCCAGCGTTCATCCTGAGCCAGGATCGAACTCTCCATGAGATT CATAGTTGCATTACTTATAGCTTCCTTGTTCGTAGACAAAGCGGATTCGGAATTGTCTTTCATTCCAAGGC CTAACTTGTATCCATGCGCTTCATATTCGCCCGGAGTTCGCTCCCAGAAATATAGCCATCCCTGCCCCCT CACGTCAATCCCACGAGCCTCTTATCCATTCTCATTCAATCACGGCGGGGGAGCAAATCATAATAGAAAA ACTCACATTGGGTTTAGGGATAATCAGGCTCGAACTGATGACTTCCACCACGTCAAGGTGACACTCTACC GCTGAGTTATATCCCTTCCCTGCCCCCATCGAGAATAGAACTGACTAATCCTAAGGCAAAGGGTCGAGAA ACTCAACGCCACTATTCTTGAACAACTTGGAGCCGGGCCTTCTTTTCGCACTATTACGGATATGAAAATTA TGGGAAAAGTCGGATTCAGTTGTCAACTGTCCCTATCGGAAATAGGATTGACTACGGATTCGAGCCATAG CACATGGTTTCATAAAACCGCACGATTTTCCCGATCTAAATCAAGCAGGTTTTACATGAAGAAGGTTTGGC TCGGCATGTTCTATTCGATACGGGTAGGAGAAGAACCCGACCCGGTATTATTAAAAAAATAGAGGAAGCA GAACCAAGTCAAGATGATACGGATCAACCCCTTCTTCTTGCGCCAAAGATCTTACCATTTCCGAAGGAAC TGGAGTTCCGTCTCTTTTCCATTTCCATTCAAGAGTTCTTATGTGTTTCCACGCCCCTTTGAGACCCCGAA AAATGGACAAATTCCTTTTCTTAGGAACACATACAAGATTCGTCACAAAAAGGATAATGGTAACCCCACCA TTAACTACTTCATTTATGAATTTCATAGTAATAGAAATACATATCCTACCGAGACAGAATTTGTAACTTGCTA TCCTCTTGCCTAGCAGGCAAAGATTGACCTCCGCGGAAAGAATGATTCATTGGGATCGACATGAGAGTCC AACTACATTGCAGTGCCAGAATCCATGTTGTATATTTGAAAGAGGTTGACCTCCTTGCTTCTCTCACGGTA CAACCCTCTTCCCGCTGAGCCCCCTTTCTCCTCGGTCCACAGATACAAAATGTAAGACTGGCGCCAACAG TTCATCACGGAAGAAAGGACTCACTAAGCCGGGATCACTAACTAATACTAATCTAATCTAATAGAAAATAC TAATATAATAGAAAAGAACTGTCTTTTCTGTATACTTTCCCCGGTTCCGTTGCTACCGCGGGCTTTACGCA ATCGATCGGATTATATAGATATCCCTTCAACACAACATAGGTCATCGAAAGGATCTCGGAGACCCACCAA AGCACGAAAGCCGGGATCTTTCAGAAAAAGGATTCCTATTCAAAGAGTGCATAACCGCATGGATAAGCTC ACACTAACCCGTCAATTTGGGATCCAATTCGAGATTTTCCTTGGGAGGTATCGGGAAGGAATTGGAATGG AATAATATCGATTCATACAGAAGAAAAGGTTCTCTATTGATTGTACCTATGGGATATAGGAAGAGGAAAAA CCGAAGATTTCACATAGTATTTTTGATCGAAAAATCAATCTTATTTATTTCGTACCCTTCGCTCAATGAGAA AATGGGTCAAATTCTAAAGGATCAAACCTATGGGACTTAAGGAATGATATAAAAAAAGAGAGGGAAAAATA TTCAATAAAAAGAAATAAAAAGAAAATAAGTAAACTAAGTAAATATAGAAGAACCCAGATCCCAAATGAACA AATTCAAACTTGAAAAGGATCTTTCTCATTCTCGAAGAATGAGGGGCAAAGGGATTGATCGAGAAAGATCT CTTGTTCTTATTATAAGATCGTGATTGGATACGCATATGTTTGGTAAAGAGAATAATCTTCTCCTTTGAGTT TGAGAATAATCCAAAATGGAAAGTGTTCAATTGGAACATGAAAACGTGACTAAATTGGTCCTAGTTACTCT TCGGGACGGAGTGGAAAAGGGGGGGATTCTCGAACGCGGAAAAGGATCCAATGACTTCGAAAGAATTGA ACGAGGAGCCGTATGAGGTGAAAATCTCATGTACGTTTCTGTAGAGTGGCAGTAAGGGTGACTTATCTGT CAACTTTTCCACTATCACCCCCAAAAAACCAAACTCTGCCTTACGTAAAGTTGCCAGAGTACGATTAACCT CTGGATTTGAAATCACTGCTTATATACCCGGTATTGGCCATAATTCACAAGAACATTCTGTAGTCTTAGTAA GAGGGGGAAGGGTTAAGGATTTACCCGGTGTGAGATATCACATTGTTCGAGGAACCCTAGATGCTGTCG GAGTAAAGGATCGTCAACAAGGGCGTTCTAGTGCGTTGTAGATTCTTATCCAAGACTTGTATCATTTGATG ATGCCATGTGAATCGCTAGAAACATGTGAAGTGTATGGCTAACCCAATAACGAAAGTTTCGTAAGGGGAC TGGAGCAGGCTACCATGAGACAAAAGATCTTCTTTCTAAAGAGATTCGATTCGGAACTATTATATGTCCAA GGTTCAATATTGAAATAATTTCAGAGGTTTTCCCTGACTTTGTCCGTGTCAACAAACAATTCGAAATACCTC GACTTTTTTAGAACAGGTCCGAGTCAAATAGCAATGATTCGAAGCACTTCTTTTTACACTATTTCGGAAAC CCAAGGACTCAATCGTATGGATATGTAAAATACAGGATTTCCAATCCTAGCAGGAAAAGGAGGGAAACGG ATACTCAATTTCAAGTGAGTAAACAGAATTCCATACTCGATCTCATAGATACATATAGAATTCTGCGGAAA GCCGTATTCGATGAAAGTCGTATGTACGGCTTGGAGGGAGATCTTTCATATCTTTCGAGATCCACCCTAC AATATGGAGTAAAAAAGCCAAAATAAGTGATTTTAGCCCTTATAAAAAGAAAACTTATTCTTGAACCCCTTT CACGCTCATGTCACGTCGAGGTACTGCAGAAGAAAAAACTGCAAAATCCGATCCAATTTATCGTAATCGA TTAGTTAACATGTTGGTTAACCGTATTCTGAAACACGGAAAAAAATCATTGGCTTATCAAATTATCTATCGA GCCGTGAAAAAGATTCAACAAAAGACAGAAACAAATCCACTATCTGTTTTACGTCAAGCAATACGTGGAGT AACTCCCGATATAGCAGTAAAAGCAAGACGTGTAGGTGGATCGACTCATCAAGTTCCCATTGAAATAGGA TCCACACAAGGAAAAGCACTTGCCATTCGTTGGTTATTAGCGGCATCCCGAAAACGTCCGGGTCGAAATA TGGCTTTCAAATTAAGTTCCGAATTAGTGGATGCTGCCAAAGGGAGTGGCGATGCCATACGCAAAAAGGA AGAGACTCATAGAATGGCAGAGGCAAATAGAGCTTTTGCACATTTTCGTTAATCCATGAACAGGATCTATA CATCTCGATCGGAAAAGAATCAAGAGAAAAAGAAAGAATCGGAATTGATCGATATATTTCTCGAAACAAAC GAAAAGGAAACGAAAGATGAAACAAAAATCATGGATCAACTAAGCCCTCTCGGGGACTTTCTTAAGAATA AGAAAGAGGAACCTCATGTAAATACCATGGAATAAGGTTTGATCCTATTCATGGAGATTCCGTAAATATTC CATTCCAAAAATGGAAAGTTCGAAACAATTGGGATTTTTTTGGAAATTGGATGCAGTTACTAATTCATGATC TGGCATGTACAGAATGAAAACTTCATTCTCGATTCTACGAGAATTTTTATGAAAGCCTTTCATTTGCTTCTC 98 TTCGATGGAAGTTTGATTTTCCCAGAATGTATCCTAATTTTTGGCCTAATTCTTCTTCTGATGATCGATTCA ACCTCTGATCAAAAAGATATACCTTGGTTATATTTCATCTCTTCAACAAGTTTAGTAATGAGCATAACGGCC CTCTTGTTCCGATGGAGAGAAGAACCTATGATTAGCTTTTCGGGAAATTTCCAAACGAACAATTTCAACGA AATCTTTCAATTTCTTATTTTACTATGTTCAACTCTATGTATTCCTCTATCCGTAGAGTACATTGAATGTACA GAAATGGCTATAACAGAGTTTCTCTTATTCGTATTAACAGCTACTCTAGGAGGAATGTTTTTATGCGGTGC TAACGATTTAATAACTATCTTTGTAGCTCCAGAATGTTTCAGTTTATGCTCCTACCTATTATCTGGATATAC CAAGAAAGATGTACGGTCTAATGAGGCTACTATGAAATATTTACTCATGGGTGGGGCAAGCTCTTCTATTC TGGTTCATGGTTTCTCTTGGCTATATGGTTCATCCGGGGGAGAGATCGAGCTTCAAGAAATAGTGAATGG TCTTATCAATACACAAATGTATAACTCCCCAGGAATTTCAATTGCGCTCATATTCATCACTGTAGGAATTGG GTTCAAGCTTTCCCCAGCCCCTTCTCATCAATGGACTCCTGACGTATACGAAGGAGTGCGGTTCGTTCGA TAAATTCCTACCTCTCTATCTATCTCTGAGATGTTTGGATTTTTCAAAACTCCATGGACATGCAGAAGAGAA ATGCTATCCACACTCGGACCAAGACAGAACTTTTACTTGTTCAAATAACAATTAAGGTGAAGCAGGGTCAG GAACGACGAATCTCTTTATGATAAACAGATCCATTTTGCAAGTTCGTTATTACGGGTAGTTCCTACAAAGG ATCGGACTAATGACGTATACAATACTTGAATTCTCGGTGTAGATGCTACATAGTTGGTTCTCATCCTTCAG AGACTACGAGTGTAATAGGAGCATCCGTCGACAAAAGGATCACCCTAAGATGATCATCTCATGGCTATTG AGAACGAATCAAATCAGATGGTTCTATTTCTCAATCTTTCTGATTTGCTCCTACGGAACCAAGATCGAAAA GATTGAAAAATAAGTCATTCACAACCACTGATGAAGGATTCCTCGAAAAGTTAAGGATTAGTAATCTTTTTT AGAAATCGAATGGATTCGGTCTTATACATACGCGAGGAAGGTAATCAAAAAAGAAAGAAGAACTCATCTTC TTTCTTTTATCACTTAGGAGCCGTGTGAGATGAAAGTCTCATGCACGGTTTTGAATGAGAGAAAGAAGTGA GGAATCCTCTTTTCGACTCTGACTCTCCCACTCCAGTCGTTGCTTTTCTTTCTGTTACTTCGAAAGTAGCT GCTTCAGCTTCAGCCACTCGAATTTTCGATATTCCTTTTTATTTCTCATCAAACGAATGGCATCTTCTTCTG GAAATCCTAGCTATTCTTAGCATGATATTGGGGAATCTCATTGCTATTACTCAAACAAGCATGAAACGTAT GCTTGCATATTCGTCCATAGGTCAAATCGGATATGTAATTATTGGAATAATTGTTGGAGACTCAAATGATG GATATGCAAGCATGATAACTTATATGCTGTTCTATATCTCCATGAATCTAGGAACTTTTGCTTGCATTGTAT TATTTGGTCTACGTACCGGAACTGATAACATTCGAGATTATGCAGGATTATACACGAAAGATCCTTTTTTG GCTCTCTCTTTAGCCCTATGTCTCTTATCCCTAGGAGGTCTTCCTCCACTAGCAGGTTTTTTCGGAAAACT CTATTTATTCTGGTGTGGATGGCAGGCAGGCCTATATTTCTTGGTTTTAATAGGACTCCTTACAAGCGTTG TTTCTATCTACTATTATCTAAAAATAATCAAGTTATTAATGATTGGACGAAACCAAGAAATAACCCCTCACG TGCGAAATTATAGAAGATCTCCTTTCAGATCAAACAATTCCATCGAATTGAGTATGATTGTATGTGTGATAG CATCTACTATACCAGGAATATCAATGAACCCGATTATTGCAATTGCTCAGGATACCCTTTTTTAGCTTCTAG GGTCTATTTCTTAGTTCAAAATCCCTCTTACTAACTGGAATCAAAGAATTAGTAGATCTGTTCCGCCCAAAA TGGGAATGGGCTAGGGTTATGAACTTATAATCTATAATCTGATGATCGAGTCGATTCCATGATTATAAGTT CATTCCATACCGGACCAGACCGGAATAGAGTTATATACATTCTCATTATGAGAAGGGGTCATTTGAGCGT ATCTAAATAGAGACTATGTTTACATATGGATCCCTACGTCGTTACATTACATTTAGGATTAGGAATAGGCG TAGTAATCGGACCTGCTTTTTACATATCTCTCGTTATTTGGGACCCTATTCACCTCTTTGGGCTTCTATTGA ATCGAGAAATGGGTTTGATTGTGTCCATCTTTTTTTTTGATAGATTGGATATCTATATAAATTGGATATCTAT ATAAGGCATTCTCCGGATAATTCAAATCGAAGCAATTGGATGTCCAACTCGGGCCTATATGACATGACCG ATCAATAGAAATACTCCACCTTTGTCATATATTCCATACATCACACTAGATAGATATCATATTCATGGAATA TGATTCACTTTCAAGATGCCTTGGTGGTGAAATGGTAGACACGCGAGACTCAAAATCTCGTGCTAAAGAG CGTGGAGGTTCGAGTCCTCTTCAAGGCATAATATTGAGAATGCTCATTGAATGGGCAATTCCATAAGAGA GCTCGGATCGAATCGGTATATCAATACCGATTCGATCCGAGCTCTTGGAATTTGAATAAGTTCGGCAGCG GATCACTAAATCTTGGTGATCTTCTCTATCTAATGAATGGGGAGTCTGCTTTAAAATCGTCCGCCCTGCAC CCAACCCACCCCCCGAGTATATGCTTCAACAGGAATCACACAGGGGTAGATTAGAAACCTCTGGTAAAAT GCCCGCCCGTAACCCAGCAGATAAAGTACATTACATAGTCCGTTTTAGGGATTGGCGATTTACCCATTCA GTGACTTTGGCACTGGACGTTCCCAAAATGGGTACTGTCGGGTCGGGTGAATTCAATAATAGACGCCTGT TGGCATTCCAGCCTTCCTTCGCTTTCAGGGCCTATCCGAAATCCAGTACTTCTTTTCTTGGTCGTGAATAT ATGAACTGGTTGTTCGCTGTTCAAGAATTCTTGTTTAGGCAGTTCATACCATCCATACATAGTGTTTTGATC TAAGATTTCAATTCTTCCGTGTTTCAGCAGTAGCATATTGTTCCATGGAGCTAAGGTCCAAAATATGGAAG AAACAAGCGTTTCCACGACTCTACCACCCAGTCAATTCTGTTCCACTTAATCCCTCTTTCATGGCCACATG TCTTTCCTGCTAAGGAATGGGAAATCTTTCTCCTGTTCCATGAATCCAATTTTCATTTCATCCGGGAAAAG CCATCTTTTTCTCAACAATGTCTTTGTCATTTGATCCAATAGCGTTCCGTTAGATAGGAATAGATTTGATAA ATACTGATAACTCTCGGATAGAGTATTAGAACGGAAAGATCCATTAGATAATGAACTATTGGTTCTAAACC ATCTCTGGCGATTAATCAACAATTCGAAGTGCTTTTCTTGCGTCTTCTTGATAAACCAGCGTTTATATATAG ATGTAGGAGGATCTGTTGGGGAAGTAAGAAGCCCCTTTGACATCTCTTCATCTGCAAATAATTCTCGATGT GAAAACACAGAGACAAAGGGCTGATCTTTGAATAGGAAAAAGAGTGGATCTGCAGGGTCCCAAATGAATT GGCTTATTCGAAAAAGGCCTTGTTCTTTGGAAGATCTATCTCGTGTCTGGTACTGCATGGTTCCACTCTGC AAGAACTCCGAATCATTCTCTTGAAGCTCATCCTCTTCATCATAAAGGATCCGCTTGCCCCGAAATGACCT GGACCAATAGGGAAATCCCAATTCATTGGGCCTTTCGATACAATCAAATAGAAAGCCCCAAGGGCGCCAT 99 ATTCTAGGAGCCCAAACTATGTGATTAAATAAATCCTCCTCTATCTGTTGCGGGTCGAGGGCTCCTTCTCC TTCCCCTTCTTCAAACTCCGATTCGTTTTTTTCATAGAGAAATCTCTGATCAAGGATAGAACAAGATCCATT TTGCATCATATCTAAGGGATTCCTTGGTTCGGGCCGAAGAAGCAATGTCACTCGATCATTATCAAACTGAC TGCAATCTTTTTCTGTCCGCGAGGATCCCACCAGAGCGCCTTCTACTTCTAATAGGCCATGAACTAGATC AGAATCATTCTCAACGAGTCCATAAGAAGTGATCCCATTTTTTTCATCGGGTCGGGGTAGAGACCAAAGA TCTTGAGCGACCGATCCGGCAGAACAACTCAAAAGATAAAGAAGTATCGTTAATTTCTTCATGCTCGTTCC AAGTTCGAAGTACCATTTGTACAAATAAGAATCCCCTTCGTTACATGATTTCTTCTTCATATAGATAGATAT AGGATCTATGGGGCAATTACTTAGAAGTACATTTTGTGCTACAGCCCTTCCTATCTGATAGAAAAGGATCC CATGATCCTGAACCGATCTTACCTGGGATCGCAAATCCCAAGTTTGTCTATGAAGAGCGGATCTAATTGTA TTAGTGTCTATAATTGATTTCTTCTGTGTAATACTAATCGATAGGGCCTCATTGGTAAGTGCTACAAGATCT CGTGCATTGGAACCCATGGTTATGGACCCGAATCCATTAGTATGGAACATTTTATTTTCCAAGCGAAATCC CCTAGTATATGAAAGGGTGAAAAAGTGCTTTCGTTGTTGTGGAATAAGAAGCCTTCGTATCTTAATGCACG TATTTAATTTATTCGGAGCTATTAGAGCGGGATCCACTTTTTGGGGAATATGAGTCGAAGCAATAACAAGA ATATTTCTAGTGGAACATCTTTCACAATCCCTGGAGAGATGGTTCACTAATAGACCGAGGGAGAAGTAATT CGACTCATTCACATCCAGATCATGAATGTTTGGAATCCATATTATGCAAGGAGACATTGCTTTTGCTAATT CGAATTGAAGGGTGATATAAAATCGGTCTATTTCCGGCATCATATCCATAGTTAGCAGTTCCAGCTCCGTA TGAAGGTCACGATCGATATCGTCACTAGCATCAATATCGTCACTAGCATCAATATCGTCACTAGCATCAAT ATCGATATCATCAATAAGAAAACCTTTAGGCTTGTTATCCAGGAACTTGTTCAGAAATACCGTAATGAAAG GAACATAGGAGTTTGTCGCTAGGTATTTGACCAAATAGGATCGTCCAGTTCCTATAGAACCTATCACTAAA ATACCCCTAGAGGGGGATAAGGCTAAGCGGAGCGAAAAGGGTTTTCCATGAGATGGGAAATTCAAACTA TTAGCCCCACACGAGGTTTGTGAATAAGTGATTGTCTGATAATGAGCAAGGAATATCCGTCTTTCTGCTAA ACAGGATGTATTGAACTCATAATTCATTAGATACTTTTTATGAATGTCAACTAAGTATCGTAAGTAAATTGC TCCCGGTTGTTCAATCATTTGATAGCCAGAGTCATTCTTTGATAAATGATCACTATGAGTCAGACTCAATA GAATTTGATCAATCCTTTTTTCTGTCGTTAAGGTGGAGAACTGAACCAAGAATTCTCTTTCTTCATCATCAA TCGAATCACTGTTTGCGACCCAGGATTCTATTTTATCATCAATCCAATCCCCGTTCACATTTTTTCTTTTTC TTATCAATGAATAGATCTCTTTACTTGTATGACTTAGATGTCTCGTATTTCTCGAAAAAGTGATTCGATTGA TGGGATTTGGTATGATACTTATGAGATCGATGATATCGATGAGATTGATATTCAAATATTTCTTCTTAGAAC GTATTGATTTGACCCCATAAGCGGGATCACCACCCAATAGCATGTTGCCGCCAGAAGCAGAACCCCGTAT TTCTTCTAGAGAATCTCCTAATTGTTCCAGAGCAACTAGAAAGAGATTCTTTAACCAGAAAGAATTCGGTT CAGATGTAGGATACCTATCCAGAAGTTTTCGCAACTCAATCATAGATGATGGAATCATCAAAGATTTGACC TTTTCAAACTCTGTCTGTAACTCACTAGAGGCCCAGGAAACAAAGAGAAGATGTGTACAAACGAGATATC CAGCAACAAGAAGAAGGAAAAGGATTGAATAGAGGAACTCCCGAACATTTGGCGATCTCAGATGTGTCGA TATCAATGGCGACTCATTATTTCGATGAATCCTTTCTTCGGACAGAAGAAGATTATGGAAACACTTACTCG AAATCTCACTTATCAGATTCCATTTCCATTGTAGAAGACACAATTTTTTCTGAAGAATTCGCCATGATATAT CTGATCCATGCATAATATCATGAAAAATGGATACAAATTTTTGACTGCTACTTAGACTTAGTATCGGCAATA GGTCTGAAAAAGTATCTAAAAATATAAAATTTAGATATTTGTACCCTGTCGAAGTAAGGAACCATGGCATAT ATGTTTGGAATAGATTCCATTTTGAGAGAGTTGAAAAAGCACTATCTCGTTGAAAGGTTCTATACATCTGC CCTTTCTCAACGCATTTCTTTAGACAAAGACTCCGTTTTTTCCTCTTTTCGGATGGTAAATATTTCTCAGAA CATGGAGTGTGAATCAAACCCATGTTTGAATTGAAATTGAGATACTGATGCGAGTTCTTCCCTTCTGAATC AGATAGATTCATATCTGAAAGAGGTTGACAATAAGTTCTTTCAAAATTGACTATTTGTCCCTCTGTTAAAGG TGTTCCAGAAATGTCTGCGATCGAGTAAATAGCTCTACGAACGAATGGGTCGGATCGACTTGGAAAATGG AAAGATTTGTACAAGTTATACGTTTCGTCACCACCACTTTGTGGAAAATCGTTAGGTATGAATATGTTAGAT ACCTGTGACTCGATTGGTGAAATAGTATCTCTCCCCCAAAAAGCATGTTTTTTTTTACCGACGCACAAAGA AAAAATTTTGTTGTGAATGAACAAGATATTGAGGAATTGTCCATACGTAAAATCAGAATTATTGATACGGG CCTTTTCCACATAAAAAGGGAATCTTTTATTACAATAGAAGCAGAAGTGATGTGGATTATTCAAGAATCGA AGTCGATTTGCTTTATAAAAAGAAGATATCAATGAACTTCTATGAAATGGTTTCACGGGATTCAGCCAATT GTCTTGATCGTGGAATATCGTTGAGAAATAGGAATCCGTGTTATCAAAGGATTTCCTGCGATTATTTCTAG TATGGAATGAGTCAATCATCCACTTTGGTATCTTATTGAACAAAAATGGTGATATTGTTCCTCCATTGATCA AGAATTTCGATTTTTGGGAAGTATCATGATCATCCAATAAATCCAATAAGAAGGGTTTCCATTTTTTCAAAT GAACGATTTGAAGACCTATTGATTCTAACAACTGATTGCAGAGTTGATCATTCGGACCTTTCAATTCATAG ATGTAGATCTCGGACCTATGAATGGGGATATTTCCGAAACTCACAAAGAAAAAAGGAAGTGAGTTAGACA AAAAGAAAAGCAACTTGGACAAAAAGAAACGAAATGGCTTAGACAAACCTTTTTTGTCGATAACCTCAGAC CAATCAATCGAATATTGATTAATACGTAATCGATCGAACACTACTTGAAAACGGCTCTTCTGCTCAGAAAC GAAATGTTCCAAATGTTCCTGGAAATTCTTGCTCCCATTGGACCATTTGTATCTATATGCATCAGGATCCC GATTCATGGATCTCTCGGTTCGAGAAATAAAAATAAGAGGATCGAACCATCTCTTCTGACTCTTTTTCAAA TTCGAGAAATGTTGGTTGATCGTATATTTCATTATAGTTCTATGATTCAGAGTATCCTTTCCTATTTGATCC CTTTGAATTCCATATTCGAAGTTGCGATCGGATCTATTCATTAAAAAGAATCGATTCAATACATTTCTTATG TACCCATAGGTGCTATATTGGATTTGAATCAGATTTCGGATCAATCTATATTGATTGACTGCCTCCATTATG 100 TTGTTGCTAGCAAATACCACTATTTTTGGTTTTGGATCTTCCAAATCATTCCCGCAGGAGATCCGGACCCA TTTTTTTCTGATCCTTCGATAAAAAGATTCATTCTCTTCATAAAAAAAAGGAGGTAGAGCCAATAAAGATTT TTTTTTCGATTCATCCCTGGAGTCGAATACCTCATTCAAGAATTGTTTTTGATCCAATCCGTAGGAATCAAT AGAAAAGGCAAATCCCTTATGATACACCAGATCCGGCTCGGTTATTGATAGAGTGAATAAATCTGCCATTT CTTGAAATCTCTCTTCTGATTCAAAATCGTGGTGTAACGTGTATCCTCCCCTGTTCCGGTCATGGAATAGA TGAAATAAATCAAAAAATGGATTTTTGTTCAAGAATGAAATCTTATTGGAACTATCCATATCCGGTTCATCC TTCGGAACCATATCACATCCCGGATATGATGAAATAGGATGAATTGAGACGGTATTTTGTAAATACGTAAT TATCTTGAATATATTAACCATTTCTTTCTTTTCCGATCGCCTCGAAGGGACAAAAGAAAGATCTTGTTGTTT CTTCAACAATTTCTGATCTCTAGTGGACCTCTCAGTAGGATTCGAACCCAGATGAAGTTCTGACCATCTGT CAGAGAAAAAAGAACGAACGGATCTTGTAGGATTCCCAAGAAATTCTTCGATTTCTTCCGGAAGCAGATG ATTAATCATCTGCTTCTCACGTTCCGTGAATAGCCGGGACATTGAGGAATATACAGAAAGGCATTTCGGG AATCGGTCTGATTCTATCTCTGTTCGTTCCGTTTGAAGAAAGGAAGGATCCCAAAGAATCGATCTTTCTTT TAGTTGTTGAATCTCTCTTTGATTGATCAATGTGTGATATTCCGAATCCTCATTACTAATGGAATCTAAAGG ATCTCTGGATTGATCAGAAGATCCTTTCAATTGGCTAGAATCCCTCTTTTTTCCGATCCAGTTCCTCCACC ACCGCGAACCCCAGTTAGATTCAGGCATGCTACACTTTTTAGTTATTGGGAGAACCCAAGTACTCTCTTTC GGATTCAGGAAACAACTCTCAGAGATCTTTTTTCCTTTTGGAAGATACAGGAGCGAAACAATCAACCTATT GATATTGGAAGACCCAACGGATTCTTCCAATGTATCATTTCTGGGTCCAATGGAATTCATAGGTATAGGAA GAAGCCCTATCAAATAGAGATTTTTTCTTTCGACCATATTTCGACTGTTAATACGATATATAAGGACCGCTA CTACAAAGAGTATTACACCCTTGATCGTGAAATATCGATTGCTTGTTGAACCCTGTGAATTGCGTGAAAGT AGGATACTCCAAATTCGGGGGTCAAAAAGTTTTATAAAACGTTCTTGGTGGAAAAAAATGTGACTGAAAGA TCCCACTGAATTGAATTGGGTCCGTGAATCTAAGAAATAGTGAGAATTCTTGATCTCTCTCAATATCTCTCT CAATTCGAAAATCCAGGATTTGAATTGATGTCCTTTCATTGATTCCTCCTAAATTGCATTGATTTATTCTAAA GATTTCGTTTCAATTGGAATTTGGTTATTCACCATGTACGAGGATCCCCGCTAAGCATCCATGGCTGAATG GTTAAAGCGCCCAACTCATAATTGGCGAATTCGTAGGTTCAATTCCTACTGGATGCACGCCAATGGGACC CTCCAATAAGTCTATTGGAATTGGCTCTGTATCAATGGAATCTCATCATCCATACAAAACGAATTGGTGTG GTATATTCATATCATAACATAATATATGAACAGTAAGAACTAGCATTCTTATTGAGACTAGAACTCATAGGG AAGAAAATCGATTTATGGATGGAATCAAATATGCAGTATTTACAGACAAAAGTATTCGGTTATTGGGGAAA AATCAATATACTTCTAATGTCGAATCAGGATCAACTAGGACAGAAATAAAGCATTGGGTCGAACTCTTCTT TGGTGTCAAGGTAATAGCTATGAATAGTCATCGACTTCCGGGAAGGGGTAGAAGAATGGGACCTATTATG GGACAGACAATGCATTACAGACGTATGATCATTACGCTTCAACCGGGTTATTCTATTCCACCTCTTAGAAA GAAAAGAACTTAAATCAAAATACTTAATAGCATGGCGATACATTTATACAAAACTTCTACCCCGAGCACAC GCAATCGAGCCGTAGACAGTCAAGTGAAATCCAATCCACGAAATAATTTGATCTATGGACAGCATCATTGT GGTAAAGGTCGTAATGCCAGAGGAATCATTACCGCAGGGCATAGAGGGGGAGGTCATAAGCGTCTATAC CGTAAAATCGATTTTCGACGGAATGAAAAAGACATATATGGTAGAATCGTAACCATAGAATACGACCCTAA TCGAAATGCATACATTTGTCTCATACACTATGGGGATGGTGAGAAGAGATATATTTTACATCCCAGAGGG GCTATAATTGGAGATACCATTGTTTCTGGTACAGAAGTTCCTATAAAAATGGGAAATGCCCTACCTTTGAG TGCGGTTTGAACTATTGATTTACGTAATTGGAAGTAACCAATTAGGTTTACGACGAAACCTAGAAATCGAT CACTGATCCAATTTGAGTACCTCTACAGGATAGACCTCAACAGAAAACTGAAGAGTAACGGCAGCAAGTG ATTGAGTTCAGTAGTTCCTCATATAAAATTATTGACTCTAGAGATATAGTAATATGGAGAAGACAAAATTGT TTCAAGCACCGACAGAACCGGAAGCGCCCCTTCTTTCAAAGAGAAGAGGACGGGTTATTCACATTTCATT TGATGGTCAGAGGCGAATTGAAAGCTAAGCAGTGGTAATTCTAAAGATTCCCCGGGGGAAAAATAGAGAT GTCTCCTACGTTACCCATAATATGTGGAAGTATCGACGTAATTTCATAGAGTCATTCGGTCTGAATGCTAC ATGAAGAACATAAGCCAGATGACGGAACGGGAAGACCTAGGATGTAGAAGATCATAACATGAGTGATTCG GCAGATTTGGATTCCTATATATCCACCCATGTGGTACTTCATTGTAGGATATATAAGATCCATCTGTATAGA TATCATCTACATCCAGAAAGCCGTATGCTTTGGAAGAAGCTTGTACAGTTTGGGAAGGGGTTTTGATTGAT CAAAAAGAAGAATCTACTTCAACCGATATGCCCTTAGGCACGGCCATACATAACATAGAAATCACACGTG GAAAGGGTGGACAATTAGCTAGAGCAGCGGGTGCTGTAGCGAAACTGATTGCAAAAGAGGGGAAATCGG CCACATTAAAATTACCTTCTGGGGAGGTCCGTTTGATATCCAAAAACTGCTCAGCAACGGTCGGACAAGT GGGGAATGTTGGGGTGAACCAGAAAAGTTTGGGTAGAGCCGGATCTAAGCGTTGGCTAGGTAAGCGTCC TGTAGTAAGAGGAGTAGTTATGAACCCTGTAGACCATCCCCATGGGGGTGGTGAAGGGAGGGCCCCAAT TGGGAGAAAAAAACCCACAACCCCTTGGGGTTATCCTGCACTTGGGAGAAGAAGTAGAAAAAGGAATAAA TATAGTGATAATTTTATTCTTCGTCGCCGTAGTAAATAGGAGAGAAAATAGAATTTATTTCTTCGTCTTTAC AAAAAAAATAGGAGTAAGCTGTGACACGTTCACTAAAAAAAAATCCTTTTGTAGCAAATCGTTTATTAAGA Trình tự cặp mồi sử dụng kiểm chứng/ hiệu chỉnh trình tự hệ gen lục lạp sâm Ngọc Linh 101 Vị trí Cặp mồi 870 2560 5130 35710 95379 110818 115160 116080 134110 136610 1-P530 1-P1070 2-P2374 2-P2900 3-P4820 4-P5790 5-P35319 5-P35890 9-P99550-F 9-P100201 10-P112220 10-P112680 11-P114770 11-P115574 12-P115730 12-P116500 14-P133950 14-P134220 15-P136200 15-P136910 Danh sách vị trí gen đƣợc giải trình tự DNA lục lạp sâm Ngọc Linh 102 103 ... 1.3 .Giải trình tự gen hệ gen lồi thuộ 1.3.1 Giải trình tự gen phục vụ định loại phân tử 1.3.2 Giải trình tự hệ gen nhân hệ gen biểu 1.3.3 Giải trình tự hệ gen lục lạp ... methyl hóa CpG 34 3.2 Giải trình tự hệ gen lục lạp sâm Ngọc Linh 36 3.3 Kết lắp ráp gen hệ gen lục lạp sâm Ngọc Linh 38 3.4 Kết hiệu chỉnh/ kiểm chứng trình tự hệ gen lục. .. trình tự hệ gen lục lạp sâm Ngọc Linh 39 3.5 Kết phân tích giải hệ gen lục lạp sâm Ngọc Linh .41 3.6 Đề xuất thị phân tử tiềm khai thác từ trình tự hệ gen lục lạp sâm Ngọc Linh 45