The thesis evaluated the safety and improvement of spermatogenesis of Y10 capsules in experimental animals; Effect of improving the fertility of capsules Y10 in patients with impaired sperm.
o excrete inhibin, under inhibitory effect of inhibin B the amount of FSH will excrete from the pituitary gland decreases In addition, Y10 can act directly on the hypothalamus – pituitary testes to regulate the activities of these endocrine glands. Therefore, patients, whose LH, FSH and testosteron levels were within the physiological limits, either increased or decreased within the normal physiological limits. Thus Y10 has the effect of stimulating the body to increase serum testosterone production and regulating LH and FSH secretion Y10 did not increase semen volume of each ejaculation, did not change the physiological resolution of semen, it reduced the number of white blood cells Semen indexes such as sperm density, alive sperm rate, progressive sperms, rapid progressive sperms, and the proportion of normalshaped sperms after the treatment all increased compared to before the treatment In particular, Y10 increased the rate of rapid progressive sperms, which was a healthy sperm, capable of moving deep into the female reproductive tract and is one of the important indicators of sperm that plays a decisive role in the conception process ensuring the creation of a completely healthy embryo This result is completely consistent with the experimental results: in white rats, Y10 increased the number and proportion of progressive sperms, increased the proportion of normal shape sperms; In rabbit testicular histology, Y10 made sperm cell proliferation, had sufficient and balanced stages, the number of spermatozoa in the OST was much higher than the control group The quantity and quality of sperms increased because Y10 increased the endogenous testosterone secretion, Y10 had the effect of regulating the secretion of LH and FSH Cornu cervi parvum and Cordyceps militaris contain "amino acids, fats, sugars, vitamin, etc." which are essential for the proliferation of spermatic epithelium and sperm. Cornu cervi parvum and Cordyceps militaris consists of amino acids, vitamins, and trace elements. These substances in Y10 work well together and since it has a cellular antioxidant effect, including sperm cell lines and reproductive endocrine glands, Y10 increases the number and the percentage of alive sperms, increase sperm motility, increases the proportion of sperms with normal shape after the treatment Our study of 30 patients diagnosed with progressive kidney failure after 2 months of treatment, all clinical symptoms due to the deficiency of kidney and Jing significantly improved compared to before the treatment. This can be explained by the composition of Y10 from Cornu cervi parvum and Cordyceps militaris According to traditional medicine, Cordyceps militaris has the effect of warming kidneys and producing Jing, used to treat impotence and deficiency of kidney Yang. Cornu cervi parvum has effects of tonifying kidneys and nourishing blood Jing, when they are used for long they will be beneficial to both Yin and Yang Jing. The combination helps to regulate yin and yang, the essential aura is fostered to make the kidneys welloff, the yin and yang balance. The preparation process confirmed by pharmacological studies showed Y10’s therapeutic effects * Undesirable effects The treatment 30 patients Y10 in months showed no undesirable effects such as vomiting, loose stool or conspitation, and rashes CONCLUSION 1. The safety of Y10 on experimental animals Acute oral toxicity on white mice: The LD50 of was not found in the mice The highest possible dose for mice to drink, 20.0g of high medicinal herbs / kg body weight (equivalent to 25.0 g of powder in capsules / kg body weight), caused death, no acute toxicity Semichronic toxicity on white rats: The experiment on the white rats group taking Y10 capsules at 224mg decoction / kg / day and 672mg decoction / kg / day showed: The rat were healthy, gained good weight, there was no influence of the medication on the rat heart waves. The medication did not change the hematological indexes (erythrocytes, hemoglobin, hematocrit, average volume of red blood cells, white blood cells, and platelets). It did not change the biochemical criteria to assess liver and kidney function (activity of enzymes AST, ALT in blood, Albumin plasma, total bilirubin, total cholesterol in blood, Creatinine in blood). It caused no damages to histopathology of liver, spleens, and kidneys Reproductive toxicity: Y10 384mg decoction / kg / day and 1152mg decoction / kg / day in 60 days did not affect the normal development of the fetus and offspring born to P and F1 generation Chromosomal toxicity: There was no difference in the rate of occurrence of number of infection chromosomes disorders (polyploid, polyploid), chromosomal structural disorders; frequency of different types of chromosome mutations in testes of white mice taking Y10 384mg decoction / kg / day and 1152mg decoction / kg / day in 28 days from that of the control group p> 0.05 No chromosomal structural disorders and chromosomal cluster disorders were found 2. The effects of improving fertility of Y10 On experimental animals The male white rats that caused sperm deficiency with Natri valproate, Y10 224 mg of medicinal herbs / kg / day and 448 mg of medicinal herbs / kg / day in 6 weeks had the same indicators (serum testosterol concentration, sperm quantity and quality, weight of sexual organs and testicular histology) as the control group. This effect is equivalent to the testosteron 16mg / kg / day On patients with sperm decline Y10 4 capsules / day, in 2 months, is safe and effective in treating patients with sperm decline increases the concentration of serum testosterone, regulates the secretion of LH and FSH, and increases the sperm count and quality The ‘very good clinical treatment results’ (the semen increases to normal) is 16.67%; Good (increase in sperm count and quality c) is 66.67%; The average (increases the either the sperm count or quality) is16.67%; No result is 0% improves symptoms of the disease according to traditional medicine does not cause any undesirable effects; does not alter blood biochemical indices; does not change the hematological index RECOMMENDATIONS 1. It is possible to conduct more research to assess the effect of the medication on other types of sperm decline according to traditional medicine, as well as other pharmacological effects such as antioxidant, liver function protection, enhancement immune system, cardiovascular effects, etc. to prove the therapeutic of Y10 2. It is advisable to massively produce the medication due to its effects proven in the study. ... of sperms increased because? ?Y10? ?increased? ?the? ?endogenous testosterone secretion, Y10? ?had? ?the? ?effect? ?of? ?regulating? ?the? ?secretion? ?of? ?LH? ?and? ?FSH Cornu cervi parvum? ?and? ?Cordyceps militaris contain "amino acids, fats, ... capable of moving deep into the female reproductive tract? ?and? ?is one? ?of? ?the? ?important indicators? ?of? ?sperm that plays a decisive role in? ?the? ?conception process ensuring? ?the? ?creation? ?of? ?a ... treatment 30 patients Y10 in months showed no undesirable effects such as vomiting, loose stool or conspitation,? ?and? ?rashes CONCLUSION 1.? ?The? ?safety? ?of? ?Y10? ?on? ?experimental animals