Đánh giá sự ô nhiễm kim loại nặng trong thuốc đông dược và nghiên cứu đánh giá gốc và phân bố kim loại nặng trong một số cây thuốc

DAI HOC QUOC GIA HA N Q I TRUONG DAI HOC KHOA HOC TUNHIEN BAG GAG TGNG KET DE TAI DANH CIA SU NHIEM KIM LGAI NANG TRGNG THUGC OGNG DUQC VA INGHIEN CUU OAINH GIA NGUGN GOC VA SU PHAN BO KIM LGAI NANG TRGNG MGT SG CAY THUGC Mas6: Chii tri dk tai: Cac can bo tham gia: QG-09-13 PGS.TS Ta Thi Thao Ths Nguyen Ngoc Son ThS Nguyen Thi Van Anh CN Nguyin Van ThuSn HANOI-2011 MyCLUC Trang Bao c^o tom tat ket qua thuc hien de tai Project's Summary Mo dau J Chucmg 1: Tong quan tai lieu 1.1 Tinh hinh bao che, sir dung dugc lieu va nhilm doc kim loai nang dugc lieu a Viet Nam hien 1.2 Cac phuong phap phan huy mau xac dinh lugng vet kim loai nang mau dat va mau thuc vat l,2.1.C^c phuang phap phan huy mau xac dinh lugng vet kim loai nang mau dat, tram tich 1.2.2 Cac phuang phap phan huy mau xac dinh lugng vet kim loai nang mau thuc vat 1.3 Phuong phap khoi plasma cao tan cam ung (ICP-MS) xac dinh lugng vet kim loai nSng 1.4 Phan tich thong ke da bien xac dinh nguon goc va su phan bo kim loai nSng 1.4.1 Khai niem phuang phap PCA va CA f ^ r \ f r n ^ Q IQ ^2 j^ fir 1.4.2 Mot so thong ke da bien xac dinh nguon goc chat nhiem 14 Chuang 2: Thuc nghiem jy 2.1 Dung cu va hoa chat jy 2.2 Lay mau va bao quan mau 19 2.2.1 Mau dugc lieu va thang thuoc dong y 1^ 2.2.2 Lay mau dat cay thuoc 24 2.2.3 Mau cay thuoc 26 2.3.Quy trinh xu ly mau dat va mau cay thuoc xac dinh kim loai nang 28 2.3.1 Quy trinh xu ly mau dat xac dinh kim loai nang 29 2.3.2 Quy trinh xu ly mSu cay thuoc va thang thuoc dong y 29 2.4.Phuang phap phan tich va xu ly so lieu phan tich 29 Chuang 3: Ket qua nghien cuu va thao luan 33 3.1 Nghien cuu t6i uu hoa cac di§u kien phan tich cua phuang phap ICP-MS 33 3.1.1 Chgn dong vi phan tich 33 3.1.2 Do sau mau 34 3.1.3 Cong suat cao tan 34 3.1.4 Luu lugng mang 34 3.1.5 Tom tat cac thong so toi uu 3.2 Danh gia phuang phap phan tich ICP-MS 3.2.1 Khoang tuyen tinh 3.2.2 Duong chuan xac dinh ham lugng 11 kim loai 3.2.3 Giai han phat hien va giai han dinh lugng 3.2.4 Danh gia chinh xac (do dung va chum) cua phep ICP-MS 3.3.Nghien cuu xay dung va danh gia quy trinh xu ly mau 3.3.1 Xay dung quy trinh xu ly mau thuoc dong y 3.3.1.1 Cac quy trinh xii ly mau khao sat 3.3.1.2.Danh gia hieu suat thu hoi cac qui trinh xu ly mau cay 3.3.1.3 Do chinh xac ciia phuong phap phan tich mau thuc te 3.3.1.4 Danh gia hieu suat thu hoi 11 kim loai nang vai quy trinh xu ly mau cay lua chgn 3.3.2 Lua chgn qui trinh xu ly mau dat 35 36 36 36 37 40 42 42 42 44 .« q 3.3.2.1.Danh gia hieu suat thu hoi r 3.3.22 Danh gia chum (qua lap lai ) ^I 3.3.3 Danh gia phuang phap phan tich thong qua mau chuan dugc chung nhan CRM 3.4 Ket qua phan tich mau thuc te 3.4.1 Ket qua phan tich ham lugng kim loai nang dugc lieu va thuoc dong y 3.4.2 Ket qua phan tich ham lugng kim loai nang mau cay thuoc va mau dat cay thuoc 3.5 Danh gia ham lugng kim loai nang dat va cay 53 c-^ ^^ ^Q 3.5.1 Danh gia ham lugng kim loai nang a dat ^-Q 3.5.2 Su phan bo ham lugng kim loai nang mot so cay thuoc ^^ 3.6 Buac dau khao sat su phat tan kim loai nang tu dugc lieu vao thuoc sac 3.7 Danh gia su tich luy kim loai nang dat va cay 3.7.1: ich luy kim loai nang theo thai gian thu hoach cay thuoc: ^^ 68 3.7.2 Phan bo ham lugng kim loai dat theo sau 68 ^g 3.7.3 rich luy kim loai cac bg phan cua cay thuoc ^Q 3.7.4 MANOVA danh gia anh huong ciia dia diem, thai gian thu hoach cay thuoc den ham luaim KL tronii cav thuoc 3.7.5 He s6 tich luy sinh hoc cac kim loai nang tCr dat len ca\ thuoc JQ 3.8 Phan tich thdng ke da bi^n danh gia nguon goc va sir phan bo nhiem kim loai nang mau dat ca\ thuoc va mot so cay thuoc dong dugc -j^ 3.8.1 Phan tich cau tur chinh (PCA) ^ ^ 3.8.2 Phan tich nh6m(CA) 73 76 DANH MyC CAC CHU VIET TAT AAS Phuang phap hap thu nguyen tir ANOVA Phan tich phucmg sai CA Phuang phap phan tich nhom CRM Mau vat lieu chuan dugc chung nhan CV-AAS Pho hap thu nguyen tii' diing ky thuat hoa hai lanh F-AAS Pho hap thu nguyen tu' nggn lua HSTLSH He so tich luy sinh hoc HVG-AAS Pho hap thu nguyen tii' dung ky thuat hidrua hoa ICP-AES Phuong phap phat xa nguyen tu- cao tan plasma cam ung ICP-MS Phuang phap khoi cao tan plamsa cam ung KLN Kim loai nang LOD Giai han phat hien LOO Giai han dinh lugng MANOVA Phan tich dong phuong sai PC Thanh phan chinh PCA Phuang phap phan tich phan chinh TB Trun" hinh BA O CAO TOM TAT KET QUA THlTC HIEN De t^i nghien ciru dac biet cSp Dai hoc Qu6c Gia Ten de tai: D^nh gia su' nhiem kim loai nang thuoc dong du'oc va nghien ciru d^nh gi^ nguon goc va su* phan bo kim ioai nang mot so cay thudc Ma s6: QG-09-13 Chu tri da tai: PGS.TS Ta Thi Thao Don vi cong tac: Khoa Hoa hgc- Truong Dai hgc Khoa hgc tu nhien Dia chi: 334- Nguyen Trai- Thanh Xuan- Ha Ngi S6 dien thoai: 84-4- 858.4287 So can bg tham gia: 03 Ths Nguyin Ngoc So*n- Khoa H6a hoc - Truong DHKHTN- DHQGHN ThS Nguyen Thj Van Anh- Hgc vien nghien cuu y Dugc co truyen Viet Nam m, r CN Nguyen Van Thuan- Hgc vien cao hgc K18 IMuc tieu va noi dung nghien cuu - De tai nghien cuu nham xay dung qui trinh phan tich, danh gia muc nhiem cac kim loai nang mot so loai thuoc dong dugc dang luu hanh tren thi truong Viet Nam hien nay, dong gop vao viec kiem soat chat lugng thuoc dong dugc hien dang dugc su dung rgng rai duai dang thuoc bo hoac thuoc dieu tri Ben canh viec danh gia sir phat tan kim loai nang vao thuoc uong sau sac se la co so ban dau da xay dung tieu chuan ham lugng kim loai nang thuoc dong dugc Cac ket qua danh gia ngu6n g6c ciia nhiem kim loai nang se dugc tien hanh tren doi tugng dat trdng nhieu vung khac va Ian truyen nhiem tu dat vao cac bo phan ciia cay dung lam thudc nhu re, than, la, qua, hat va danh gia Ian truyen du lugng kim loai nSng theo thai gian da xay dung khuyan cao va lugng kim loai nang co mat thudc dong dugc dung lam nguyan lieu dua tran diau kien tho nhuong, trong, cham soc v^ thu h^i, bao quan - De tai g6m nhijng ngi dung nghian cuu chii yau sau: f t , +T6i uu hoa cac diau kien phan tich dong thoi lugng vat va siau vat cac kim loai nSngnhuCu, Pb, Cd, Zn, Co, Ni, Cr, Mn bSngthiatbi phan tich ngudn cao tAn cam ung plasma- khoi (ICP-MS) co danh gia ham lugng hai nguyan t6 hay gay sai so phan tich bang phuang phap la As va Hg da rut co c4n thiat phai phan tich chung tren thiat bi hap thu nguyan tu, ap dung ky thuat hidrua hoa ( HVG- AAS) hoac hoa hoi lanh (CV-AAS) hay khong -fNghian cuu xay dung cac qui trinh xu ly mau thuoc dong dugc va xu ly mau dat + Danh gia su Ian truyan nhiam kim loai nang vao cay thudc dilu kien th6 nhuong, va cham soc, thu hai bao quan -I- Dung phan tich thong ka da bian danh gia nguon goc phat tan kim loai nang thuoc dong dugc trgt Cac ket qua dat duoc: 3.1 Ket qua l mau bang binh Kendal va qu\ trinh su dung 16 vi song va lira chgn dugc quy trinh xu ly mau phu hgp voi trang bi hien co la phuang phap kho u6l ket hgp hoac xu ly 16 \ i song dat hieu suat thu hoi tu 90-95% va lap t6t CV dugc thu thap tren dia ban quan Hoan Kiem va Ha Dong (Ha Ngi) Ket qua phan tich cho tha\ chua phat hien thay sir nhiem doc kim loai nang cac mau nghien cuu Nghien ciiu cung da buac dau danh gia su phat tan kim loai nang tu dugc lieu vao thuoc sac Su phat tiin thuoc vao tirng kim loai, mot so kim loai phat tan tuong doi nhieu (tran 30%) nhu Ni, Cu, Zn Ket qua phan tich ham lugng 1 kim loai nang dat cay thu6c va mot s6 ca\ thuoc tai hai dia diem la \ ucm thuoc cung cho thay c6 sir cao bk th\ibng mot s6 kim loai Viec sir dung chain th6ng ke Student dl danh gia cung cho thay co sir khac co nghTa v^ ham lugng trung binh m6i kim loai tai hai dia diem khac nhau, theo thai gian sinh song ciia cay va kha nang tich luy sinh hgc ciia cay theo tuoi * Dua tran kat qua dat dugc, bang phan tich thong ka da bian da danh gia dugc nguon goc phat tan kim loai nang cay thuoc dong dugc tran dAt nghian CUU, tu riit dugc nhan xat va diau kien tho nhuong va cham soc, ban chat tu nhian ciia chat dat gay sir khac c6 nghTa va ham lugng cua mot so kim loai nang khac Ngoai ra, muc tich luy kim loai nang khac voi timg loai cay va theo thai gian khac cung gay bian doi khac ve ham lugng trung binh Vi vay, can co nhung nghian cuu cu tha hon voi tung loai cay thuoc da danh gia muc anh huong cua kim loai nang tu co tha dua kat luan chinh xac 3.2 Ket qu^ ling dung: - Qui trinh phan tich dong thoi tong ham lugng 11 kim loai nang Cr, Mn, Fe, Co, Ni, Mn, Cu, Pb, Cd, Zn, Pb, As va Hg bang phuang phap ICP-MS voi nhay va chinh xac cao da dugc ung dung da phan tich ham lugng cac kim loai nang dat, cay thuoc va thang thuoc dong dugc cung nhu thuoc sac tran thiat bi ICP-MS tai khoa Hoa hgc- Truong Dai hgc Khoa hgc Tu nhian- Dai hgc Quoc Gia Ha Ngi - Da nghian cuu v^ ling dung phuong phap thong ka da bien da phan tich tap so lieu va ham lugng 11 kim loai nang cay thuoc va dat cay thuoc tai hai dia diam khac da kat luan \e phan bo ham lugng kim loai nang va nguon goc phat tan kim loai nang vao cay thuoc gop phan on dinh va kiam soat chat lugng dugc lieu tu goc So lieu thu dugc cung minh hga tot dang bai giang va phuong ph^p thong ka da bian chuyan da bat bugc day cho sinh vian cao hgc chuyan nganh Hoa phan tich cua khoa Hoa 33 Ket qu^ cong bo - Ba bai bao khoa hgc da dang tran tap chi khoa hgc chuyan nganh : Ta Thi Thao, Nguyen Thi Van Anh, Nguyen Nggc Son Nghian cuu danh gia ham lugng kim loai nang dong dugc bang phuang phap khoi plasma cam ung (ICP-MS) Tap chi khoa hoc va cong nghe Tap 48, s6 2A, (2010), trang 469477 Ta Thi Thao, Nguyin Van Thu4n, Nguyen Thi Huang Tham Nghien cuu danh gia nguon goc va phan bo kim loai nang mot s6 cay thudc va dit trdng cay thuoc bang phuang phap phan tich ICP-MS va th6ng ke da biln Tgp chi Hoa hoc T 48, 4C, (2010), trang 396-401 Ta Thi Thao, Nguyan Van Thuan Ung dung phuang phap khoi cao tan plasma cam ung (ICP-MS) da phan tich danh gia ham lugng kim loai nang mot SO cay thuoc va dat cay thuoc Tap chi Phan tich Hoa, Ly va Sinh hgc Viet Nam Tap 15, S6 (2010), trang 223-229 3.4 Ket qua dao tao 3.4.1 Cii' nhan: 02 - Nguyen Thj Thanh Dao Nghian cuu danh gia ham lugng asen va thuy ngan mot loai cay thuoc va phat tan vao thuoc sac Khoa luan tot nghiep- Khoa Hoa hgc- Truong DH KHTN, 2009 - Nguyen Thj Hong Phan tich ham lugng mot so kim loai nang thuoc dong dugc bang phuang phap hap thu nguyen tu Khoa luan tot nghiep- Khoa Hoa hgc- Trudng DH KHTN, 2011 3.4.2 Thac sT: 01 Nguyen Van Thuan Nghian ciiu danh gia nguon goc va su phan bo kim loai nang mot so cay thuoc.Truong Dai hoc Khoa hgc Tu nhien DHQG Ha Ngi, 2010 3.4.3 Ty dao tao: Nang cao tay ngha phan tich va xay dung cac qui trinh phan tich tran kim loai nang doi tugng dat, tram tich va thuc vat tran thiat bi phan tich hiendai ICP- MS Tinh hinh kinh phi ciia de tai: 100 trieu dong (mot tram trieu dong) 4.1 Thua khoan chuyen mon: 42.000.000 d -Chi phi nghiep vu chuyan mon: 54.000.000 d 4.2 Chi mua sam trang thiet bi: 4.3 Chi toan dich vu cong cong: 4.000.000 d Danh gia tinh thinh thuc hien nhiem vu nghien cuu: da hoan nhiem vu dugc giao Ha Noi, 24 thang nam 2011 Chii nhiem de tai Khoa quan ly V ?(^^^s, ^ t ^ - ^ ' ^^^ PCS TS Ta Thi Thao TRUONG DAI HOC KHOA HOC TU NHIEN •MOHI^UTtUONO Nghien cuu phuang phap dong hpc trSc quang xac dinh asen nude ngam- De tai cap truang DHKHTN U'ng dung ph^n mSm may tinh giang day va nghien ciai Hoa phan tich: D6'taic4p DHQG _ dau ket (neu co) chua nghiem thu) 2002'• 2003 I ! 2006 2007 | Truang DHKHTN Da nghiem thu ' _ -^ -J Truang DHKHTN Dd nghiem thu ' 19.2 De thi du an, hoac nhiem vu KHCN khac da va dang tham gia vai tir each vi?n Thdi Ca quan qudn ly de Tinh trang de tdi Ten/ Cdp (da nghiem thu/ tdi thudc Chucmg gian chua nghiem thu) trinh (bat dau (neu co) -kk i thiic) Da nghiem thu 2004- : Truang DHKHTN Nghien ciiru phuong phap dong 2005 hoc xiic tac kel hop voi ky thuat chiet pha r^n sir dung nhua tir tinh ^ de xac dinh dang t6n tai lucjng vet i va sieu vet cac kim loai nang- De I tai CO ban- Bo KHCN Trudng DHKHTN Chua nghiC-m thu 2006Nghien ciru cac phucmg phap 2008 thdng ke da bi£n xac dinh nguon g6c va su phan b6 kim loai tren CO so phan tich cac dang kim loai dit va trSm tich De tai co ban- BQ KHCN 20 Giai thuong v£ KHCN va ngoai nuoc • Hinh thuc N'a noi dung giai thucVng TT L 21.1 So lucrng lien sT da dao tao: .21.2 S6 luong NCS dang hucVng dEn; OL 21.3 S6 lucmg thac sT da dao tao: 21.4 Ihong tin chi tiet: ^ ^ ^^^^^ ^ , ^ ^^^ ^^^^^ Ten luan dn cua NCS ( dd bao ve luan dn TS hoac dong lam NCS) U'ng dung ky thuat chiet pha ran va phuong phap phan tich hoa ly hien dai xac djnh lucmg vet kim loai nang mot so doi tuong \ Vai trd huang i ddn (chinh hay phu) Ten NCS, Thai gian ddo tao i i 1 j HD Pham Hong Quan P^^ 2007-2009 Ten luan vdn cua cdc thgc sT ( chi liqt ke nhirng truang hcrp dd hu/mg ddn bao v/^ cdng) Nghien ctju xac djnh thiiy ngan WD bang phucmg phap dpng hpc chinh xiic tac dua tren phan img chi , thi giija sunflt va cac thuoc thir nh(')ni triphcnyl mctan Nghien cuu phutrng phap hoi qui da bien xac dinh mpt so kim loai hon hgp Truang THPH chu Hai Duong ; Ten thgc si Thai gian ddo tgo Ca quan cdng la^ hgc vien, dia chii he ( neu co) Nguyen Thi Hai Yen ^2002-2004 Trung tam phan t Vien vat lieu xa dung- Bp xay dir ID chinh Nguyen Van Dong 2005- 2006 Nguyen Thu Huong Trung tam phanti 2004-2006 : moi truong-Vien i sinh va tai lieu ngh i vu- Bp cong Ar Xac djnh ham luong thiiy ngan HD mau moi trucmg bang chinh phuang phap quang hap thu \ nguyen tir hoa hoi lanh (CVAAS) I Phuang phap trac quang xac dinh dong thai sat va titan xi mang va cot lieu xi mang HD chinh Doan Thj Hung Nghien ciru danh gia mire dp nhiem kim loai nang thuoc dong y HD chinh Nguyen Thi Van Anh Nghien ciru cac dieu kien xac dinh cac dang asen bang phuiTng phap hap thu nguyen tu Ca quan cdng tdc TS NCS, dia chi I he ( neu c6) 2005-2007 2005-2007 HD chinh j I I I Nguyen Thj Thu Hang 2005-2007 Truang THPTba cong Hai Duan( Vien Y hpc cotni) BoYte Tian lich a^nh gfa Mm lupng I HD Sc dang lien ket kim loai chinh i lat va tram tich tai cac b3i thu j gom va tai che chat thai dien, | dien tir Tran Thj Ha 2006-2008 I Jghien ciru phuang phap dpng Ipc trac quang ket hgp vai hoi )ui da bien xac dinh dong thoi cac chat ' HD ; chinh i I Kieu Thu Ha 2006-2008 Tham gia cac to chirc hiep hoi nganh nghe, vien Ban bien lap cac tap chi khoa pc va ngoai nuac, vien cac hoi dong quoc gia, quoc te; Ha Ndi 02 thang ()2 nam 20(19 NGUOI K H \ I (Hp ten va chQ ky) XAC NHAN CUA THU TRUONG DON VI o Ti lHtUpUufV(r \OC ^ ^ \ prtC Pi^CNG TS Ta Ihi Ihao n?'Jc;rjG ti- ^^^ I i/f) HO KHCA H O C / l ^U NHIEN '-'• "^^'^ y DAI HOC QLOC GIA HA NOI LY LICH KHOA H p C THONG TIN CA NHAN Ho va ten: Nguyen Ngoc So'n Ngay sinh: 06-02-1982 Nam/nfr: Nam Ncri sinh: Ha Noi Nguyen quan: Ha Nyi Dia chi thu'ong 1tni hien nay: Nha so Ngo 310 Nguyen Van Cir Phuang: B6D^ Quan: Long Bien Thanh pho: Ha npi D\^n thoai: 04 8.720.415 D|a chi e-mail: S(>nmi(^vnu.edu.vn Mobile: 0915.676.450 7.I lye vi Thac sT: /\ Nam bao ve: 2006 Noi bao ve; ruang Dai hpc Khoa hpc Tu nhien - Dai hpc Quoc gia Ha npi Nganh: Hoa hpc Chuyen nganh: Hoa Phan tich Chiic danh kho;1 hoc : 8.1 Pho giao svr 8.2 Giao Su Chiic danh nj^hi en ciiu: Can bp giang day 10 C hue vu: • 1 CiY quan conj; 1tac Ten ca quan: Truang Dai hpc Khoa hpc Tu nliien, Dai hpc Quoc gia Ha npi Bp mon: Hoa Phan tich Khoa Hoa hpc i)ia chi CO quan: 19 ke Thanh Tong - Ha npi Dien thoai: 04 8.261.856 T ' R I N H D O HOC VAN 12 Qua trinh dao tao Bac dao tao | Noi dao tao Chuyen mon Dai hpc fruorng Dai hpc KHTISf Ha np[ Th^csT I Truong Dai hoc KHTN Ha npi NSm tot nghiep i Hoa Phan tich 2004 ^2006 ^m^nii;^ 13 Cac Idioa dao tao khac Vm bang Ten Khoa dao tao Noi dao tao Thoi gian dao tao t 14 Trinh ngoai nguTT , Ngoai ngir Trinh dg A I Trinh B ; Trinh C Chung ehi QT i Tieng Anh ICINH NGHIEM LAM VIEC VA THANH TICH KHCN 15 Qua trinh cong tac Thoi gian Vj tri cong tac 2006 - Ca quan cong tac Oia chi co cjuan Canbp giang day - Khoa Hoa hpc Truang DH KHTN' 19 Le Ihanh long HN 16 Sach chuyen khao, giao trinh cac bai bao KH da cong bo 16.1 Sach giao trinh TT Noi XB Ten siich Nam XB 16.3 Cac bai bao KH 16.3.1 So bai bao dang cac tap chi nuac: 03 16.3.2 S6 bai bao tham gia cac hoi nghi KH nuac: 16.3.3 Liet ke day du cac bai bao Ten bai bao KH TT , Determination of rare earth impurities in high purity Coupled (ICP-MS) yttrium Plasma by Mass Inductively Spectrometry Tac gia hay Ten ky yeu, dong TG tap chi CB Dong tac gia Tap chi phan tich 2006 hoa, Ly va Sinh hoc j Nam ' Determination Of Copper And Lead In Dong tac gia Pure Lanthanum And Its Compounds By Electrothermal Atomic Tap chi phan tich hoa Ly va Sinh hoc Absorption Spectrometric Method (ET-AAS); I _ _ Determination Of Manganese In Pure Lanthanum And Electrothermal Dong tac gia hoa, Ly va Sinh hoc Its Compounds By Atomic Tap chi phan tich Absorption i I Spectrometric Method (ET-AAS) 17 So phat minh sang che 18 SanphSm KHCN 19 Cac de tai, du an, nhiem vu KHCN cac cap da chu tri hoac tham gia 19.1 De tai, du* an hoac nhiem vu NCKH da va dang chu tri Ten/cap Xay dung quy trinh phan U'ch Thai gian Co quan quan ly DT Tinh trang (bat dau-ket thuc) Thuoc chuang trinh (da nuhien 2007 - 2008 DH KHTN Thiiy ngan (I Ig) bang ky Da nghiem chat lupng I thuat Hydrua hoa vai loai chiit khirNaBn4 vaSnCbNghitMi cim kha nang chiet 2008 - 2009 DH KHTN va lam giau mot so kim loai nSng nuac bang than hoat tinh bien tinh XTV 19.2 De (ai, dir an, hoac nhiem MJ KHCN khac da va dang tham gia viri tu each thii vien Tea/cap 20 Giai thucrng KHCN 2L l);^i) (ao s n i l Thai gian • Co quan quan ly DT Tinh trang d (bat dau-k8t thiic) Thuoc chuong trinh (da nghi?mi .1 D T T S : So lu'9Tig ThS da dao tao Thong tin chi tiet Ten luan van ciia Hiac sT Vai tro en hac sT, Huang dan thai gian dao tao ; Co quan cong tac ciia Thac sT HUNG THONG TIN KHAC VE CAC HOAT DONG KHCN Tham gia cac to chiic hiep hoi nganh nghe, vien Ban bien tap cac tap chi khoa hpc :)ng va ngoai nuac; vien cac hoi dong quoc gia quoc lo: Hd Ndi C t fhdngM XAC NHAN CIJA TBIJ TRliONC DON Vl NGUOI KHAI (Hp ten va chir ky) (Ho len va chCr ky) p^A nam ^'I'j; PHCSS c ^p /\/^'-)i)e^^ hJ^^H '^'^j DA/ HO A '^HHCA HOC ' f "7 ' - ^/^;/t -Ji'^''^ PHIEU DANG KV HUONG DAN SAU DAI HOC TRONG CAC DE TAI KHOA HQC CONG NGHE / Ten cua de idi ludn van/ ludn dn fdu kien) Nghien ciru ddnh gid ngudn gdc vd phdn hd hdm luang kim logi nang mat s6 cdy thudc Tap the hi"/-n;> ddn: T^ Tc: Thi '^'bao Ten NCS' hgc tcn cou hoc (nji, JC co) Ng.nen Van Thudn Ndi Jung chinh cua ludn dn -t-Toi uu hoa cac dieu kien phin lien dong thai lucmg vet \a sieu \et cac kim loai nang bang thiet hi phan tich nguon cao :L'^ ram img plasma- khoi (ICP-MS) Day la thiet bj phan tich hien dai nhat hien mai co a Viet Nam mot vai nam gan da\ va CO uu diem noi bat \ ice phan tich dong thai lucmg \ et va sieu vet cac kim loai cac loai doi tuang khac +Nghicn ciru cac qui trinh xu ly nuu thuoc dong dupe va mau dat + Phan tich danh gia ham luang kim loai nang mot so loai thuoc dong dupe va mpt so don llnuu: dccig di'"c ilaug lu*u hanh tren thj truang + Danh gid su pnal lan kiivi loui ni lii lii ihuoc dong duoc vao thuoc * Danh gia nguO»n goc \a phat tan k.im loai nlng dong duoc theo dieu kien tho nhurrng va tronji ch3n ^ contents in some kmds of medicinal herbs and releasing in to water of cooked medicinal herbs Bachelor thesis, Hanoi University of Science, VNU, 2009 - Nguyen Thi Hong Analysis of some heavy metal contents in herbal medicines using AAS Bachelor thesis, Hanoi University of Science, VNU, 2011 01 Master thesis - Nguyen Van Thuan Evaluation of distribution and sources of heavy metals in some medicinal herbs Master thesis Hanoi University of Science, VNU, 2009 - Publications: 03 publications Ta Thi Thao, Nguyen Thi Van Anh, Nguyen Ngoc Son Assessment of heavy metal contents in herbs using ICP-MS Journal of Science and Technology Vol 48, No 2A, (2010), pp 469-477 Ta Thi Thao, Nguyen Van Thuan, Nguyen Thi Huong fhom Evaluation of distribution and sources of heavy metals in some medicinal herbs and soils using ICPMS technique and multivariate analysis Journal of Chemistry T 48, 4C, (2010) pp 396-401 Ta Thi Thao, Nguyen Van Thuan Evaluation of heavy metal contents in medicinal herbs and soils using ICP- MS technique Journal of Analytical Sciences Vol 15,No.4(2010), pp 223-229 11 Evaluation grade (if the project has been evaluated by the the evaluation committee: excellent, good, fair) PHIEU DANG KY KET QUA NGHIEN CU U KH-CN Ten de tai (hoac du an): B^nh gia s , nhilm kim ,o,i nang thu6c dong dupe v nghien c ^ danh gi nguon goc va su phan bo kim loai nang mot s6 cay thu6c Ma so: QG.09.13 Co- quan chu tri dl tai (hoac du- ^n];T^;^^;;^^^^^;^7l^^ Dja chi: 334- Nguyin Trai, Thanh Xuan, Ha Noi Tel: 84-4- 858.4287 Co- quan quan ly 6e tai (hoac du an): Dai hoc Qu6c Gia Ha Npi Dja chi: 144- Xuan Thuy, Cku Gi^y, Ha Noi Tel: Tong kinh phi thuc chi: 100.000.000 ddng Trong do: - Tii ngan sach Nha nuoc: 100.000.000 d6ng - Kinh phi ciia truong: - Vay tin dung: - Von tu co: - Thu hoi: Th6i gian nghien ciiu: nam Thoi gian bit dau: 04/2009 Thdi gian ket thiic: 04/2011 Ten cac can bo phoi hop nghien cuu: rhs Nguyen Ngoc Soil- Khoa Hoa hoc - Trirang DHKHTN- DHQGHN ThS Nguyen Thi Van Anh- Hoc vien nghien cuu y Dugc co truyen Viet Nam CN Nguyen Van Thudn- Hoc vien cao hoc K18- Truong DH Khoa hoc Tu nhien So dang ky dk tai So chiing nhan dSng ky ket qua nghien ciru: Ngay: Bao mat: a Pho biln rong rai: X b Pho bien han ch§: c Bao mat: T6m tat ket qua nghien cuu: Ket qua khoa hoc: * Da nghien cuu va lira chon dugc cac di6u kien phan tich thich hop di xac dinh liromg vet cac kim loai nang theo phuong phap kh6i ph6 diing ngu6n cao tan cam u-ng plasma (ICP-MS) Tren ca so da tiln hanh x^c nhan gia tri su dung cua phuang phap phan tich nhu- lap duong chu4n phan tich 11 nguyen t6 kim loai nang (Cr, Mn, Fe, Co, Ni, Cu, Zn, Cd, Pb, As va Hg), d^nh gia gioi han phat hicMi gioi han dinh lugng va chinh xac (do diing va chum), hieu su4t thu hoi cua phuang phap phan tich vai cac nln mau thudc dong dugc, mau d^t * Da nghien cuu ap dung quy trinh xu ly mau thu6c dong y, gdm qu\ trinh \u ly mau bang 16 nung, quy trinh xu ly mau bang binh Kendal va quy trinh su dung 16 vi song va lua chgn dugc quy trinh xu ly mau phii hgp voi trang bi hien co la phuang phap kho uol ket hgp hoac xu ly 16 vi song dat hieu su^t thu hdi tir 90-95% va lap tdt CV chua phat hien thay su nhilm ddc kim loai nang cac mau nshien ciru nav Nghien ciru cuna da budc dhu danh gia su phat tan kim loai nang tii dugc lieu vao thudc sac Su phat tan thuoc vao tiing kim loai, mgt sd kim loai phat tan tuong ddi nhieu (tren 30°o) nhu Ni, Cu, Zn Kit qua phan tich ham lugng 11 kim loai nang dat trdng cay thudc va mgt sd cay thudc tai hai dia diem la vuon thudc cung cho thay cd su cao bit thuang mgt sd kim loai Viec su dung chuan thdng ke Student de danh gia cung cho thc4y cd sir khac cd nghTa vl ham lugng trung binh mdi kim loai tai hai dia dilm khac nhau, theo thai gian sinh sdng cua cay va kha nang tich luy sinh hoc cua cay theo tuoi * Dua tren kit qua dat dugc, bang phan tich thdng ke da bien da danh gia dugc nguon goc phat ^^nlili^^^^^T^^^^Ti^—-^^ : r-— • , ., , •^ "S ^^y ^"uoc dong dugc tren dat trdng nghien cuu, tu rut dugc nhan xet vl dilu kien thA v, ~ u , ^x ' • ^ ' ^ " ' ^ ' ? " tho nhuong va cham sdc, ban chat tu n ten cua chat da, gay s , khac co nghTa ve ham l u ^ g cua n,6, s6 kim loai njng khac Ngoai ra, muc tich luy kim loai nSng khac voi tung loa, cay va theo tho, gian khac cung gay bi6n d6i khac ve ham luong truna b,nh V, vay, can co nhtrng nghien cuu cu thi hon voi timg loai cay ,hu6c danh g,a muc dp anh hucmg cua kim loai nang tir co thd dua k a luan chinh xac Ket qu§ ting dung: - Qui trinh phan tich ddng thai tdng ham lugng 11 kim loai nang Cr, Mn Fe, Co, Ni, Mn, Cu, Pb, Cd, Zn, Pb, As va Hg bang phuong phap ICP-MS vdi nhay va chinh xac cao da dugc ung dung di phan tich ham lugng cac kim loai nSng dat, cay thudc va thang thudc ddng dugc cung nhu thudc sSc tren thilt bi ICPMS tai khoa Hda hgc- Truong Dai hgc Khoa hgc Tu nhien- Dai hgc Qude Gia lia Ndi - Da nghien cuu va ii-ng dung phuang phap thong ke da biln dl phan tich tap sd lieu ve ham lugng 11 kim loai nang cay thuoc va dat cay thu6c tai hai dia diem khac de ket luan ve phan bo ham lugng kim loai nang va nguon goc phat t^n kim loai nang vao cay thuoc gop phan on dinh va kiem soat chat lugng dugc lieu tu' goc So lieu thu dugc cung minh hga tot dang bai giang ve phuong phap thdng ke da bi^n chuyen de bat buoc day cho sinh vien cao hoc chuyen nganh Hoa phan tich ciia khoa Hoa 3.San pham ciia de tai: - Ba bai bao khoa hgc da dang tren tap chi khoa hgc chuyen nganh : Ta Thi Thao, Nguyen Thi Van Anh, Nguyen Nggc San Nghien ciru danh gia ham lugng kim loai nang ddng dugc bang phuo-ng phap khdi phd plasma cam ung (ICP-MS) Tgp chi khoa hoc va cong nghe Tap 48, sd 2A (2010), trang 469-477 Ta Thi Thao, Nguyen Van I huSn, Nguyin Thi Huong Thom Nghien cuu danh gia ngudn gdc va phan bd kim loai nang mgt sd ca\ thudc va dcit trdng cav thudc bang phuang phap phan tich ICP-MS va thdng ke da bien Tap chi Hoa hoc T 48, 4C, (2010), trang 396-401 Ta Thi Thao, Nguyen Van ThuSn Ung dung phuang phap khdi phd cao tan plasma cam iing (ICP-MS) dl phan tich danh gia ham lugng kim loai nSng mgt sd cay thudc va dat trdng cay thudc Tap chi Phan tich Hda, Ly va Sinh hgc Viet Nam Tap 15, Sd (2010), trang 223-229 - Dao tao: + Hai cu nhan: - Nguyen Thi Thanh Dao Nghien ciiu danh gia ham lugng asen va thuy ngan mgt loai cay thudc va phat tan vao thudc sac Khoa luan tot nghiep- Khoa Hda hgcTruong DH KHTN, 2009 - Nguyen Thi Hong Phan tich ham lugng mot so kim loai nang thudc dong dugc bang phuang phap ph6 h^p thu nguyen tu Khoa luan t6t nghiep- Khoa Hoa hgc- Truong DH KHTN, 2011 + Mgt thac sT Nguyen Van Thuan Nghien cuu danh gia nguon goc va sir phan bo kim loai nang mgt so cay thuoc.Trudng Dai hgc Khoa hoc Tu nhien DHQCi Ha Noi, 2010 Kien nghj ve quy mo va doi tuong ^p dung nghien cuu: k i bAh ^.^^^5-JM*L'^ >^'^' ';y ... lugng kim loai nang a dat ^-Q 3.5.2 Su phan bo ham lugng kim loai nang mot so cay thuoc ^^ 3.6 Buac dau khao sat su phat tan kim loai nang tu dugc lieu vao thuoc sac 3.7 Danh gia su tich luy kim. .. nhiem doc kim loai nang cac mau nghien cuu Nghien ciiu cung da buac dau danh gia su phat tan kim loai nang tu dugc lieu vao thuoc sac Su phat tiin thuoc vao tirng kim loai, mot so kim loai phat... mg Pb/kg (trong cii hanh), 0,87mg Cd/kg va 226mg Zn/kg (trong re rau dilp), 6,3 mg Cu/kg (trong la cay vimg) Dilu dugc giai thich cay trdng dugc trdng ren viing dk bi d nhiem As va cac kim loai