DSpace at VNU: Screening the optimal ratio of symbiosis between isolated yeast and acetic acid bacteria strain from traditional kombucha for high-level production of glucuronic acid

DSpace at VNU: Screening the optimal ratio of symbiosis between isolated yeast and acetic acid bacteria strain from trad...

Accepted Manuscript Screening the optimal ratio of symbiosis between isolated yeast and acetic acid bacteria strain from traditional kombucha for high-level production of glucuronic acid Nguyen Khoi Nguyen, Phuong Bang Nguyen, Huong Thuy Nguyen, Phu Hong Le PII: S0023-6438(15)30032-3 DOI: 10.1016/j.lwt.2015.07.018 Reference: YFSTL 4812 To appear in: LWT - Food Science and Technology Received Date: 11 March 2015 Revised Date: 26 May 2015 Accepted Date: July 2015 Please cite this article as: Nguyen, N.K., Nguyen, P.B., Nguyen, H.T., Le, P.H., Screening the optimal ratio of symbiosis between isolated yeast and acetic acid bacteria strain from traditional kombucha for high-level production of glucuronic acid, LWT - Food Science and Technology (2015), doi: 10.1016/ j.lwt.2015.07.018 This is a PDF file of an unedited manuscript that has been accepted for publication As a service to our customers we are providing this early version of the manuscript The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain ACCEPTED MANUSCRIPT Screening the optimal ratio of symbiosis between isolated yeast and acetic acid bacteria strain from traditional kombucha for high-level production of glucuronic acid Nguyen Khoi Nguyen a,* Email: nguyenkhoibiotech@gmail.com Phuong Bang Nguyena Email: phuongnguyenbang@gmail.com Huong Thuy Nguyen c Email: nthuong13567@hcmut.edu.vn Phu Hong Le a,b SC RI PT Email: lhphu@hcmiu.edu.vn 11 a 12 Vietnam 13 b 14 City, 70000, Vietnam 15 c 16 University, Ho Chi Minh City, 70000, Vietnam 17 *Corresponding author 18 Tel: +84 08 37244270; Fax: +84 08 37244271; Mobile: +84 938 105157 19 Abbreviations: 20 AAB: acetic acid bacteria 21 GlcUA: glucuronic acid 22 KBC: kombucha 23 LAB: lactic acid bacteria 24 SBT: Sweetened black tea M AN U 10 School of Biotechnology, International University, Vietnam National University, Ho Chi Minh City, 70000, Center of Research and Technology Transfer, International University, Vietnam National University, Ho Chi Minh AC C EP TE D Department of Biotechnology, Faculty of Chemical Engineering, University of Technology, Vietnam National 25 26 27 28 ACCEPTED MANUSCRIPT ABSTRACT 30 Glucuronic acid- a human detoxifying drug can be found in traditional kombucha which is sweetened-black tea 31 fermented by symbiotic microflora between yeast and acetic acid bacteria embedded within a microbial cellulose 32 membrane The main purpose of the study is to obtain the new designed symbiosis from the isolated yeasts and 33 bacterial strains which can produce the high-level glucuronic acid kombucha and avoid unexpected microbial 34 contaminants The isolation, selection and identification showed the best initial combination ratio between Dekkera 35 bruxellensis KN89 and Gluconacetobacter intermedius KN89 is 4Y (yeast):6A (acetic acid -bacteria) in number of 36 living cell per milliliter which produced 175.8 mg L-1 glucuronic acid in 7-day fermentation (P