Establishment of Amblyseius swirskii in greenhouse crops using food supplements tài liệu, giáo án, bài giảng , luận văn,...
Establishment of Amblyseius swirskii in greenhouse crops using food supplements Author(s): Dominiek Vangansbeke , Duc Tung Nguyen , Joachim Audenaert , Bruno Gobin , Luc Tirry & Patrick De Clercq Source: Systematic and Applied Acarology, 21(9):1174-1184 Published By: Systematic and Applied Acarology Society URL: http://www.bioone.org/doi/full/10.11158/saa.21.9.2 BioOne (www.bioone.org) is a nonprofit, online aggregation of core research in the biological, ecological, and environmental sciences BioOne provides a sustainable online platform for over 170 journals and books published by nonprofit societies, associations, museums, institutions, and presses Your use of this PDF, the BioOne Web site, and all posted and associated content indicates your acceptance of BioOne’s Terms of Use, available at www.bioone.org/page/terms_of_use Usage of BioOne content is strictly limited to personal, educational, and non-commercial use Commercial inquiries or rights and permissions requests should be directed to the individual publisher as copyright holder BioOne sees sustainable scholarly publishing as an inherently collaborative enterprise connecting authors, nonprofit publishers, academic institutions, research libraries, and research funders in the common goal of maximizing access to critical research Systematic & Applied Acarology 21(9): 1174–1184 (2016) http://doi.org/10.11158/saa.21.9.2 ISSN 1362-1971 (print) ISSN 2056-6069 (online) Article Establishment of Amblyseius swirskii in greenhouse crops using food supplements DOMINIEK VANGANSBEKE1, DUC TUNG NGUYEN1, 2, JOACHIM AUDENAERT3, BRUNO GOBIN3, LUC TIRRY1 & PATRICK DE CLERCQ1 Laboratory of Agrozoology, Department of Crop Protection, Ghent University, Coupure Links 653, B-9000, Ghent, Belgium Entomology Department, Vietnam National University of Agriculture, Hanoi, Vietnam PCS-Ornamental Plant Research, Schaessestraat 18, B-9070, Destelbergen, Belgium Corresponding author: Dominiek Vangansbeke, E-mail: Dominiek.Vangansbeke@biobestgroup.com Abstract Food supplementation is an emerging strategy to improve the establishment and resilience of generalist predators in greenhouse crops This study was conducted to assess the pre-establishment of the generalist predatory mite Amblyseius swirskii Athias-Henriot (Phytoseiidae) on two ornamental greenhouse crops, chrysanthemum and ivy In the first greenhouse trial, we compared two commercial products consisting of Typha angustifolia pollen and decapsulated brine shrimp cysts (Artemia spp.), respectively, with a powdered artificial diet (AD) as food supplements for the predator on chrysanthemum plants at a constant temperature regime of 25°C at a 16:8 h (L:D) photoperiod A population of A swirskii established well when T angustifolia pollen or the artificial diet were applied, but did not when the Artemia product was used as a supplement A second trial on ivy plants was conducted at two temperature regimes (a constant 25°C and an alternating temperature regime of 30°C/15°C, both at a 16:8 h (L:D) photoperiod), comparing T angustifolia pollen, the artificial diet and a non-commercial strain of Artemia franciscana cysts as food supplements At the constant temperature regime, all three food sources allowed the establishment of A swirskii At the alternating temperature regime, however, T angustifolia pollen and the AD but not A franciscana cysts allowed a population of the predator to establish Failure of establishment on the latter food supplement was attributed to dehydration effects under low humidity conditions in the greenhouse associated with the higher daytime temperatures at the alternating temperature regime In conclusion, this study demonstrated the potential of T angustifolia pollen and an artificial diet as a food supplement to support a population of A swirskii in different ornamental crops Key words: Phytoseiidae, Typha angustifolia, Artemia, artificial diet Introduction In augmentative biological control programs, large numbers of mass-produced beneficial arthropods are released in crops to obtain immediate pest control (Stinner 1977; Collier and Van Steenwyk 2004) Although this type of biological control is now a widely adopted standard practice for pest control, efficacy can be low due to poor establishment of the natural enemies in the crop Moreover, the necessity of periodic releases is not always economically viable for the producers To increase the resilience of beneficial arthropods in crops after release, several strategies can be adopted, such as providing alternative food or hosts, oviposition sites and/or shelters (see Messelink et al (2014) and references therein) Food supplementation to natural enemies has been shown to enhance conservation of predators in the crop and to increase the efficiency of pest control (van Rijn et al 2002; Wäckers et al 2005; Lundgren 2009; Put et al 2012; Messelink et al 2014) In the case of phytoseiid predatory mites, which have become a crucial tool in many integrated pest management strategies (Calvo et al 2015), several studies have demonstrated the potential of different types of 1174 © Systematic & Applied Acarology Society food supplements for their establishment in the crop (e.g van Rijn et al 1999, 2002; Hoogerbrugge et al 2008; Messelink et al 2009; Tuovinen & Lindqvist 2010; Nomikou et al 2010; Kutuk & Yigit 2011; Adar et al 2014; Delisle et al 2014; Leman & Messelink 2014; Duarte et al 2015; Kumar et al 2015) These studies indicate that overall pollen provisioning results in a good establishment of the predatory mite population and subsequent control of the pest, even when the target pest is a notorious pollen-feeder, such as the western flower thrips Frankliniella occidentalis (Thysanoptera: Thripidae) (Hulshof et al 2003) Here, we report the results of two greenhouse experiments in which food supplements for the pre-establishment of A swirskii were tested This generalist predatory mite is an efficient biocontrol agent of key greenhouse pests, including thrips (Messelink et al 2006, 2008; Calvo et al 2011), whiteflies (Nomikou et al 2002; Messelink et al 2008; Calvo et al 2011, 2012) and broad mites (van Maanen et al 2010) Ever since its first commercial introduction in 2005, A swirskii became one of the most successful biocontrol agents in protected cultivation, and has been used in over 50 countries (Calvo et al 2015) We used foods that were previously tested for A swirskii in the laboratory (Nguyen et al 2014a; Vangansbeke et al 2015a) In the first experiment, we compared a commercially available pollen product of T angustifolia (NutrimiteTM), decapsulated cysts of Artemia sp (Artefeed) and an artificial powder diet that was developed by Nguyen et al (2014b) for the pre-establishment of A swirskii on potted chrysanthemum plants In a second experiment on ivy plants we tested the establishment of A swirskii using the same pollen product and artificial diet, but the commercial Artemia product Artefeed, was replaced with dry decapsulated cysts of A franciscana from a non-commercial strain The greenhouse experiment with ivy plants was performed at both a constant and an alternating temperature regime to assess the impact of greenhouse climate conditions on the predator’s establishment Such varying temperature regimes are increasingly being used in an energy-saving strategy by greenhouse growers (Tantau et al 1998; Pollet et al 2009) Evidence has been presented that diurnal temperature ranges (DTR) could compromise the outcome of a biological control program (Vangansbeke et al 2013; Messelink et al 2014; Vangansbeke 2015b, c) Material and Methods Mite rearing Female A swirskii were derived from a laboratory colony maintained at Ghent University (Faculty of Bioscience Engineering, Ghent, Belgium) as described by Nguyen et al (2013) Mites were reared on plastic arenas (10 ×10 × 0.3 cm) on a foam pad in a plastic tray containing water The edges of the arenas were covered with tissue paper to provide free water and prevent the mites from escaping Every other day, broad-leaved cattail pollen (Typha latifolia) was supplied on the arenas as a food source The colony was maintained at 25 ± 1°C, 70 ± 5% RH and a 16:8 h (L:D) photoperiod Greenhouse experiment 1: Chrysanthemum The first experiment was performed in a greenhouse at the Ornamental Plant Research Station (Destelbergen, Belgium) between May and August 2014 on young potted chrysanthemum plants (Dendranthema X grandiflorum) Four young plants with 10 to 12 leaves (ca 12 cm high) were planted in one pot (H 9.4cm, ø 12 cm) Four such pots were placed inside a mesh cage (40 × 90 × 80 cm) and per diet treatment we used mesh cages One mesh cage containing pots with four plants each was considered as one replicate Pots were placed on water-saturated irrigation matting to prevent the predatory mites from migrating 2016 VANGANSBEKE ET AL.:ESTABLISHMENT OF A SWIRSKII USING FOOD SUPPLEMENTS 1175 Five adult females of A swirskii (5 to 10 days old) were transferred to the chrysanthemum plants using a fine brush in each mesh cage In this experiment, we tested food supplements: pollen of narrow-leaved cattail T angustifolia (NutrimiteTM, Biobest N.V., Westerlo, Belgium), dry decapsulated cysts of Artemia sp (Artefeed, Koppert B.V., Berkel en Rodenrijs, The Netherlands) and a powdered artificial diet (AD) developed by Nguyen et al (2014b) A control treatment with A swirskii but without food supplements was included in the experiment The artificial diet was composed of 16.6% sucrose, 16.6% tryptone, 16.6% yeast extract, 6.7% glucose (MP Biomedicals LLC, Illkirch, France), 6.7% fructose (Sigma Aldrich Chemie GmbH, Steinheim, Germany), 16.6% egg yolk powder (Bouwhuis Enthoven BV, Raalte, The Netherlands), 0.13% vitamin mix based on the composition of bovine liver (Vandekerkhove et al 2006) (weight percentages: 25.4 % nicotinic acid, 4.9 % riboflavin, 0.5 % thiamine, 1.5 % vitamin B6, 12.4 % Ca-pantothenate, % folic acid, 0.1 % biotin and 54.2 % vitamin C) and 20% (w/w) dry decapsulated cysts of the brine shrimp A franciscana (Artemia Reference Center (ARC), Ghent, Belgium); all ingredients of the artificial diet were ground to powder using a pestle All diets were applied on the plants using a dusting applicator (Nutrigun, Biobest N.V.) The amount of food applied to the plants was recalculated from 500g/ha, which is the recommended dosage for NutrimiteTM (Biobest, 2015) Every other week, the population growth of the predatory mites was monitored by counting the number of motile stages of A swirskii on 20 leaves per cage in situ After counting, the diets were distributed over the plants Temperature was monitored every minutes and during the experiment an average temperature of 23.9°C and average relative humidity of 61.8% was recorded Although a constant temperature regime of 25°C was set in the greenhouse, actual temperatures fluctuated between 26°C (daytime peak temperature) and 18°C (lowest nighttime temperature) Relative humidity also fluctuated, dropping to about 50% RH along with peak daytime temperatures During the night, lower temperatures were accompanied with high relative humidities of up to 80% Greenhouse experiment 2: Ivy A similar experiment was carried out between January and March 2015 on young ivy plants (Hedera helix L cv “Green Ripple”) Ten cuttings of ivy (5-10 leaves) were planted in one pot (H 9.4cm, ø 12 cm) Nine pots were placed in one mesh cage (similar set-up as that described for experiment 1) and per diet treatment cages were used Counting of the predators and application of the diets were done in the same way as described for experiment The pollen and artificial diets were similar to those described in experiment Dry decapsulated cysts of Artemia franciscana from a non-commercial strain originating from the Great Salt Lake (Utah, USA) were provided by the Artemia Reference Center (ARC, Ghent, Belgium) This experiment was conducted at two different temperature regimes in separate greenhouse compartments In the first greenhouse, a constant temperature of 25°C was set, whereas in the other greenhouse compartment an alternating temperature regime with a daytime temperature of 30°C and a nighttime temperature of 15°C at a 16:8 h (L:D) photoperiod (DTR+15) was chosen However, the actual temperature and relative humidity data recorded in both compartments deviated from the settings For the constant temperature regime, average daytime and nighttime temperatures were 24.0 and 22.4°C, respectively Relative humidity averaged 57% under the constant temperature regime In the treatment where temperatures were allowed to fluctuate, an average daytime temperature of 27.0°C was obtained with an average nighttime temperature of 18.3°C In this regime, relative humidity during the day was on average 54.9%, whereas this was 70.7% during the night Statistical analysis We used repeated measures ANOVA to analyze the population growth of A swirskii as affected by different food supplements (IBM, SPSS Statistics 20) For the first experiment, diet was 1176 SYSTEMATIC & APPLIED ACAROLOGY VOL 21 considered as the between-subject factor and time of sampling as the within-subject factor Population densities at the end of the experiment (16 weeks after the initial introduction) were not normally distributed according to a Kolmogorov-Smirnov test and therefore compared by means of a Kruskal-Wallis analysis Mann-Whitney U tests were used to separate the means In the second experiment on ivy plants, data was analyzed in the same way as in experiment and temperature was added as a between-subject factor The level of significance was set at 0.05 in all tests Results Greenhouse experiment 1: Chrysanthemum The density of A swirskii on the chrysanthemum plants was significantly affected by diet, time of sampling and the interaction thereof (Table 1) Figure shows that supplementation with T angustifolia pollen or the powdered artificial diet resulted in significantly higher predator densities than the treatment with Artemia sp cysts and the treatment without food supplements Two weeks after the introduction of A swirskii (from May to May 21), no predatory mites were observed on the chrysanthemum leaves in the absence of food supplements In general, pollen and the artificial diet resulted in similar mite numbers up to week (Aug 13) Thereafter, higher numbers of A swirskii were counted with the artificial diet as a food supplement until week 12 of the experiment The final monitoring indicated higher phytoseiid numbers in the pollen treatment than the treatment with the artificial diet (Kruskal-Wallis: χ² = 25.544; df =3; p