NGHIÊN cứu TỔNG hợp BIOETHANOL từ DUNG DỊCH THỦY PHÂN vỏ QUẢ CHÙM NGÂY

B GIO DC V O TO TRNG I HC CN TH KHOA CễNG NGH ưưưưưư LUN VN TT NGHIP NGHIấN CU TNG HP BIOETHANOL T DUNG DCH THY PHN V QU CHM NGY CN B HNG DN SINH VIấN THC HIN: TS Hunh Liờn Hng Nguyn Bỏ Thnh; MSSV: 2102393 Ngnh: Cụng ngh Húa hc Khúa 36 Thỏng 12/2014 TRNG I HC CN TH CNG HềA X HI CH NGHAVIT NAM KHOA CễNG NGH c lp T Hnh phỳc Cn Th, ngy thỏng nm 2014 PHIU NGH TI TT NGHIP CHO SINH VIấN NM HC: 2014ư2015 H v tờn cỏn b hng dn: TS Hunh Liờn Hng Tờn ti: NGHIấN CU TNG HP BIOETHANOL T DUNG DCH THY PHN V QU CHM NGY a im thc hin: Phũng thớ nghim Cụng Ngh Hoỏ Hc, Khoa Cụng ngh, Trng i Hc Cn Th Sinh viờn thc hin: Nguyn Bỏ Thnh MSSV: 2102393 Mc ớch ca ti: kho sỏt kh nng s dng v qu Chựm ngõy lm nguyờn liu cho tng hp ethanol sinh hc Ni dung chớnh Phn I: Thy phõn v qu Chựm ngõy + Kho sỏt nh hng ca nng acid n quỏ trỡnh thy phõn + Kho sỏt nh hng ca thi gian n quỏ trỡnh thy phõn + Kho sỏt nh hng ca t l VQCN/dung dch acid n quỏ trỡnh thy phõn + Kho sỏt nh hng ca nhit n quỏ trỡnh thy phõn Phn II: Lờn men sn phm thy phõn v qu Chựm ngõy Cỏc yờu cu h tr: kinh phớ, hoỏ cht v dng c thớ nghim Kinh phớ d trự thc hin ti (d trự chi tit ớnh kốm): 2.000.000 ng DUYT CA B MễN CN B RA TI DUYT CA HI NG THI & XẫT TT NGHIP TRNG I HC CN TH CNG HềA X HI CH NGHAVIT NAM KHOA CễNG NGH c lp T Hnh phỳc Cn Th, ngy thỏng nm 2014 NHN XẫT V NH GI CA CN B HNG DN H v tờn cỏn b hng dn: TS Hunh Liờn Hng Tờn ti: NGHIấN CU TNG HP BIOETHANOL T DUNG DCH THY PHN V QU CHM NGY H v tờn sinh viờn thc hin: Nguyn Bỏ Thnh MSSV: 2102393 Lp: Cụng ngh Hoỏ hc Khoỏ: 36 Ni dung nhn xột: a Nhn xột v hỡnh thc: b Nhn xột v ni dung ca lun ( ngh ghi chi tit v y ) * Cỏc ni dung v cụng vic ó t c (so vi cng lun vn): * Nhng cũn hn ch: c Nhn xột i vi tng sinh viờn thc hin ti: d Kt lun v ngh: im ỏnh giỏ: Cn Th, ngy thỏng nm 2014 Cỏn b hng dn TS Hunh Liờn Hng TRNG I HC CN TH CNG HềA X HI CH NGHAVIT NAM KHOA CễNG NGH c lp T Hnh phỳc Cn Th, ngy thỏng nm 2014 NHN XẫT V NH GI CA CN B PHN BIN H v tờn cỏn b hng dn: TS H Quc Phong Tờn ti: NGHIấN CU TNG HP BIOETHANOL T DUNG DCH THY PHN V QU CHM NGY H v tờn sinh viờn thc hin: Nguyn Bỏ Thnh MSSV: 2102393 Lp: Cụng ngh Hoỏ hc Khoỏ: 36 Ni dung nhn xột: a Nhn xột v hỡnh thc: b Nhn xột v ni dung ca lun ( ngh ghi chi tit v y ) * Cỏc ni dung v cụng vic ó t c (so vi cng lun vn): * Nhng cũn hn ch: c Nhn xột i vi tng sinh viờn thc hin ti: d Kt lun v ngh: im ỏnh giỏ: Cn Th, ngy thỏng nm 2014 Cỏn b phn bin TS H Quc Phong TRNG I HC CN TH CNG HềA X HI CH NGHAVIT NAM KHOA CễNG NGH c lp T Hnh phỳc Cn Th, ngy thỏng nm 2014 NHN XẫT V NH GI CA CN B PHN BIN H v tờn cỏn b hng dn: ThS Nguyn Vn t Tờn ti: NGHIấN CU TNG HP BIOETHANOL T DUNG DCH THY PHN V QU CHM NGY H v tờn sinh viờn thc hin: Nguyn Bỏ Thnh MSSV: 2102393 Lp: Cụng ngh Hoỏ hc Khoỏ: 36 Ni dung nhn xột: a Nhn xột v hỡnh thc: b Nhn xột v ni dung ca lun ( ngh ghi chi tit v y ) * Cỏc ni dung v cụng vic ó t c (so vi cng lun vn): * Nhng cũn hn ch: c Nhn xột i vi tng sinh viờn thc hin ti: d Kt lun v ngh: im ỏnh giỏ: Cn Th, ngy thỏng nm 2014 Cỏn b phn bin Th.S Nguyn Vn t LI CM N Trong quỏ trỡnh thc hin lun tt nghip, tụi ó tri qua khụng ớt nhng khú khn v tr ngi Nhng vi s hng dn v giỳp tn tỡnh ca quý thy cụ, bn bố, gia ỡnh v s n lc ca chớnh bn thõn, tụi ó hon thnh ti lun tt nghip ny Tụi xin gi li cm n sõu sc nht n TS Hunh Liờn Hng, Trng PTN Cụng ngh Húa hc, Khoa Cụng ngh, i Hc Cn Th Cụ ó cho tụi c hi c tham gia nghiờn cu khoa hc v giỳp tụi n vi hng nghiờn cu ny Bờn cnh ú, Cụ cng luụn tn tõm hng dn, ch bo, truyn t kin thc cng nh kinh nghim v to mi iu kin thuõn li tụi hon thnh tt lun ny Xin gi li cm n chõn thnh nht n TS H Quc Phong, Phú Trng B mụn Cụng ngh Húa hc, Khoa Cụng ngh ó tn tỡnh giỳp , hng dn v kin thc chuyờn mụn v thc nghim sut quỏ trỡnh thc hin luõn Tụi xin gi li cm n n tt c cỏc anh, ch hc viờn cao hc v cỏc bn nhúm nghiờn cu khoa hc ti PTN Cụng ngh Húa hc, B mụn Cụng ngh Húa hc ó giỳp v h tr tụi sut quỏ trỡnh thc hin lun ny Cui cựng, tụi xin chõn thnh cm n gia ỡnh, bn bố, nhng ngi luụn quan tõm giỳp v ng viờn tụi sut khong thi gian qua tụi c hon thnh tt lun Cn Th, ngy thỏng nm 2014 S.V Nguyn Bỏ Thnh SVTH: Nguyn Bỏ Thnh i TểM TT Ethanol sinh hc ó tr thnh mt ngun nng lng thay th quan trng Nghiờn cu ny nhm kho sỏt kh nng s dng v qu Chựm ngõy nh nguyờn liu thay th tng hp ethanol sinh hc V qu Chựm ngõy (VQCN) sau x lý s b s c thy phõn bng acid H2SO4 loóng nhm chuyn húa cỏc thnh phn bờn t bo VQCN thnh cỏc loi ng cung cp cho quỏ trỡnh lờn men thnh ethanol Nhng nh hng ca nng acid H2SO4 (2ư8 wt.%), thi gian (2ư8 gi), t l VQCN/dung dch acid (1/10ư1/25 g/mL) v nhit (60ư90 C) n quỏ trỡnh ng húa VQCN ó c nghiờn cu Nng ng ti a thu c t 22.38 g/L, tin hnh thy phõn VQCN vi 6% H2SO4, 90 C, gi v t l VQCN/dung dich acid l 1/10 g/mL Sn phm thy phõn VQCN s c kh c bng Ca(OH)2 trc lờn men nhm lm gim s nh hng ca cỏc hp cht c ch nh cỏc hp cht furan (furfural v hydroxyl methylfurfural), cỏc acid yu v cỏc hp cht phenol n s tng trng ca t bo, tiờu th ng v tớch ly ethanol ca vi sinh vt lờn men Nng ethanol cao nht t c l 7.03 g/L vi nng sut ethanol tng ng 0.379 g ethanol/g ng SVTH: Nguyn Bỏ Thnh ii CHNG KT LUN KIN NGH Phõn tớch thnh phn cỏc loi ng v cỏc hp cht c ch cú sn phm thy phõn, xỏc nh s nh hng ca nhng hp cht c ch ny n s sinh trng v tớch ly ethanol ca vi sinh vt lờn men Nghiờn cu thờm nhng yu t nh hng n quỏ trỡnh lờn men nh: ngun carbon, ngun nit, iu kin nuụi cy (nhit v pH) ti u húa quỏ trỡnh lờn men nhm thu c hm lng ethanol ti u SVTH: Nguyn Bỏ Thnh 41 TI LIU THAM KHO Agarwal, A K., 2007 Biofuels (alcohols and biodiesel) applications as fuels for internal combustion engines Progress in Energy and Combustion Science: 233ư271 Amaglo, N K., R N Bennett, R B Lo Curto, E A Rosa, V Lo Turco, A Giuffrida, A L Curto, F Crea G M Timpo, 2010 Profiling selected phytochemicals and nutrients in different tissues of the multipurpose tree Moringa oleifera L., grown in Ghana Food Chemistry: 1047ư1054 Aro, N., T Pakula M Penttilọ, 2005 Transcriptional regulation of plant cell wall degradation by filamentous fungi FEMS microbiology reviews: 719ư739 Arslan, Y N EkenưSaraỗolu, 2010 Effects of pretreatment methods for hazelnut shell hydrolysate fermentation with Pichia Stipitis to ethanol Bioresource technology: 8664ư8670 Bai, F W., W A Anderson M MooưYoung, 2008 Ethanol fermentation technologies from sugar and starch feedstocks Biotechnology Advances: 89ư105 Balat, M., 2011 Production of bioethanol from lignocellulosic materials via the biochemical pathway: A review Energy conversion and management: 858ư875 Balat, M., H Balat C ệz, 2008 Progress in bioethanol processing Progress in energy and combustion science: 551ư573 Canilha, L., W Carvalho M d G A Felipe, 2008 Xylitol production from wheat straw hemicellulosic hydrolysate: hydrolysate detoxification and carbon source used for inoculum preparation Brazilian Journal of Microbiology: 333ư336 Chandel, A K., F A Antunes, P V de Arruda, T S Milessi, S S da Silva M d G de Almeida Felipe, 2012 Dilute Acid Hydrolysis of AgroưResidues for the Depolymerization of Hemicellulose: StateưofưtheưArt Springer Chandel, A K., S S da Silva O V Singh, 2011 Detoxification of lignocellulosic hydrolysates for improved bioethanol production Biofuel productionưRecent developments and prospects: 225ư246 Chandel, A K., R K Kapoor, A Singh R C Kuhad, 2007 Detoxification of sugarcane bagasse hydrolysate improves ethanol production by Candida shehatae NCIM 3501 Bioresource Technology: 1947ư1950 Chen, W.ưH., Y.ưJ Tu H.ưK Sheen, 2010 Impact of dilute acid pretreatment on the structure of bagasse for bioethanol production International Journal of Energy Research: 265ư274 Cheng, K.ưK., B.ưY Cai, J.ưA Zhang, H.ưZ Ling, Y.ưJ Zhou, J.ưP Ge J.ưM Xu, 2008 Sugarcane bagasse hemicellulose hydrolysate for ethanol production by acid recovery process Biochemical Engineering Journal: 105ư109 CostaưLotufo, L V., M T H Khan, A Ather, D V Wilke, P C Jimenez, C Pessoa, M E A de Moraes M O de Moraes, 2005 Studies of the anticancer potential SVTH: Nguyn Bỏ Thnh 42 of plants used in Bangladeshi folk medicine Journal of Ethnopharmacology: 21ư 30 Cruz, S H., M Batistote J R Ernandes, 2003 Effect of sugar catabolite repression in correlation with the structural complexity of the nitrogen source on yeast growth and fermentation Journal of the Institute of Brewing: 349ư355 Ellis, J T., N N Hengge, R C Sims C D Miller, 2012 Acetone, butanol, and ethanol production from wastewater algae Bioresource technology: 491ư495 Eriksson, T., J Bửrjesson F Tjerneld, 2002 Mechanism of surfactant effect in enzymatic hydrolysis of lignocellulose Enzyme and Microbial Technology: 353ư 364 Fahey, J W., 2005 Moringa oleifera: A Review of the Medical Evidence for Its Nutritional, Therapeutic, and Prophylactic Properties Part Phytochemistry: 123,157 Galbe, M G Zacchi, 2002 A review of the production of ethanol from softwood Applied microbiology and biotechnology: 618ư628 Gỏmez, S., J J GonzỏlezưCabriales, J A Ramớrez, G Garrote M Vỏzquez, 2006 Study of the hydrolysis of sugar cane bagasse using phosphoric acid Journal of food engineering: 78ư88 Gray, K A., L Zhao M Emptage, 2006 Bioethanol Current opinion in chemical biology: 141ư146 Guevara, A P., C Vargas, H Sakurai, Y Fujiwara, K Hashimoto, T Maoka, M Kozuka, Y Ito, H Tokuda H Nishino, 1999 An antitumor promoter from Moringa oleifera Lam Mutation Research/Genetic Toxicology and Environmental Mutagenesis: 181ư188 Ha, M A., D C Apperley, B W Evans, I M Huxham, W G Jardine, R J Viởtor, D Reis, B Vian M C Jarvis, 1998 Fine structure in cellulose microfibrils: NMR evidence from onion and quince The Plant Journal: 183ư190 Hamelinck, C N., G v Hooijdonk A P Faaij, 2005 Ethanol from lignocellulosic biomass: technoưeconomic performance in shortư, middleưand longưterm Biomass and bioenergy: 384ư410 Harmsen, P., W Huijgen, L Bermudez R Bakker, 2010 Literature review of physical and chemical pretreatment processes for lignocellulosic biomass Wageningen UR, Food & Biobased Research Harun, R., M K Danquah G M Forde, 2010 Microalgal biomass as a fermentation feedstock for bioethanol production Journal of Chemical Technology and Biotechnology: 199ư203 Heinze, U W Wagenknecht, 1998 Comprehensive cellulose chemistry: functionalisation of cellulose WileyưVCH, Weinheim Hernỏndez, E., A Garcớa, M Lúpez, J Puls, J C Parajú C Martớn, 2013 Dilute sulphuric acid pretreatment and enzymatic hydrolysis of Moringa oleifera empty pods Industrial Crops and Products: 227ư231 SVTH: Nguyn Bỏ Thnh 43 Howard, R., E Abotsi, E J Van Rensburg S Howard, 2004 Lignocellulose biotechnology: issues of bioconversion and enzyme production African Journal of Biotechnology: 602ư619 Jeong, T S., Y S Kim K K Oh, 2011 Twoưstage acid saccharification of fractionated Gelidium amansii minimizing the sugar decomposition Bioresource technology: 10529ư10534 Kang, H.ưW., Y Kim, S.ưW Kim G.ưW Choi, 2012 Cellulosic ethanol production on temperatureưshift simultaneous saccharification and fermentation using the thermostable yeast Kluyveromyces marxianus CHY1612 Bioprocess and biosystems engineering: 115ư122 Karimi, K., S Kheradmandinia M J Taherzadeh, 2006 Conversion of rice straw to sugars by diluteưacid hydrolysis Biomass and Bioenergy: 247ư253 Kumar, R., G Mago, V Balan C E Wyman, 2009 Physical and chemical characterizations of corn stover and poplar solids resulting from leading pretreatment technologies Bioresource Technology: 3948ư3962 Kurtzman, C P J Pikur, 2006 Taxonomy and phylogenetic diversity among the yeasts Springer Ladisch, M R J A Svarczkopf, 1991 Ethanol production and the cost of fermentable sugars from biomass Bioresource technology: 83ư95 Laluce, C., J O Tognolli, K F De Oliveira, C S Souza M R Morais, 2009 Optimization of temperature, sugar concentration, and inoculum size to maximize ethanol production without significant decrease in yeast cell viability Applied microbiology and biotechnology: 627ư637 Laopaiboon, P., A Thani, V Leelavatcharamas L Laopaiboon, 2010 Acid hydrolysis of sugarcane bagasse for lactic acid production Bioresource Technology: 1036ư 1043 Larue, F., S LafonưLafourcade P RibereauưGayon, 1980 Relationship between the sterol content of yeast cells and their fermentation activity in grape must Applied and environmental microbiology: 808ư811 Lebeau, T., T Jouenne G A Junter, 1997 Simultaneous fermentation of glucose and xylose by pure and mixed cultures of Saccharomyces cerevisiae and Candida shehatae immobilized in a twoưchambered bioreactor Enzyme and Microbial Technology: 265ư272 Lodder, J N J Kregerưvan Rij, 1952 The yeastsưa taxonomic study The yeastsưa taxonomic study.: Martn, C., M Galbe, C F Wahlbom, B HahnưHọgerdal L J Jửnsson, 2002 Ethanol production from enzymatic hydrolysates of sugarcane bagasse using recombinant xyloseưutilising Saccharomyces cerevisiae Enzyme and Microbial Technology: 274ư282 Martớn, C., A Moure, G Martớn, E Carrillo, H Domớnguez J C Parajú, 2010 Fractional characterisation of jatropha, neem, moringa, trisperma, castor and SVTH: Nguyn Bỏ Thnh 44 candlenut seeds as potential feedstocks for biodiesel production in Cuba Biomass and Bioenergy: 533ư538 Martinez, A., M E Rodriguez, S W York, J F Preston L O Ingram, 2000 Effects of Ca (OH) treatments (overliming) on the composition and toxicity of bagasse hemicellulose hydrolysates Biotechnology and Bioengineering: 526ư 536 Millati, R., C Niklasson M J Taherzadeh, 2002 Effect of pH, time and temperature of overliming on detoxification of diluteưacid hydrolyzates for fermentation by Saccharomyces cerevisiae Process Biochemistry: 515ư522 Miller, G L., 1959 Use of dinitrosalicylic acid reagent for determination of reducing sugar Analytical chemistry: 426ư428 Mohibbe Azam, M., A Waris N M Nahar, 2005 Prospects and potential of fatty acid methyl esters of some nonưtraditional seed oils for use as biodiesel in India Biomass and Bioenergy: 293ư302 Mosier, N., C Wyman, B Dale, R Elander, Y Lee, M Holtzapple M Ladisch, 2005 Features of promising technologies for pretreatment of lignocellulosic biomass Bioresource technology: 673ư686 Mtui, G Y., 2009 Recent advances in pretreatment of lignocellulosic wastes and production of value added products African Journal of Biotechnology: Mussatto, S I I C Roberto, 2001 Hydrolysate detoxification with activated charcoal for xylitol production by Candida guilliermondii Biotechnology Letters: 1681ư 1684 Mussatto, S I I C Roberto, 2004 Alternatives for detoxification of dilutedưacid lignocellulosic hydrolyzates for use in fermentative processes: a review Bioresource technology: 1ư10 Ndabigengesere, A K Narasiah, 1996 Influence of operating parameters on turbidity removal by coagulation with Moringa oleifera seeds Environmental technology: 1103ư1112 Nigam, J., 2002 Bioconversion of waterưhyacinth (Eichhornia crassipes) hemicellulose acid hydrolysate to motor fuel ethanol by xylosefermenting yeast Journal of Biotechnology: 107ư116 Nigam, P S A Singh, 2011 Production of liquid biofuels from renewable resources Progress in Energy and Combustion Science: 52ư68 Olsson, L B HahnưHọgerdal, 1996 Fermentation of lignocellulosic hydrolysates for ethanol production Enzyme and Microbial Technology: 312ư331 Palmqvist, E B HahnưHọgerdal, 2000a Fermentation of lignocellulosic hydrolysates I: inhibition and detoxification Bioresource technology: 17ư24 Palmqvist, E B HahnưHọgerdal, 2000b Fermentation of lignocellulosic hydrolysates II: inhibitors and mechanisms of inhibition Bioresource technology: 25ư33 SVTH: Nguyn Bỏ Thnh 45 Palmqvist, E., B HahnưHọgerdal, M Galbe G Zacchi, 1996 The effect of waterư soluble inhibitors from steamưpretreated willow on enzymatic hydrolysis and ethanol fermentation Enzyme and Microbial Technology: 470ư476 Palonen, H., 2004 Role of lignin in the enzymatic hydrolysis of lignocellulose VTT Technical Research Centre of Finland Parawira, W M Tekere, 2011 Biotechnological strategies to overcome inhibitors in lignocellulose hydrolysates for ethanol production: review Critical reviews in biotechnology: 20ư31 Park, I., I Kim, K Kang, H Sohn, I Rhee, I Jin H Jang, 2010 Cellulose ethanol production from waste newsprint by simultaneous saccharification and fermentation using Saccharomyces cerevisiae KNU5377 Process Biochemistry: 487ư492 Persson, P., J Andersson, L Gorton, S Larsson, N.ưO Nilvebrant L J Jửnsson, 2002 Effect of different forms of alkali treatment on specific fermentation inhibitors and on the fermentability of lignocellulose hydrolysates for production of fuel ethanol Journal of agricultural and food chemistry: 5318ư5325 Pessani, N K., H K Atiyeh, M R Wilkins, D D Bellmer I M Banat, 2011 Simultaneous saccharification and fermentation of Kanlow switchgrass by thermotolerant Kluyveromyces marxianus IMB3: The effect of enzyme loading, temperature and higher solid loadings Bioresource Technology: 10618ư10624 Philippidis, G P T K Smith, 1995 Limiting factors in the simultaneous saccharification and fermentation process for conversion of cellulosic biomass to fuel ethanol Applied Biochemistry and Biotechnology: 117ư124 Prakasham, R., R S Rao P J Hobbs, 2009 Current trends in biotechnological production of xylitol and future prospects Current Trends in Biotechnology and Pharmacy: 8ư36 Purwadi, R., C Niklasson M J Taherzadeh, 2004 Kinetic study of detoxification of diluteưacid hydrolyzates by Ca (OH) Journal of biotechnology: 187ư198 Qing, Q., B Yang C E Wyman, 2010 Impact of surfactants on pretreatment of corn stover Bioresource technology: 5941ư5951 Ramachandran, K B M A Hashim, 1990 Simulation studies on simultaneous saccharification and fermentation of cellulose to ethanol The Chemical Engineering Journal: B27ưB34 Ranatunga, T D., J Jervis, R F Helm, J D McMillan R J Wooley, 2000 The effect of overliming on the toxicity of dilute acid pretreated lignocellulosics: the role of inorganics, uronic acids and etherưsoluble organics Enzyme and microbial technology: 240ư247 Rashid, U., F Anwar, B R Moser G Knothe, 2008 Moringa oleifera oil: A possible source of biodiesel Bioresource Technology: 8175ư8179 SVTH: Nguyn Bỏ Thnh 46 Ratanakhanokchai, K., R Waeonukul, P Pason, C Tachaapaikoon, K L Kyu, K Sakka, A Kosugi Y Mori, 2013 Paenibacillus curdlanolyticus Strain Bư6 Multienzyme Complex: A Novel System for Biomass Utilization Richter, N., P Siddhuraju K Becker, 2003 Evaluation of nutritional quality of moringa ( Moringa oleifera Lam.) leaves as an alternative protein source for Nile tilapia (Oreochromis niloticus Lam.) Aquaculture: 599ư611 Rocha, G., C Martin, I B Soares, A M Souto Maior, H M Baudel C A Moraes de Abreu, 2011 Dilute mixedưacid pretreatment of sugarcane bagasse for ethanol production biomass and bioenergy: 663ư670 Sabale, V., V Patel, A Paranjape, C Arya, S Sakarkar P Sabale, 2008 Moringa oleifera (Drumstick): an overview Pharmacognosy reviews: 7ư13 Saha, B C M A Cotta, 2008 Lime pretreatment, enzymatic saccharification and fermentation of rice hulls to ethanol Biomass and Bioenergy: 971ư977 Sarkar, N., S K Ghosh, S Bannerjee K Aikat, 2012 Bioethanol production from agricultural wastes: An overview Renewable Energy: 19ư27 Sherrard, E F Kressman, 1945 Review of processes in the United States prior to World War II Industrial & Engineering Chemistry: 5ư8 Taherzadeh, M J K Karimi, 2007a Acidưbased hydrolysis processes for ethanol from lignocellulosic materials: a review BioResources: 472ư499 Taherzadeh, M J K Karimi, 2007b Enzymaticưbased hydrolysis processes for Ethanol BioResources: 707ư738 Talebnia, F., D Karakashev I Angelidaki, 2010 Production of bioethanol from wheat straw: an overview on pretreatment, hydrolysis and fermentation Bioresource Technology: 4744ư4753 Torija, M J., N Rozes, M Poblet, J M Guillamún A Mas, 2003 Effects of fermentation temperature on the strain population of Saccharomyces cerevisiae International journal of food microbiology: 47ư53 Tsigie, Y A., C.ưY Wang, C.ưT Truong Y.ưH Ju, 2011 Lipid production from Yarrowia lipolytica Po1g grown in sugarcane bagasse hydrolysate Bioresource technology: 9216ư9222 Tsigie, Y A., C.ưH Wu, L H Huynh, S Ismadji Y.ưH Ju, 2013 Bioethanol production from Yarrowia lipolytica Po1g biomass Bioresource Technology: 210ư216 Van Maris, A J., A A Winkler, M Kuyper, W T De Laat, J P Van Dijken J T Pronk, 2007 Development of efficient xylose fermentation in Saccharomyces cerevisiae: xylose isomerase as a key component Springer Walford, S., 2008 Sugarcane bagasse: how easy is it to measure its constituents? South African Sugar Technologists' Association Wang, Z., J Zhuge, H Fang B A Prior, 2001 Glycerol production by microbial fermentation: a review Biotechnology Advances: 201ư223 SVTH: Nguyn Bỏ Thnh 47 Watanabe, I., N Miyata, A Ando, R Shiroma, K Tokuyasu T Nakamura, 2012 Ethanol production by repeatedưbatch simultaneous saccharification and fermentation (SSF) of alkaliưtreated rice straw using immobilized Saccharomyces cerevisiae cells Bioresource technology: 695ư698 Wyman, C., 1996 Handbook on bioethanol: production and utilization CRC press Wyman, C E., 2008 Cellulosic Ethanol: A Unique Sustainable Liquid Transportation Fuel MRS Bulletin: 381ư383 Xiao, C., R Bolton W Pan, 2007 Lignin from rice straw kraft pulping: Effects on soil aggregation and chemical properties Bioresource technology: 1482ư1488 Yang, B C E Wyman, 2008 Pretreatment: the key to unlocking lowcost cellulosic ethanol Biofuels, Bioproducts and Biorefining: 26ư40 Zhang, W., Y Lin, Q Zhang, X Wang, D Wu H Kong, 2013 Optimisation of simultaneous saccharification and fermentation of wheat straw for ethanol production Fuel: 331ư337 SVTH: Nguyn Bỏ Thnh 48 PH LC PH LC NG CHUN CA NNG NG KH Thuc th DNS Cõn 1.6 g NaOH, hũa tan vo 30 mL nc ct c dung dich A Cõn g 3,5ưdinitrosalicylic (DNS), hũa tan vo dung dch A c nung trờn bp iu nhit 70 C Cõn 30 g mui kộp NatriưKali tartrate, hũa tan 50 mL nc ct, un v khuy u trờn bp iu nhit 70 C, c dung dch B Trn hai dung dch A v B li vi nhau, thờm nc ct nh mc 100 mL Thuc th DNS cn c gi iu kin trỏnh ỏnh sỏng, bo qun C v ch s dng thi gian ngn ng chun ca Dưglucose y = 0.6206x + 0.0149 R = 0.9992 Mt quang 1.5 0.5 0 0.5 1.5 2.5 Nng ng, g/L ng chun ca Dưglucoese bc súng 575 nm SVTH: Nguyn Bỏ Thnh 49 PH LC PH LC XC NH NNG ETHANOL thu c lng ethanol sau quỏ trỡnh lờn men, phng phỏp chng ct s c s dng Ly dung dch lờn men cho vo bỡnh cu hai c: mt c c ni vi ng sinh hn, bờn cú ng ng dn hi ru v c ngng t bi h thng lm lnh bng nc bao quanh bờn ngoi; c cũn li c gi kớn bng nỳt cao su bờn c gn nhit k xỏc nh nhit Tip theo, lờn bp iu nhit v tin hnh chng ct cho n hm lng ethanol thu c thay i khụng ỏng k thỡ dng li C s khoa hc ca phng phỏp chng ct ny l quỏ trỡnh dựng phn riờng hn hp lng thnh cỏc cu t riờng bit da vo bay hi khỏc ca cỏc cu t hn hp (ngha l cựng mt nhit ỏp sut hi bóo ho ca ca cỏc cu t l khỏc nhau) ỏp sut thng thỡ nhit sụi ca ethanol l 78.3 C v ca nc l 100 C Do ú, chng ct, ethanol s bay hi trc v nhanh hn nc, vỡ th s tỏch c ethanol nc Lng ethanol thu c sau chng ct s c pha loóng vi nc ct t l xỏc nh v nng ethanol c xỏc nh bng cn k SVTH: Nguyn Bỏ Thnh 50 PH LC PH LC 3ư1 KT QU PHN TCH THNG K NH HNG CA NNG ACID N NNG NG TNG tưTest: TwoưSample Assuming Equal Variances Mean Variance Observations Pooled Variance Hypothesized Mean Difference df t Stat P(T