FOOD ANALYSIS VITAMIN C ANALYSIS Group Members: Hai Luu Le 13116071 Cat Minh Vu Quang 13116072 Van Tuan Ma 13116181 Manh Quyet Pham 13116112 Supervisor: Hoang Du Le INTRODUCTION • METHODS • COMPARISION • CONTENT INTRODUCTION Acid ascorbic (AA) Acid dehydroascorbic (DAA) METHODS TITRATION PHOTOMETRIC HPLC IODATE A PRINCIPLE KIO3 + 5KI + 6HCl 3I2 + 6KCl + 3H2O Acid ascorbic + I2 2I + Acid dehydroascorbic B REQUIRED INSTRUMENTS C CHEMICALS HCl 1% KIO3/KI 0.001N Starch indicator: solution 0.5% or 1% D SAMPLE PREPARATION E TITRATION KIO3/KI 0.001 N Vitamin C extract Starch indicator solution INDOPHENOL A PRINCIPLE 10 B REQUIRED INSTRUMENTS 11 C CHEMICALS HPO3 2% or CH3COOH 2% Ascorbic acid 2,6-Dichlorophenol indophenol (DCIP) 12 D PHOTOMETRIC 13 14 HPLC A PRINCIPLE Sample Extract Applied to the HPLC Determine the acid ascorbic 15 B MAIN EQUIPMENT 16 C CHEMICALS Ascorbic acid Methanol Deionized water times H2SO4 Acetonitrile 17 D SAMPLE PREPARATION 18 E.High Performance Liquid Chromatography 19 COMPARISION Titration Photometric HPLC 20 21 [...]... C CHEMICALS HPO3 2% or CH3COOH 2% Ascorbic acid 2,6-Dichlorophenol indophenol (DCIP) 12 D PHOTOMETRIC 13 14 HPLC A PRINCIPLE Sample Extract Applied to the HPLC Determine the acid ascorbic 15 B MAIN EQUIPMENT 16 C CHEMICALS Ascorbic acid Methanol Deionized water 2 times H2SO4 Acetonitrile 17 D SAMPLE PREPARATION 18 E.High Performance Liquid Chromatography 19 COMPARISION Titration Photometric... EQUIPMENT 16 C CHEMICALS Ascorbic acid Methanol Deionized water 2 times H2SO4 Acetonitrile 17 D SAMPLE PREPARATION 18 E.High Performance Liquid Chromatography 19 COMPARISION Titration Photometric HPLC 20 21