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Nghiên cứu tác dụng kháng vi khuẩn sâu răng streptococcus mutans của dịch chiết lá sim rhodomytus tomentosa (aiton) hassk (LV01296)

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    Streptococcus mutans  Rhodomytus tomentosa (Aiton) Hassk       Streptococcus mutans  Rhodomytus tomentosa (Aiton) Hassk  60 42 01 14      i   c                           s       s                            -     ii                  Streptococus mutans  -2011.44           iii  Trang  i  ii  iii  vii  viii  ix  1  1  3  4 4.  4  5  5 1.1 5  5 . 7 1.2.  Streptococcus mutans 8  11  11 1.3.2.   13 1.4.   15 1.4.1. -ATPase 15  17 . 18 . 21 iv 1.5.  23  24 . 26 (replacement therapy) 27 1.5.4. Vacxin 28 1.6. 28 1.7. Rhodomytus tomentosa) 31  32 2.1.  32 2.1.1. . 32  32  32  33  33  33  34  35 2.2.4.  37 2.2.5.  40  41 S. mutans 41 3.1.1.h acid () 41  42  43  43  46  49 v  S. mutans 53 3.4.1.  ATPase 53 3.4.2.    54 3.5. PS. mutans 55 3.5.1. Sc S. mutans 55 S. mutans  56 3.5.3. S. mutans  57 3.6            Chromobacterium violaceum KCTC 2789 58  61  62  63  68 vi   TSA tryptic soy agar PTS     sugar-phosphotransferase system) ATPase enzyme F  ATPase Gtf enzyme glucosyltransferase DMSO EtOAc MeOH EPS EtOH dymethyl sulfoxide Ethyl acetate Methanol Exopolysachacride Ethanol TLC  NMR  QCC  QSS quorum sensing system CC  vii  Trang .  48 -ATPase 54  55 . 58 viii  Trang .  6 .  7 . Streptococcus mutans 10 .  12 .  ATPase 16 . Rhodomyrtus tomentosa (Aiton) Hask ) 31 2.1.S. mutans 36 .cidS. mutans  42 . S. mutans 43 . 44 .  44 .  45 . S. mutans 46 .c 47 .  47  .               48 [...]... Streptococci) ỉ Streptococcus mutans suis, mutans Streptococcus sobrinus [32] 1924 S mutans ị ổ 13 T 60 ẩ ỉ ị Tổ 7 ỉ ị f [27] N a, N ổ ẩ ẳ ị Streptococcus mutans Streptococcus mutans T "mutans streptococci" Streptococcus mutans ổ Streptococcus [32] S mutans [21], [27], [32] ỉ S S mutans : 10 ; ằ ; Hì 1.3 Ả ể ệ ử ị ẩ Streptococcus mutans 6500 V ổ ẩ S mutans ẩ ẩ [27] N S mutans M ẩ - TP ằ [11] B ẩ Streptococcus. .. ị ẩ ằ S mutans N ị ẩ X : “Ng ê g ră g Streptococcus mutans g ẩ Rhodomyrtus tomentosa (Aiton) Hassk 2 M í Đ ị g ê ẩ Streptococcus mutans “Rhodomyrtus tomentosa ” ằ K ẩ 4 3 N ệ g ê 31 Đ ẩ S mutans ị :i - pH drop), ii f f 32 T ẩ 33 N ẩ S mutans 34 Đ S mutans ị PTS S mutans cid ằ -ATPase 35 Đ f S mutans f 36 Đ QSS ẩ Chromobacterium violaceum KCTC 2897 4 Đó g góp ớ Đ ị ẩ Rhodomyrtus tomentosa) mutans ị... ẩ [27] Vi ẩ ỵ [32] ẩ f ị ổ T “ ổ ” Đ f ổ L ị [32] Q T 8 ẽ 1.2) N ẩ ẩ ẩ ị T ẩ ỉ 1 ổ K ẩ ẩ ẽ S ẩ ẽ [27] ẩ ẩ Streptococcus Streptococcus mutans, Streptococcus sorbinus Lactobacillus T S mutans ị N ổ G ẩ g [32] V ẩ 1.2 V ẩ ẩ Streptococcus mutans Streptococcus ẩn Gram (+), ẩ M 9 ổ f …S N RN 16S Streptococci [32] T Pyrogenic Bovis Streptococcus Streptococcus pneumoniae thermophilus Streptococcus Streptococcus... Streptococcus mutans ị S mutans [27] V ẩ ị PS PS f biofilm S S mutans 2 N ằ S mutans, ẩ f (anti- -biofilm S mutans ằ H : ẩ ẩ ; ẩ ẩ ị ; ằ ẩ T ẩ V ẩ ổ ẩ f ị [31] V ẩ [5], [6], [26] N ị ỞV N ẩ N [5], [6], [35] N α- P ị 50 TP T ịM f PTS - N µM N ị N S Q Hopea odorata Roxb), [4] S Syzygium 3 polyanthum W W cid ị ẩ ATPase, PTS V N - S mutans GS-5 2 S mutans [4] T V [28], [29], [30], [35], [36] ị (Rhodomyrtus tomentosa. .. mutans S V S mutans ẩ S se (AgDS) S O S 4 aguB petruscine carbanoyl transferase, aguD aguA aguC S S ẽ ẩ S mutans f S 1.4.3.4 Lên men malolactic L f ML [37] T o L-malic dicarbo L- O2 ị cid ẩ Lactobacillus, Leuconostoc L- decarb N ổ TP S L B Streptococcus Q ML + M 2+ S + )- TP 21 L-malic o LO2 G S &M Streptococcus mutans U 159 [37] smu 0123 ML L B Kh ML K 40 S mutans ML V ML Lị 1.4.4 Bả ệ ử ữ ạ p S mutans. .. glucans s ẩ ẩ ẩ co S mutans f K S mutans f M ổ EPS PS gtf T f S mutans polysacharide f S h mutans Đ ị t i biofilm N ằ S mutans ẩ acid (anti-acidogeneic agents) f - f S mutans S 15 1.4 Sự í g ẩ g ệ g ẩ ẩ V ẩ ? 1.4.1 Bơ pr F-ATPase 15 F-ATPase ằ ị TP TP ẩ f - ẩ ổ ị (pHi) [1] F- TP 8 ị ổ TP proton [32] Tổ 0 ằ ị ổ ị , , ,  1  F- TP F1 ổ ị 0 TP M 1 ỉ ị 16 Hì 1.5 Sơ ồ ấ K ạ F – ATPase ẩ Streptococcus i... n f Gf ẩ [12] Streptococcus mutans ị : f ; K PS S mutans [24], PS [25] S ẩ glucosyltransferases (Gtf fructosyltransferases (Ftfs) [12], [32] G f (1 → 3) 16 ổ S mutans EPS kh Streptococci ba ổ S .mutans Gf G fB Gtf Gf G fB ổ ổ α-1,3-linked glucose, GtfC sinh ổ glucans can α-1 6- Gf 14 ổ Gf s ổ S mutans ẩ K ỉ ẩ P Streptococci Veillonella spp t Actinomyces and s tr khi Gtfs G ổ ans ị S mutans s glucans... Quivey [35] ị S mutans, S salivarius S sanguis - TP TP N ATPas ị TP ằ G ị S 60 mutans (pH 5,0); S salivarius 70 S sanguis (pH TP Đ 6 0; 7 0; ị ẩ - TP V ẩ Streptococcus 1-F0 ổ TP 8 0 17 [1] - ẩ + ằ T ằ 1-F0 60 ị Rõ TP TP - ẩ [1] 1.4.2 Sự ổ g b ẩ í g ẩ ằ [32] V N ẩ i S Streptococci N [1], [32] ỉ , ằ S ỉ Q [35] S mutans sinh 70 T S mutans 50 ổ Đ 14:0 16:0 ằ ẳ 18:0 ị c ẩ ẩ 18 ổ Đ S17 S mutans ẩ ị T 917... S mutans 51 3 13 S S : S 5 : 2) 51 3 14 P ổ NMR ị S S 52 3.15 Enzyme PTS 3 16 f Sc 54 ẩ S mutans ……………………………………………………………… 3 17 56 f Sc ẩ S mutans …………………………………………………… … 56 3 18 Sc 0,3% 3 19 ổ f S S mutans …… … 57 ổ ẩ Choromobacterium violaceum KCTC 2897 59 1 MỞ ĐẦU 1 L ổ S Tổ Y ổ ỉ [9] T M V N 09 2012 90 V R N ổ [10] Đ N K 84 Đ ổ T V B N ẩ N ẩ cid M ẽ ẩ ẩ K ằ ị ẽ cid [27] Streptococcus. .. ẩ Streptococcus N S 3 O2 NH3 N ẽ 3 N [8], [32] N + 4 S mutans 11 ẩ [12], [24], [25] Đ ẩ [24], [25] C [32] S mutans T S mutans ằ 1.3 Mả g b ị ră g (dental plaque), b 1.3.1 Sự ì ả gb ră g M f ẩ Q 3 f M 1 4 S [32] P R 500 [32] B ẩ ẩ K ( 40 Ở ị M ỉ T ẩ ẽ ra ase ổ Fusarium nucleatumn ị ị [32] 12 A Vi khuẩ chuy c v n B nb m tc C S ị ng g n k t c th vi khuẩ c ị n y u c S ẩ ổ biofilm ng th i v i s sinh tổng .     Streptococcus mutans Rhodomyrtus tomentosa (Aiton) Hassk .   Streptococcus mutans .    Streptococcus mutans  Rhodomytus tomentosa (Aiton) Hassk  60 42 01.    Streptococcus mutans  Rhodomytus tomentosa (Aiton) Hassk 

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