Luận văn thạc sĩ Kỹ thuật hóa học: Nghiên cứu chiết xuất và ứng dụng các polyphenol từ cây hương thảo (Rosmarinus officinalis)

TÓM TҲT LUҰ19Ă1 Mөc tiêu cӫa ÿӅ tài luұQYăQQj\OjQJKLrQFӭu chiӃt xuҩt và ӭng dөng các polyphenol tӯ Fk\KѭѫQJWKҧo Rosmarinus officinalis QKҵPÿiQKJLiKRҥWWtQKNKiQJR[\KyDFӫD FiFKӧSFKҩWQj\.. A

ĈҤI HӐC QUӔC GIA THÀNH PHӔ HӖ CHÍ MINH

Chuyên ngành: KӺ THUҰT HÓA HӐC

Mã sӕ: 60.52.03.01

LUҰ19Ă17+ҤC SƬ

&Ð1*75Î1+ĈѬӦ&+2¬17+¬1+7Ҥ, 75ѬӠ1*ĈҤ,+Ӑ&%È&+.+2$± Ĉ+4*± HCM

LӠI CҦ0Ѫ1

/XұQYăQÿѭӧFKRjQWKjQKWҥL7UѭӡQJĈ+%iFK.KRDĈ+4XӕF*LD7S+&0 Trong TXiWUuQKOjPNKyDOXұQWӕWQJKLӋSHPÿmQKұQÿѭӧFUҩWQKLӅXVӵJL~SÿӥÿӇKRjQWҩWOXұQYăQ

7UѭӟFWLrQHP[LQJӱLOӡLFҧPѫQFKkQWKjQKTKҫ\/r;XkQ7LӃQ ÿmWұQWuQKKѭӟQJGүQWUX\ӅQÿҥWNLӃQWKӭFNLQKQJKLӋPFKRW{LWURQJVXӕWTXiWUuQKWKӵFKLӋQOXұQYăQWӕWQJKLӋSQj\

;LQJӱLOӡLFҧPѫQÿӃQTXêTKҫ\Cô Khoa ӻWKXұW+yDKӑF7UѭӡQJĈ+%iFKKhoa

Tp HCMQKӳQJQJѭӡLÿmWUX\ӅQÿҥWNLӃQWKӭFTXêEiXFKRHPVXӕWWURQJWKӡLJLDQKӑFWұSYӯDTXD

6DXFQJ[LQJӱLOӡLFҧPѫQÿӃQJLDÿuQKEҥQEqÿmOX{QÿӝQJYLrQJL~SÿӥW{LWURQJTXiWUuQKOjPOXұQOXұQYăQ

0ӝWOҫQQӳD[LQFKkQWKjQKFҧPѫQ

TÓM TҲT LUҰ19Ă1

Mөc tiêu cӫa ÿӅ tài luұQYăQQj\OjQJKLrQFӭu chiӃt xuҩt và ӭng dөng các polyphenol

tӯ Fk\KѭѫQJWKҧo (Rosmarinus officinalis) QKҵPÿiQKJLiKRҥWWtQKNKiQJR[\KyDFӫD

FiFKӧSFKҩWQj\ ÈSGөQJ TX\WUuQKÿmQJKLrQFӭXEӣLQKyPFӫD76/r;XkQ7LӃQYjRFKLӃW[XҩWSRO\SKHQROWӯOiKѭѫQJWKҧRTX\ P{ JQJX\rQOLӋX NK{ÿҥW KLӋXVXҩWNKRҧQJJFDRJQON

1JRjLUDSKѭѫQJSKiSOXkQSKLrQWӯQJELӃQFzQÿѭӧFVӱGөQJÿӇNKҧRViW ҧQKKѭӣQJcác \ӃXWӕҧQKKѭӣQJÿӃQTXiWUuQKVҩ\SKXQ Các ÿLӅu kiӋn tӕLѭX ÿӇ tҥo ra bӝt KѭѫQJthҧo bao gӗm QKLӋWÿӝÿҫX YjR NK{QJ NKtVҩ\Oj o&QӗQJÿӝFKLӃW[XҩWOj % ZZ WӹOӋ*$0'OjZZ WӹOӋUҳQ - OӓQJOjJP/ YjWӕFÿӝQҥSOLӋX10 (mL/phút) %ӝWFyKjPOѭӧQJSRO\SKHQRO3 PJ*$(JEӝW  YjKLӋXVXҩWFӫDTXitrình là 63,2 (% w/w)

+ѫQQӳDEӝWKѭѫQJWKҧRÿѭӧFÿiQKJLiKRҥWWtQKNKiQJR[\EҵQJSKѭѫQJSKiSӭFFKӃJӕFWӵGR'33+ YjӭFFKӃSHUR[\KyDOLSLGWӃEjRQmRFKXӝWWKӱQJKLӋP MDA) giá trӏ IC50 cӫa bӝt sҩy phun cao KѫQYLWDPLQ&Oҫn và WKҩSWUROR[7,OҫQ, cho thҩy bӝt sҩy phun có tiӅPQăQJNKiQJR[\Pҥnh nên có thӇ ӭng dөQJWURQJFiFOƭQKYӵc thӵc phҭPYjGѭӧc phҭm

Ĉӗng thӡi, bӝt sҩy phun còn ÿѭӧFÿiQKJLiNKҧ QăQJFKӕng oxy hóa trên thӵc phҭm thông qua bҧo quҧn lҥS[ѭӣng và xúc xích KӃt quҧ cho thҩy QӗQJÿӝ JEӝWKg nguyên OLӋXVӁFKRNKҧQăQJEҧRTXҧQWKӵFSKҭPWӕW YjFyKRҥWWtQKNKiQJR[\KyDWѭѫQJWӵ

%+7ӣQӗQJÿӝPJKg PүXWKӱ

ABSTRACT

The objectives of this thesis was researched on extraction and application of polyphenols

from rosemary (Rosmarinus officinalis) to evaluate the antioxidant activity of these

compounds Applying rosemary extraction conditions from the experimental model of

Dr Le Xuan Tien's research group to extract polyphenols from rosemary leaves on the scale of 500g of dry leaves has efficiency about 37 (% g extract/g dried feed)

Futhermore, one-factor-at-a-time method was also performed to evaluate some conditions influence of spray-dried rosemary extracts powder The best combination of conditions is used to process dry rosemary powder included a drying air inlet temperature of 140 oC, an extract concentration of 30 % (w/w), a GA/MD ratio of 3:7 (w/w), a solid - liquid ratio of 1:10 (g/mL) and a feed rate of 10 (mL/min) The powder has a polyphenol content of 61.3 (mg GAE / g powder) and the efficiency of the process

be applied in food and pharmaceutical fields

At the same time, the spray-drying powder was also assessed for the ability to prevent oxidation on food through sausage The results showed that the concentration of 2g powder/Kg material will give good food preservation ability and have antioxidant activity similar to BHT at the concentration of 200 mg/Kg sample

/Ӡ,&$0Ĉ2$1

7{L[LQFDPÿRDQWRjQEӝQӝLGXQJÿѭӧFWUuQKEj\WURQJOXұQYăQ WӕWQJKLӋSQj\OjGRFKtQKW{LOjP7ҩWFҧQӝLGXQJÿѭӧFWKDPNKҧRWURQJOXұQYăQ WӕWQJKLӋSÿmÿѭӧFWUtFKGүQFKtQK[iFYjÿҫ\ÿӫWKHRTX\ÿӏQK7{L[LQFDPÿRDQNӃWTXҧEiRFiRWURQJOXұQYăQQj\ÿѭӧFOҩ\WӯWKӵFQJKLӋP

MӨC LӨC

/ͤ,&̪0ˮ1 i

7Ï07̶7/8̴19Ă1 ii

ABSTRACT iii

'$1+0ͬ&%̪1* vii

&+˰ˮ1*7͜1*48$1 1

1.1 7͝QJTXDQY͉K˱˯QJWK̫R 1

1.1.1 *LͣLWKL͏XFKXQJ 1

1.1.2 Ĉ̿FÿL͋PWK͹FY̵W 2

1.1.3 1JX͛QJ͙FYjSKkQE͙VLQKWKiL 2

1.2 7әQJTXDQYӅFKLӃW[XҩWKѭѫQJWKҧR 3

1.2.1 7͝QJTXDQY͉FKL͇W[X̭WK˱˯QJWK̫R 3

1.2.2 7KjQKSK̯QKyDK͕F 4

1.2.3 ͰQJGͭQJ 7

1.2.4 +R̩WWtQKVLQKK͕F 7

1.3 7әQJTXDQYӅF{QJQJKӋVҩ\SKXQEӝWKѭѫQJWKҧR 12

1.3.1 *LͣLWKL͏XFKXQJY͉F{QJQJK͏V̭\SKXQ 12

1.3.2 7uQKKuQKQJKLrQFͱXF{QJQJK͏V̭\SKXQW̩RE͡WK˱˯QJWK̫R 14

1.4 7әQJTXDQYӅKѭѫQJWKҧRWURQJEҧRTXҧQWKӵFSKҭP 15

&+ѬѪ1*7+Ӵ&1*+,ӊ0 19

2.1 0өFWLrXQJKLrQFӭX 19

2.2 1JX\rQOLӋXWUDQJWKLӃWEӏWKtQJKLӋP 19

2.2.1 1JX\rQOL͏X 19

2.2.2 +RiFK̭W 20

2.2.3 'ͭQJFͭYjWUDQJWKL͇WE͓ 21

2.3 3KѭѫQJSKiSQJKLrQFӭX 21

2.3.1 3K˱˯QJSKiSÿRÿ̱͡P 21

2.3.2 ;͵OêQJX\rQOL͏X 21

2.3.3 4X\WUuQKFKL͇W[X̭WSRO\SKHQROWͳOiK˱˯QJWK̫RNK{ 22

2.3.4 3K˱˯QJSKiSÿ͓QKO˱ͫQJW͝QJSRO\SKHQRO73& E̹QJNL͋PQJKL͏PWKX͙FWK͵)ROLQ± Ciocalteu (F-C) 23

2.3.5 3K˱˯QJSKiSNK̫RViWKR̩WWtQKNKiQJR[\KRi 25

2.3.6 3K˱˯QJSKiS[iFÿ͓QKKR̩WWtQKͱFFK͇SHUR[\KyDOLSLGW͇EjRWK͵QJKL͏P0'$ 27

2.3.7 3K˱˯QJSKiSV̭\SKXQW̩RE͡WK˱˯QJWK̫R 28

2.3.8 3K˱˯QJSKiSÿiQKJLiÿ͡WDQFͯDE͡WK˱˯QJWK̫RWURQJQ˱ͣF 29

2.3.9 ĈiQKJLiV˯E͡NK̫QăQJE̫RTX̫QWK͹FSḴPWK{QJTXDFK͑V͙7%$56Yj39 29

&+ѬѪ1*.ӂ748Ҧ9¬%¬1/8Ұ1 34

3.1 &KLӃW[XҩWSRO\SKHQROWӯOiKѭѫQJWKҧR 34

3.2 .ӃWTXҧNKҧRViWVҩ\SKXQTX\P{SKzQJWKtQJKLӋP 34

.K̫RViW̫QKK˱ͧQJFͯDWͽO͏FKL͇W[X̭WWURQJO˱ͫQJU̷Q 35

.K̫RViW̫QKK˱ͧQJFͯDWͽO͏JXP$UDELFWURQJFK̭WPDQJ 37

.K̫RViW̫QKK˱ͧQJFͯDWͽO͏U̷QO͗QJ 39

.K̫RViW̫QKK˱ͧQJFͯDQKL͏Wÿ͡ÿ̯XYjR 41

.K̫RViW̫QKK˱ͧQJFͯDW͙Fÿ͡GzQJF̭SOL͏X 43

3.3 +RҥWWtQKNKiQJR[\KRiFӫDFKLӃW[XҩWKѭѫQJWKҧR 45

3.4 ĈiQKJLiVѫEӝNKҧQăQJEҧRTXҧQWKӵFSKҭPFӫDEӝWKѭѫQJWKҧR 46

&+ѬѪ1*.ӂ7/8Ұ19¬.,ӂ11*+ӎ 49

DA1+0Ө&7¬,/,ӊ87+$0.+Ҧ2 50

DANH MӨC BҦNG

%̫QJ&̭XWU~FWKjQKSK̯QKyDK͕FFKͯ\͇XFͯDFKL͇W[X̭WK˱˯QJWK̫R 5

%̫QJ6͹WKD\ÿ͝LY͉WKjQKSK̯QKyDK͕FWURQJFKL͇W[X̭WK˱˯QJWK̫RWKHRWͳQJJLDL ÿR̩QSKiWWUL͋Q 7

%̫QJ +L͏XTX̫FK͙QJR[\KyDVRYͣLFiFOR̩LJLDY͓YjWK̫RP͡F 9

%̫QJĈL͉XNL͏QNK̫RViWQKyPWK͗E̩FKW̩QJWURQJQJKLrQ FͱXFͯD0RKDPHGYj F͡QJV͹ 11

%̫QJ 7KjQKSK̯QKyDK͕FFͯDJDQYj[~F[tFK:LHQHU 17

%̫QJ 7KD\ÿ͝LJLiWU͓SHUR[LGHYj7%$56WURQJ[~F[tFKZLHQHUWURQJTXiWUuQK E̫RTX̫Qͧ o C 17

%̫QJ7KD\ÿ͝LJLiWU͓SHUR[LGHYj7%$56WURQJ[~F[tFKJDQWURQJTXiWUuQKE̫R TX̫Qͧ o C 18

%̫QJ &iFKRiFK̭WV͵GͭQJWURQJNKRiOX̵Q 20

%̫QJ 7KjQKSK̯QFͯD[~F[tFKYjO̩S[˱ͧQJ 32

%̫QJ1 Ĉ͡WDQYjÿ̱͡PFͯDE͡WWURQJNK̫RViWWͽO͏FKL͇W[X̭WWURQJO˱ͫQJU̷Q 37

%̫QJ2 Ĉ̱͡PYjÿ͡WDQFͯDE͡WWURQJNK̫RViWWͽO͏JXP$UDELFFK̭WPDQJ 39

%̫QJ3 Ĉ̱͡PYjÿ͡WDQFͯDE͡WWURQJNK̫RViWWͽO͏U̷QO͗QJ 41

%̫QJ4 Ĉ̱͡PYjÿ͡WDQFͯDE͡WWURQJNK̫RViWQKL͏Wÿ͡ÿ̯XYjR 43

%̫QJ5 Ĉ̱͡PYjÿ͡WDQFͯDE͡WWURQJNK̫RViWW͙Fÿ͡GzQJF̭SOL͏X 44

'$1+0Ө&+Î1+

Hình 1.1 +uQK̫QKFk\K˱˯QJWK̫R 1

Hình 1.2 %͡SK̵QFͯDFk\K˱˯QJWK̫R 2

Hình 1.3 0͡WV͙V̫QSḴPFͯDF{QJQJK͏V̭\SKXQ 13

Hình 2.1 1JX\rQOL͏XOiK˱˯QJWK̫RNK{ÿ˱ͫFV͵GͭQJWURQJÿ͉WjLQJKLrQFͱX 20

Hình 2.2 0i\ÿRÿ̱͡P6DWRULXV0$ 21

Hình 2.3 /iK˱˯QJWK̫RNK{VDXNKLÿ˱ͫFQJKL͉QQK͗E̹QJPi\[D\G˱ͫFOL͏X 22

Hình 2.4 Quy trìnKFKL͇W[X̭WSRO\SKHQROWͳOiK˱˯QJWK̫R 23

Hình 2.5 4X\ WUuQK ÿ͓QK O˱ͫQJ W͝QJ SRO\SKHQRO 73&  E̹QJ NL͋P QJKL͏P WKX͙F WK͵ Folin- &LRFDOWHXNL͋PQJKL͏P)-C) 24

Hình 2.6 Ĉ˱ͥQJFRQJKL͏XFKX̱QFͯDGXQJG͓FKFKX̱QDFLGJDOOLF 25

Hình 2.7 1JX\rQW̷FFͯDSK̫QͱQJJ͙FW͹GRYͣLFK̭WFK͙QJR[\KRi 25

Hình 2.8 4X\WUuQKÿiQKJLiNK̫QăQJNKiQJR[\KyD'33+ 26

Hình 2.1 4X\WUuQKÿiQKJLiNK̫QăQJͱFFK͇SHUR[\KyDOLSLGW͇EjR 28

Hình 2.10 7KL͇WE͓V̭\SKXQ/DE3ODQW6'± %DVLFTX\P{SKzQJWKtQJKL͏P 29

Hình 2.11 Quy truQKÿiQKJLiNK̫ QăQJͱFFK͇R[\KyDOLSLGWKHRFK͑V͙39 30

Hình 2.12 Quy trình ÿiQKJLiNK̫QăQJͱFFK͇R[\KyDOLSLGWKHRFK͑ V͙7%$56 31

Hình 2.13 +uQK̫QKD