Biomimetic Based Applications Part 4 ppt

Physicochemical Peculiarities of Iron Porphyrin - Containing Electrodes in Catalase - and Peroxidase - Type Biomimetic Sensors 111 2-A E Pattex Biomimetic Based Applications 112 T D Physicochemical Peculiarities of Iron Porphyrin - Containing Electrodes in Catalase - and Peroxidase - Type Biomimetic Sensors 113 Pattex T Pattex Pattex Pattex Pattex / / / Biomimetic Based Applications 114 (ƦE) (3) (4) (5) (ƦE) Physicochemical Peculiarities of Iron Porphyrin - Containing Electrodes in Catalase - and Peroxidase - Type Biomimetic Sensors 115 1 1 3 ƦE, 1 3. 4 ƦE 4 Biomimetic Based Applications 116 4. Peroxidase biomimetic sensors Ǖ = Ʀ Physicochemical Peculiarities of Iron Porphyrin - Containing Electrodes in Catalase - and Peroxidase - Type Biomimetic Sensors 117 1 2 3 (4) 5 (6) (7) 3) 1 Ǖ Ƶ Biomimetic Based Applications 118 Ǖ Ǖ Physicochemical Peculiarities of Iron Porphyrin - Containing Electrodes in Catalase - and Peroxidase - Type Biomimetic Sensors 119 Biomimetic Based Applications 120 ƦE 1 1 3 (1,1') (3, 3') [...]... clusters related to the di-iron sub-site of the H-centre of all-iron hydrogenases Chemical Communications, 847 - 848 Siegbahn, P E M.; Tye, J W & Hall, M B (2007) Computational studies of [NiFe] and [FeFe] hydrogenases Chemical Reviews, 107, 44 14- 443 5 Silakov, A.; Wenk, B.; Reijerse, E & Lubitz, W (2009) 14N HYSCORE investigation of the Hcluster of [FeFe] hydrogenase: Evidence for a nitrogen in the dithiol... proteins were well-distributed into six fractions  146 Biomimetic Based Applications Fig 2 Partial examples of rat liver proteins absorbance profiles having large band distribution difference Lane 1 is crude rat liver cytosol Lane 10 is the protein marker (Fermentas, USA) Lanes2––9show the 20 mM Glycine-NaOH buffer (pH12.0) eluates of columns A1 -4, A6, A7-56, A 84, A11-70, A17-56, A29-32, and A25-35 respectively... products of diiron compounds related to [Fe-Fe] hydrogenase: A combined experimental and density functional theory study Inorganic Chemistry, 46 , 3 84- 3 94 Cammack, R.; Frey, M & Robson, R (2001) Hydrogen as a fuel: Learning from nature, Taylor & Francis, 0 -41 5- 242 42-8, London and New York Capon, J.-F.; Ezzaher, S.; Gloaguen, F.; Pétillon, F Y.; Schollhammer, P & Talarmin, J (2008) Electrochemical insights... reduction by the [FeFe]-hydrogenases Coordination Chemistry Reviews, 253, 147 6 149 4 Capon, J.-F.; Gloaguen, F.; Schollhammer, P & Talarmin, J (2005) Catalysis of the electrochemical H2 evolution by di-iron sub-site models Coordination Chemistry Reviews, 249 , 16 64- 1676 Chiang, M.-H.; Liu, Y.-C.; Yang, S.-T & Lee, G.-H (2009) Biomimetic model featuring the NH proton and bridging hydride related to a proposed... hydrogenase: Mechanism of cyanation of a synthetic {2Fe3S} - carbonyl assembly Chemistry-A European Journal, 8, 40 37 -40 46 Gloaguen, F.; Lawrence, J D & Rauchfuss, T B (2001) Biomimetic hydrogen evolution catalyzed by an iron carbonyl thiolate Journal of the American Chemical Society, 123, 947 6- 947 7 Gloaguen, F & Rauchfuss, T B (2009) Small molecule mimics of hydrogenases: Hydrides and redox Chemical Society... Reviews, 249 , 1 641 -1652 Liu, Y.-C.; Tu, L.-K.; Yen, T.-H.; Lee, G.-H & Chiang, M.-H (2010) Submitted Liu, Y.-C.; Tu, L.-K.; Yen, T.-H.; Lee, G.-H.; Yang, S.-T & Chiang, M.-H (2010) Secondary coordination sphere interactions within the biomimetic iron azadithiolate complexes related to Fe-only hydrogenase: Dynamic measure of electron density about the Fe sites Inorganic Chemistry, 49 , 640 9- 642 0 Low,... subunit including two square-pyramidal Fe centers bridged by a dithiolate ligand, and a [4Fe4S] cluster (Fig 1) These [2Fe2S] and [4Fe4S] clusters are linked to each other via a cysteinyl thiolate on the peptide chain In addition to the Scys bridge, several cyanide and H+ e- X H2 Cys OC NC S d Fe C O S S Fep [4Fe4S] CN CO Fig 1 Protein structure of Fe-only hydrogenase from Clostridium pasteurianum (1FEH)... ion-exchange chromatography and/or hydrophobic chromatography and/or reverse-phase chromatography, which has been a powerful 142 Biomimetic Based Applications technique to simplify the proteins in sample to 2D-gel in the proteomics research(Shen et al., 20 04; Bergh et al., 2003; Jacobs et al., 20 04) One current method for the analysis of protein mixtures is proteolytic digestion followed by LC/MS/MS, often referred... lyophilized to dryness  144 Biomimetic Based Applications 2.1.6 LTQ-MS/MS analysis Redissolved peptides mixture was injected onto a Zorbax 300 SB-C18 peptide traps (Agilent Technologies, Wilmington, DE) to desalt, and separation was performed on a Zorbax 300SBC18 reverse phase capillary column (300 m inner diameter × 15 cm, Agilent Technologies) at (250 nl/min) with a linear gradient of 4- 50% B over 50 min... characterized by spectroscopy in the reaction of [Fe2( -pdt)(CO )4( 2-dppe)] with HBF4 (Ezzaher et al., 2007) Two species with hydrides located at either the all-CO or the P-substituted Fe portion are observed at the temperature below 220 K They quickly convert to the -isomer as the temperature is 128 Biomimetic Based Applications increased to above 243 K The experimental results are well consistent with the . 1,18-7) 2&<<181,>1)&apos ;4) Q166)&apos ;4) 'A&6&:7)(<)?8(:(,)816' 74) & ;4) :%1)81&apos ;4( ,)<(8)?8(:1&,)>8 74: '66(-8'?%1 84) ',2) >%19& ;4: 4):()>%( (41 ),&:8(-1,)&apos ;4) :%1)?":':&=1)A8&2-1%1'2)(=18)(@7-1,)',2)>'8A(,)':( 94) 4&,>1) :%17)'81),(:)2& ;4: &,-"& ;4% 'A61)&,)>8 74: '66(-8'?%7)BU&>(61:)1:)'6N+)DEEEG)Z',217)1:)'6N+)KFF[MN)O,) :%1)81=1 84& amp;A61)81'>:&(,+):%1)6(,1)?'&8)(<):%1)';'),&:8(-1, )4& amp;:1)&apos ;44 & ;4: 4)%1:18(67:&>)>61'='-1)(<) 9(61>"6'8)%728(-1,)&apos ;4) :%1)>':'67:&>)51)'> :4) &apos ;4) ')*1Q& ;4) '>&2&> )4& amp;:1)Bb 641 ,)1:)'6N+)KFFEMN)) ) Fe d Fe p N S S NC CO OC CN C O S Cys [4Fe4S] H + H Fe d Fe p N S S NC CO OC CN C O S Cys [4Fe4S] H + H - H ) 5&-N)KN)Z (44 &A61)&,:18912&': 14) &,)91>%',& ;49 )(<)1,;79':&>)0 K )?8(2">:&(,)A7)51#(,67) %728(-1,&apos ;41 N) O<):%1)';'),&:8(-1, )4& amp;:1)& ;4) :()&apos ;44 & ;4: ):%1)1,;79':&>)0 K )?8(2">:&(,g"?:'W1+)'='&6'A&6&:7)(<):%1) ='>',: )4& amp;:1)(,):%1),1'8A7)51)>1,:18)& ;4) ')>(9?6&91,:),1> 144 &:7)<(8):%1)%&-%)>':'67:&>):"8,(=18) <81P"1,>7)(<):%1)1,:':&> )4: ':1N)O4(6':&(,)(<):%1)9(216)>(9?61@ 14) ? (44 144 &,-):%1)';'2&:%&(6':1) A8&2- 14) %&apos ;4) A11, )4& quot;>> 144 <"667)'>>(9?6& ;4% 12)B*'Q81,>1)1:)'6N+)KFFDG)*&)I)Y'">%<" ;44 +)KFFKG)b::) 1:). 6&-',2N) bQ&,-):():%1 )4: 18&>)81?" 64& amp;(,)(<):%1)A8&2-1%1'2):%1)>(,<(8918)Q&:%)',)'?&>'6)ǔ)2(,(8)':):%1) 6 144 )%&,21812 )4& amp;21)%&apos ;4) :%1)6(Q 14: )1,18-7N)/%& ;4) 4?1>& 14) ' 64( )%&apos ;4) :%1 )49 '66 14: )'>:&=':&(,)1,18-7) <(8)8(:':&(,)(<):%1)51B$bM S )4& quot;A",&:)'9(,-)'66)& ;4( 91 84) (<)]51 K BǍ#?2:MB$bM T B$UM_N)O: )4& quot;. Ǚ)',-61):()1='6"':1):%1)>((82&,':&(,) 9(21)(<)$b)B$8'A:811)I)*'=&,+)DE[RM+)2& ;4? 6'712)&,)5&-N)`N)O:)& ;4) 21<&,12):():%1)',-61)A1:Q11,) :%1)51#51)=1>:(8)',2):%1)$b)-8("?)",218,1':%)&:N)C):7?&>'6)Ǚ)='6"1)'8(",2)DFFn)& ;4) (A418=12) Biomimetic Based Applications 126 <(8):%1):189&,'6)>'8A(,76+)Q%181&apos ;4) &:)21>81&apos ;41 4)&apos ;4) :%1)>'8A(,76 )4? & ,4) 'A(":):%1)51#51)'@& ;4) :() <(89)