Applying biofilm hybrid system (anaerobic aerobic) using coral media and microbe lift ind on automobile wastewater treatment

MINISTRY OF EDUCATION AND TRAINING HO CHI MINH CITY UNIVERSITY OF TECHNOLOGY AND EDUCATION CAPSTONE PROJECT ENVIRONMENTAL ENGINEERING TECHNOLOGY APPLYING BIOFILM HYBRID SYSTEM (ANAEROBIC - AEROBIC) USING CORAL MEDIA AND MICROBE-LIFT IND ON AUTOMOBILE WASTEWATER TREATMENT ADVISOR: Dr NGUYEN MY LINH STUDENT: NGUYEN THI KIM XUYEN NGUYEN PHUOC LOC SKL009979 Ho Chi Minh City, July 2017 HO CHI MINH CITY UNIVERSITY OF TECHNOLOGY AND EDUCATION -*** - CAPSTONE PROJECT APPLYING BIOFILM HYBRID SYSTEM (ANAEROBIC - AEROBIC) USING CORAL MEDIA AND MICROBE-LIFT IND ON AUTOMOBILE WASTEWATER TREATMENT STUDENT: NGUYEN PHUOC LOC NGUYEN THI KIM XUYEN SUPERVISOR: NGUYEN MY LINH TP Hồ Chí Minh, July/2017 SOCIALIST REPUBLUC OF VIETNAM Independence – Freedom – Happiness *** -HCM City, / /2017 MISSION OF GRADUATION THESIS Name: Nguyen Phuoc Loc Student ID: 13150132 Nguyen Thi Kim Xuyen 13150181 Major: Environmental Engineering and Technology Class: 13150CLC Supervisor: Dr Nguyen My Linh Receive date: 01/2017 Submit date: 7/2017 Topic: Applying biofilm hybrid system (Anaerobic – Aerobic) using coral media and Microbe-Lift IND on automobile wastewater treatment Fields: Research Content impletemation Design a lab-scale model of hybrid (anaerobic – aerobic) using coral media Use automobile wastewater in Mercedes-Benz company Evaluate the efficiency of nitrogen, phosphorus, COD, removal of the model during the adaptive phase and operated phase Evaluate the stability of the model after supplementation with Microbial preparation Microbe-Lift IND HEAD OF ENVIRMONMENT SUPERVISOR DEPARTMENT i SOCIALIST REPUBLIC OF VIETNAM Independence – Freedom – Happiness ******* COMMENT OF SUPERVISOR Name of student: Student ID: Major: Topic: Name of Supervisor: COMMENT Content impletemation & Amount of work: Advantage: Disadvantage: Defense: Yes/No Evaluate of kind: Score: HCM City,……/…… /2017 Supervisor (Sign & write your name) ii SOCIALIST REPUBLIC OF VIETNAM Independence – Freedom – Happiness ******* COMMENT OF REVIEWER Name of student: Student ID: Major: Topic: Name of Reviewer: COMMENT 1.Content impletemation & Amount of work: Advantage: Disadvantage: Defense: Yes/No Evaluate of kind: Score: HCM City, ……/…… /2017 Reviewer (Sign & write your name) iii ACKNOWLEDGEMENTS This thesis could not have been done without the valuable help from many people First and foremost, we want to thank my supervisor, Dr Nguyen My Linh Thanks for all your patience, understanding, help, advices, planning and encouragement throughout the whole period doing thesis graduation Thanks also for giving us the opportunity to be work with you It has been a great inspiration to learn to know your character, both in academic and human terms We would like to send many thanks to Mr Tran Kiet Huy, for providing me with the best time we have ever had at the Mercedes – Benz company Your experien-ce, opinion, view, comments and thoughts have been great and useful It was a great pleasure to be part of the laboratory of Mercedes – Benz company We want to spec-ially mention Huy to open me the doors of practical experiences and some questions gave me when we had the pleasure to discuss several times with you We really appre-ciate the high standards you have set for me, which we would never have reached without your help A special thank goes to the Ms Dong Thi Tu Anh (Dat Hop Company) and Board of Directors of Mercedes – Benz for their unfailing support We would particularly like to thank All teachers in the Department of High Quality for creating the most favorable conditions for this thesis Moreover, we would like to thank Ms Le Thi Bach Hue for supporting us equipments in laboratory was essential for the success of the thesis Last but no the least, we should say thanks from the deep of our heart to our beloved family, teachers, friends They never ending love, help, and support in so many ways through all this time Thank you so much Ho Chi Minh City, 07/2017 Nguyen Phuoc Loc Nguyen Thi Kim Xuyen iv ABSTRACT In this research, automobile wastewater treatment was performed using the Hybrid system (Anaerobic-Aerobic) with coral media The coral media is not only cheap, available but also gives high efficiency of COD, Total Kjeldahl Nitrogen, and total phosphorus removal The results were analyzed daily The Microbe-Lift IND was added in the last stage of the process to enhance the capacity of the whole system The research was conducted in two phases: adaptive and operative phase During the adaptive phase, operate for weeks to form microfilm on the coral media At the operated phase with divided into three periods corresponding to the organic loading rate of 0.25; and 2.5 kgCOD/m d In these stages, the efficiency of COD, TKN, TP treatment is high and stable With a COD removal efficiency of 74.1 ± 6.7%; 80.37 ± 12.2%; 88.1 ± 6.4% and TP were 67.35 ± 8.21%; 66.2 ± 4.3%; 58.2 ± 7.7% and TKN is 66.59 ± 9.7%; 70.81 ± 7.7%; 79.39 ± 6.2% relatively in three different organic loading rates Keywords: automobile wastewater treatment, coral media, biofilm kinetic, hybrid system… v TABLE OF CONTENTS MISSION OF GRADUATION THESIS I COMMENT OF SUPERVISOR II COMMENT OF REVIEWER III ACKNOWLEDGEMENTS IV ABSTRACT V TABLE OF CONTENTS VI LIST OF FIGURES VIII LIST OF TABLES IX LIST OF ABBREVIATIONS X PREFACE CHAPTER : INTRODUCTION 1.1 INTRODUCTION OF WASTE WATER PRODUCTION .5 1.1.1 INTRODUCE THE AUTOMOBILE INDUSTRY 1.1.2 MAIN STAGES OF WASTEWATER GENERATION 1.1.3 CURRENT MERCEDES – BENZ AUTOMOBILE WASTEWATER TREATMENT TECHNOLOGY SYSTEM DIAGRAM 1.2 INTRODUCTION TO WASTEWATER TREATMENT TECHNOLOGY 10 1.2.1 MECHANICAL METHOD 10 1.2.2 PHYSICAL AND CHEMICAL METHODS 10 1.2.3 BIOLOGICAL METHOD 10 1.3 THEORETICAL BASIS OF AEROBIC BIOLOGICAL PROCESS 10 1.3.1 AEROBIC BIOLOGICAL PROCESS 10 1.3.2 FACTORS AFFECTING AEROBIC BIOLOGICAL PROCESSES .11 1.3.3 SUSPENDED GROWTH PROCESS - ACTIVATED SLUDGE 12 1.3.4 THE GROWTH OF BIOFILM 14 1.4 THEORETICAL BASIS OF ANAEROBIC BIOLOGICAL PROCESS 17 1.4.1 ANAEROBIC BIOLOGICAL PROCESS 17 1.5 INTRODUCTION TO HYBRID TECHNOLOGY AND HYBRID TECHNOLOGY APPLICATION IN BIOLOGY WASTEWATER TREATMENT ……………………………………………………………………………… 20 1.5.1 A SUMMARY OF HYBRID BIOLOGY 20 1.5.2 THE ANAEROBIC HYBRID SYSTEM COMBINES AEROBIC 21 1.5.2.1 UANF and UAF hybrid systems 21 1.5.2.2 The hybrid system combines anaerobic and aerobic tube 21 1.5.2.3 Anaerobic and aerobic hybrid system – SAA 23 1.5.2.4 Hybrid partition system 24 1.5.2.5 MBR hybrid system combines anaerobic – aerobic 25 vi 1.6 THE REMOVAL OF NITROGEN AND PHOSPHORUS BY BIOLOGICAL METHOD 25 1.6.1 MECHANISM OF NITROGEN TREATMENT 25 1.6.2 MECHANISM OF PHOSPHORUS TREATMENT 27 CHAPTER : METHOD OF RESEARCH 29 2.1 STUDY DIAGRAM 29 2.2 MATERIAL RESEARCH 30 2.2.1 CAR MANUFACTURING WASTEWATER 30 2.2.2 ACTIVATED SLUDGE AND CORAL MEDIA 31 2.2.3 MICROBIAL PREPARATION 31 2.3 MODEL RESEARCH 33 2.3.1 MODEL DESIGN 33 2.4 SEQUENCE OF EXPERIMENT 34 2.4.1 ADAPTIVE PHASE 34 2.4.2 OPERATIVE PHASE 34 2.5 SAMPLING AND ANALYSIS 35 CHAPTER : RESULTS AND DISCUSSION 37 3.1 ADAPTIVE PHASE 37 3.2 OPERATIVE PHASE 38 3.2.1 THE CHANGE OF MLSS 38 3.2.2 REMOVAL EFFICIENCY OF COD 40 3.2.3 REMOVAL EFFICIENCY OF TP 43 3.2.4 REMOVAL EFFICIENCY OF TKN 45 3.2.5 REMOVAL EFFICIENCY OF BOD5 47 3.2.6 THE CHANGE OF PH 48 3.2.7 EFFECT OF PH TO PHOSPHORUS REMOVAL IN ANAEROBIC 51 3.2.8 EFFICIENCY OF MICROBE-LIFT IND 52 3.2.8.1 The relationship between TP decrease and Microbial preparation MicrobeLift IND 53 3.2.8.2 The relationship between microbial preparation Microbe-Lift IND and % COD 54 3.2.9 THE CHANGING OF CONCENTRATION IN ALL SAMPLING TAPS .55 CHAPTER : CONCLUSION AND FUTURE WORK 58 4.1 CONCLUSION 58 4.2 FUTURE WORK 58 REFERENCES 60 APPENDIX 62 vii LIST OF FIGURES Figure 1.1: The process of producing and assembling cars .7 Figure 1.2: Wastewater generation stages in the production phase Figure 1.3: Mercedes – Benz automobile wastewater treatment technology system diagram Figure 1.4: Biofilm construction 16 Figure 1.5: The stages of anaerobic digestion 18 Figure 1.6: Tubular Hybrid model 21 Figure 1.7: Hybrid model SAA [Y.J Chan et al., 2009] 23 Figure 1.8: Biomedical hybrid model [Y.J Chan et al., 2009] 24 Figure 1.9: Combined MBR model - Aerobic [Y.J Chan et al., 2009] 25 Figure 1.10: Schematic representation of concentration profiles for EBPR under anaerobic - aerobic conditions 28 Figure 2.1: Diagram of research method 29 Figure 2.2: Automobile wastewater 30 Figure 2.3: Coral media 31 Figure 2.4: Microbial preparation Microbe-Lift IND 32 Figure 2.5: Experimental model in drawing 33 Figure 2.6: Experiment model in practicing 33 Figure 2.7: Diagram in Operative phase 35 Figure 3.1: Coral media before and after use for treatment 38 Figure 3.2: Scanning Electron Micrographs of attached microorganism .39 Figure 3.3: The changing of MLSS in second tanks 39 Figure 3.4: Influent and effluent concentrations and removal efficiencies of COD vs time in the whole experiment (three stages) 41 Figure 3.6: The release of phosphorus in three stages 45 Figure 3.7: Influent and effluent concentrations and removal efficiencies of TKN vs time in the whole experiment 46 Figure 3.8: Influent, Anaerobic, Aerobic, Effluent concentrations and removal efficiencies of BOD5 vs time in the whole experiment 47 Figure 3.9: The change of input and output pH 49 Figure 3.10: The change of pH in Anaerobic 50 Figure 3.11: The change of pH in Aerobic 51 Figure 3.12: Relationship between pH and P release 52 Figure 3.13:The relationship between TP decrease and dose of Microbe-Lift IND 53 Figure 3.14: The relationship between %COD and dose of Microbe-Lift IND .54 viii