Nghiên cứu ảnh hưởng của ánh sáng đến sự sinh trưởng và tích lũy β carotene ở vi tảo dunaliella salina

ĐẠI HỌC ĐÀ NẴNG TRƢỜNG ĐẠI HỌC SƢ PHẠM NGUYỄN THỊ THIỀM NGHIÊN CỨU ẢNH HƢỞNG CỦA ÁNH SÁNG ĐẾN SỰ SINH TRƢỞNG VÀ TÍCH LŨY β-CAROTENE Ở VI TẢO DUNALIELLA SALINA LUẬN VĂN THẠC SĨ SINH HỌC THỰC NGHIỆM Đà Nẵng – 2022 ĐẠI HỌC ĐÀ NẴNG TRƢỜNG ĐẠI HỌC SƢ PHẠM NGUYỄN THỊ THIỀM NGHIÊN CỨU ẢNH HƢỞNG CỦA ÁNH SÁNG ĐẾN SỰ SINH TRƢỞNG VÀ TÍCH LŨY β-CAROTENE Ở VI TẢO DUNALIELLA SALINA Chuyên ngành: Sinh học thực nghiệm Mã số: 8420114 LUẬN VĂN THẠC SĨ SINH HỌC THỰC NGHIỆM Ngƣời hƣớng dẫn khoa học: TS Trịnh Đăng Mậu Đà Nẵng – 2022 C.33.44.55.54.78.65.5.43.22.2.4 22.Tai lieu Luan 66.55.77.99 van Luan an.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.C.33.44.55.54.78.655.43.22.2.4.55.22 Do an.Tai lieu Luan van Luan an Do an.Tai lieu Luan van Luan an Do an Stt.010.Mssv.BKD002ac.email.ninhd 77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77t@edu.gmail.com.vn.bkc19134.hmu.edu.vn.Stt.010.Mssv.BKD002ac.email.ninhddtt@edu.gmail.com.vn.bkc19134.hmu.edu.vn C.33.44.55.54.78.65.5.43.22.2.4 22.Tai lieu Luan 66.55.77.99 van Luan an.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.C.33.44.55.54.78.655.43.22.2.4.55.22 Do an.Tai lieu Luan van Luan an Do an.Tai lieu Luan van Luan an Do an MỤC LỤC LỜI CAM ĐOAN LỜI CẢM ƠN DANH MỤC CHỮ VIẾT TẮT DANH MỤC BẢNG BIỂU MỞ ĐẦU 1 Tính cấp thiết đề tài Mục tiêu nghiên cứu Ý nghĩa đề tài Bố cục đề tài CHƢƠNG TỔNG QUAN TÀI LIỆU 1.1 GIỚI THIỆU VỀ VI TẢO DUNALIELLA SALINA 1.1.1 Hệ thống phân loại 1.1.2 Đặc điểm hình thái 1.1.3 Đặc điểm sinh thái 1.2 Β-CAROTENE TRONG VI TẢO DUNALIELLA SALINA 1.2.1 Đặc điểm β-carotene 1.2.2 Cấu trúc β-carotene 1.2.3 Cơ chế tích lũy carotenoid Dunaliella 1.2.4 Các yếu tố ảnh hưởng đến sinh trưởng tích lũy β-carotene vi tảo Dunaliella salina 1.2.5 Vai trò β-carotene 13 1.3 ỨNG DỤNG CỦA VI TẢO DUNALIELLA SALINA TRONG ĐỜI SỐNG 14 1.4 TÌNH HÌNH NGHIÊN CỨU VỀ DUNALIELLA SALINA 15 1.4.1 Nghiên cứu giới 15 1.4.2 Nghiên cứu Việt Nam 20 CHƢƠNG ĐỐI TƢỢNG, NỘI DUNG, PHẠM VI VÀ PHƢƠNG PHÁP NGHIÊN CỨU 23 2.1 ĐỐI TƢỢNG VÀ PHẠM VI NGHIÊN CỨU 23 2.1.1 Đối tượng nghiên cứu 23 2.1.2 Phạm vi nghiên cứu 23 Stt.010.Mssv.BKD002ac.email.ninhd 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CỦA PHỔ CHIẾU SÁNG ĐẾN SINH TRƢỞNG VÀ TÍCH LŨY Β-CAROTENE Ở VI TẢO DUNALIELLA SALINA 29 3.1.1 Ảnh hưởng phổ chiếu sáng đến sinh trưởng vi tảo Dunaliella salina 29 3.1.2 Ảnh hưởng phổ chiếu sáng đến tích luỹ β-carotene vi tảo Dunaliella salina 33 3.2 ẢNH HƢỞNG CỦA CƢỜNG ĐỘ ÁNH SÁNG ĐẾN SINH TRƢỞNG VÀ TÍCH LŨY Β-CAROTENE Ở VI TẢO DUNALIELLA SALINA 36 3.2.1 Ảnh hưởng cường độ ánh sáng đến sinh trưởng vi tảo Dunaliella salina 36 3.2.2 Ảnh hưởng cường độ ánh sáng đến tích luỹ β-carotene vi tảo Dunaliella salina 39 3.3 ẢNH HƢỞNG CỦA CHU KỲ CHIẾU SÁNG ĐẾN SINH TRƢỞNG VÀ TÍCH LŨY Β-CAROTENE Ở VI TẢO DUNALIELLA SALINA 42 3.3.1 Ảnh hưởng chế độ chiếu sáng đến sinh trưởng vi tảo Dunaliella salina 42 3.3.2 Ảnh hưởng chu kỳ chiếu sáng đến khả tích lũy β-carotene vi tảo Dunaliella salina 45 KẾT LUẬN VÀ KIẾN NGHỊ 48 Kết luận 48 Kiến nghị 48 TÀI LIỆU THAM KHẢO 50 Stt.010.Mssv.BKD002ac.email.ninhd 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3.1 Tốc độ sinh trưởng trung bình vi tảo D salina phổ ánh sáng khác 29 3.2 Mật độ tế bào vi tảo D salina phổ ánh sángkhác 30 3.3 Hàm lượng caroten tích luỹ phổ ánhsáng khác 33 3.4 Năng suất β-carotene vi tảo D salina cácphổ ánh sáng khác 34 3.5 Tốc độ sinh trưởng trung bình vi tảo D salina cường độ ánh sáng khác 36 3.6 Mật độ vi tảo D salina cường độ ánhsáng khác 37 3.7 Hàm lượng β-carotene tích luỹ cườngđộ ánh sáng khác 39 3.8 Năng suất β-carotene vi tảo D salina cáccường độ ánh sáng khác 40 3.9 Tốc độ sinh trưởng trung bình vi tảo D salina chu kỳ chiếu sáng khác 42 3.10 Mật độ tảo D salina chu kỳ sáng tốikhác 43 3.11 Hàm lượng β-carotene chu kỳ chiếu sángkhác 45 3.12 Năng suất β-carotene vi tảo D salina cácchu kỳ chiếu sáng khác 46 Stt.010.Mssv.BKD002ac.email.ninhd 77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77t@edu.gmail.com.vn.bkc19134.hmu.edu.vn.Stt.010.Mssv.BKD002ac.email.ninhddtt@edu.gmail.com.vn.bkc19134.hmu.edu.vn C.33.44.55.54.78.65.5.43.22.2.4 22.Tai lieu Luan 66.55.77.99 van Luan an.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.C.33.44.55.54.78.655.43.22.2.4.55.22 Do an.Tai lieu Luan van Luan an Do an.Tai lieu Luan van Luan an Do an 54 copper availability Plant physiology, 112(2), 697–704 [22] Jahnke, L.S (1999), "Massive carotenoid accumulation in Dunaliella bardawil induced by ultraviolet-A radiation", Journal of Photochemistry and Photobiology B: Biology, 48 (1), pp 68-74 [23] Jin E.S & Melis, A (2003), “Microalgal biotechnology: Carotenoid production by the green algae Dunaliella salina,” Biotechnology and Bioprocess Engineering, vol 8, no 6, p 331 [24] Kobayashi, M., Kakizono T., Nishio, N., & Nagai, S (1992) Effects of light intensity, light quality, and illumination cycle on astaxanthin formation in a green alga, Haematococcus pluvialis Journal of Fermentation and Bioengineering, 74(1), 61–63 https://doi.org/10.1016/0922-338X(92)90271-U [25] Kobayashi, M., Kurimura, Y., & Tsuji, Y (1997) Lightindependent, astxanthin production Haematococcus pluvialis undersalt Letters, 19(6), by the stress green microalga Biotechnology 507–509 [26] Köpcke W., Krutmann J (2008), Protection from sunburn with betacarotene:a meta-analysis Photochem Photobiol [27] Kendirlioglu, Gokce, and Kadri Cetin, A (2017) "Effect of different wavelengths of light on growth, pigment content and protein amount of Chlorella vulgaris." Fresenius Environ Bull 26 : 7974-7980 [28] Katsuda, T., Lababpour, A., Shimahara, K., & Katoh, S (2004) Astaxanthin production by Haematococcus pluvialis under illumination with LEDs Enzyme and Microbial Technology, 35(1), 81–86 https://doi.org/10.1016/j.enzmictec.2004.03.016 Stt.010.Mssv.BKD002ac.email.ninhd 77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77t@edu.gmail.com.vn.bkc19134.hmu.edu.vn.Stt.010.Mssv.BKD002ac.email.ninhddtt@edu.gmail.com.vn.bkc19134.hmu.edu.vn C.33.44.55.54.78.65.5.43.22.2.4 22.Tai lieu Luan 66.55.77.99 van Luan an.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.C.33.44.55.54.78.655.43.22.2.4.55.22 Do an.Tai lieu Luan van Luan an Do an.Tai lieu Luan van Luan an 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77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77t@edu.gmail.com.vn.bkc19134.hmu.edu.vn.Stt.010.Mssv.BKD002ac.email.ninhddtt@edu.gmail.com.vn.bkc19134.hmu.edu.vn C.33.44.55.54.78.65.5.43.22.2.4 22.Tai lieu Luan 66.55.77.99 van Luan an.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.C.33.44.55.54.78.655.43.22.2.4.55.22 Do an.Tai lieu Luan van Luan an Do an.Tai lieu Luan van Luan an Do an 56 Nabila Ab G., Norhayati Y , (2021) “Photoperiod influenced the growth and antioxidative responses of Chlorella vulgaris, Isochrysis galbana, and Tetraselmis chuii” [38] Orset, S., & Young, A J (1999) Low-temperature-induced synthesis of α-carotene in the microalga Dunaliella salina (Chlorophyta) 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in different culture systems and operation regimes,” Journal of biotechnology, vol 151, no 2, p 180– 185 Stt.010.Mssv.BKD002ac.email.ninhd 77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77t@edu.gmail.com.vn.bkc19134.hmu.edu.vn.Stt.010.Mssv.BKD002ac.email.ninhddtt@edu.gmail.com.vn.bkc19134.hmu.edu.vn C.33.44.55.54.78.65.5.43.22.2.4 22.Tai lieu Luan 66.55.77.99 van Luan an.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.C.33.44.55.54.78.655.43.22.2.4.55.22 Do an.Tai lieu Luan van Luan an Do an.Tai lieu Luan van Luan an Do an 57 [45] Pisal, D S., Lele, S.S.(2005), "Carotenoid production from microalga, Dunaliella salina", Indian Journal of Biotechnology, pp 476483 [46] Rodriguez-Amaya, D B 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[60] Xi Y., Wang J., Chu J., Xue S (2000) “ Effects of different light regimes on Dunaliella salina growth and β-carotene accumulation” [61] Xu, Y., Harvey, P.J (2019) Carotenoid Production by Dunaliella salina under Red Light Antioxidants, 8, 123 [62] Ying, K., Gilmour, D.J., Shi, Y., & Zimmerman, W B (2013) Growth enhancement of Dunaliella salina by microbubble induced airlift loop bioreactor (ALB) The relation between mass transfer and growth rate Journal of Biomaterials and Nanobiotechnology, 4(2A), 1–9 Stt.010.Mssv.BKD002ac.email.ninhd 77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77t@edu.gmail.com.vn.bkc19134.hmu.edu.vn.Stt.010.Mssv.BKD002ac.email.ninhddtt@edu.gmail.com.vn.bkc19134.hmu.edu.vn C.33.44.55.54.78.65.5.43.22.2.4 22.Tai lieu Luan 66.55.77.99 van Luan an.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.C.33.44.55.54.78.655.43.22.2.4.55.22 Do an.Tai lieu Luan van Luan an Do an.Tai lieu Luan van Luan an Do an 59 [63] Zhu, Y.H., & Jiang, J.G (2008) Continuous cultivation of Dunaliella salina in photobioreactor for the production of β-carotene European Food Research and Technology, 227(3), 953–959 [64] Zamani, H., Rastegari, B And Varamini, M (2019), “Antioxidant and anticancer activity of Dunaliella salina extract and oral drug delivery potential via nano-based formulations of gum Arabic coated magnetite nanoparticles,” Journal of Drug Delivery Science and Technology, vol 54, p 101278 Stt.010.Mssv.BKD002ac.email.ninhd 77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77t@edu.gmail.com.vn.bkc19134.hmu.edu.vn.Stt.010.Mssv.BKD002ac.email.ninhddtt@edu.gmail.com.vn.bkc19134.hmu.edu.vn C.33.44.55.54.78.65.5.43.22.2.4 22.Tai lieu Luan 66.55.77.99 van Luan an.77.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.C.37.99.44.45.67.22.55.77.C.33.44.55.54.78.655.43.22.2.4.55.22 Do an.Tai lieu Luan van Luan an Do an.Tai lieu Luan van Luan an Do an Stt.010.Mssv.BKD002ac.email.ninhd 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