Nghiên cứu chế tạo hệ thống phân tích dòng chảy xách tay sử dụng các điện cực chọn lọc ion làm detector xác định các ion chứa nitơ có trong nước thải phú dưỡng

DAI HOC QUOC GIA HA NOI TRUCING DAI HOC KHOA HOC TlT NHIEN D^ T.AI: NGHIEN ClTL CHE T.AO HE THONG PH.AN TICH DONG CHAY XACH TAY Sir DUNG CAC DIEN Cl/C CHON LOG ION LAM DETECTOR XAC DINH CAC ION CHlTA NITO CO TRONG NlTOC THAI PHU DLONG MA SO : QT-97-06 CHU TRI Ot T.AI: PGS PTS PH.WI HUNG VIET ; " • — I B rf dr/ooc5i HA NOI - 1999 TfiN D^ TAI : *'Nghien cicu che tqo he thong phdn tich dong chdy xdch tay su dung cdc dien cue chon loc ion lam detector xdc dinh cdc ion chua nitatrong nuac thaiphu duffng" MA S O : QT-97-06 CHU TRI Dfi TAI: PGS PTS Phani Hung Viet, Gidm doc trung tdm Hod hoc Moi truang - Tru&ng Dai hoc Khoa hoc tu nhien - Dai hoc Quoc gia Ha Noi CACCANBOPHOIHOP: - ThS Do Phiic Qudn, Trung tdm Hod hoc Moi tru&ng, DHKHTN HN - ThS Duang Hong Anh Trung tdm Hod hoc Moi tru&ng, DHKHTN HN BAO CAO TOM TAT Detai: • Ten de tai : ''Nghien cioi che tqo he thong phdn tich dong chdy xdch tay su dung cdc dien cue chon loc ion lam detector xdc dinh cdc ion chua nita nuac thai phu duang'' • Ma so: QT-97-06 ChiJ tri de tai: • PGS.PTS Pham Hung Viet Giam doc trung tam Hoa hoc Moi truang - Truang dai hoc Khoa hpc tu nhien - Dai hoc Quoc gia Ha Npi Cac can bo tham gia: • • ThS D6 Phuc Quan Trung tam Hoa hpc Moi truang, DHKHTN HN ThS Duong Hong Anh Trung tam Hoa hoc Moi truang, DHKHTN HN Muc tieu va npi dung nghien cihi: • Muc lieu : Giai thieu mau ihiet bi phan tich dong chay su dung cac dien cue chon loc ion lam detector xac dinh cac ion nitrat nitril va amoni nuoc va nuoc thai • Noi dung : Phal tridn cac ket qua nghien cuu v^ cac loai dien cue chon loc ion dong chay su dung tai phong thi nghiem Thiet ke va che tao he thong mang thaim tich de: nang cao dp nhay, dp chon loc cua phuang phap phan tich Ung dung thiet bi de kiem ira cac mSu nuac \'a nude thai Cac ket qua dat dirac: • Nghien cuu che tao mang long chon loc ion nitral [14.15] gom c6: - % ionphore cho Nitrat (tetraoctylamonibromua) - 30% PVC - 67% o -NPOE (o-Nitrophenyioctyl ete) Dp doc dien cue 58 mV/dec khoang tuyen linh tu 10' den lO""^ M/1 va he so chon Ipc ion doi \6i cac anion gay anh huang toi qua irinh phan tich cua dien cue hau hei nho han 10" Khao sat cac dit^u kien lam \'iec cua dien cue dong chay la dem photphat 10"^ M pH = 2,8 va loc dp 2.3 ml/phiit • Che tao bp th^m tich tren ca so cac vat lieu bang nhua Teflon eo ty le dien tich be mat so vai the' tich kenh dan Idn han so vai cac bo thim tich thong thuang khae (24.6em"') (hinh 2.2) diing ket hap vcd he FIA, phuc vu cho qua trinh phan tich ion nirit • Tren ca so he FIA-ISE chpn loc ion nitrat, da khao sat cac dieu kien chuyen hoa NO -> NOx -> NO3" thong qua qua trinh trung gian chuydn hoa NOx Dung dieh chat mang la muoi KH2PO4 nong 10"^ M/1 vai pH=2,8 c6 chua 0,1% Wt H2O2 va NaN03 nong dp lO'-M/l Dung dieh axit hoa la H3PO4 nong dp 5.10'M/l Toe dp dong chat mang la 2,3ml/phut The' tich vong miu 500|il • Che tao duac dien cue chpn Ipc ion amoni mang long tren eo so ionophore nonaclin/monactin[16] bao gom: - % ionphore cho amoni (nonactin/monactin) - 30% PVC - 67% o- NPOE (octo-Nitrophenyloctylele) - KT4C1PB 70% irpng luang so vai ionophore • Khao sal cac dit^u kien lam viec ciia dien cue amoni he FIA ket hap vai bo iham tich {dong chuyen hoa NH4' -> NH, la dung dieh NaOH, pH=:12; dong chuyen hoa NH;,^ NH4* la dem Tris pH = 7,2) dt tang chpn loc ciia phep phan lich, tu ma rpng kha nang dp dung cua dien cue cho xac dinh ion amoni nuac thai Dien cue lam viec tuyen linh khoang tu 10' den lO""* mol/1 NH4^ vai dp doc la 53,2 mV/dee • Ky thuat phan lich su dung cue chpn Ipc ion la dan gian, nhanh, nhay va chinh xac cao Tren co so ket hap voi ky thuat FIA va bp tham lich cho phep tang dp chpn cac phep thir nghiem cho mot so miu thue le da dupe tien hanh va cho ket qua dang tin cay va nit ngan thai gian phan tich Tinh hinh kinh phi ciia de tai: • Tong kinh phi dupe cap la 12 irieu dong tu nguon kinh phi nghien cuu co ban ciia DH Quoc gia Ha npi di thue hien de tai nam BRIEF REPORT Title of project: '^Development of portable flow injection system with ion selective sensors detector to study nitrogen-containing ions in eutrophical wastewater^' VNU Hanoi Research grand number: as QT-97-06 Name of investigator: Ass Prof Dr Pham Hung Viet, Director, Center of Environmental Chemistry, Hanoi University of Science, VNU Hanoi Members of Project: MSe Do Phuc Quan, Center of Environmental Chemistry, Hanoi University of Science, VNU Hanoi MSe Duong Hong Anh, Center of Environmental Chemistry, Hanoi University of Science, VNU Hanoi Object and Content of Project: Object: Development of a model of portable flow injection system with ion selective sensors as detector to study nitrogen-containing ions in eutrophical wastewater Content : Development and application of liquid membrane electrode such nitrate and ammonium ion selective electrodes used as polentiometric detector in flow injection analysis system (FIA) Design and fabrication of gas dialysis chamber to enhance selectivity and sensitivity of polentiometric method used ion selective liquid membrane electrodes Application of FIA/ISE in conjunction with flow through gas dialysis chamber to determination of nitrogen-containing ions in groundwater and v.astewater in Hanoi area Results of Research Project Nitrate ion liquid membrane component: 3% Tetraocthylammonium bromide 30% High molecular PVC 67% Plaslicizer o-NTOE (oclo -nitrophenyloctylete) Dynamic slope value of the nitrate liquid membrane electrode: ^% mV/decade Dynamic range of linearity showed from 10"^ M/1 to 10'' M/1 Almost selectivity coefflcient value for foreign anions K^",, ^o be determined by mean of SSM in the range from 10" to 10^ Flow injection analysis condition: Phosphate buffer (pH=2.8, 10~ M/1) and flow rate 2.3 ml/min Design and fabrication of gas dialysis chamber from PTFE with available wider channels (a ratio surface area to volume of channel is 24.6 c m ' ) improves the efficiency of gas transfer and thus the detection capabilities of system Condition of FIA -ISE conjunction with flow though dialysis chamber for nitrite determination: Carrier solution: 10'^ M/1 phosphate buffer, pH=2.8 containing 0.1% wi H.O, and 10"' M/INO3 Oxidation solution NO/ ^ NO,: 5.10' M/l H,P04 Flow rate of carrier solution: 2.3 ml/min Sample loop volume: 500 fil Ammonium liquid membrane component: 3% nonactin/monactin 30% High molecular PVC 67% Plaslicizer o-NPOE (oeto -nitrophenyloctylete) 70 % wi/wt KT4CIPB to nonactin Investigation of working condition of ammonium electrode in FIA system conjunction with NH- dialysis chamber: Carrier solution; NaOH pH=l2 Trap solution: TRIS buffer, pH=7.2 Tins FIA system approach has been application for determination of nitrate, nitrite and ammonium in natural water and domestic wastewater Ha not, thang nam 1999 XAC NHAN CUA BAN CHU NHlfiM KHOA CHU TRI D £ TAI U ?Tl A;^^^^^^/^^^'^''''^^'^^^'""""^^*-^ XAC NHAN CUA TRUING I MO DAU Su phat trien cua khoa hpc, cong nghe va dai song hien dai doi hoi cac nha phan tich hpc phat tri^n, phdi hap nham sang tao nhung ky thual, phuang phap phan tich moi cho kha nang thue hien cac phep vdi toe dp cao, xac dinh nhung luang vet va sieu vet cac chat vai dp lap lai va dp chinh xac cang Ian [1,2] De dap ung nhimg yeu eau bat dau tir nhung nam 60 cua the ky nay, cac nha phan tich da co nhimg y tuang va co gang nghien cufu tim nhung ky thuat mcd nham tien hanh mot each ban tir dong de dong nhat cac thao tac phan tich dac biet lien hanh cac phan ung kho thue hien cac phong thi nghiem binh thuang [3-9], vi du cac phan ung tao nhimg phuc mau co dp hap thu thay doi theo thai gian, cac phan ung xuc tac can duac tien hanh didu kien nghiem ngat cac phan ung xay khong hoan loan, cac phan ung diang cac thuoc thu oxy hoa - khir khong b6n khong Mat khae, nhieu truang hap nguai la can tien hanh dong thai nhieu phan xxng phan tich mot each dong thai hoac thue hien cac phan ung noi tiep mot each nghiem ngat, sir dung cac chat che khae theo nhimg thu tu nhat dinh mot he phan ung Nhung ky thuat tren ro rang la kho thue hien dupe bang tay su dung cac dung cu thong thuang va neu phai thue hien thi tat phai nhan nhung ket qua co dp chinh xac va dp lap lai thap De phan tich nhung lucmg vet cac doi tircmg phuc tap nguai la muon ket hap cac phuang phap tach, cac phuang phap lam giau co hieu qua cao nhu chiet bang dung moi huu co, trao dOi ion vai cac phucmg phap quang hpc va dien hoa mot thiet bi phan tich boat dong tu dong [10-13] Thue hien nhung y tuang tren, cac nha phan lich da che tao duac nhung thiet bi boat dong ban tu dong ghep vai cac may quang hoac may dien hoa de phan tich nhiing truang hap rieng va sang tao nhung may phan lich hoac phuang phap mai nhu sac ky long hieu nang cao phuang phap dien di mao quan v\' Trong ban bao cao cua de lai chung toi trinh bay mot nghien cuu che tao va ung dung dien cue mang long su dung lam detector cho he FIA ket hop vai bp tham tich de phuc vu cho xac dinh mot so chi lieu ion nitrat, nitril va amoni nuac va nuac thai sinh boat cua Ha npi Nha ap dung ky thuat mai cac thao tac phan tich dupe thue hien thuan tien lap lai va nil ngdn thai gian phan lich miu Cac tin hieu phan lich thu dupe dual dang pie thuan tien cho qua trinh dinh luang n PHUONG PHAP NGHlfiN CtfU Theo nhiem vii cua da tai la nghien cuu ung dung dien cue chpn Ipc ion lam detector he thong phan tich dong chay nham xac dinh chpn Ipc cac ion chua nita moi truang, cac buac khao sat tien hanh sau day : - Che tao mang va dien cue chpn Ipc ion nitrat Che tao mang va dien cue chpn Ipc ion amoni Che tao bp tham tich sir dung mang tham teflon Khao sat cac dieu kien lam viec cua dien cue irang thai tInh va he FIA Khao sat cae dieu kien lam viec cua he thong dong chay ket hap vai bp tham tich Ung dung he thong dong chay xac dinh ham luang cua cac ion nitrat, nitril va amoni mot so miu nuac va nuac thai 2.1 Dung cu va hoa chat 2.1.1 Hoa chat Tat ca cac dung dieh d6u dupe chuan bi tu cac loai hoa chat tinh khiet phan tich (loai p.a) cua hang Fluka, Bucks, Thuy sy hoac hai loai tinh khiet hoa hpc, pha bang nuac cat hai lan Dual day la cac loai hoa chat chinh dupe su dung qua trinh nghien cuu: - Tetrahidrofuran (THE) Amoni sunphat: (NH4) 2SO4 Bac sunphat Ag2S04 Axit: H3PO4, HCl, H2SO4 axil boric, axil xitric Dung dich NaOH Cac muoi v6 co: NaCl, KH2PO4, Na3HP04, Na.PO, NaOAc, NaHCO;, NaBr Nal, NaSCN NaNO., NaNO,, NH4CI, MgCL, LiCl, NaCl, BaCU, KCl, Na,B40, KMn04 K2Cr207, H2O2 Vat lieu mang cua hang Fluka, Buchs, Thuy Sy Dung dieh dem MES (2-morpholinoethanesulfonie acid) TRIS (Tris(hydroxymethyl)aminomelhane), 2.1.2 Dung cu thi nghiem - Dien cue so sanh Ag/AgCl tiep xuc kep Hang Orion Research My Bom Mini-Micro Ismatee kenh My Van bom mau Rheodyn Model 5020, My May Digital Ion pH meter 692 Metrohm Thuy sT Bp tu ghi Kipp & Zonen BD 40 Ha lan Tliiet bi lav mau nuac mat Kemmerer, Mv 2.2 Che tao mang va dien circ chon loc ion 2.2.1 Chuan bi dung moi De hoa tan cac phan mang chung toi sit dung dung moi tetrahydrofuran khan, tinh khiet Do dac tinh cua THF co kha nang hap thu hai nuoc khong va da bi peroxit hoa didu kien binh thuang, nen THF thucmg co lin lupng nho nude va peroxit, VI vay can phai chung eat THF truac sii dung Qua trinh chung cat duac tien hanh nhu sau : Cho THF vao binh cau ba eo the tich 500 ml Cho them KOH vao binh chung cat de' lam kho loan bp nuac lan THF Dun hoi luu 1,5 gia dieu kien eo dong Nita d6 phan buy loan bp luang peroxit, sau cat lay 1/3 lupng THF ban dau thi ngung qua trinh cat THF dupe giu Ip sach, dung gia'y nhom bpc lai va dupe bao quan noi kho rao Vi THF doc va de no nen phai chung eat tu hot va iranh ngpn lira 2.2.2 Che tao mang va dien ciic Mang long chpn loc ion g6m phan chinh : phan chpn loc ion (chat irao doi ion long hoac chat tao phuc vai ion trung tinh), chat deo hoa va hcfp chat cao phan tu PVC CO long khoi luang la 200 mg Ngoai tiiy theo tijng loai mang cu tha co ih6 cho them cac phan phu nham muc dieh ue che cac ion la qua trinh cac dung dieh co cuang dp ion lan Cac phan mang duac hoa tan hoan loan bang ml THF (khan, linh khiet) mot Ip thuy linh pyrex, sau rot dung dich mang vao vong ihiiy tinh duang kinh 24 mm dai tren tam kinh sach (rua bang xa phong, nuac cat, etanol, axelon va say kho) Di cho dung moi bay hai sau 24 gia la se thu dupe mot mang long chpn loc ion CO dp day khoang 0,2 mm Diing mui khoan cat mot mang co duong kinh mm lap vao than dien cue van nang sau luyen dien cue 24 gio dung dich npi truac sir dung Tlianh phan ciia mang chpn loc ion nitrat [14.15] gom co: - % ionphore cho Nitrat (tetraoctylamonibromua) - 30% PVC - 67% o -NPOE (o-Nitrophenyloctyl ete) Thanh phan ciia mang chpn Ipc ion amoni [16] bao g6m: - % ionphore cho amoni (nonactin/monactin) 30% PVC 67% o- NPOE (octo-Nitrophenyloctylete) KT4C1PB 70% lucfng so vai ionophore lat tao k h u n g PVC (33% khoi lucmg) cue Philip ISE - 561 va tien hanh khao sat cac thong so cua dien cue dieu kien lam viec trang thai tinh va lat deo hoa DOS (66% khoi l u o n g j - h e dong chay (HA) che t a o m a n g d u a c e a n v a o t h a n dien COOR OOOR i d •- R = CH.CHaPh A - CN» - HiO A = HiO, COCR a = CN ddn u naQua-ccb\rinic \ (einv I ^ t.sier A=CM C.hdi dan ion Dicvano cnbvrinic actd-hep ta -I -t> rop v tester acia-hep takis-perchlorais '-'^/^' I of'•''''''^^! ^102^ /;7^ l.SxlO"- -2,41 • - 4.20 ').'.' 1.983 -3.34 - J.46 ; s X lO'- ! 2.')0,8 - 2.52 ; 7.2,72' - 2>MZ 4,3 - 4.23 3." ; ni th6ng i on^n '; i I 3.7 i 9.0x10-"^ 2.i : 2,2x10"' ; - - - 'i ""^ I - 4.0O i X 10" - 4,10 2,5 X 10"" i.S",2 -2.30 x 10"^ 23S.0 -3.12 1.3 253.7 - 3.S9 h x 10^ ' -> " _^ ; 1.9 X10"- -3.15 i 1.4 - 3.95 i 0.2 X ' tich (FiAl : lie dong 'Chav the cua dien cue 1 5.S i TTCI • ! Su dune cac dung; c:ch khae co pH lo vav chung toi chi su aung dien cue lam photphat >arrat MES lam chat mang I'nn hie: cho he P'^- tnnh oay n-cng hinn 3} : Toe dong cho tat ca cac phep la 12 - L4 ml/phut Day la gti m to: 'jru cua dong chay de dam iTucmg hcrp dong cung nrcmg XM nhu" a rrang thai tinh (bang 1) bao viec tiet kiem thcri gian va cd doang pic nho Thcri gian dap ung cung nnu thca gian on dinh the Tir cac ket qua khao sat cho thay co the sir dung nhanh (khoang 20s) dem MSS hoac xitrat lam char mang cho he FIA (buac nhay the tuyen tinh la - 46.0 m V/dec va -48mV/dec vung nitnt co nong nj lO'"**" -10'^ M Dung dien cue che tao duac chung toi da xac dinh nong mtnt mot so mau nuac thai song To iicn song Mhue, nude nuoi ca Qua Ker qua cho thay b) Xdc dinh he so chon loc • He so chon Ipc cua cac ion can so vol mtni uong nong MC~, mau nuac song ru CSSmg-'Ut miuc fO- Dem phot phat Ulnh Dem ME5 J Tin nicu pndn chav '.•('!! cac chat mam; ilch mini khae su dun^ Dem :^uac dien cue chon iuc •on man)^ I6nz ldm d^it-ciur irom' he !hon:^ don\:_ 70 4-8.3 rr^V/d'sc 17a - LOG n^^':~> - > - -,'_V- b V- fa; J9DT / (hj Lindane Fig.l: Flow injection analysis with photochemical destruction of DDT and Lindane Analytical c o n d i t i o n s in FIA V = 60% moi relatively of organo-chloride compounds UV-wave length : 365nm Length of reaction coil : 160cm Ingected sample volume : 50ul Carrier solution : mixture of methanol'phosphate buffer 'pK 7.8 60^rvol *-^.^ Table : Destruction efficiency of DDT and Lindane in spiked sample 10-^ 10-^ 5*10-^ 10- 10^.36 ^Q-321 5*|Q-2.66 10-2.36 Destructive effective (%) 44 67 44 42 Concentration of DDT (M/l) 10"* 5*10"* 10-3 10"^ 10^.51 10-4.12 10-3.54 10-2.56 31 30 29 26 Concentration of DDT (M/l) Sample recovery (M/l) Sample recovery (M/l) Destructive effective (%) out that certain organic solvent (e.g., methanol) are good sensitizers and may, therefore, strongly influence the photochemical destruction of the compound Photochemical destructions of organic compounds usually exhibit a much weaker temperature dependence Reactions of species in aqueous solutions have activation energies of between 10 and 30kJ moi"' Hence, a 10*^ increase (decrease) in temperature acccelerates (slows down) a reaction only by a factor of betwen 1.15 and 1.5 The relative importance of the various processes depends, of course, on the structure of the compound and on carrier solution Conclusion Ogano-chloride compounds which are destruction photochemically can be determined down to the micromole level v/ith the aid of extremely simple and economic solid state membrane electrode detector The FIA detector described for ogano-chloride compounds can be apphed also in the field of liquid chromatography where there is a need for sensitive and more specific detectors REFERENCES [1] Luong Duy Kinh, Vo Van Due, Handbook of Agricultural Chemicals, VIPESCO Vietnam, (1992) [2] Y Xu W Lorenz, G.Pfister, M Bahadir and Korte Residue analysis of triazine herbicides in soil Comparison of a ilar>' gas chromatographic and high-performance liquid chromatographic method, Fresenius Z Anal Chem325 377-380 (1986) [31 Lihana Ilcheva**, and Karl Cammann, A Simple, selective and sensitive Uquid-chromatographic or flow-injection detector for chloro-organic compounds based on ion-selective electrode* Fresenius Z Anal Chem 325 11-14 (1986) , [4] Hirokazu Haza *, Yohko Kondoh, Osamu Mitani, and Satoshi Okazaki, Solid Solvent Membrane Coated Wire Electrodes and Their Response to Nitrate, p-Toluenesulfonate Chloride, Calcium, and Potassium, Anal, Chem 62, 1139-1143 11990) [5] Emo Lindner, Klara Toth, and Emo Pungor*, Response Time Studies for Precipitate-Based Ion-Selective Electrodes in the Range of the lower Detection Limit, Anal, Chem 54,72-76(1982) [6] Jacobus F va Staden, Flow Injection Determination of Inorganic Bromide in Soils with a COated Tubular Solid-state Bomide-selective Electrode, Analyst May Vol 112,595-599(1987) [7] E m o Lindner, Klara Toth, and Emo Pungor*, Dynamic Response of Precipitate-based Ion-Selective Electrodes in the presence of Interfering Ions, Anal Chem 54, 202-207 (1982J [8] Rav-i Jain and Jerome S Schultz\ Data Analysis for Concentration Measurements in the Nonlinear Response Region of lon-Seclective Electrode, Anal Chem 56, 141-147 (1984) PHIEU DANG KY KET QUA NGHIEN CUtJ KHCN Ten de tai: "Nghien cicu che tqo he thong phdn tich dong chdy xdch tay su dung cdc dien cue chpn loc ion ldm detector xdc dinh cdc ion chua nita co nuac thdi phd dudng^^ Ma so: QT- 97 - 06 Ca quan chu tri de tai: Truang dai hoc Khoa hoc Tu nhien Ha noi Dia chi: 334 Nguyen Trai, Thanh Xuan, Ha noi Dien thoai : -n-h 84 (04) 8581419 Fax: -H-h 84 (04) 8583061 Ca quan quan ly de tai: Dai hoc Quoc Gia Ha noi Dia chi : Nha Tang, Km 8, Duong Xuan Thuy Ha noi Dien thoai : Fax : Tong kinh phi thuc chi: Trong : - Ttr ngan sach Nha nuac: - Kmh phi ci:ia tmcmg: - Vay tin dung: ^ - Von tu co: - Thu h6i: 12.000 x 1000 VND hoac x 1000 VND hoac 12.000 x 1000 VND hoac x 1000 VND hoac x 1000 VND hoac X 1000 VND hoac USD USD USD USD USD USD Thai gian nghien curu: 24 thang Thai gian bdtddu: 1/8/1997 Thai 2ian ket thuc: 1/8/1999 Ten cac can bo phoi hap nghien curu: - ThS D6 Phuc Quan, Trung tam Hoa hoc Mot truang, DHKHTN HN - ThS Duang Hong Anh Trung tam Hoa hoc Moi truang DHKHTN HN So dang ky de tai: Ngay: So chung nhan dang ky ket qua nghien cthi: Bao mat: • Ph6 bien rong rai: bien rong rdi • Pho bien han che: • Bao mat: Tom tat ket qud nghien cuu: L Nghien cihi che tao mang long chon loc ion nitrai [14.15] gom co: - % ionphore cho Nitrat (telraocTylamonibromua) - 30% PVC - 67% o -NPOE (o-Nitrophenyloctyl ere) Do doc dien cue 58 mVldec khodng tuyen tinh ii( W' den 10"^ Mil vci he so chon loc ion doi vai cdc anion gay anh huang tai qud trinh phdn tich ciia dien cue hau het nho han io-\ Khdo sdt cdc dieu kien ldm viec cua dien cue ddng chdy Id dim photphat W' VI, pH ~ 2,8 vd toe 2.3 ml/phut Che tqo bp tham tich co ty le dien tich be mat so vai the tich buSng lan han so vai cdc thiet bi thdng thuang khde {24.6cm''} tren ea sa ede vat lieu bang nhua Teflon (hinh 2.2) dung ke} hap vai he FLA, phuc vu cho qua trinh phdn tich ion nirit Tren ea sa he FIA-ISE chon Ioe ion nitrat da khdo sdt cdc dieu kien chu\en hi\2 NQz -^ NOx -^ NO J thong qua qud trinh [rung gian chuyen hod ihanh NOx - Dung dieh ehd't mang Id muoi KH-^POj nong dp 10'^ M/l vai pH~2.S dZ^ CO chua 0.1% Wi H^O: vd NaNOj nong 10'-M/l - Dung dich axil hod Id H;PO_ nong 5.I0''\VI:'I - Toe dp dong chai mang Id 2.3ml/phut - The tich vong mdu 500ul Che [go duac dien cue chon loc ion amoni mang lon-^ iren ca sa nonaetinlmonactin[\6] bao gom: - % ionphore cho amoni inonactin/monactin} - 307c PVC - 677c o- NPOE iocto-Niirophenyloctyleie) - KT4CIPB 707c luang so vai ionophore lofiophorc Khdo sdt ede dieu kien lam viec cua dien cue amoni he FLA ket hap vai bo thZim tich (ddng chuyen hod NH/ T> NHJ Id dung dich NaOH pH~l2: dong chuven hod NHj-^ NH/ Id dem Tns pH = 7.2] de tang chpn Ipc cua phep phdn itch lU nur rpng khd nang dp dung ciia dien cue cho xac dinh ion amoni [rong nuac [hai Dien cite ldm viec niyen linh khodng tu 10'' den 10'^ molll NH/ vai doc Id 53.2 niVldec Ky thuat phdn licli sic dung cue chon loc ion Id dan gian nhanh, nha\ vd chinli xdc cao Tren ca sd kei hap vai ky [luidi FIA vd bo [lid'm [leh cho phep [dug dZ> chon cac phep [hunghiem cho moi so mdu ihuc le da duac nen hanh va cho kei qua dan^ nn cdv vd rut no(Zin thZri gian phdn [ich Kien nghi ve quy mo va doi tuang dp dung nghien cicu : Ket qud nghien ciai co [he ap dun- cho cdc sd khoa hoc cong tv^he moi [ritan^i cac ca sa CO lien quan den phdn nch nuac [Unhien (nuac ngam va nuac mdtz Rien- doi vZn nuac thai sinh hoat xac dinh ion mtrat can co buac xic ly sa bo cac chat '^ax anh hu or^ den phep phdn tieh nhu cac :on haigen cdc chai humic Z\ Chu nhiem de tai Thu truang cor quan chu tri de tai HO T £ N Pham Hung Viet Chu tich Hoi dong danh gia chinh thuc Thu trirong ca quan quan Jy de tai A/^Ky' lYv Hod.h S-M/