In vivo evaluation of the ability to prevent overweight, obesity, and diabetes of 96% ethanol extraction from olax imbricata leaves

MINISTRY OF EDUCATION AND TRAINING HO CHI MINH CITY UNIVERSITY OF TECHNOLOGY AND EDUCATION FACULTY FOR HIGH QUALITY TRAINING GRADUATION THESIS FOOD TECHNOLOGY IN VIVO EVALUATION OF THE ABILITY TO PREVENT OVERWEIGHT, OBESITY, AND DIABETES OF 96% ETHANOL EXTRACTION FROM OLAX IMBRICATA LEAVES SUPERVISOR: TRINH KHANH SON VO THI NGA HUYNH NGUYEN LINH CHI STUDENT: TRAN HOANG VU SKL 0 Ho Chi Minh City, August, 2022 HO CHI MINH CITY UNIVERSITY OF TECHNOLOGY AND EDUCATION FACULTY FOR HIGH QUALITY TRAINING GRADUATION PROJECT Thesis code: 2022-17116044 IN VIVO EVALUATION OF THE ABILITY TO PREVENT OVERWEIGHT, OBESITY, AND DIABETES OF 96% ETHANOL EXTRACTION FROM OLAX IMBRICATA LEAVES Name: TRAN HOANG VU Student ID: 17116044 Major: FOOD TECHNOLOGY Advisor: TRINH KHANH SON, Assoc Prof VO THI NGA, PhD HUYNH NGUYEN LINH CHI, Eng Ho Chi Minh City, August 2022 HO CHI MINH CITY UNIVERSITY OF TECHNOLOGY AND EDUCATION FACULTY FOR HIGH QUALITY TRAINING GRADUATION PROJECT Thesis code: 2022-17116044 IN VIVO EVALUATION OF THE ABILITY TO PREVENT OVERWEIGHT, OBESITY, AND DIABETES OF 96% ETHANOL EXTRACTION FROM OLAX IMBRICATA LEAVES Name: TRAN HOANG VU Student ID: 17116044 Major: FOOD TECHNOLOGY Advisor: TRINH KHANH SON, Assoc Prof VO THI NGA, PhD HUYNH NGUYEN LINH CHI, Eng Ho Chi Minh City, August 2022 i ACKNOWLEDGEMENTS First of all, I would like to express my appreciation and sincere thanks to Assoc Prof Trinh Khanh Son, PhD Vo Thi Nga, and Eng Huynh Nguyen Linh Chi, the professors who have helped me with this thesis Beside instructing and helping me to complete my graduation thesis, the advisors have also provided me with lot of helpful advisements that myself can apply in my future and professional development In addition, I want to thank the departments and administration at Ho Chi Minh City University of Technology and Education for making it possible for me to study and complete this thesis in the best possible circumstances I also want to thank the professors at the departments of food technology and chemistry for providing me with the resources I needed to finish the thesis and for letting me use their labs Finally, I want to express my sincere gratitude to my family and friends for their unwavering support and encouragement throughout the writing of this thesis and throughout my life I have done my best, but with the limitation time of the research process and my limited knowledge, mistakens and errors are hard to advoid I appreciate your sincere suggestions to make this thesis better My sincere gratitude! Ho Chi Minh City, July 31st,2022 ii COMMITMENT I hereby declare that all content presented in this graduation thesis has been done by ourselves, including instructors and students The research content is carried out based on the requirements, design, and guidelines and is validated by the instructor The entire content of the graduation thesis has been checked against plagiarism using Turnitin software and ensures no more than 30% duplication I hereby certify that the content in the writing process is referenced in the graduation thesis from clearly sourced documents that have been correctly and fully cited in accordance with regulations Ho Chi Minh City, July 31st ,2022 iii iv v vi vii [124] T Y Wang, M Liu, P Portincasa, og D Q H Wang, “New insights into the molecular mechanism of intestinal fatty acid absorption”, Eur J Clin Invest., bd 43, nr 11, s 1203– 1223, 2013, doi: 10.1111/eci.12161 [125] K E Webb, “Intestinal absorption of protein hydrolysis products: a review.”, J Anim Sci., bd 68, nr 9, s 3011–3022, 1990, doi: 10.2527/1990.6893011x [126] T Weichhart, “Chapter Mammalian Target of Rapamycin : A Signaling Kinase”, bd 821, nr 5, s 1–14, 2013, doi: 10.1007/978-1-61779-430-8 [127] M A Welte, “Expanding roles for lipid droplets”, Curr Biol., bd 25, nr 11, s R470– R481, 2015, doi: 10.1016/j.cub.2015.04.004 [128] G Wilcox, “Insulin and insulin resistance”, Aliment Pharmacol Ther Suppl., bd 22, nr 2, s 61–63, 2005, doi: 10.1111/j.1365-2036.2005.02599.x [129] T William‐Olsson, “Α‐Glucosidase Inhibition in Obesity”, Acta Med Scand., bd 219, nr 706 S, s 1–39, 1986, doi: 10.1111/j.0954-6820.1986.tb19118.x [130] D P Xu m.fl., “Natural antioxidants in foods and medicinal plants: Extraction, assessment and resources”, Int J Mol Sci., bd 18, nr 1, s 20–31, 2017, doi: 10.3390/ijms18010096 [131] F S Yen, J C C Wei, M C Lin, C C Hsu, og C M Hwu, “Long-term outcomes of adding alpha-glucosidase inhibitors in insulin-treated patients with type diabetes”, BMC Endocr Disord., bd 21, nr 1, s 1–10, 2021, doi: 10.1186/s12902-021-00690-0 [132] W Yin m.fl., “Plasma lipid profiling across species for the identification of optimal animal models of human dyslipidemia”, J Lipid Res., bd 53, nr 1, s 51–65, 2012, doi: 10.1194/jlr.M019927 [133] J Yu og P Li, “The size matters: regulation of lipid storage by lipid droplet dynamics”, Sci China Life Sci., bd 60, nr 1, s 46–56, 2017, doi: 10.1007/s11427-016-0322-x [134] C Zhang, H Li, og R Han, “An open-source video tracking system for mouse locomotor activity analysis”, BMC Res Notes, bd 13, nr 1, s 48, 2020, doi: 10.1186/s13104-0204916-6 [135] Q W Zhang, L G Lin, og W C Ye, “Techniques for extraction and isolation of natural products: A comprehensive review”, Chinese Med (United Kingdom), bd 13, nr 1, s 1–26, 2018, doi: 10.1186/s13020-018-0177-x 63 APPENDICES Appendix 1: Results of one-way ANOVA analysis of body mass of mouse groups in 12 weeks of in vivo testing Week 10 11 12 ND 15.49±0.15 16.62±0.63 17.80±0.84 19.66±0.30 21.97±0.55 23.24±0.56a 24.47±0.63a 25.60±0.65a 25.83±0.57a 26.27±0.46a 26.70±0.42a 27.17±0.45a 27.58±0.38a HFD 20.00±0.49 22.61±0.52 24.57±0.60 25.68±0.49 28.33±1.37b 30.45±1.26b 32.03±1.01b 33.55±0.79c 35.03±1.05c 36.07±1.26c 36.66±1.10c 37.23±0.85c HFE-50 26.17±0.45ab 27.44±0.57ab 28.94±1.06ab 30.17±1.34bc 31.03±1.33bc 31.82±1.21b 32.37±1.01b 32.90±1.13b HFE-100 25.40±0.44ab 26.94±0.50a 27.88±0.45a 28.69±0.61ab 29.17±0.57ab 29.51±0.51ab 30.06±0.57ab 30.51±0.64ab HFE-200 25.09±0.29ab 26.59±0.20a 27.26±0.37a 28.20±0.37ab 28.56±0.42ab 28.94±0.46ab 29.36±0.47ab 29.91±0.49ab HFA 25.09±1.06ab 26.43±1.00a 27.56±1.29a 28.59±1.33ab 29.11±1.31ab 29.87±1.29ab 30.41±1.32ab 30.95±1.28ab Note: Different letter values in the same row indicate a statistically significant difference (p