Tiêu chuẩn iso 03401 1991 scan

I SO I N TE RN ATI ON AL S TAN D ARD 340 Se co n d ed i ti on 991 - 2- 01 Ci g arettes by th e Ci g aret t es W- e s - - fi l ters - Determi n ati on - Spectrometri c D&t ermi n at i o n M Bt h o d e de la rkt en t i on of al kal oi d reten ti on met h o d d es al cal oi ’ d e s par /es s pe ct ro m&i q u e Re f e re n ce I S0 n u m be r 40 : 9 ( E) I S0 3401 : 991 ( E) Foreword I S0 ( th e i n tern ati on al fed erati on of of prepari n g tech n i cal wh i ch I n tern ati on al tech n i cal represen t ed men t al an d ( I EC) to a Th i s Al l or Tobacco I S0 i n terested of for by by at in th e l east worl d wi d e Th e I SO, al so a I S0 su bj ect th e ri g h t for to be g overn - t ake part in the El ectrotech n i cal stan d ard i zati on tech n i cal commi t t ees Pu bl i cati on 75 % work t h rou g h org an i zati on s, I n tern ati on al voti n g ou t h as el ectrotech n i cal ad opt ed bod i es wi th the a of th e as an are I n tern a- membe r bod i es I S0 an d wh i ch 401 was t obacco can cel s h as prepared an d been by Tech n i cal Commi t t ee pro d u ct s repl aces tech n i cal l y th e fi rst edi ti on revi sed 991 reserved an y mean s, permi ss i on In Case In No Post al e part el ect ron i c wrl tl n g I n tern ati on al Prl n t ed carri ed establ i sh ed l i son wi th approval ed i ti on 3401 : 977) , ri g h ts by St an d ard 26, secon d ( I S0 req u i res bod y is bod i es) vote I n tern ati on al I SO/TC me mbe r me mbe r n ormal l y been in mat t ers St an d ard s the St an d ard casti n g al l ( I S0 I n tern ati on al cl osel y on I n tern ati on al ci rcu l ated h as n on - g overn men t al , Stan d ard i zati on ) is me mber commi t t ee col l aborates Commi ss i on ti on al Each th at for bod i es St an d ard s commi t t ee on I S0 Draft st an d ard s commi t t ees a work Org an i zati on n ati on al f rom of or the Org an i zat i on 56 Swl t zerl an d l CH - 21 th l s pu bl l cat l on mech an i cal , m ay I n cl u d i n g be pu bl l sh er for St an d ard i zat i on Gen t &e 20 l reprod u ced ph ot ocopyi n g Swi t zerl an d or u ti l i zed an d In mi crofi l m, an y f orm wi th ou t I N TERN ATI ON AL C i g arettes Det ermi n at i on - - fi l ters I S0 S TAN D ARD Spect romet of al kal oi d ret en t i on I S0 3308: 991 , mach i n e I n tern ati on al St an d ard speci fi es t wo the spectrometri c d etermi n at i on of al kal oi d by fi l ters of Rou t i n e an al yt i cal Defi n i t i on s an d ci g aret t e- smo ki n g s t an d ard d i t i on s 40 : 9 , Ci g aret t es - Det ermi n at i on of al ka- rel oi ds ten ti on - met h od s I S0 for the me t h o d ri c Sco pe Th i s by 401 : 991 ( E) in s mo ke co n d e n s at e s - Spect romet ri c ci g arettes: met h od - th e di rect met h od ; I S0 - th e Th e i n di rect met h od s di rect bl e owi n g s o me th e of fi l ter is Th es e t obacco reten ti on of oth er of n ot Th e appl i ca- ret n ed exampl e, i n di rect wi th Th i s th e is th e Th e al - met h od perforat ed of an d fi l ters f or I S0 43 7: 9 , an d n i cot i n e- free ti ne an al yt i cal I S0 43 : 9 , an d Ci g aret t es d ry t obacco an d d i t i on i n g - - Det ermi n at i on part i cu l at e s mo ki n g pro d u ct s At - t est i n g mat t er of total a u si n g ro u - mach i n e wi th I n tern ati on al case reten ti on Ci g aret t es - Sampl i n g or St an h avi n g eq u i pped Defi n i ti on For the wi th wrappi n g met h od s of i s n ot in porou s al kal oi d s smo ke fi l ters) wraps ci g arettes it (for ci g arettes n i coti n e or N OTE recovery appl i cabl e i rreversi bl e fi l ter u n l ess materi al ch arcoal to to u sed fi l ter ti ppi n g n ot perforat ed be i n compl ete appl i cabl e porou s an to t ypes appl i cabl e sh al l f rom n ot d ard are met h od kal oi d s is met h od Tobacco 40 : 9 , mo s p h e r e d et ermi n e smoke, su bst an ces n ecessari l y th e reten ti on expressed presen t rel ated to as in th e on l y n i coti n e the of of d efi n i ti on th i s I n tern ati on al St an d ard , th e appl i es Th e mai n st ream al kal oi d pu rposes fol l owi n g reten ti on al kal oi d Th e rati o, ret en t i on expressed the al kal oi d s th e fi l ter: retai n ed i n d ex as a by R, , , of a ci g aret t e percen t ag e th e fi l ter to by t h ose fi l ter: mass , of en teri n g Gc Rn i , = - x 00 sn i c N ormat i ve ref eren ces B Th e fol l owi n g th rou g h of st an d ard s referen ce th i s in I n tern ati on al cati on , th e edi ti on s d ard s are su bj ect ag reemen t s are bas ed en cou rag ed pl yi n g th e di cated reg i sters most bel ow of to t n thi s to sti tu te At i n d i cated revi si on , on th i s th e val i d of of provi si on s val i d an d I EC an d I n tern ati on al of pu bl i - Al l stan - parti es to Th e al kal oi d reten ti on i n d ex may be d et ermi n ed as fol l ows: St an d ard possi bi l i ty the ‘ S” k wh i ch , ti me I n tern ati on al ed i ti on s Members the were i n vesti g ate recen t cu rren tl y text, St an d ard provi si on s I S” k of ap- st an d ard s I S0 i n- mai n t n St an d ard s a) Di rect /y, l oi ds by meas u remen t retai n ed kal oi d s in th e by th e fi l ter mai n st ream of the an d of mas s the s mo ke of mas s l eavi n g al kaof al the fi l ter: I S0 3401 : 991 ( E) Fn l c Rn l c = I ;, ic H n i c+ Fn l c ' l oo = Sn , , ' I n di rect met h o d l oo Smoki n g an ce wh ere wi th of I S0 th e ci g arett e- smoki n g is Fn i c th e th e mas s of al kal oi d s retai n ed by q u i remen t s st ream fi l ter; H is ni t th e mas s st ream S is nl c the fi l ter of al kal oi ds s mo ke mas s [ see l eavi n g of ( A) in the al kal oi d s the mai n - fi l ter; en t eri n g n at i on I n di rectl y, t ween by the mai n st ream ( A) an d 2 l en g th a of ci g arette mas s materi al wh i ch d i fferen ce wi th from s ame the ti tter an ot h er removed i s th e be- in ( B) , as th at the of the - l -l , i c I S0 accord - an al yti cal of the th e preparat i on d en sat e ten t by re- mai n of an d a d etermi - di sti l l ati on in ac- 400 of the remai n i n g I S0 43 87 on mach i n e I S0 th e fi l ter fi l ter t obacco a rou ti n e of by an d f rom ( A) , in an al yti cal of in s econ d req u i remen t s mai n st ream a of wi th ci g arette- smoki n g met h an ol i c d et ermi n ati on di sti l l ati on a smoki n g accord an ce th e th e preparati on d en sat e ( B) wi th col l ecti on d en sat e, materi al ci g arettes rod s compl yi n g 33 08, the of i d en ti cal sol u ti on of accord an ce of s mo ke i ts wi th of al kal oi d I S0 3400 F”i C x s = al kal oi d Remo val ten t ( A) : Sn i t Rn i c f rom fi l ter ci g arette th e t n ed correspon d i n g wi th s mo ked fi l ter th e of al kal oi d s s mo ke of ci g arette of th e in wi th col l ecti on d en sat e, of ( A) rou ti n e the above] measu remen t mas s i ts wi th a compl yi n g 308, sol u ti on of sampl e b) I S0 s mo ke cord an ce ci g arettes on mach i n e of met h an ol i c fi l ter 43 87 00 = s, i , x 00 Reag en t s nl c Use wh ere on l y an d is Nn l c th e mas s st ream fi l ter is Lq n i c in the the mai n - ci g arette the mas s of al kal oi d s s mo ke fi l ter from materi al in th e the Di rect Smo ki n g of 43 87 smoki n g I S0 after aci d the fi l ter on bu tts removal wi th to in accord an ce ci g arette- th e the to remo ve an d ( A) , Met h an ol Sod i u m of by of th e of by th e n eu tral ti ps of st eam from the add i ti on al kal oi d s; of from of from fi l ter n ati on of cord an ce wi th al kal i , the al kal oi d th e th e fi l ter of aci d the h yd roxi d e, sol u ti on , c( N aOH ) = Su l fu ri c aci d , sol u ti on , c( H , SO, ) = Su l fu ri c aci d , sol u ti on , c( H $OJ = 0, 025 5 Ni coti n e, mi n i mu m pu ri ty 98 mol /l mol /l % Apparat u s U su al an d th e of I S0 th e al kal i n e t en t di sti l l ati on the fol l owi n g i tems: Con d i t i on i n g an ce wi th th e en cl osu re, req u i remen t s reg u l at ed of I S0 in accord - 3402 Rou t i n e an al ytl cal ci g arett e- smoki n g compl yi n g wi th th e g l ass fi l ter s mo ke fi bre req u i remen t s trap ( see of I S0 mach i n e, I S0 3308, wi th 4387) ten t an d an d di sti l l ati on St eam fol l owi n g d en sat e preparati on d en sat e an d absorban ce n i coti n e s mo ke by ch amber di sti l l ati on , as ( A) , t en t 3400 th e apparat u s ti ps, f rom st eam- al kal oi d of mai n st ream al kal oi d st eam l aboratory di sti l l ate di sti l l ati on of ci g arettes sol u ti on i ts th e esti mati on the Col l ecti on met h an ol i c of in meas u remen t di sti l l ate the g rad e eq u i val en t the di sti l l ati on an d d i scard i n g resi d u e spect rometri c cal cu l ati on l east al kal i n e an al yti cal at mol /l req u i remen t s fi l ter su bj ecti on met h an ol , su bst an ces, Ren d eri n g of pu ri ty ( B) an al yti cal wi th of remai n i n g ; of sol u ti on vol ati l e rou ti n e compl yi n g an d ad di ti on ci g arettes a mach i n e 3308, ci g arette recog n i zed wat er met h od wi th I S0 or mai n - ci g arette removed of wat er wi th Pri n ci pl e of al kal oi d s from ( A) : st ream th e of s mo ke reag en t s di sti l l ed of apparatu s, si sti n g of th e Di sti l l ati on ch amber a d et ermi in di sti l l ati on parts: ac- A of cyl i n dri cal , abou t i n l et at 50 i ts verti cal l y ml to base 00 mou n t ed ml capaci ty, di sti l l ati on wh i ch h as ch amber a s t eam I S0 It sh al l be mai n tai n possi bl e a to h eat stan t th e l i qu i d ch amber l evel in d u ri n g ord er the to di sti l - Spectromet er, from 23 nm to coveri n g 90 a 401 : 991 ( E) wavel en g t h ran g e n m l ati on 6 Di sti l l ati on spl ash h ead Qu art z cm, or si n g l e 3 J acket ed fi tti ng on of to coi l the Pl u g - t ype sod i u m fi l ter d en ser, di sti l l ati on fu n n el , h yd roxi d e wi th spl ash or oth er sol u ti on sph eri cal h ead if for ad d i ti on req u i red , be am absorban ce an d after recti on 6 of th e di sti l l ati on th e syst em proced u re ( 5) be at at by rou ti n e brated N OTE 98 it Th e al so be to % of wi t h th e 3) u sed is of g i ven provi d ed to n i coti n e d i ag rams are the of as th e l en g t h case of of a sh al l of the cel l s sh al l measu remen t : be if be n ot, eq u al a before su i tabl e cor- appl i ed fl asks, of capaci ty 250 ml , wi th stoppers 25 On e- mark pi pettes, of capaci ti es ml , ml or ml sol u ti on If di sti l l ati on sh al l n ot, rate n i coti n e sal t cu rren t l y u sed op- Gl ass Fi l ter fu n n el s, of d i amet er abou t 55 mm For cal i - paper, fast fi l teri ng g rad e ( 5) apparatu s the i n d i cated Recovery val u e u se exampl es that th e n i coti n e l evel th eoreti cal possi bl e pu re wi th pu re expect ed mod i fi cati on tests 3400) path in apparatu s accord an ce maxi mu m ag n st u res same ( I S0 the l east ti mi ze in each Vol u metri c Test opti cal cel l s apparat u s Th e g rou n d Test i n g an mat ch ed th e ti ps h avi n g j oi nt ( 2) s yst em an d , cel l s, i den ti cal Ot h er resu l ts apparat u s obtai n ed ( fi g - Sampl i n g m ay are th e Carry ou t speci fi ed sampl i n g in I S0 in accord an ce wi th the met h od 8243 I S0 40 : 9 ( E) Di m e n si o n s stopper Th orpecon d en ser Fron t vi e w N OTE S Al l g l ass Sto pco cks: is med i u m mm wal l bore borosi l i cate P TFE Fi g u re - Exampl e of apparat u s cu rren tl y in u se in mi l l i me tre s I S0 3401 : 991 ( E) Dl me n si on s In mi l l i met res ,.ii i Ai 032 33 ; , -’ - \* ,d /4 i El ect rl cn l Fi g u re h eat l n g - Exampl e of apparat u s cu rren t l y in u se I S0 401 : 991 ( E) Di men s i on s 60 t- Ad apter tar the Ll q u l d s ampl e ( RS2 R CN 29 ’ ext 65 84 R CN 24 , St an d ard d en scran d dl stl l l ate recei ver L Ol sch arg e N OTE - Al l g l ass is med i u m wal l W borosi l i cate Fi g u re - Exampl e of apparat u s cu rren tl y in u se f In ml l l i met res I S0 En su re Pro ce d u re the th at the di sti l l ate to sol u ti on Di rect met h o d ( 9) , is Preparat i on of test sampl e Sel ect from the cord an ce wi th ci g arettes for l aboratory cl au se the s ampl e th k set of to retai n ed I S0 prepared in req u i red ’ n u mber smoki n g ru n s of to be n ot in in an d th e d i ti on t h em d i ti on i n g in accord an ce en cl osu re wi th I S0 wi th ci g arett e- smoki n g smoki n g in process smoked At th e ter to the en d ti ps of an d wi th ou t as req u i red each th e d el ay an al yti cal on e or for mo re smoki n g h as been exti n g u i sh process to s mo ke each ci g arette Proceed accord - mai n st ream l en g th , smoki n g an y in trap bu tt traps at th e each in rou ti n e ( 2) appropri ate s oon ( ) the Col l ect the As on mach i n e processes d en sat e ci g arettes 43 87 ci g arette fi l ters of remove 2 th e an d fi l - Carefu l l y retai n ed in met h an ol a an d cu t cord i n g maxi mu m an y to the of th ree sti l l an d ad h eri n g open ti ps met h an ol ( ) i n to t obacco Open in th e the from sti l l th e an d di sti l l ati on i n trod u ce the A di rectl y i n to extract, as i n a fl ask an d i n n er t h ese an h fi l - abou t to al tern ati ve th e di sti l l ati on by th e it ch amber Pl ace ti ps for mi n h an d U si n g di sti l l ati on as the sod i u m Take h yd roxi d e th e in ml beaker t u be tai n i n g En su re or aci d Sl owl y h yd roxi d e cl osi n g ml of th e pl aci n g the ti ps 00 ml of met h an ol i t to st an d overn i g h t tran sfer of ml ex- th e di sti l l ati on mo ve sti l l , ti ps a an d i nl et fl ask water u si n g 50 aci d I n di rect 00 ( 2) fu n n el the to to th e is ad d th e ml to the of an d l et it sh ake st an d I S0 for di si n teg rate th i s sol u ti on mai n st ream wi th t wo an d to s mo ke 3400 met h o d Sel ect from cord an ce ci g arettes ti on the wi th of in s ampl e l aboratory cl au se req u i red t h em test sampl e for twi ce the accord an ce prepared th e di rect wi th met h od I S0 in n u mber 40 of ac- fi l ter an d d i - th e d i - in en cl osu re i n to of th e of l ast (5 3) in di sti l l ate remove the total su b- sampl es, X prepared an d Y the t u be cl ean the the a ti ppi n g n ew g i n al fi l ter Y, sl eeve ti ppi n g ti ppi n g materi al l eavi n g h as sl eeve on the th e to of be of the ti ppi n g ci g arettes sl eeve removed , the s ame in repl ace l en g th from pl ace as If i t wi th th e ori - ci g arette 2 Det erml n at l on th e sod i u m d rops an d Separat e i den ti cal - ch amber, d el i very ( ) t wo d e- ( 6) sol u ti on th e th e remove i mmers ed ml start di sti l l ate I n sert di sti l l ati on n ecessary an d the di sti l l ati on as 230 of fl ask aci d t u be ri n si n g Termi n at e if di sti l l ate ( 4) ml wh i l st ml ( 3) di sti l l ati on, vol u metri c su l fu ri c of 20 the th e ml carefu l l y forceps the fl ask n ot al i qu ot in accord an ce Preparat i on Re mo ve sol u ti on abou t the en d Col l ect the l i ttl e d i scard i n to of an wi th forceps, wh i l e ad d ed st oppi n g an d sol u ti on th e th rou g h su l fu ri c the porti on , al kal oi d s fron t or d oes ml ) pl ace i s to u se a met h an ol i c al l ow d u ri n g bei n g Col l ect ml th at d i sc th e mi n ci g - ch amber to an d a pi pette care Wi t h ou t beaker l i very the 20 th ree met h an ol i c 20 wi th ac- ci g arettes to of h ol d er Cl ose for an d t wo h el d of ad j u st ed ( i deal l y th e ca- vol u me al i qu ot d i sc fol d of ch amber is th e di sti l l ati on a th e a of of fl ask th at in traps ri n g an d fl ask be from the fi l ter seal i n g i cal di sti l l ati on sh aker test su b- sampl e Ad d th e th e the d en sat e in su rface i n to of met h od : forceps sh al l mai n - ci g arettes su i tabl e al kal oi ds bl an k en su ri n g s ampl e - a el ectri cal ti on i n g WARN I N G for a a n u mber an y wi th in th e fi l ter ch amber d esi rabl e fol l ows: Sh ake sh ake tract l ess wi th th e fol l owi n g remo ve vol u me th e th e the d i sc d i sc t n ed of to th e Th e the t aken th e N OTE the al kal oi d s accord an ce tai n i n g tai n i n g th at are q u arters Cl ose U se the in f rom an d fol d ed ml so arettes U se, t h em the paper (I -I , , , ) traps h ol d er (5 ) s mo ked th e ( I &) remove ti ps fi l trate in al kal oi d s accord i n g the 50 Wi pe the Remo ve Pl ace paci ty pu t al kal oi ds the forceps d et ermi n e ter of d en sat e proceed sol u ti on i t stag e Det ermi n at i on of fi l ters, d en sat e removed s mo ke twi ce on 2 ci g arette s moke Di sman t l e I S0 ml If fi l ter 402 wi th an ce 50 a spectrometri cal l y Det ermi n at i on s moked , d i ti on ed t h rou g h di l u te mi x ( ) Smo ki n g th e an d carri ed Det ermi n at i on Smo ke fi rst temperatu re, wat er ac- fi l ter H avi n g ro om wi th 3400 st ream the th e at fi l ter d et ermi n e mai n st ream ou t is mark cl ear, d i scard i n g sol u ti on fl ask th e 401 : 991 ( E) pass 2 Det ermi n at i on mai n st ream ci g arettes s moke of of al kal oi d s d en sat e su b- sampl e X of in th e th e fi l ter (I ! & Rewi th th e fi l ter Smo ke wi th s moki n g on e th e I S0 or ci g arettes 43 87 on mach i n e more of th e ( 2) smoki n g su b- sampl e rou ti n e to the ru n s X in accord an ce an al yti cal ci g arette- req u i red an d bu tt col l ect l en g th th e in mai n - , S O 40 : 9 ( E) st ream i ng s mo ke d en sat e in a trap for each s mok- Di rect met h od ru n Re mo ve th e traps accord an ce an d wi th t i n u e the d etermi n at i on in Fn i c J I ”i C Det ermi n at i on 2 mai n st ream s mo ke ci g arettes Smo ke way of the as of al kal oi d s d en sat e su b- sampl e ci g arettes t h ose of of of the ci g arette h ol d er of the s moki n g su b- sampl es Y an d in of n on - fi l ter X, proj ecti n g in from mach i n e the Y the en su ri n g is Col l ect pare as the th e a s ame mai n st ream met h an ol i c d escri bed ti ppi n g th e is th e th e l en g th sl eeve as bot h 2 , to the s ame of sn i c the R”i C d escri bed of the in in S”, , rod Expressi on th e an d Express the ad d i n g sol u ti on the d en sat e per sol - al l ten t, expressi on Met h od Cal cu l ate st ream per of s mo ke of th e an d al kal oi d d en sat e, ci g arette, accord an ce cal cu l ati on for wi th each I S0 fi l ter al kal oi d resu l ts ten t of expressed in the smoki n g ru n , Cal cu l ate s mo ke ru n s me an set as of the smoki n g mean al kal oi d d en sat e of ru n s Cal cu l ate mai n st ream th e in sampl e as smoki n g th e s mo ke th e t en t per resu l ts set of obt n ed accord an ce mean wi th al kal oi d d en sat e me an ci g arette wi th of smo ke th e th e as d esi rabl e to of th e al kal oi d mean of as n i coti n e, the cal cu l ate mg of in for the mi l l i - n earest test sampl e n earest fi d en ce reten ti on the sampl e; the th e the the test of to fi l ter, to the i n d ex percen t ag e, 0, Ol sampl e; smoked , fi l ters reten ti on a n earest test by of mi l l i g rams % It i n terval i n d ex of th e smoki n g for I S0 each 3400 Test 1 an d th e t en t for resu l ts th e of wh ol e obt n ed the Th e th e al kal oi d by each t en t the fi l ter, smoki n g in of each s mo ke mi l l i g rams ru n in report sh al l obt n ed d i ti on s St an d ard , n ot or sh ow It th at as may met h o d al so speci fi ed reg ard ed ci rcu mst an ces th e sh al l in thi s opti on al , h ave u s ed men t i on an y I n tern a- as wel l as th e re- i n fl u en ced su l ts test for the test resu l ts operat i n g ti on al report Th e test report of test sh al l i n cl u d e i den ti fi cati on report shal l , i n formati on l i sted of in in al l th e detai l s parti cu l ar, to req u i red for sampl e i n cl u d e th e i t ems I S0 3402 per accord an ce Descri pti on Sampl i n g of th e prod u ct tested 3400 th e d en sat e of the fi l ters resu l ts Th e pressed of for Cal cu l ate wh ol e of th e retai n ed fi l ter, I S0 al kal oi d compl et e Cal cu l ate the is Th e al l in in ru n d en sat e ret n ed fi l ters, an y th e th e ci g arette for n i coti n e, 3400 th e mai n st ream to of expressed of mai n - mi l l i g rams smoked , per as d en sat e, ci g arettes ten t, mg me an cl IO fol l ows: expressed d en sat e g rams formu l ae i n di vi du al as s moke ci g arette the 0, Ol resu l ts resu l ts mai n st ream 2 an d test al kal oi d Det ermi n e smo ke Cal cu l at i on of d en sat e an d s mo ke pre- b) 00 l fni c bot h t obacco s mo ke separat i n g of - = ci g arette d en sat e met h an ol i c ten t x met h od su b- sampl es s mo ke sol u ti on in al kal oi d u ti on for I n di rect s ame th at s mo ked 00 Kl c th e th e su b- sampl e th at + ( A’ “, , ) su b- sampl e l en g th x Rn i c= as test a th e for al kal oi d percen t ag e sampl e al kal oi d retai n ed of obtai n ed me an mean is eq u al by test mass , of fi l ter as smoki n g reten ti on to th e sampl e each by t en t the al l &, , the a) met h od of sampl i n g ; b) n u mber of ci g arettes c) d at e me an ru n i n d ex of for the proced u re: the an d pl ace of in th e pu rch ase test or sampl e; sampl i n g exfi l ters Test d i ti on s, in accord an ce wi th I S0 Test resu l ts, expressed in accord an ce wi th I O 52 I f obtai n ed by th e i n di rect 3401 : 991 ( E) met h od : I f obtai n ed compl et e by a) the b) al kal oi d ten t n i coti n e per ate val u es test the Fn i , , to each expressed th e in n earest mi l l i g rams d) ten t n i coti n e per val u es for to each al kal oi d ten t per 0, Ol i n di vi du al mg ; al kal oi d t en t n i coti n e per set ZI ”i , , to each th e in n earest of ru n , th e n earest i n di vi d u al mi l l i g rams 0, Ol smoki n g expressed of ten t n i coti n e per d en sat e, val u es for N, i , , mg ; of separ- in mi l l i g rams 0, Ol mg ; smoki n g ru n , of d) s mo ke from mg ; to each th e set of th e n earest of in 0, Ol mg ; smoki n g t en t n i coti n e per al kal oi d th e reten ti on n earest smoki n g %; i n d ex &, , separat e of e) s mo ke as a from mg ; 0, Ol each set al kal oi d ten t n i coti n e per of h) referen ce fi l ter ci g arettes, val u es for of s mo ke to each the i n di - avai l abl e; expressed of th e th e in mi l l i g rams mai n st ream fi l ter separat e S, i , , ci g arettes, val u es expressed ci g arette, from 0, Ol mg ; smoki n g al kal oi d ten t per of th e for of s mo ke to each if S, , i , , f rom mg ; th e in the set mi l l i g rams mai n st ream fi l terl ess of ci g arettes, val u es for of s mo ke to each the i n di - avai l abl e; expressed ci g arette, 0, Ol th e separat e ru n , n i coti n e of th e fi l terl ess separat e in mi l l i g rams mai n st ream ci g arettes, val u es for each of s mo ke to th e set of ru n s; of f) al kal oi d th e reten ti on n earest smoki n g d at e mi l l i g rams to ru n s; g) if in mai n st ream ru n s; n earest percen t ag e, for th e the separat e l f”i , , n earest d en sat e separat e ru n s; val u es of ci g arette, d en sat e s moki n g f) expressed ru n , al kal oi d n earest avai l - mi l l i g rams mai n st ream smoki n g 0, Ol d en sat e separat e if ff, i , , ci g arette, n earest smoki n g ru n s; mai n st ream expressed ci g arette, resu l ts; d en sat e vi d u al al kal oi d test if abl e; e) compl et e vi du al separ- smoki n g expressed th e ci g arette, d en sat e, for Fn i c, fi l ter, n i coti n e val u es b) c) al kal oi d ate the of avai l abl e; c) a) met h od : resu l ts; fi l ter, for di rect %; i n d ex Z&, separat e as val u es a percen tag e, for each set to of ru n s; test; to th i s I n tern ati on al g) d at e of test; h) referen ce St an d ard to th i s I n tern ati on al St an d ard I S0 UDC 40 : 9 ( E) 6 76 43 42 47 De scri pto rs: spe ctro me trl c Pri ce based tobacco , meth od on pag es ci g arettes, ci g arette fi l ters, sm o ke , al kal oi d s, tests, ch e m i cal tests, d etermi n ati on , reten tton i n d ex,