nghiên cứu làm chủ công nghệ và xây dựng mô hình công nghiệp sinh học sản xuất giống và rừng trồng cây nguyên liệu giấy năng suất cao

BO CONG THLTONG TONG CONG TY GIAY VIET NAM Vien nghien ciru cay nguyen lieu giay BAO CAO KET QUA DE TAI CAP BO NAM 2009 De tai: NGHIEN CUtJ LAM CHU CONG NGHE VA XAY DUlVG MO HINH CONG NGHIEP SINH HOC • • • SAN XUAT GIONG VA R I T N G TRONG CAY NGUYEN LIEU GIAY NANG SUAT CAO Cc quan chu quan: BO CONG THlTONG Co^ quan chu tri: VIEN NC CAY NL GIAY Chu nhiem de tai: ThS Pham Thi Kim Thanh PHII THO, THANG 12 NAM 2009 -,7/ j JJQA Q BO CONG THl/ONG TONG CONG TY GIAY VIET NAM Vien nghien ciru cay nguyen lieu giay B A O C A O KET QUA DE TAI CAP BO NAM 2009 Be tai: NGHIEN ClTU LAM CHU CONG NGHE VA XAY DUNG MO HINH CONG NGHIEP SINH HOC • • SAN XUAT GIONG VA NGUYEN LIEU GIAY TRONG CAY RITNG NANG SUAT CAO Chii nhiem de tai: ThS Pham Thi Kim Thanh • • • Tham gia thyc hien: KS Vu Thj Lan KS Nguyen Thi Thin KS Trieu Hoang Son PHU THO, THANG 12 NAM 2009 • MUC LUC Thit tw Noi dung Trang Danh muc ky hieu va cac chir viet tat ii Danh muc bang va hinh iii Tdm tit iv I Tdng quan 1.1 Xuat XII cua de tai 1.2 Tinh cap thiet va muc tieu nghien ciin cila de tai 1.3 Ddi tugng va ndi dung nghien cuu 1.4 Tdng quan tinh hinh nghien ciin va ngoai nude 1.4.1 Tinh hinh ling dung ky thuat nuoi cay md va giam hom Bach dan san xuat lam nghiep 1.4.2 Tinh hinh nghien cuu nude 1.4.3 Tinh hinh nghien cuu d nude ngoai 1.4.4 Co sdly thuylt 10 II Thuc nghiem 13 2.1 Phuong phap nghien cuu 13 2.2 Thiet bi, dung cu, nguyen vat lieu va hoa chat su dung cho 17 nghien ciin 2.3 Ket qua thuc nghiem va thao luan 18 2.3.1 Danh gia kha nang nhan gidng in vitro cua ddng Bach dan 18 nghien ciin 2.3.2 Danh gia kha nang re in vitro cua ddng Bach dan nghien 20 cuu 2.3.3 Danh gia kha nang nhan gidng bang hom ciia cac ddng Bach 21 dan nghien cuu 2.3.4 LTng dung cdng nghe nudi cay md va giam hom san xuat 24 cay gidng Bach dan 2.3.5 Xay dung va cham sde vudn vat lieu gidng cac ddng Bach dan 29 2.3.6 Kha nang sinh trudng tai cac md hinh rung 2.3.6.1 Kit qua sinh trudng cua rung trdng tai Lap Thach - VTnh Phiic 2.3.6.2 Kit qua sinh trudng cua rung trdng tai Tam Thanh - Phii Thg III Kit luan va kien nghj Tai lieu tham khao Phan phu luc 30 30 33 36 38 DANH MUC KY HIEU VA CAC CHlT VIET TAT ABT, Che pham re cua Trung Qudc (ABT sd 1) BAP - benzyl adenin purin Dg Dudng kinh gdc Hvn Chieu cao viit nggn lAA Indol axetic axit IBA Indol butyric axit Iv Chi sd sinh trudng hoac chi sd the tich (Iv Dg^*Hvn) MS Mdi trudng Murashige and Skoog (1962) TB Trung binh TN Thi nghiem TT Thiitu WD% He sd bien ddng cua dudng kinh gdc WH% He sd bien ddng cila chieu cao 11 DANH MUC BANG VA HINH Bang 01 Kha nang sinh trudng phat trien chdi Bach dan in vitro 18 ciia cac ddng nghien ciin (sau tuan nudi cay) Bang 02 Chat lugng chdi Bach dan in vitro cua cac ddng nghien 19 cuu Bang 03 Kha nang re in vitro cila chdi Bach dan (sau tuan) 20 Bang 04 Anh hudng cua thdi vu den kha nang re ciia hom ddng 22 Bach dan (lin 1- thang 3/2009 din lin 10- thang 12/2009) Bang 05 Chat lugng cay hom cila ddng Bach dan 23 Bang 06 25 Sinh trudng cua rung trdng 04 ddng vd tinh Bach dan tai Lap Thach - VTnh Phiic (5/2009 - 11/2009 Bang 07 Chat lugng rung trdng 04 ddng vd tinh Bach dan 27 tai Lap Thach - VTnh Phiic (5/2009 - 11/2009) Bang 08 Sinh trudng cila rung trdng 04 ddng vd tinh Bach dan 28 tai Tam Thanh - Phu Thg (6/2009 - 11/2009) Bang 09 Chat lugng rung trdng cua 04 ddng vd tinh Bach dan 30 tai Tam Thanh - Phil Thg (6/2009 - 11/2009) Hinh 01 Ty le re in vitro cua cac ddng Bach dan 21 Hinh 02 Ty le re cua hom cac ddng Bach dan cac thang 22 Hinh 03 Sinh trudng Dg cua rimg trdng tai Lap Thach 26 Hinh 04 Sinh trudng Hvn cua rung trdng tai Lap Thach 27 Hinh 05 Sinh trudng Dg cila rimg trdng tai Tam Thanh 29 Hinh 06 Sinh trudng Hvn cua rimg trdng tai Tam Thanh 29 111 r Tom tat Di nhdn nhanh nhirng giong Bach dan uv viet cho sdn xuat, gdp phdn ndng cao ndng sudt rung, ndm 2009 Bd Cdng Thuong dd giao cho Vien nghien ciru cay nguyen lieu gidy (FRC) nghien ciru di tdi "Nghien ciru lam chii cong nghe vd xdy dung mo hinh cong nghiep sinh hgc sdn xuat gidng va rwng cay nguyen lieu giay ndng suat cao" Ndm 2007 - 2008 de tdi dd hoan thdnh cdc ndi dung sau: Dd xdc dinh vd lira chgn ddng Bgch ddn uu trdi, ndng sudt cao da dugc cdng nhdn gidng (giai dogn 2000 - 2006); Nghien ciru nudi cay md vd gidm hom cdc ddng vd tinh Bgch dan da dugc cdng nhdn gidng: PN3d; PN116; PN54;PN46 Ndm 2009, sau 12 thdng thuc hien vdi su cdng tdc ciia Phdng nghien ciru nudi cay md, Phdng Lam sinh - xd hdi- mdi trudng, de tdi da hoan thdnh nhirng ndi dung nghiin ciru dgt ndm 2009 cu the nhu sau: Dd ddnh gid khd ndng nhdn gidng in vitro cua 04 ddng Bgch dan nghien ciru vi khd ndng nhdn nhanh vd sinh trudng cua chdi in vitro, khd ndng ri cua chdi in vitro; Ddnh gid khd ndng nhdn gidng bdng hom cua 03 ddng Bgch dan nghien cuu; Xdy dung vudn vat lieu gidng Bgch ddn vd 02 md hinh rimg trdng Bgch ddn; Ung dung ky thuat nudi cay md — gidm hom Bgch dan theo hudng cdng nghiep: tgo 20.000 cdy gidng IV Phan I TONG QUAN 1.1 Xuat xu cua de tai Bach dan la loai cay cd kha nang sinh trudng nhanh, cd bien sinh thai rdng, de gay trdng tren nhieu dang lap dia, nang suat cao, chu ky kinh doanh ngan, cd kha nang chiu gid bao tdt Dac biet ddi vdi Bach dan Urophylla, la cay cd dang than thang, thon dep, tia canh tu nhien tdt nen thu hiit nhieu ngudi su dung Hien Bach dan cd dien tich trdng rimg kha Idn d nude ta, la cay quan trgng de san xuat bdt giay Tir nam 1979 Trung tam nghien cuu cay nguyen lieu giay Phu Ninh (nay la Vien nghien cuu cay nguyen lieu giay) da bat dau cac khao nghiem chgn gidng bach dan de phuc vu cho viec trdng rung tham canh cay nguyen lieu giay Cac ket qua nghien ciin cua Trung tam da xac dinh dugc xuat xu bach dan E.urophylla tu Lewotobi va Egon ludn dung dau ve sinh trudng d tat ca cac khao nghiem Do dd loai va xuat xu da dugc nhap hat gidng va gay trdng rung md rdng d vung trung tam Bac Bd Tir dien tich riing trdng dd Trung tam Phu Ninh da chgn tuyen dugc hang tram cay trdi dua vao khao nghiem tuyen chgn cac ddng uu trdi cho san xuat Nhieu thi nghiem ddng vd tinh cho loai bach dan E.urophylla dugc thiet lap tu nam 1997 den nam 2000 Kit qua din nam 2006 cac ddng PN2 ; PN14; PN3d; PN46; PN47; PNl 16 da dugc bd Ndng nghiep va phat trien ndng thdn cdng nhan la gidng dua trdng rimg cdng nghiep d viing Trung tam va cac viing cd dieu kien sinh thai tuong tu Nhiing ddng Bach dan dugc cdng nhan gidng PNl 16; PN3d; PN46 cd nang suat cao, song hien chua cd nghien cuu cu the ve ky thuat nhan gidng va lam ehu cdng nghe san xuat gidng cho timg ddng D I gdp phan nang cao nang suat rung nguyen lieu giay, nam 2008 Bd Cdng Thuong giao cho Vien nghien cuu cay nguyen lieu giay (FRC) nghien Clin de tai "Nghien ciiu lam chii cong nghe vd xdy dwng mo hinh cong nghiep sinh hpc sdn xuat gidng va rwng cdy nguyen lieu gidy ndng sudt cao" DO ban che ve kinh phi, Hdi ddng khoa hgc ciia Vien va Vu khoa hgc cdng nghe cua Bd nhit tri gidi ban dk tai tap trung nghien cuu vao mot loai Bach dan E.urophylla vdi cac ddng PN3d, PN46, PN54, PNl 16 va ke hoach dl tai thuc hien nam (2007 - 2009) 1.2 Tinh cap thiet va muc tieu nghien cuu ciia de tai 1.2.1 Tinh cap thiet ciia de tai Ddng thdi vdi viec chgn cay trdi la viec nghien ciin ung dung ky thuat nhan gidng vd tinh bang giam hom va nudi cay md, tao kha nang dua nhanh mot sd ddng bach dan cao san (PN2; PNl4) vao thi nghiem va san xuat Hang ngan rung md-hom vdi cac ddng PNl4; PN2 dugc trdng d tinh Phu Thg, Tuyen Quang, Yen Bai, VTnh Phiic, Bac Giang, Lang Son, Thai Nguyen, Quang Ninh, Nghe An, Ha TTnh, Phii Yen Tuy nhien sd ddng vd tinh bach dan dua trdng cdn it, nen d mot vai noi da cd dau hieu nguy co sau benh hai Viec nghien ciin ky thuat nhan gidng va lam chu cdng nghe san xuat gidng nhimg ddng mdi can dugc tien hanh de cung cap cay gidng cho san xuat, phuc vu cac chuong trinh trdng rung cho loai Bach dan Vi vay de tai "Nghien ciru lam chu cdng nghi vd xdy dung md hinh cdng nghiep sinh hgc sdn xuat gidng vd rimg trdng cdy nguyen lieu gidy ndng sudt cao " dugc thuc hien 1.2.2 Muc tieu nghien cuu ciia de tai * Muc tieu tdng quat: + Lam chu cdng nghe nhan gidng md-hom va ung dung nhan giong mot sd ddng vd tinh Bach dan uu trdi, nang suat cao da dugc cdng nhan gidng (giai doan 2000 - 2006): PNl 16; PN3d; PN54; PN46 + San xuat cay gidng vd tinh Bach dan * Muc tieu cu the ndm 2009: + Xac dinh kha nang nhan gidng bang ky thuat nudi cay md va giam hom cila cac ddng Bach dan nghien ciin + Xay dung md binh rimg trdng Bach dan 1.3 Doi tugng/pham vi va noi dung nghien cuu 1.3.1 Doi tuong nghien cuu: Cac ddng vd tinh Bach dan urophylla da dugc cdng nhan gidng cip qudc gia: PN46; PN54; PNl 16; PN3d (giai doan 2000 - 2006) dugc dan gidng tu rung trdng thi nghiem cua Vien nghien cuu cay nguyen lieu giay Phu Ninh - Phii Thg + PN3d: La ddng da dugc Bd Ndng nghiep va Phat trien ndng thdn cdng nhan gidng Qudc gia nam 2003 + PNl 16 : La ddng da dugc Bd Ndng nghiep va Phat trien ndng thdn cdng nhan gidng tien bd ky thuat nam 2006 -I- PN54: La ddng da dugc Bd Ndng nghiep va Phat trien ndng thdn cdng nhan gidng tien bd ky thuat nam 2005 + PN46 : La ddng da dugc Bd Ndng nghiep va Phat trien ndng thdn cdng nhan gidng tien bd ky thuat nam 2004 1.3.2 Noi dung nghien cuu San xuat giong (san xuat thu ddng cd kha nang nhan nhanh): 20.000 cay md Bach dan * Nudi cay md : he sd nhan gidng > lan ; ty le re > 85% * Hom giam cd ty le re tir 70 - 80%, cay hom khoe manh, sinh trudng tdt dat ty le xuat vudn > 85% - Danh gia kha nang nhan gidng in vitro ciia ddng Bach dan nghien cuu + Kha nang nhan nhanh va sinh trudng cua chdi in vitro + Kha nang re cua chdi in vitro - Danh gia kha nang nhan gidng bang hom cua ddng Bach dan nghien cuu: + Kha nang re ciia hom giam + Ty le xuat vudn va chat lugng cay Xay dung vudn vat lieu gidng Bach dan Xay dung md hinh rung trdng Bach dan 1.4 Tong quan tinh hinh nghien ciru va ngoai nude, co sd ly thuyet 1.4.1 Tinh hinh ung dung ky thuat nudi cay md va giam hom Bach dan san xuat lam nghiep Hien nhieu nude phat trien da thuc hien kinh doanh rung nguyen lieu theo phuong thuc dau tu tham canh cao De cd cay gidng chat lugng tdt phuc vu trdng riing, cac nude tap trung vao viec san suat cay theo phuong phap nhan gidng md - hom, cdng nghe dugc nhieu noi tren the gidi ap dung de nhan nhanh nhung ca the chgn Igc tao cay chat lugng cao dap ling dugc nhu cau trdng rung d qui md Idn Nhimg rimg trdng bang cay tir md-hom da dat dugc ddng deu rat cao, tri dugc nhirng dac tinh uu trdi cila cay me (sinh trudng nhanh, nang suat va chat lugng cao) Song song vdi viec chgn Igc nhimg ddng va xuat xu tdt, viec nhan gidng nhiing ddng dugc chgn tuyen va lam chu cdng nghe nhan gidng nhiing ddng dd la van de quan trgng, vay cac nude nhu Cdng hoa Nam Phi, Brazin, Trung Qudc da nghien cuu va xay dung nhiing vudn uom san suat hang chuc trieu cay md-hom/nam va toan bd cdng viec dugc thuc hien tren mot day chuyen cdng nghiep Trung Qudc la nude ung dung sdm va cdng nudi cay md vao trdng rimg dien rdng, cay dugc nhan gidng cdng chinh la E urophylla Den nam 1991 d vung Nam Trung Qudc, ngudi ta da tao tren trieu cay md cua cac cay va ddng lai dugc chgn Igc [5] Sd cac loai bach dan da dugc nhan gidng bang phuong phap nudi cay md mot tang, tdi nam 1987 da cd tren 20 loai Bach dan khac tao dugc cay in vitro (Gupta va Mascarenhas, 1987) Cac nha khoa hgc An Do da tao cdng cay md tir cac cay trdi bach dan E.camandulensis, E.globulus, E tereticornis, E torelliana va ca tu cac cay trdi cd ham lugng tinh dau cao cila bach dan chanh E citriodora Cay md cd ngudn gdc tir cay uu viet sinh trudng nhanh gap lan va ddng dlu hon la cay mgc tu hat cua cung cay me Tai Uc, nhan gidng bang nudi ciy in vitro da dugc ap dung dk so DO virons VAT LIEU BACH DAN PN3d: 8h (270 cay) PN116:9h (365 cay) Thap nu-ffc PN54: 2h (40 cay) PN46: 2h (60 cay) PNl 16: 17h (475 cay) Bach dan - dan dong PN3d: 9h (290 cay) Vudn vat lieu gidi Ghi chii: h = hang hang = 34 cay Van phdng Bd phan ling dung Phu luc 1: BIEU SO LIEU KHI TI/ONG THUY V A N THANG Nhiet Nhiet KK TB thang Nhiet KK toi thap TB thang NAM 2009 Cf VUNG VIET BAC (TRAM Luong mua So Tongluong C mua O mua thang (mm) thang (ngay) VIET TRI) Do am Boc h d Nang Do am KKTB thang (%) Tong luong bdc hoi thang (mm) Tdng so gid nang thang (gid) 77 53 113 CC) Nhiet KK toi cao TB thang ("O 12/2008 17,7 21,9 14,9 01/2009 15,6 19,7 12,8 22 78 61 103 02/2009 22,0 25,6 19,8 21 83 50 76 3/2009 20,7 23,9 18,6 31 14 84 48 54 4/2009 24,5 28 21,8 125 15 82 60 109 5/2009 26,8 30,7 24,1 122 18 81 74 137 6/2009 29,7 34 26,2 278 12 78 91 180 7/2009 28,9 32,7 26,4 239 16 83 69 147 8/2009 29,4 34,5 27,0 154 15 80 82 200 9/2009 28,9 33,5 25,7 92 11 76 89 167 10/2009 26,2 30,1 23,5 21 79 75 73 11/2009 Trung binh nam 21,3 25,4 18,5 72 87 132 24,3 28,3 21,6 105,8 10,9 79,8 66,8 125,3 Thang ro Phu luc 2: Thanh phan NH4NO3 KNO3 KH2PO4 CaCl2.4H20 Ca(N03)2 MgSO47H20 NaH2P04 KCl MnS04.7H20 ZnS04.7H20 H3BO3 KI CUSO4.5H2O Na2Mo04.2H20 C0CI2.6H2O FeS04 7H2O NaEDTA Inositol Thiamine HCl Nicotinic acid Pyridoxine HCl Acid ascorbic Glycine CAC MOI T R U O N G NUOI C A Y D A S U DUNG MS Ml M3 Cac chat da lu-o-ng (mg/I) MS2 1650,0 1000,0 750,0 1237,5 562,5 937,5 1900,0 1900,0 1425,0 1425,0 1068,8 1781,3 170,0 170,0 127,5 170,0 95,6 159,4 440,0 440,0 330,0 550,0 247,5 412,5 370,0 370,0 277,5 462,5 208,1 346,9 370,0 370,0 277,5 462,5 208,1 346,9 170,0 0,0 0,0 170,0 0,0 170,0 90,0 90,0 67,5 Cac chat vi lu-ong (mg/1) 112,5 50,6 84,4 MSI MS3 22,3 22,3 22,3 22,3 22,3 22,3 8,6 8,6 8,6 10 10 10 6,2 6,2 6,2 10 10 10 0,83 0,83 0,83 0,83 0,83 0,83 0,025 0,025 0,025 0,025 0,025 0,025 0,25 0,25 0,25 0,25 0,25 0,25 0,025 0,025 0,025 0,025 0,025 0,025 37,3 37,3 37,3 37,3 37,3 37,3 27,8 27,8 27,8 Vitamin (mg/1) 27,8 27,8 27,8 100 100 100 100 100 100 1 1 5 0,5 5 0,5 0,5 0,5 0,5 0,5 0,5 10 10 10 Cac chat hu-u co- (mg/1) 10 10 10 2 BAP 1 lAA 0,4 0,5 2 C lat dieu hoa sinh tru-6-ng (mg/1) Phu luc 3: KET QUA PHAN TICH XAC DINH KHA NANG NHAN GIONG BANG NUOI CAY M 04 D6NG BACH DAN ANOVA Chieu cao TB cua choi Between Groups Sum of Squares 140,263 df Mean Square 46,754 1,356 Within Groups So cap la 756,513 558 Total 896,776 F 34,486 Sig ,000 31,450 ,000 561 Between Groups 130,182 43,394 Within Groups 769,917 558 1,380 Total 900,100 561 Chieu cao TB cua choi So cap la Duncan' Duncan Dong vo tinh PN46 DongPN116 Dong PN54 PN3d Sig N 72 202 136 152 Subset for alpha = 05 1,4083 2,4921 2,5353 3,1000 ,772 1,000 1,000 Dong vo tinh PN46 PN3d Dong PNl16 Dong PN54 Sig Means for groups in homogeneous subsets are displayed N 72 152 202 136 Subset for alpha = 05 = 2,5278 3,3816 3,5644 4,1618 1,000 ,225 1,000 Means for groups in homogeneous subsets are displayed, a Uses Harmonic Mean Sample Size = 122,058 a- Uses Harmonic Mean Sample Size = 122,058 b The group sizes are unequal The harmonic mean of the group sizes is used Type I error levels are not guaranteed b The group sizes are unequal The harmonic mean of the group sizes is used Type I error levels are not guaranteed Loai choi * Dong vo tinh Crosstabulation Dong vo tinh Dong PN54 Loai Choi Dong PNl 16 Dong PN3d Dong PN46 Total Choi loai (tot) Count o/„ yyjthin Dong vo tinh 57,4% 55,4% 53,9% 5,6% 49,1% Choi loai (TB) Count % within Dong vo tinh 29,4% 13,9% 26,3% 27,8% 22,8% Choi loai (xau) Count % vi^ithin Dong vo tinh 13,2% 30,7% 19,7% 66,7% 28,1% Count % within Dong vo tinh 100,0% 100,0% 100,0% 100,0% 100,0% Total Chi-Square Tests Pearson Chi-Square Value 96,419^ Asymp Sig (2-sided) ,000 Likelihood Ratio 106,926 ,000 Linear-by-Linear Association 43,404 ,000 N of Valid Cases df 562 a- cells (,0%) have expected count less than The minimum expected count is 16,40 Phu luc 4: KET QUA PHAN TICH K H A NANG RA RE CUA cAc DONG BACH DAN Kha nang re * Dong vo tinh Crosstabulation Kha nang re Ra re Khong re Total Count % within Dong vo tinh Count % within Dong vo tinh Count % within Dong vo tinh Dong PN116 543 67,0% 267 33,0% 810 100,0% Dong vo tinh Dong PN54 Dong PNSd 480 546 59,3% 67,4% 330 264 40,7% 32,6% 810 810 100,0% 100,0% Dong PN46 78 16,0% 408 84,0% 486 100,0% Total 1647 56,5% 1269 43,5% 2916 100,0% Chi-Square Tests Value 401,826^ 420,604 Pearson Chi-Square Likelihood Ratio Linear-by-Linear Association N of Valid Cases 3 Asymp Sig (2-sided) ,000 ,000 ,000 df 190,922 2916 a cells (,0%) have expected count less than The minimum expected count is 211,50 Kha nang re * IVIoi truong re Crosstabulation Kha nang re Ra re Count % within Moi truong re Khong re Count % within Moi truong re Count % within Moi truong re Total Moi truong MR-1 507 52,2% 465 47,8% 972 100,0% Chi-Square Tests Pearson Chi-Square Likelihood Ratio Linear-by-Linear Association N of Valid Cases 12,266 2 Asymp Sig (2-sided) ,002 ,002 11,768 ,001 Value 12,280^ df 2916 a cells (,0%) have expected count less than The minimum expected count is 423,00 Moi truonq re Moi truong Moi truong MR-2 MR-3 558 582 57,4% 59,9% 414 390 42,6% 40,1% 972 972 100,0% 100,0% Total 1647 56,5% 1269 43,5% 2916 100,0% Phu l c KET QUA PHAN TICH K H A N A N G N H A N GIONG BANG HOM u : CUA CAC DONG BACH DAN Tests of Between-Subjects Effects Dependent Variable: So hom re Source Corrected Model Intercept DVT LTN Error Total Corrected Total Type III Sum of Squares 673099,937^ 7212268,488 432671,118 197385,661 331020,940 10032721,0 1004120,878 df Mean Square 61190,903 7212268,488 216335,559 21931,740 180,787 11 1831 1843 1842 F 338,470 39893,741 1196,632 121,313 Sig ,000 ,000 ,000 ,000 a- R Squared = ,670 (Adjusted R Squared = ,668) So hom 13 re Duncan Subset Dong vo tinh Dong PN46 N 452 Dong PN3d 569 Dong P N l 16 48,4248 822 Sig 62,6907 86,9051 1,000 1,000 1,000 Means for groups in homogeneous subsets are displayed Based on Type III Sum of Squares The error tenm is Mean Square(Error) = 180,787 a Uses Harmonic Mean Sample Size = 578,436 b The group sizes are unequal The harmonic mean of the group sizes is used Type I error levels are not guaranteed c Alpha = ,05 So hom re Duncan Subset Lan thu nqhiem Lan N 146 49,0274 Lan 166 56,8554 Lan 10 163 56,8896 Lane 173 Lan 176 67,2386 Lan 185 69,3351 62,5491 Lan 199 78,3166 Lan 195 79,8000 Lan1 208 80,5865 Lan 232 Sig 85,0690 1,000 ,981 1.000 ,138 Means for groups in homogeneous subsets are displayed Based on Type III Sum of Squares The error term is Mean Square(Error) = 180,787 a Uses Harmonic Mean Sample Size = 181,332 b The group sizes are unequal The harmonic mean of the group sizes is used Type I error levels are not guaranteed c Alpha = ,05 ,130 1,000 Ranks Chi so re Dong Dong Dong Dong Total vo tinh votinh PNl 16 vo tinh PN3d vo tinh PN46 N Mean Rank 627,18 841,36 882,96 900 183 350 1433 Test Statistics?" Chi-Square df Asymp Sig Chi so re 115,236 ,000 a Kruskal Wallis Test b- Grouping Variable; Dong vo tinh ANOVA Chi so rare PNl 16 Between Groups Within Groups Total Sum of Squares 646,698 113590,1 114236,8 df 890 899 F Mean Square 71,855 127,629 ,563 Chi so re PNSd Duncan a,b Lan thi nghiem Lan lan Lan Lan Lan 10 lan lan Lan1 Lan Lan Sig N 23 49 59 64 35 23 49 32 = Subset for alpha = 22,1609 28,7204 29,7390 30,6844 31,1200 32,5609 32,8959 34,9844 05 32,5609 32,8959 34,9844 38,3863 51 40 38,9725 1,000 ,100 ,081 Means for groups in homogeneous subsets are displayed a Uses Harmonic Mean Sample Size = 37,768 b- The group sizes are unequal The harmonic mean of the group sizes is used Type I error levels are not guaranteed Sig ,828 Test Statistics?'' Chi-Square df Asymp Sig Chi so re PN46 61,391 ,000 a Kruskal Wallis Test b- Grouping Variable: Lan Phu luc 6: KET QUA P H A N TICH K H A NANG SINH T R U O N G CUA CAC DONG BACH DAN TAI LAP THACH ANOVA Chieu cao vut ngon (m) Dng kinh goc (cm) Sum of Squares 92,149 71,939 164,088 92,298 167,374 259,672 Between Groups Within Groups Total Between Groups Within Groups Total df 391 394 391 394 Mean Square 30,716 ,184 F 166,948 Sig ,000 30,766 ,428 71,872 ,000 Chieu cao vut ngon (m) Dong vo tinh Bach dan Duncans': Dong PN54 Dong PN3d Dong PN14 Dong PNl16 Sig 1,9624 N 93 103 94 105 Subset for alpha = 05 2,1243 2,2787 1,000 1,000 1,000 3,1876 1,000 Means for groups in homogeneous subsets are displayed a Uses Harmonic Mean Sample Size = 98,465 b The group sizes are unequal The harmonic mean of the group sizes is used Type I error levels are not guaranteed Dng kinh goc (cm) Dong Duncans': Dong Dong Dong vo tinh Bach dan PN3d PN54 PNl4 DongPN116 Sig N 103 93 94 105 Subset for alpha = 05 2,8129 2,8369 3,7051 3,8565 ,797 Means for groups in homogeneous subsets are displayed a Uses Harmonic Mean Sample Size = 98,465 b- The group sizes are unequal The harmonic mean of the group sizes is used Type I error levels are not guaranteed ,105 Phu luc 7: KET QUA P H A N TICH K H A N A N G SINH TRUONG CUA cAC DONG BACH D A N TAI TAM THANH ANOVA Chieu cao vut ngon Duong kinh goc Between Groups Within Groups Total Between Groups Within Groups Total Sum of Squares 35,797 64,677 100,474 14,662 173,105 187,768 df 369 372 369 372 Mean Square 11,932 ,175 F 68,077 Sig ,000 4,887 ,469 10,418 ,000 Chieu cao vut ngon Duncan Dong Dong Dong Dong Dong Sig a.b Bach dan PNl4 PN54 PN3d PNl16 N 79 84 103 107 1,5810 Subset for alpha = 05 1,8595 2,2028 1,000 1,000 1,000 2,3981 1,000 Means for groups in homogeneous subsets are displayed a Uses Harmonic Mean Sample Size = 91,706 b The group sizes are unequal The harmonic mean of the group sizes is used Type I error levels are not guaranteed Duong kinh goc Duncan Dong Dong Dong Dong Dong Sig a,b Bach dan PN54 PN3d PNl4 PNl16 N 84 103 79 107 Subset for alpha = 05 2,7543 2,8550 2,9523 3,2659 1,000 ,064 Means for groups in homogeneous subsets are displayed a Uses Harmonic Mean Sample Size = 91,706 b The group sizes are unequal The harmonic mean of the group sizes is used Type I error levels are not guaranteed PHU LUC: So lieu dem choi re, sinh truong ciia re cay in vitro PNl 16 Moi tryong MR-1 PN54 PNSd PN46 MR-2 MR-3 MR-1 MR-2 MR-3 MR-1 MR-2 MR-3 MR-1 MR-2 MR-3 So binh cay/Ian (binh) 5 5 5 5 3 So choi cay/Ian (choi) 90 90 90 90 90 90 90 90 90 54 54 54 48 63 78 36 48 60 72 60 45 9 53,3 70,0 86,7 40,0 53,3 66,7 80,0 66,7 50,0 16,7 11,1 16,7 45 60 75 45 51 69 75 66 42 12 6 50,0 66,7 83,3 50,0 56,7 76,7 83,3 73,3 46,7 22,2 11,1 11,1 36 60 78 45 60 66 72 69 45 12 9 Ty le rl (%) 40,0 66,7 86,7 50,0 66,7 73,3 80,0 76,7 50,0 22,2 16,7 16,7 Ty le re TB(%) 47,8 67,8 85,6 46,7 58,9 72,2 81,1 72,2 48,9 20,4 13,0 14,8 Lan Tong so choi re (choi) Ty le re (%) Lan Tong so choi re (choi) Ty le re (%) Lan Tong so choi re (choi) MOT SO HINH ANH KET QUA THi NGHIEM TRONG QUA TRINH NGHIEN ClTU Anh Yuan vat lieu dong Bach dan: PNl 16; PN3d; PN54; PN46 Anh 2: Cay cap hom Bach dan PN3d Anh 3: Cay cap hom Bach dan PN116 Anh 4: Cay cap hom Bach dan PN54 Anh 5: Cay cap hom Bach dan PN46 Anh 6: Sinh truang va phat trien choi PNl 16 tren moi truang MS2 bo sung 0.8 mg/1 BAP va 0.4 mg/1 lAA (sau 25 nuoi cay) iM ^ f i' '^^ ^H inr n, • , .3F i Anh 7: Sinh truang va phat trien choi PNSd tren moi truang MS2 bo sung 1.0 mg/1 BAP va 0.2 mg/1 lAA (sau 25 nuoi cay) r ti^^^.,., - ^i^a^mm^ ^• Anh 8: Sinh truang va phat trien choi PN3d tren moi truang MS2 bo sung 1.0 mg/1 BAP va 0.4 mg/1 lAA (sau 25 nuoi cay) MQt so hinh anh rung thi nghiem Bach d^n thang tuoi Tai DQI Th^nh Cong - CTLN Lap Thach - 2009 '^mn PN14 PN3d 'I'' \\, < PN116 PN54 ... tieu danh gia la: + Sinh trudng chieu cao: chieu cao viit nggn Hvn (m) + Sinh trudng dudng kinh: dudng kinh gdc Dg (cm) + Cap sinh trudng va theo ddi tinh hinh sau benh hai Chieu cao viit nggn (Hvn):... 3,19 m Ddng cd sinh trudng ve chieu cao kha nhat la PNl 16, ddng cd sinh trudng ve chieu cao kem nhat la PN54 31 — ^ g, ^«„ wctj^ oiiih trudng thay ty le cay sinh trudng cap rat cao, trung binh... lieu giii d cao khdng qua 40 cm sau cat tao chdi Cd the cat cao hon neu nhu d cao it chdi Cay me ludn tri d cao tir 30 - 40 cm de tang tinh tre hoa ciia than va kha nang bat chdi mdi Do cao ciia