[...]... Cornish-Bowden, A (1976) Principles of enzyme kinetics Butterworth, London Cornish-Bowden, A & Wharton, C W (1988) Enzyme kinetics IRL Press, Oxford Cornish-Bowden, A (1995) Fundamentals of enzyme kinetics Portland Press, London Dixon, M & Webb, E.C (1979) Enzymes Academic Press, New York Edsall, J T & Gutfreund, H (1983) Biothermodynamics J Wiley & Sons, New York Eisenthal, R & Danson, J M (1992) Enzyme assays... Press, Oxford Engel, P C (1977) Enzyme kinetics Chapman & Hall, London Fersht, A (1977) Enzyme structure and mechanism Freeman & Co., San Francisco Fromm, H J (1975) Initial rate kinetics Springer-Verlag, Berlin Klotz, I M (1986) Introduction to biomolecular energetics including ligand-receptor interactions Academic Press, Orlando Kuby, S A (1991) Enzyme catalysis, kinetics and substrate binding CRC Press,... Part B: Methods in Enzymology, Vol 64 (1980); Part C: Methods in Enzymology, Vol 87 (1982); Part D: Methods in Enzymology, Vol 249 (1995); Part E: Methods in Enzymology, Vol 308 (1999) Academic Press, New York Purich, D L (1996) Contemporary enzyme kinetics and mechanism Academic Press, New York Purich, D L (1999) Handbook of biochemical kinetics Academic Press, New York Piszkiewicz, D (1977) Kinetics. .. beyond the limits of enzyme kinetics in a narrow sense, dealing in the Greek meaning of the word, (vimgrir – motion), with time-dependent processes A standardised nomenclature throughout the book links the individual chapters For the enzyme in enzyme kinetics, as well as for the non-enzymatic macromolecule in multiple equilibria, “E” is uniformly used Enzyme substrates and ligands in binding processes... Wong, J T.-F (1975) Kinetics of enzyme mechanisms Academic Press, London Nomenclature instruction Nomenclature Committee of the International Union of Biochemistry (NC-IUB) (1982) Symbolism and terminology in enzyme kinetics European Journal of Biochemistry 128, 281–291 International Union of Pure and Applied Chemistry (1981) Symbolism and terminology in chemical kinetics Pure and Applied Chemistry... whereas its substrate, lactic acid (Mr = 90.1), would cover Enzyme Kinetics: Principles and Methods Hans Bisswanger Copyright © 2002 WILEY-VCH Verlag GmbH, Weinheim ISBN: 3-527-30343-X 6 1 Multiple Equilibria 170 m at the same time, and a water molecule (Mr = 18) would even cover 370 m Enzyme and substrate would pass each other like rifle balls and would arrive at the cell wall within fractions of a second... (1973) The chemical kinetics of enzyme action, 2 edn Clarendon Press, Oxford Lasch, J (1987) Enzymkinetik Springer-Verlag, Berlin Lüthje, J (1990) Enzymkinetik Urban & Schwarzenberg, München Plowman, K M (1972) Enzyme kinetics McGraw-Hill, New York Price, N C & Stevens, L (1989) Fundamentals of enzymology Oxford University Press, Oxford Purich, D L Enzyme kinetics and mechanics Part A: Methods in Enzymology,... dissociation constants) are obtained, and interactions between enzyme and ligands can be analysed in the absence of a catalytic turnover This procedure simplifies the treatment of complex mechanisms, e.g., of allosteric enzymes Mechanisms that can entirely be characterized by multiple equilibria will be described in this chapter, although they often are analysed by enzyme kinetic methods, since enzymatic turnover... constant of the forward reaction Enzyme Kinetics: Principles and Methods Hans Bisswanger Copyright © 2002 WILEY-VCH Verlag GmbH, Weinheim ISBN: 3-527-30343-X XIV k–1 kcat kB kat Mr m n nh NA Or P, Q, R P R r q DS Sl T t H s U v v0 V Y Symbols and Abbreviations rate constant of the reverse reaction catalytic constant Boltzmann constant (kB = R/N = 1.38·10–23 J·K–1) Katal, enzyme unit according to the SI... Piszkiewicz, D (1977) Kinetics of chemical and enzyme- catalyzed reactions Oxford University Press, Oxford Roberts, D V (1977) Enzyme kinetics Cambridge University Press, Cambridge Schellenberger, A (1989) Enzym-Katalyse Springer-Verlag, Berlin Schulz, G E & Schirmer, R H (1979) Principles of protein structure Springer-Verlag, Berlin Segel, I H (1975) Enzyme kinetics John Wiley & Sons, New York Suelter, . Bisswanger Enzyme Kinetics Principles and Methods Enzyme Kinetics: Principles and Methods. Hans Bisswanger Copyright © 2002 WILEY-VCH Verlag GmbH, Weinheim ISBN: 3-527-30343-X Hans Bisswanger Enzyme Kinetics Principles. Contemporary enzyme kinetics and mechanism. Academic Press, New York. Purich, D.L. (1999) Handbook of biochemical kinetics. Academic Press, New York. Piszkiewicz, D. (1977) Kinetics of chemical and enzyme- catalyzed. Order Reactions 54 Enzyme Kinetics: Principles and Methods. Hans Bisswanger Copyright © 2002 WILEY-VCH Verlag GmbH, Weinheim ISBN: 3-527-30343-X 2.2 Steady-State Kinetics and the Michaelis-Menten