Tai lieu tn hoa sinh cong nghiep bai tlc, enzyme, vtmc, chat beo

TA ST C U H M 3"2.1 TA IL IE U TLC) 3.2.x6co-!nh loai tipit beng sac try l6p nning ffhin-I-ayer-cliromatograpkrS" Ly thuyeit vd sic ki !6P m6ng J a l\ ^ g?iyeTLaa.c pha trnh hdp dQng, A day II- chAll6ng, chuydn mflp 'qu-a ving chria P pban {-c-b s-e qu-9-9!a-chring' H 9?y.,_ _\{cen U Tg IL IE U M c5'c chdt duqc phin tach chuydn dich lon hon so v6i chAt c6 6i luc ldn hon- Nhd vay mli b c6c vi td kh6c nfau Trong sec kf I6p n6ng, pha trnh Ii dung mOi qud rrinh cha,v sac k! TA ST C O pha dOng !o tx-ong t-6'-p:r.(f.-c!'6t ir6p -t!g-nhd.-tin-Lr-gg chuvdnd6.ng cham hon tro-tg.be thdlg p4q hi Qlat su rudng t6.c niLy mlL chft phan tich 1E nhanh hop vi a@-toi:ffi-oilts sa-i-}, ph-dii tich c6 6i lu_c y6u hon vti ifitrin-rt"tr"vdn ddng rhU U vl ST pha tinh .C O M sic ky trdh gi5y, sac hi lqp Tuong tu nhu mgi ky thuat sdc }cf kh6c, dic biet 1i-v6i cdn ph5'n tfch duoc cho tuong tdc v6i hai gr6ngd-Uq trc-LnQ! ngltye! ticrdtdoll-g!-fu: Chdt IL I EU H qu6 trinh sac ky, c6c plian tLi (duoc dDng dti chudn bi lqD rn6ng h{p thu) Tuy nhien,uong vdi.chdt mart-9 c6 dinh v) trd dung mOi c6 cuc chuytin dOng qua chdt mang c6 thd iidn ket thhnh pha trnh U TA b Chdt nrang itlta tuilt H M m6t bln m6ng cfia cellulose, thuy tinh hiy nhua' drroc phri bin gidy cellulose ding cho sac k! rn6r lop nr6ng silicagel, nh6rn hay cellulose c6 thd ding duoc lim sin v6i ciLc wcb gidy lem pha unh k! thuat TLC Th6ng thudng, cdc ban TLC 1) TA IL ST li3u v)' chitiu diy.kh5-c nhau' U vlt H thu6c, IE C U O chdt mang day c6 thd U c Pha d6ng LI E C6 nhidu ning lua chqn chay sic I ki v) n-rOt h€ dung rnOi mdr- qui g loat c6c hd dung m6i, c6 kdm theo cdc rn5u ehr;iti:'i., + inr Hoat doa vi proteaza IE , ax-it amin cria cdc enzim v U (LrDP) IL )oit C - chu M lhi O TA IL I hodc hemogibbin) 0,lM IE TA -Dung dich.d0m v4n nang IL ST C 5.7.2Hol' chdt vir dung dich enzim M ml a-rit axetic bang nudc cat t6i lit dung dich 0,livl : IL t IE U H U IL IE TA H U ST C O M U ST + Pha dung dich (B) axit photphoric O Hod ran 5,7 C U + Pha aung ai"n (A) axit a-retic O,1N{ TJ TA ST C U F,r: ,: , TA IL IE U H M ' :,1:;r,i axit phophoric = P -'- - l i{) nu6c cft tfi I Ut dung dich' arit octo-boric 0,llvl O C U pii: :.lti3rrvhohSnhqpdOmnlyc6clugrrgNaoHlNkhd.cnhau,taduocciic dur :::r- j:n c6 PH til 1,8 toi 12'0' cic: :::- .zr ring 0,5M v6i ldy L{r' =- - - :::-z ho6 chft dd pha c5c dung dich A' B' C tren gfp IAn so IL IE U H M ST C O -Dung ta duoc h6n hgp d6m c6 ST Hcl :-, '5 NaOH tron! nu6c c{rtor Iit dung dich' Tl,-i:t :_- :1: ,hri tich bang cria cric dung dich A, B, c, M ,{ci:-:.i3axiiocto-borictrongnudccattitillitdungdich' + Fha:::z :ich @) nahi hYdroxit 1N : dd TA EU H U ;r j: -.:l!1 ii:c 1dy 2l,5m:axir bdng; 26,25mlaxit photphoric v)' 30,99 ant boric)' d€rn 0, lM- Dung :::: _3_, - -; :ang O,Oilv[ tr6n thd tich nu6c c{t v6i m6t thd tfch - Dung ci ' i- :-::-xe[ic 0,3M U : _^^:= :.: _ _ :ir toi vach mric- U felin : Chudn bi nhu phAn l'5-2 lit dung dich-.-) :ha icing 16,45m1 Ho dam dac bing il16c c{t't6i' rn6t ::: H :i:- -., -j u,jc ihrr U - Duns ::: - Dung -i:.- ::-. :.D6i voi =:: : :.- : ' :€:r rong m6t khoing pff nfrfi dinh t") thuQc vio firng enzim O M TA I LI E - Dur.s H dinh mrlc dung tfch lit TA va'o binh IL C l:.-CO k-ha-n h.€n cdn phan tich rdi chuydn ST _.:_= IE :1: :: :crat 0,5M - Dung ;-:. C:- U O M TA IL I ,l:- r - .-: icloaxetic trOn cAn kf thuet, rdi chuydn sang bini dinh mric dung tfch ::r:: '-: -.:= :rudc c{t t6i vach mric, 15c ddu v} dem loclft dung dich' - Dune :::.- .-: :-::;'c-dc 1N pha !o:?ulg82,2LIHCI bang nu6c c{tt6i m6t ST C nghi€nc,1:=.=-::.1,;n:5cdrrngdichd0rncfing.nhukhoingpH-uf9bheP_ddq!i€trritenzim' di.m bio