Microarrays an introduction and their applications to plant and medical research Microarray an introduction and their applications to plant and medical research Nelzo C Ereful A presentation on Microa[.]
Microarray: an introduction and their applications to plant and medical research Nelzo C Ereful A presentation on Microarray No copyright is attached with this presentation, just acknowledge the author and the project Outline 1 “Omics” “Omics”organization organizationoverview overview 2.AApartial partial view view of of transcriptomics transcriptomics 3 Steps Stepsin in Microarray Microarrayanalysis analysis 4.Applications Applicationsof ofmicroarray microarray Nelzo Ereful Omics organization: an overview Genome Transcriptome Proteome Metabolome Nelzo Ereful Citrate Cycle Another way of looking at it Taken from Goodacre (2005) Nelzo Ereful Transcriptomics global analysis of gene expression • transcriptome, complete set of RNA transcripts produced by the genome at any one time • examines the expression level of mRNAs in a given cell population, often using high-throughput techniques such DNA microarray technology Nelzo Ereful Transcriptomics Analysis techniques SAGE MPSS cDNA fingerprinting Microarray Nelzo Ereful What is microarray? • Typically a glass slide where DNA molecules are fixed • Allows simultaneous measurement the level of expression of thousands of genes Nelzo Ereful Landscape Slide spot pingroup subarray gene Nelzo Ereful Affymetrix GeneChip Oligonucleotide: •11-25 mer (short oligo) •50-70 mer (long oligo) http://en.wikipedia.org/wiki/Image:Affymetrix-microarray.jpg Nelzo Ereful Applications of microarray Evolutionary and ecological genomics SNP detection Tiling array Microarray Tumor classification Drug Discovery and development Gene expression (stress) Nelzo Ereful