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Dataset of longitudinal analysis of tear cytokine levels, CD4, CD8 counts and HIV viral load in dry eye patients with HIV infection Q1 Q3 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24[.]
Data in Brief ∎ (∎∎∎∎) ∎∎∎–∎∎∎ Contents lists available at ScienceDirect journal homepage: www.elsevier.com/locate/dib Data Article 10 11 12 13 14 15 16 17 Praveen Kumar Balne a,b,1, Rupesh Agrawal a,b,n,1, 18 Veonice Bijin Au c, Bernett Lee c, Eileen Loo a, Louis Tong b,d,e, 19 Arkasubhra Ghosh b,f, Stephen C Teoh g, John Connolly c, 20 h 21 Q1 Petrina Tan 22 Q3 a National Healthcare Group Eye Institute, Tan Tock Seng Hospital, Singapore b 23 Singapore Eye Research Institute, Singapore c Institute of Molecular and Cell Biology, Singapore 24 d Duke-NUS Medical School, Singapore 25 e National University of Singapore, Singapore 26 f GROW Research Laboratory, Narayana Nethralaya Foundation, India g 27 Eagle Eye Centre, Singapore h Jurong General Hospital, Singapore 28 29 30 31 a r t i c l e i n f o abstract 32 Article history: The data presented in this article shows the longitudinal analysis 33 Received December 2016 of tear fluid cytokine profiles, blood CD4 and CD8 counts and HIV 34 Received in revised form viral load in 34 dry eye patients with HIV infection during the 35 21 December 2016 HAART therapy Clinical samples were collected from HIV patients 36 Accepted 27 January 2017 with dry eye disease at the time of presentation to the clinic (visit 37 1), three months (visit 2) and months (visit 3) after the pre38 Keywords: sentation At each time point tear samples were evaluated for 41 39 Dry eye disease cytokines using Luminex bead based multiplex assay and blood Cytokine profiles 40 samples were tested for HIV viral load and CD4 and CD8 counts HIV infection and longitudinal study 41 & 2017 The Authors Published by Elsevier Inc This is an open 42 access article under the CC BY license 43 (http://creativecommons.org/licenses/by/4.0/) 44 45 46 47 48 n Corresponding author at: National Healthcare Group Eye Institute, Tan Tock Seng Hospital, 11 Jalan Tan Tock Seng, Singapore 49 Q2 308433, Singapore Fax: ỵ 65 63577726 E-mail address: rupesh_agrawal@ttsh.com.sg (R Agrawal) 50 Joint first authors 51 52 http://dx.doi.org/10.1016/j.dib.2017.01.014 53 2352-3409/& 2017 The Authors Published by Elsevier Inc This is an open access article under the CC BY license 54 (http://creativecommons.org/licenses/by/4.0/) Data in Brief Dataset of longitudinal analysis of tear cytokine levels, CD4, CD8 counts and HIV viral load in dry eye patients with HIV infection Please cite this article as: P.K Balne, et al., Dataset of longitudinal analysis of tear cytokine levels, CD4, CD8 counts and HIV viral load in dry eye patients with HIV infection, Data in Brief (2017), http://dx.doi.org/10.1016/j.dib.2017.01.014i P.K Balne et al / Data in Brief ∎ (∎∎∎∎) ∎∎∎–∎∎∎ 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 Specifications Table Subject area More specific subject area Type of data How data was acquired Data format Experimental factors Experimental features Data source location Data accessibility Biology Tear cytokine profiles in dry eye patients with HIV infection Table Luminex assay using the Milliplexs MAP human cytokine/chemokine magnetic bead panel -1 kit with FlexMAP 3D (Luminexs) platform Raw Tear and blood samples collected from dry eye patients with HIV infection at three different time points At each time point tears were analyzed for 41 cytokines using Luminex bead based multiplex assay and systemic CD4, CD8 counts and HIV viral loads were tested using Trucount tubes and qRT-PCR respectively Milliplexs MAP human cytokine/chemokine magnetic bead panel -1 kit (Millipore, USA) with FlexMAP 3D (Luminexs) platform was used for cytokine profiling in tear samples Singapore Data are with this article Value of the data In our earlier reports cytokines IFN-gamma-inducible protein 10 (IP-10, CXCL10), epidermal growth factor (EGF) and growth-regulated oncogene (GRO) levels were significantly altered in dry eye patients with human immunodeficiency virus (HIV) infection compared to immunocompetent dry eye patients at the time of presentation (visit 1/baseline) to the clinic [1] and the dataset of baseline cytokine levels and clinical parameters were reported [2] Here we report the longitudinal tear cytokine profiling data of HIV patients with dry eye disease over the period of months with two follow ups (3 months each) and its association with the systemic CD4 (cluster of differentiation 4), CD8 counts and HIV viral loads This longitudinal data will help researchers to understand the changes in tear cytokines in HIV patients with dry eyes over the time and its association with systemic and viral factors and study further on HIV associated ocular inflammation and its pathogenesis Data The data presented herein was obtained from longitudinal analysis of tear cytokine profiles in 34 dry eye patients with HIV infection at time points (Table 1) Systemic CD4 and CD8 counts and HIV viral loads (Table 2) were also analyzed at each time point to elucidate the association of HIV, systemic and ocular factors in dry eye disease Experimental design, materials and methods The data herein was obtained from HIV patients who had complaints of symptoms related to dry eyes (foreign body sensation, dryness or roughness and irritation) with a prior informed consent and were evaluated following the guidelines of the International Dry Eye Workshop (DEWS), 2007 [3] HIV diagnosis was confirmed by Western blot assay (Diagnostic Biotechnology HIV Blot 2.2) and the severity of the dry eye was evaluated at the time of presentation using different clinical tests as mentioned in the earlier report [1] Thirty four HIV patients with dry eyes were enrolled for the Please cite this article as: P.K Balne, et al., Dataset of longitudinal analysis of tear cytokine levels, CD4, CD8 counts and HIV viral load in dry eye patients with HIV infection, Data in Brief (2017), http://dx.doi.org/10.1016/j.dib.2017.01.014i P.K Balne et al / Data in Brief ∎ (∎∎∎∎) ∎∎∎–∎∎∎ 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 longitudinal analysis and blood and tear samples were collected at time points (at the time of presentation to the clinic (visit 1), months (visit 2) and months (visit 3) after the presentation) Out of 34 patients, one patient died and two patients were withdrawn from the study after the first visit Eight patients were missed the visit and two patients were missed the visit At each time point tear samples were collected using Schirmer's strips and 10 mL of blood was collected aseptically through vein puncture and transferred into an anticoagulant ethylenediamine tetraacetic acid (EDTA) containing tube Tear fluid was eluded from Schirmer's strips as mentioned earlier [1] and stored at 80 °C until cytokine analysis [4] At each time point HIV viral load and blood CD4 and CD8 counts (Table 2) were determined by quantitative real time reverse transcriptase polymerase chain reaction (qRT-PCR) and Trucount tubes (BD Biosciences, San Jose, CA, USA) according to the manufacturer's instructions respectively Schirmer's test was done on HIV patients at each visit (Table 2) and tear cytokine profiling was done at each time point by Luminex bead based multiplex assay with a panel of 41 analytes (Table 1) using Milliplexs MAP human cytokine/chemokine magnetic bead panel kit (Millipore, USA) following the manufacturer's instructions Acknowledgements The research work was support by the Singapore Eye Research Institute Pilot Grant and Tan Tock Q4 Q5 Seng Hospital Pitch for Fund Q6 Transparency document Supplementary material Transparency data associated with this article can be found in the online version at http://dx.doi org/10.1016/j.dib.2017.01.014 Appendix A Supplementary material Supplementary data associated with this article can be found in the online version at http://dx.doi org/10.1016/j.dib.2017.01.014 References [1] R Agrawal, P.K Balne, A Veerappan, V.B Au, B Lee, E Loo, A Ghosh, L Tong, S.C Teoh, J Connolly, P Tan, A distinct cytokines profile in tear film of dry eye disease (DED) patients with HIV infection, Cytokine 88 (2016) 77–84 [2] P.K Balne, R Agrawal, V.B Au, B Lee, E Loo, L Tong, A Ghosh, S.C Teoh, J Connolly, P Tan, Dataset of tear film cytokine levels in dry eye disease (DED) patients with and without HIV infection, Data Brief 10 (2017) 14–16 Q7 [3] Anon., The definition and classification of dry eye disease: report of the Definition and Classification Subcommittee of the International Dry Eye WorkShop, Ocul Surf (2007) 75–92 [4] K.R VanDerMeid, S.P Su, K.L Krenzer, K.W Ward, J.Z Zhang, A method to extract cytokines and matrix metalloproteinases from Schirmer strips and analyze using Luminex, Mol Vis 17 (2011) 1056–1063 Please cite this article as: P.K Balne, et al., Dataset of longitudinal analysis of tear cytokine levels, CD4, CD8 counts and HIV viral load in dry eye patients with HIV infection, Data in Brief (2017), http://dx.doi.org/10.1016/j.dib.2017.01.014i ... dry eyes were enrolled for the Please cite this article as: P.K Balne, et al., Dataset of longitudinal analysis of tear cytokine levels, CD4, CD8 counts and HIV viral load in dry eye patients with. .. Balne, et al., Dataset of longitudinal analysis of tear cytokine levels, CD4, CD8 counts and HIV viral load in dry eye patients with HIV infection, Data in Brief (2017), http://dx.doi.org/10.1016/j.dib.2017.01.014i... Tan, Dataset of tear film cytokine levels in dry eye disease (DED) patients with and without HIV infection, Data Brief 10 (2017) 14–16 Q7 [3] Anon., The definition and classification of dry eye disease: