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Tsp chi Khoa hgc DHQGHN, Khoa hpc Ty nhien va Cong nghe Tap 29, So 4 (2013) 29 35 hoa quy tnnh phan tfch dong thdi cac anion v6 ca trong man nude moi trudng sir dung he didn di mao quan van hanh bang[.]
Tsp chi Khoa hgc DHQGHN, Khoa hpc Ty nhien va Cong nghe Tap 29, So (2013) 29-35 hoa quy tnnh phan tfch dong thdi cac anion v6 ca man nude moi trudng sir dung he didn di mao quan van hanh bang tay ket hop vdi detecto dan khong tiep xuc Nguyen Bich N go c\ Nguyin Kim Diem Mai^'^ Duong Hong Anh‘, Pham Hung Viet'’* ' Trung tarn Nghiiit cihi Cong ngh? Moi tnicmg va Phdt triin Ben viing, Tntcmg Dgi hQc Khoa hge Tu nhien, DHQGHN, 334 Nguyen Trai, Hd Ngi, Viet Nam ^Tntdng Dgi hpc Su Pham Tp.Ho Chf Minh N h |n n g ly 05 thing l i n5m 2013 Chinh sua n g iy 19 thing 11 nSm 2013; ch ip nhan d ingn gay 16 thing 12 nim 2013 Tom tSt: Quy trinh phan ti'ch dong thdi ca phot phat va cic anion co ban khic nude nhir clonia nitrat, nitrit va sunphat nude su dyng he dipn di mao ^uan van hanh bing tay kit hop detector dan khdng tiep xuc da dupe tdi iru hda Cic dieu kidn toi uu cho qui trinh phan tfch la: hS d^m 50 mM Tris/50 mM MOPS d pH 7,7, mao quan PEEK va ip the -15kV Gidi han phit hi|n cua c i nim anion dSu tuong ddi thip tii din 15 pM vdi dO Up (5 -10%) va dp thu h6i (95* 112%) tot Ket qua phin tfch mot so miu thpc cho thay tuong quan cao giOa phuong phip dien di mao quan da phat trien va phuong phip doi chung bing sic k;^ ion Tir kh6a: Dien di mao quan, cam bien d$ din khong tiep xUc anion v6 co figt v in d l Vdi d |c tinh phan tich cac chat tan dua CrSn di£n tfch, tit rat som, thu& dien di (Capillary electrophoresis - CE) da duoc nghien cihi de umg dyng phan tfch cac ion v6 ca Ban dau, nguai ta chti ylu sir dung detector quang, den cuoi nhirng nam 1990, detector do d in khdng tiep xuc (C^D) doi da md rong kha nang ip dung CE cho phan tfch cic anion vJi cation v6 co moi truemg nuac [1] Cac ■ Tic gia lien bS DT: 84-913572589 E-mail: phamhungviet@hus edu.vn nghiSn cihi ciia Kuban [2], Mai Thanh Due [3] da dua quy trinh phin tfch dong thdi cic anion co ban nhir CP, NO2', NOj', S04'', F Tuy nhiln, cac nghien curu niy diu chira de cip toi viec dinh luong phot phIt, mot chi tieu quan trong cac quy chuan k j ihuSt Viet Nam ve chit luong nuoc m |t (QCVN 08:2008/BTNMT) va nude thai (QCVN 14:2008/BTNMT QCVN 24:2008/BTNMT (JCVN 28:2008/BTNMT), Muc lieu cua nghien cuu la dua dugre quy trinh phan tfch dong thdi cac anion cloma, nitnt, nitrat, sunphat va phdt phat tren he thiet N B Nggc va rnik /Tap chi KIwa hoc DHQGHN, Wioa hoc Tir ithten va Cong nghS, Tap 29 Sff4 (2013) 29-35 bi dien di mao quan tir che lao sir dung detector do dan khSng tilp xuc, gidp dira mot giai phdp phan tfch don gian, chi phi thap va hieu qua cao cho cdc tram quan tide chat lugng mrdc hien Phuung phap nghien cihi 2.1 Vgi lieu Todn bq hoa chat duqc sii dung deu thuoc loai tinh khiit phSn lich mua tu Merck (Darmstadt, Germany) hoSc Fluka (Buchs, Switzerland) Dung dich goc 100 mmol/L cua cac anion v6 ca duoc pha tir muoi natri ran tuong ling, rieng NOi' duoc pha lai tir dung djeh goc 1000 ppm (Merck) Cac dung djeh goc ndy sau duqc sir dung de pha m lu chuan Dung dich dem duqc pha ngay, sieu Im loai bo bot trude sir dung Tat ca dem, mau chuan va m lu gia deu duqc pha nuoc deion 2.2 KyihugTdo H f thiet bi dien di mao quan duqc su dung nghign cuu la he thiet bj tu lap d |t voi su dung bq phat nguon cao the EMCO (t6i da ±15 kV) v i detecto dq din khong tiep xiic tu che t?o noi voi bq ghi Pico ADC-16 D ira cue noi dat duqc d |t a dau bom mau Detecto duqc dat o gin diu dien cue cao the Diin the lich su dung la -15 kV Nghiin cuu duqc thuc hien tren hai loai mao quan l i mao quan silica v i mao quan PEEK (poly(etheretherketone)) dSu c6 dudng ki'nh 50 pm chieu d ii 60 cm va chieu dai hiqu dung la 53cm Mao quan silica duqc xu 1^ voi NaOH IM 10 phiic va nude deion 10 phut Con mao quan PEEK khong phai xu ly Mau duqc bom theo nguyen tic xiphong o dq cao chinh lech 16 cm 20 giay, Qua trinh tdi m i h6a duqc thuc hira theo phuong phap toi uu lin luqt timg ySu td: pH (thinh phin dem), ndng dem va lua chqn mao quan C ic thong s6 duqc su dung de so sanh ket qua cua cac difiu kien la diqn ti'ch pic, thdi gian luu v i dq phan giai giua cic pic Sau tdi uu, phuong phip phan tich duqc dinh g ii thdng qua gidi han phat hifn - LOD (S/N = 3), hi§u suit thu hdi va dq lap lai Phuong phap cung duqc i p dung de phan tich cac mau nude mdi trudng lay tir cac gieng nude ngim tai xa Van Phuc, H i Ddng, H i Nqi va so sinh k it qua phan ti'ch thu duqc bing phuong phip sic ky ion Kit qua thao luan 3.1 Phdl trien quy trinh a) Lua chon dim c6 p H phit hap Trong nghiSn cuu niy, bon h? dem d pH khic duqc su dung de khao s it anh hudng len su phan tich cua cic anion li: be dem histidine (12 mM) duqc dieu chinh pH bing axit axetic (His/Ace) den pH 4,0; he ddm MOPS - axit 3-(N-morpholino)propanesulfonic va Histidine (MOPS/His) d ty le I/l vdi pH 5,8; he dem MES-axit 2-(7^-morpholino) ethanesulfonic va Histidine (MES/His) duqc pha d ty le I/l c6 pH 6,5; va he dqm Tris - Tris(hydroxymethyl) aminomethane v i MOPS (Tris/MOPS) cung d ty le 1/1 vdi pH 7,7 D6i vdi cic he dem c6 pH > 4, CTAB (hexadecyltrimethylamraonium bromide) duqc them vio d l diiu chinh ddng EOF Cac ion Cl', SO*^', NOs', NO:' duqc chuin bj d ndng dq 100 |lM rieng ion HPO^"' duqc chuan bj d ndng dq 250 |iM C ic dien di duqc trinh biy hinh Trong dieu kien thf nghiem, chi c6 hai h? dem His/Ace v i Tris/MOPS cho phin tich tot N B Ngpc va tmk /Tap chi Khoa hoc DHQGHN, KJwa hgc Tn nhien va Cdng nglii, TSp 29, So (2013) 29-35 giixa cic pic Tuy nhiSn, dem Tris/MOPS c