evolution of antimicrobial resistance and serotype distribution of streptococcus pneumoniae isolated from children with invasive and noninvasive pneumococcal diseases in algeria from 2005 to 2012

Accepted Manuscript Evolution of antimicrobial resistance and serotype distribution of Streptococcus pneumoniae isolated from children with invasive and non-invasive pneumococcal diseases in Algeria from 2005 to 2012 Nadjia Ramdani-Bouguessa, Hanifa Ziane, Souad Bekhoucha, Zehor Guechi, Amina Azzam, Djamila Touati, Malek Naim, Sihame Azrou, Moufida Hamidi, Abdelouahab Mertani, Abdennour Laraba, Tahar Annane, Salah Kermani, Mohamed Tazir PII: S2052-2975(15)00030-X DOI: 10.1016/j.nmni.2015.02.008 Reference: NMNI 29 To appear in: New Microbes and New Infections Received Date: 19 September 2014 Revised Date: 19 December 2014 Accepted Date: 24 February 2015 Please cite this article as: Ramdani-Bouguessa N, Ziane H, Bekhoucha S, Guechi Z, Azzam A, Touati D, Naim M, Azrou S, Hamidi M, Mertani A, Laraba A, Annane T, Kermani S, Tazir M, Evolution of antimicrobial resistance and serotype distribution of Streptococcus pneumoniae isolated from children with invasive and non-invasive pneumococcal diseases in Algeria from 2005 to 2012, New Microbes and New Infections (2015), doi: 10.1016/j.nmni.2015.02.008 This is a PDF file of an unedited manuscript that has been accepted for publication As a service to our customers we are providing this early version of the manuscript The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain ACCEPTED MANUSCRIPT Evolution of antimicrobial resistance and serotype distribution of Streptococcus pneumoniae isolated from children with invasive and non- invasive pneumococcal diseases in Algeria from 2005 to 2012 Nadjia Ramdani-Bouguessaa, Hanifa Zianea, Souad Bekhouchab, Zehor Guechic, Amina Azzamd, Djamila Touatie, Malek Naimf, Sihame Azroug, Moufida Hamidih, Abdelouahab Mertania, Abdennour Larabai, Tahar Annanei, Salah Kermanij, Mohamed Tazira SC RI PT M AN U a Service de Microbiologie, Centre Hospitalier Universitaire Mustapha Bacha, Place 11 du 1er Mai, Sidi M'Hamed, 16000 Algiers, Algeria Emails: NRB, AM: 12 nramdanidz@yahoo.fr; HZ: h.ziane@hotmail.fr; MT: mohamedtazir@hotmail.com 13 b Centre Hospitalier Universitaire d’Oran, Boulevard du Dr Benzerdjeb, 31000, Oran 14 Algiers, Algeria Emails: SB: sylviamare2000@yahoo.fr 15 c Centre Hospitalier Universitaire Nafissa Hamoud, Rue Boudjemaa Moghni, 16040 16 Hussen Dey, Algiers, Algeria Emails: ZG: zehorguechi@yahoo.fr 17 d Centre Hospitalier Universitaire Nedir Mohamed, Rue Lamali Ahmed, 15000, Tizi 18 Ouzou, Algeria Emails: AA: aminaazzams@yahoo.fr 19 e Centre Hospitalier Universitaire Issad Hassani, Rue Ibrahim Hadjrass, Béni- 20 Messous, 16206, Algeria Emails: DT: touati_jamila@yahoo.fr 21 f Hôpital Central de l'Armée Mohamed Seghir Nekkache, BP 244, 16205, Ain Naadja, 22 Algiers, Algeria Emails: MN: naimlek@hotmail.com 23 g Hôpital de Boufarik, Rue des frères Hamadouche, 09400, Boufarik, Blida, Algeria 24 Email: SA: azrou1981@yahoo.fr AC C EP TE D 10 ACCEPTED MANUSCRIPT h Hôpital Salim Zmirli, Route de Baraki, BP 71, 16200, El Harrach, Algiers, Algeria 26 Email: MH: moufhamidi@gmail.com 27 i Centre Hospitalier Universitaire Lamine Debaghine, Boulevard Said Touati, 16009, 28 Bab El Oued, Algiers, Algeria Email: AL, TA: laraba.abdennour@yahoo.fr 29 j Hopital Ain Taya, Hai si El Houas, 16611, Ain Taya, Algiers, Algeria Email: SK: 30 salahkermani@yahoo.fr 31 Corresponding author: Nadjia Ramdani-Bouguessa 32 Phone: +213 21 23 57 87 SC RI PT 25 AC C EP TE D M AN U 33 ACCEPTED MANUSCRIPT Abstract 35 Pneumococcal infections are a major cause of morbidity and mortality in developing 36 countries The introduction of pneumococcal conjugate vaccines (PCVs) has 37 dramatically reduced the incidence of pneumococcal diseases PVCs are not 38 currently being used in Algeria We conduct a prospective study from 2005 to 2012 in 39 Algeria to determine antimicrobial drug resistance and serotype distribution of 40 Streptococcus pneumoniae from children with pneumococcal disease 41 Among 270 isolated strains from children, 97 (36%) were invasive disease; of which 42 48% were not susceptible to penicillin, and 53% not susceptible to erythromycin A 43 high rate of antimicrobial non-susceptibility was observed in strains isolated from 44 children with meningitis The serotype distribution from pneumococci isolated from 45 children with invasive infections was (by order of prevalence): 14, 1, 19F, 19A, 6B, 5, 46 3, 6A, and 23F Multidrug resistance was observed in serotypes 14, 19F, 19A and 47 6B The vaccine coverage of serotypes isolated from children