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[...]...Cellular Respiration • Stages of respiration: 1 Glycolysis – prep carbons Cellular Respiration 1 Glycolysis • 1 glucose (6C) 2 pyruvate (3C) • key points: - inputs - ATP - NAD+/NADH - CO2 and H2O - outputs • eukaryotes AND prokaryotes Glycolysis Cellular Respiration -inputs: -outputs: Where do they go? Cellular Respiration Coenzyme Junction • 2 pyruvate (3C)... Chemiosmosis ATP produced! Cellular Respiration 4 Chemiosmosis • ATP synthase: inner mitochondrial membrane • energy input ATP – H+ gradient • chemiosmosis – ion gradient to do work 4 Chemiosmosis Cellular Respiration • Four parts to ATP synthase: Rotor, Stator, Rod, Knob • H+ must enter matrix here • Generates 1 ATP per ~3.4 H+ Cellular Respiration Summary of respiration • Cells convert ~ 40% of energy... gasoline energy Cellular Respiration - anaerobic • no O2 – no oxidative phosphorylation • fermentation - extension of glycolysis • substrate-level phosphorylation only • need to regenerate e- carrier (NAD+) Cellular Respiration - anaerobic Cellular Respiration - anaerobic • Types of fermentation 1 alcohol • pyruvate converted to acetaldehyde • acetaldehyde accepts e• ethanol produced Cellular Respiration. .. Steps of Respiration • Steps of respiration: 1 glycolysis - cytosol Coenzyme Junction 2 Citric acid cycle - mitochondrial matrix 3 ETC - inner mitochondrial membrane 4 Chemiosmosis - inner membrane to intermembrane space Steps of Respiration • Stages of respiration: 3 ETC Proton Motive Force Substrate-level phosphorylation Phosphate group moved from substrate to ADP yields ATP Cellular Respiration. .. phosphorylation Cellular Respiration – mitochondria revisited Cellular Respiration Electron transport chain (ETC) • ETC e- collection molecules • embedded on inner mitochondrial membrane • accept e- in turn • e- ultimately accepted by O2 (O2 reduced to H2O) Electron transport chain (ETC) -inputs: per glucose, -outputs: Where do they go? H+ NAD+/FAD Steps of Respiration • Stages of respiration: 4 Chemiosmosis... Acetyl CoA (2C) Steps of Respiration • Stages of respiration: 2 Citric acid cycle e- transfer: redox Cellular Respiration 2 Citric acid cycle • few ATP so far • mitochondrial matrix • 2 Acetyl CoA (2C) join oxaloacetate (4C) • 2 citrate (6C) converted several steps, 4C lost (CO2) • e- to carriers (NAD+, FAD) Citric acid cycle -inputs: -outputs: Where do they go? Self-Check Step of Respiration Inputs Outputs... alcohol • pyruvate converted to acetaldehyde • acetaldehyde accepts e• ethanol produced Cellular Respiration - anaerobic • Types of fermentation 2 Lactic acid • pyruvate accepts e- • lactate produced Cellular Respiration - anaerobic • inputs/outputs • alcohol • pyruvate in • CO2 and EtOH out • brewing & baking • lactic acid • pyruvate in • lactate out • muscle fatigue . (3C)
• key points: - inputs
- NAD+/NADH
- ATP
Cellular Respiration
- CO
2
and H
2
O
- outputs
• eukaryotes AND prokaryotes
-inputs:
-outputs:
Where do. for respiration
• transfer of e- to oxygen is stepwise
Redox Reactions
• e- moved by NAD+ (niacin)
• when “carrying” e- (& H+), NADH
Where do e- come