VIỆN ĐẠI HỌC MỞ HÀ NỘI KHOA CÔNG NGHỆ SINH HỌC fOfoOoscjJ KHÓA LUẬN TỐT NGHIỆP Đề tài Thư viện Viện £)ạj học Mở Hà Nội Phân lập nấm mốc chịu nhiệt, chịu pH thấp Ngưòi hưóng dẫn PGS TS Vũ Nguyên Thành.
VIỆN ĐẠI HỌC MỞ HÀ NỘI KHOA CÔNG NGHỆ SINH HỌC fOfoOoscjJ KHÓA LUẬN TỐT NGHIỆP Đề tài: Thư viện Viện £)ạj học Mở Hà Nội Phân lập nấm mốc chịu nhiệt, chịu pH thấp Ngưịi hưóng dẫn: PGS.TS.Vũ Ngun Thành Sinh viên: Hồng Văn Năm Niên khóa: K20 - Lóp 1301-CNSH Viện đại học Mở Hà Nội Khoa Cơng nghệ sinh học DANH MỤC HÌNH Hình 1.1 Cấu trúc thành phan cùa lignoecllulose ngơ Error! Bookmark not defined Hình 1.2 Cấu trúc phân tứ cellulose [3] Error! Bookmark not defined Hình 1.3 Arabino-4-O-methylglucuronoxylan từ hạt trần (theo Dekker 1985) Error! Bookmark not defined Hình 1,4.Hoạt động xúc tác cùa cellulase chat cellulose [ ].Error! Bookmark not defined Hình 3.1 Đĩa phân lập mẫutrên mơi trường giấy-glucose rơm pH 2.0, nuôi 50 Error! Bookmark not defined Hình 3.2 Đĩa phân lập mẫutrên môi trường giấy-glucose rơm pH 2.0, nuôi Ở 50 sau ngày .Error! Bookmark not defined Hình 3.3 Khuẩn lạc Talaromyces atroroseusLPH 263-1 mặt trước mặt sau đĩa thạch PDAtrong ngàyờ 30 Error! Bookmark not defined Hình 3.4 Hình thái tế bào chùng Talaromyces atroroseus LPH 263-1, kích thước vật kính 40xni ngày môi trường PDA Error! Bookmark not defined Thư viện Viện Đại học Mở Hà Nội Hình 3.5.Khuấn lạc Rhizomucor pusillusLPH 239 mặt trước mặt sau, sau ngày nuôi môi trường PDA Error! Bookmark not defined Hình 3.6 Hình thái tế bào chúng Rhizomucor pusillus LPH 239 nuôi ngày PDAsoi vật kính 40x,kích thước trèn tương ứng lOum Error! Bookmark not defined Hình 3.7 Khuấn lạc Penicillium simplicissimum LPH 260 mặt trước mặt sau, sau ngày nuôi môi trường PDA Error! Bookmark not defined Hình 3.8 Hình tháitế bào Pcnicillium simplicissimum LPH 260 ni ngày môi môi trường PDA, soi vật kính 40x, kích thước chèn tương ứng 10pm Error! Bookmark not defined Hình 3.9 Bicu đồ hoạt tính CMCase chủng chịu nhiệt (50 °C) pH khác Error! Bookmark not defined Hình 3.10 Biếu đồ hoạt tính CMCase chúngnấm mốc (30 °C) pH khác Error! Bookmark not defined Hoàng Văn Năm Pageii Viện đại học Mở Hà Nội Khoa Công nghệ sinh học Hình 3.11 Hoạt tính xylanase nấm mốc chịu nhiêt (pH 2.0,50 ) phân tích pH 3.0, pH 5.0, pH 7.0 Error! Bookmark not defined Hình 3.12 Hoạt tính xylanase chùng nấm mốc chịu pH thấp (pH 1.0, 30 °C) phân tích Ớ pH 3.0, pH 5.0, pH 7.0 Error! Bookmark not defined Hình 3.13 Khả bền nhiệt CMCase chủng nấm mốc chịu nhiệt chịu nhiệt (pH 2.0, 50 °C) Error! Bookmark not defined Hình 3.14 Bicu đồ khả ben nhiệt CMCase chúng chịu pH thấp ( pH 1.0.0, 30 °C) Error! Bookmark not defined Hình 3.15 Biếu đồkhà bền nhiệt xylanase chùng nấm mốc chịu nhiệt (pH 2.0, 50°C) Error! Bookmark not defined Hình 3.16.Biêu đồ bền nhiệt xylannase chúng nấm mốc chịu pH thấp (30 °C, pH 1.0) Error! Bookmark not defined Hình 3.17 Điện di SDS-PAGE cúa 40 chủng nam mốc chịu nhiệt Error! Bookmark not defined Hình 3.18 Phố fingerprinting chúng nấm mốc phân lập Error! Bookmark not defined Thư viện Viện Đại học Mở Hà Nội Hoàng Văn Năm Pageiii Viện đại học Mở Hà Nội Khoa Công nghệ sinh học DANH MỤC BẢNG Bàng 1.1 Thành phàn lignocellulose số nguồn có tự nhiên [81 .Error! Bookmark not defined Bảng 1.2 Phân nhóm dựa vào phổ fingerprinting cúa 52 chúng nấm mốc Error! Bookmark not defined Báng 1.3 Danh sách tên chùng đọc trình tự Error! Bookmark not defined Thư viện Viện Đại học Mở Hà Nội Hoàng Văn Năm Pageiv Viện đại học Mở Hà Nội Khoa Công nghệ sinh học DANH MỤC BẢNG PHỤ LỤC Bàng phụ lục 1.1 Bàngnguồn gốc phân lập chủng nấm mốc Error! Bookmark not defined Bảng phụ lục 1.2 Ký hiệu nguồn gốc mầu Error! Bookmark not defined Bảng phụ lục 1.3 Hoạt tính CMCasecủa chủng chịu nhiệt (pH 2.0, 50 °CỚ pH 3.0, pH 5.0, pH 7.0 .Error! Bookmark not defined Báng phụ lục 1.4 hoạt tính CMC chùng nấm mốc chịu pH thấp (pH 1.0, 30°C) Error! Bookmark not defined Bảng phụ lục 1.5 hoạt tính xylannase chúng nấm mốc chịu nhiệt(pH 2.0, 50 °C) ở pH 3.0, pH 5.0, pH 1.0 Error! Bookmark not defined Bảng phụ lục 1.7 Khả bền nhiệt CMCase chủng nấm mốc chịu nhiệt (pH 2.0, 50°C) Error! Bookmark not defined Bảng phụ lục 1.8 khả bền nhiệt CMCase cũa chúng nấm mốc chịu pH thấp ( pH 1.0, 30 °C) Error! Bookmark not defined Bảng phụ lục 1.9 Khả bền nhiệt xylannase cùa chùng chịu nhíệt(pH 2.0, 50 °C), Ờ pH 5.0 |q 0.^.ị Ị:|0£ JỤ].^ Error! Bookmark not defined Bàng phụ lục 1.10 Khá bền nhiệtxylannase chủng chịu pH thấp ( pH 1.0, 30 °C) Ở pH 5.0 Error! Bookmark not defined Mã trình tự gen cùa chủng nấm mốc phân lập Error! Bookmark not defined Hoàng Văn Năm Pagev Viện đại học Mở Hà Nội Khoa Công nghệ sinh học DANH MỤC CHŨ VIÉT TẤT APS CMC DNA DNS dNTPs GH ITS IU kDa OD PCR PDA rDNA Ammonium persulfate Carboxyl methyl cellulose Deoxyribonucleic Acid 3,5 Dinitrosalicylic acid Deoxyribonucleotide triphosphates Glycoside hydrolase Internal transcribed spacer International unit KiloDalton opical Density Polymerase Chain Reaction Potato dextrose agar Ribosomal Deoxyribonucleic Acid SDS-PAGE Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophorosis Thư viện Viện Đại học Mở Hà Nội Hồng Văn Năm Pagevi Khoa Cơng nghệ sinh học Viện đại học Mở Hà Nội MỤC LỤC LƠI CAM ƠN Error! Bookmark not defined DANH MỤC HÌNH Error! Bookmark not defined DANH MỤC BANG Error! Bookmark not defined DANH MỤC BANG PHỤ LỤC Error! Bookmark not defined DANH MỤC CHỪ VIẾT TẢT Error! Bookmark not defined MỤC LỤC Error! Bookmark not defined Mơ ĐÀU Error! Bookmark not defined PHÂN TỎNG QUAN Error! Bookmark not defined 1.1 1.1.1 1.1.2 1.2 1.2.1 Giới thiệu lignocellulose Error! Bookmark not defined Cellulose Error! Bookmark not defined Hemicellulose Error! Bookmark not defined Enzyme thủy phân Lignocellulose Error! Bookmark not defined .Enzyme thủy phân cellulose j.; Error! Bookmark not defined 1.2.2.Enzyme thủy phân hemicellulose Error! Bookmark not defined 1.3 ứng dụng enzyme thúy phân lignocellulose Error! Bookmark not defined 1.4 Những nghiên cứu enzyme thủy phân lignocenlulose Error! Bookmark not defined PHÀN 2: PHƯƠNG PHÁP NGHIÊN cứu Error! Bookmark not defined 2.1 Đối tượng nghiên cứu .Error! Bookmark not defined 2.2 Hóa chất thiết bị Error! Bookmark not defined 2.2.1 Hóa chất Error! Bookmark not defined 2.2.2 Thiết bi Error! Bookmark not defined 2.3 Môi trường nghiên cứu Error! Bookmark not defined 2.4 Phương pháp nghiên cứu: Error! Bookmark not defined 2.4.1 Phương pháp lấy mầu Error! Bookmark not defined 2.4.2 Phương pháp phân lập, nuôi cấy, làm bảo quản chúng giống Error! Bookmark not defined Hoàng Văn Năm Pagevii Viện đại học Mở Hà Nội Khoa Công nghệ sinh học 2.4.3 Quan sát hình thái khuẩn lạc chụp ánh Error! Bookmark not defined 2.4.4 Nuôi cay tách chiết enzyme Error! Bookmark not defined 2.4.5 Xác định hoạt độ enzyme phương pháp DNS: Error! Bookmark not defined 2.4.6 Phương pháp điện di protein SDS-PAGE Error! Bookmark not defined 2.4.7 Tách chiết ADN tổng số Error! Bookmark not defined 2.4.8 Phương pháp PCR fingerprinting Error! Bookmark not defined 2.4.9 Định tên chủng nấm mốc dựa vào giải trình tự DNA Error! Bookmark not defined PHÀN 3: KẼT QUA Error! Bookmark not defined 3.1 Ket phân lập chùng nấm mốc pH thấp Error! Bookmark not defined 3.2 Hình ảnh khuẩn lạc tế bào Error! Bookmark not defined 3.3 Phân tích hoạt tính emzymc bàng phương pháp DNS pHkhác Error! Bookmark not defined 3.3.1 Hoạt tính CMCase Error! Bookmark not defined 3.3.2 Hoạt tính xylanase Error! Bookmark not defined 3.3.3 Xác định bền nhiệt Error! Bookmark not defined 3.3.4 Khà bền nhiệt xylanase Error! Bookmark not defined 3.3.5 Điện di protein SDS-PAGE Error! Bookmark not defined 3.4 Phân nhóm định tên chùng Error! Bookmark not defined 3.5 ĐỊnh tên chủng nấm mốc dựa vào việc đọc trình tự rDNA: Error! Bookmark not defined KẾT LUẬN VÀ KIẾN NGHỊ Error! Bookmark not defined TAI LIẸU THAM KHAO Error! Bookmark not defined PHÀN PHỤ LỤC Error! Bookmark not defined Hoàng Văn Năm Pageviii Viện đại học Mở Hà Nội Khoa Công nghệ sinh học MỞ ĐẦU The giới vi sinh vật xung quanh đa dạng phong phú, chúng tồn phát triền cách mạnh mẽ, có số hữu ích mơi trường người Vì vậy, việc nghiên cứu tìm chúng có ích đem lại lợi ích to lớn cho người xã hội Thực trạng đặc biệt nước nông nghiệp có Việt Nam, tình trạng nhiễm mơi trường việc xà thải phụ phẩmtrong sản phấm nông nghiệp ngày nghiêm trọng Sau mồi đợt thu hoạch nông sản, cách phụ phấm rơm,rạ, bỏ lại đốt chỗ Việc gây ánh hưởng lớn tới môi trường làm tăng lượng khí co? gây hiệu ứng nhà kính, gây tượng sương mù, khói bụi ánh hướng tới sức khỏe người Khơng có vậy, xí nghiệp nhà máy chế biến lâm sàn mọc lên ngày nhiềuđồng nghĩa với việc sàn phấm phụ võ, mùn cưa thải khơng ả&&c\ậtfỈJụn^Jà ìtì^y^ắỊ'lẩ§g^pfị’và nhiễm mơi trường Thành phần phụ phẩm chứa hàm lượng lignocellulose lớn cấu trúc lại bền khó bị phân húy vi sinh vật Vì lý mà việc sử dụng enzyme thủy phân lignocellulose đe xử lý phụ phẩm nông nghiệp sê làm giảm ô nhiễm môi trường đồng thời tận dụng chúng ũ làm thức ăn cho gia xúc (rơm rạ) hay làm phân bón cho trồng (gỗ) Đặc biệt đế nâng cao trình chuyển hóa thức ăn cho vật ni giúp vật ni sinh trưởng pháttriền mạnhta cần bố xung enzyme thủy phân lignocellulose vào thức ăn song trình sàn xuất công đoạn ép viên cần nhiệt độ cao từ 70°C- 80 °Cvà dày động vật có độpH thấp enzyme sứ dụng phái hoạt động nhiệt độ cao pH thấp Hoàng Văn Năm Pagel Viện đại học Mở Hà Nội Khoa Cơng nghệ sinh học Nhận thấy lợi ích tầm quan trọng đó, chúng tơi tiến hành tìm kiếm, nghiên cứu chúng nấm mốc có khả sinh enzyme thuỳ phân mạnh nguồn giàu lignocellulose từ môi trường khác nham đáp ứng nhu cầu thực tiền đời sống với đề tài: “Phân lập nấm mốc chịu nhiệt, chịu pH thấp” Muc tiêu: Tìm kiếm, phân lập chủng nấm mốc chịu nhiệt, chịu pH có khã sinh enzyme thúy phân lignocellulose Thư viện Viện Đại học Mở Hà Nội Hoàng Văn Năm Page2 VIỆN ĐẠI HỌC MỚ HÀ NỘIKHOA CÔNG NGHỆ SINH HỌC Bảng phụ lục khả bền nhiệt CMCase cua chúng nấm mốc chịu pH thấp ( pH 1.0, 30°C) Hoạt tính(IU/ml) pH 5.0 Tỉ lệ 70/25 % STT Ký hiệu chủng Tén loài LPH 237 Curvularia eragrostidis 1.32 LPH 238 Trichoderma koningiopsis 1.44 LPH 239 Rhizomucor pusillus 2.7 1.43 189.36 LPH 239-1 Talaromyces purpureogenus 0.99 2.49 39 63 LPH 239-2 Talaromyces solicola 2.55 LPH 240 Aspergillus niger 1.81 2.14 84.4 LPH 241-1 Talaromyces solicola 0.12 LPH 241-2 Talaromyces barcinensis 2.25 05 214.49 LPH 242-1 Aspergillus fumigatus 3.51 1.46 240.63 10 LPH 242-2 Talaromyces solicola 2.07 1.93 107.09 11 LPH 243 Rhizopus oryzae 0.11 12 LPH 244 0 13 LPH 245-1 Talaromyces solicola 0 14 LPH 245-2 ,-p Talarcmyces solicpla 70 °C ‘as/UẶ/Us 11 » r ? 25 °C [ T '°x T * *4.7^ * 0.62 0.97 0 15 LPH 256 16 LPH 257-1 17 LPH 257-2 Trichoderma asperellum 2.7 18 LPH 259 Penicillium oxalicum 0.49 2.31 21.05 176.56 Morỉierella wolfii 19 LPH 260 Penicillium simplicissimum 0.09 0.46 20 20 LPH 262 Aspergillus ỉerreus 1.25 2.92 42.71 21 LPH 263 Talaromyces helicus 0.4 1.43 27.66 22 LPH 263-1X Talaromyces atroroseus 1.55 1.49 104.08 23 LPH 263-2T Talaromyces barcinensis 0.71 2.61 27.33 24 LPH 264 Aspergillus niger 2.37 3.01 78.79 25 LPH 265 Acidomyces acidothermus 0 26 LPH 266 Aspergillus aculeatus 0 27 LPH 267 Aspergillus heteromorphus 1.28 1.98 64.62 Hoàng Văn Năm Page68 VIỆN ĐẠI HỌC MỚ HÀ NỘIKHOA CÔNG NGHỆ SINH HỌC Bảng phụ lục Khả bền nhiệt xylannase chủng chịu nhiệt(pH 2.0, 50°C), ỏ- pH 5.0 Hoạt tính(IU/ml) 5.0 pH Ti lệ 70/25 STT Ký hiệu chùng Tên loài LPHT 220 Thermomyces lanuginosus 58.87 92.94 63.34 LPHT 221 Thielavia terrestris 20.63 39.92 51.69 LPHT 222 Thielavia Sp1 20.91 38.97 53.67 LPHT 223 Thermomyces lanuginosus 23.9 62.25 38.4 LPHT 224 16.14 LPHT 225 Thielavia Sp4 43.85 196.03 22.37 LPHT 226 Thielavia Sp1 15.4 43.13 35.69 LPHT 227 Rasamsonia emersonii 0 LPHT 228 Rhizomucor pusillus 0 10 LPHT 230 Thielavia Sp11 43.25 181.35 23.85 11 LPHT 231 12 LPHT 232 13 14 15 LPHT 235 16 LPHT 236 Thielavia spl 17 LPHT 246 18 LPHT 251 19 LPHT 252 25°c 70°C w™ Đại họ 'Mờ ĩ ỉà Nội Thielavia Sp12 10.24 37.1 27.59 LPHT 233 Thielaviaspì5 128.57 340.48 37.76 LPHT 234 Rasamsonia emersonii 76.98 315.08 24.43 137.3 349.6 39.27 Rasamsonia byssochlamydoides 16.59 64.13 25.87 Aspergillus fumigatus 31.04 54.58 56.86 157.14 402.78 39.01 94.07 20 LPHT 253 Thermomyces lanuginosus 28.27 30.06 21 LPHT 254 Aspergillus fumigatus 35.77 61.45 58.2 22 LPHT 258 20.6 94.9 21.71 23 LPHT 268 36.92 51.82 71.26 24 LPHT 269 36.66 40.22 91.13 Hoàng Văn Năm Thermomyces dupontii % Page69 VIỆN ĐẠI HỌC MỚ HÀ NỘIKHOA CÔNG NGHỆ SINH HỌC Bảng phụ lục 10 Khả bền nhiệtxylannase chủng chịu pH thấp( pH 1.0, 30°C) ỏ pH 5.0 STT Ký hiệu chủng Hoạt tính(IU/ml) 5.0 Tên loài 70°C pH Tỉ lệ 70/25 6.33 67.07 85.44 37.01 93.2 39.71 Talaromyces purpureogenus 70.19 Talaromyces solicola 5.35 LPH 240 Aspergillus niger 71.89 LPH 241-1 Talaromyces solicola 2.59 LPH 241-2 Talaromyces barcinensis 32.64 91.6 35.64 LPH 242-1 Aspergillus fumigatus 32.64 91.6 35.64 10 LPH 242-2 Talaromyces solicola 37.91 91.24 41.54 11 LPH 243 Rhizoous oryzae 12 LPH 244 13 LPH 245-1 Talaromyces solicola 0 14 LPH 245-2 Talaromyces solicola 0.36 21.32 1.67 15 LPH 256 Aspergillus flavus 32.02 100.78 31.77 16 LPH 257-1 Mortierella wolfii 17 LPH 257-2 Trichoderma asperellum 0.54 103.1 0.52 18 LPH 259 Penicillium oxalicum 0.54 93.92 0.57 19 LPH 260 Penicillium simplicissimum 58.42 20 LPH 262 Aspergillus terreus 2.5 101.23 2.47 21 LPH 263 Talaromyces helicus 11.15 80.72 13.81 22 LPH 263-1X Talaromyces atroroseus 4.19 85.62 4.9 23 LPH 263-2T Talaromyces barcinensis 13.56 71.26 19.02 0.18 69.12 0.26 0.8 LPH 237 Curvularia eragrostidis LPH 238 Trichoderma koningiopsis LPH 239 Rhizomucor pusillus LPH 239-1 LPH 239-2 I hu V ỈC k s X T1-'0'44 11 V lỹlì Udi lìụu 0.62 L ^1.81 9.44 1.01 ##### 24 LPH 264 Aspergillus niger 25 LPH 265 Acidomyces acidothermus 26 LPH 266 Aspergillus aculeatus 0.36 53.87 0.66 27 LPH 267 Aspergillus heteromorphus 0.89 64.75 1.38 Hoàng Văn Năm % 25°c Page70 VIỆN ĐẠI HỌC MỔ HÀ NỘIKHOA CÔNG NGHỆ SINH HỌC Mã trình tự gen chủng nấm mốc phân lập đưọc >LPH 237 1.5-4 ITS 5-1'1 S4 ITS 600 VT 1542 TGAGAAGAAGGTCTTGATGGATTGCTGGCCTCTTTAGCTGGTTGCAAGCGCAAAAATGTGCTGCGCTCCGAAACC AGTAGGCCGGCTGCCAATCGTTTTAAGGCGAGTCTTTGGGGCAAAAGCCAAAGACAAAACGCCCAACACCAAGCA AAGCTTGAGGGTACAAATGACGCTCGAACAGGCATGCCCTTTGGẦATACCAAAGGGCGCAATGTGCGTTCAAAGA TTCGATGATTCACTGAATTCTGCAATTCACACTACGTATCGCATTTCGCTGCGTTCTTCATCGATGCCAGAACCA AGAGATCCGTTGTTGAAAGTTGTAAATGATTTACATTTGTTATACTGACGCTGATTGCAACTGCATAAAAẦAGGT TTATGGTGTGGTCCTGGTGGCGGGCGAACCCGCCCAGGAAACAACAAGTGCGCAAAAGACATGGGTGAAAAATAA TTCAGCTTCCCCGCCAAAÃCTGGCGGTTGCAGCCTTCATATTTTTTGTGTAATGATCCCTCCGCAGGTTCACCTA CGGAGACCTTGTTAC Cochlioboỉus eragrostidis Sequence ID: JN943412.1 Identities >LPH 238 2.5-4 ITS 5-ITS4 531/531(100%) ITS 600 VT1543 CAGAAGTTGGGTGTTTTACGGACGTGGACGCGCCGCGCTCCCGGTGCGAGTTGTGCAAACTACTGCGCAGGAGAG GCTGCGGCGAGACCGCCACTGTATTTCGGGGCCGGGATCCCGTCTTAGGGGTTCCCGATCCCCAACGCCGACCCC CCGGAGGGGTTCGAGGGTTGAAATGACGCTCGGACAGGCATGCCCGCCAGAATACTGGCGGGCGCAATGTGCGTT CAAAGATTCGATGATTCACTGAATTCTGCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCA GAACCAAGAGATCCGTTGTTGAAAGTTTTGATTCATTTTGAATTTTTGCTCAGAGCTGTAAGAAATAACGTCCGC GAGGGGACTACAGAAAGAGTTTGGTTGGTCCCTCCGGCGGGCGCCTGGTTCCGGGGCTGCGACGCACCCGGGGCG TGACCCCGCCGAGGCAACAGTTTGGTATGGTTCACATTGGGTTTGGGAGTTGTAAACTCGGTAATGATCCCTCCG CTGGTTCACCAACGGAGACCTTGTTAC Trichoderma koningiopsis Sequence ID: KP3402Ỵ2 llỊÌept it ies.:, 552/552 (100^) >LPH 239 Tmr yien yien Dai hoc Mở’Hà Noi 3.5-4 ITS 5-ITS4 ITS 600 VT 1544 TAGTTGAATTTATTTGTGGTTGATTGACCTTTATACTTGATCATCTCGAAGCACACTGGTAAAAAATTAGATACC AATGCAAACCCTCAAGGAAATATCAAGGGTCAACAACATACAAATTGTTCGGGTAGCTTGAACGGATGAAAATCC ATCAGCACCGCAAATCCAAAGATTTATCAAAAAATAAACCTTTAGGGGTTAATAAAGATACTGAACTAGACGTAC CCAATGGATGAACCAAAGGGTGCAAGGTGCGTTCGAGAATTCGATGATTCGCAAAGGCTGCAGATCGCATTACTT TTCGCAATTTGCTACGCTCTTCATCGATGCGAGAACCAAGTGATCCATTGCTTAAAGTTGTTTTGAAATTTTATC GTCTTGAATCATTTCTGATCCAAAACTTTCAATTTGTTAAACAACAGTCAATCAAATATGTAAAATAAAAGGTCG AGAGATCTGGAGCAATCCCTCAGAACATTTCACAAAAAGGAGAGAATCGCGGCTCACCAATAGAGGTCACCACGA TTTCCACAACTTTTTAATGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTA Rhizomucor tauricus Sequence ID: KJ948646 Identities: 552/552(100%) >LPH 239-1 4.5-4 ITS 5-ITS4 ITS 600 VT 1545 CACCGTGGAATAAATTCGTGAGGTGACCAACGCTCCGCGGGTCCÃTCCCGAGCGAGTGACAAAGCCCCATÃCGCT CGAGGACCTGGGCGGGAACGTCGCCGCTGCCTTTCGGGCAGGTCCCCCCGTTTCGGAGGGACCAGCGCCCAACAC ACAAGCCGTGCTTGAGGGCAGAAATGACGCTCGGACAGGCATGCTCCCCGGAATGCCAGGGAGCGCAATGTGCGT TCAAAGATTCGATGATTCACGGAATTCTGCAATTCÃCATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCC GGAACCAAGAGATCCATTGTTGAAAGTTTTGATTATTATCAACGTTGCTCAGACAGTCCATCTTCACAAAGGGTT CAAGGGGCGCTCCGGCGGGCACGGGCCCGAGGACAGAGGCGTCCTCCGGCGACCGGGAGACCCGGTGGGCCCGCC AAAGCAACACGGTATTCAAATAGACACGGGTGGGAGGTTGGACCGCGAGGGGTCCGCACTCGGTAATGATCCTTC CGCAGGTTCACCTACGGAAACCTTGTTA Talaromyces purpureogenus Sequence ID: HQ608123.1 Identities: 505/554 (91%) >LPH 239-2 5.5-4 ITS 5-ITS4 ITS 600 VT 1546 ATGGAAGACCAACGCCCGTAGATCCTTCCCGAGCGGGTGACAGAGCCCCATACGCTCGAGGACCTAGGCGGGCGT CGCCACTGCCTTTCAGGCAGGTCCCCGGGGGGACCACACCCAACACACAAGCCGTGCTTGAGGGCAGAAATGACG CTCGGACAGGCATGCCCCCCGGAATGCCAGGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTG CAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT Hoàng Văn Năm Page71 VIỆN ĐẠI HỌC MỔ HÀ NỘIKHOA CÔNG NGHỆ SINH HỌC TGATTATTATCAATCATGCTCAGACAGTCCATCTTCATGAGGGTTCAAGGGCGCTCTGGCGGGCACGGGCCCAGG GGCACAGGGGCTCCTCGGCGACCGGGAGAGCCCAGTGGGCCCGCCAAAGCAACAGGGTGTACAAGAGACAAGGGT GGGAGGTTCGGTCCACGCGGGACCGGCACTCÃGTAATGATCCTTCCGCAGGTTCACCTACGGAACGGTTAGGTTT CACTTGGGGGGGTTTAGGCCCCAACAGTGTACTCGCCCCCCTGGTCTCCCAGGGAGCCCGACTATATCTTAAGCA GGACTCGACGTCCCACCCACTTCCACTTAGTCTGTGAACGTTCCCCATATCCCCCCTGAAACTACAGGGGACACT TAGGGGCTTCGATGCGGATTACCCATGGTCTCTGCCTGGAGCAGAACTATCCGAATCGTCTGTTACCGTCCCCCG TGGTGTGTCCACGGGCCCCTGCACGGTTTCCCGCGGCAGGTTGGTGCGATCGGCTTTAGGGCGTCCCCGTCAATT CAAAAGTGTCGCCCAGCCCGGGAGGGGCTGGACTTGCAACGGCACCAT Talaromyces soli cola Sequence ID: KF114730.lidentities: 436/444 (98%) >LPH 240 6.5-4 ITS 5-ITS4 ITS 600 VT 1547 CGTCGGCAGGCGCCGGCCAATCCTACAGAGCATGTGACAAAGCCCCATACGCTCGAGGATCGGACGCGGTGCCGC CGCTGCCTTTCGGGCCCGTCCCCCCGGAGAGGGGGACGGCGACCCAACACACAAGCCGGGCTTGAGGGCAGCAAT GACGCTCGGACAGGCATGCCCCCCGGAATACCAGGGGGCGCAATGTGCGTTCAAAGACTCGATGATTCACTGAAT TCTGCAATTCACATTAGTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAA GTTTTAACTGATTGCATTCAATCAACTCAGACTGCACGCTTTCAGACAGTGTTCGTGTTGGGGTCTCCGGCGGGC ACGGGCCCGGGGGGCAGAGGCGCCCCCCCGGCGGCCGACAAGCGGCGGGCCCGCCGAAGCAACAGGGTACAATAG ACACGGATGGGAGGTTGGGCCCAAAGGACCCGCACTCGGTAATGATCCTTCCGCAGGTTCACCTACGGAAACCTT GTT Aspergillus niger Sequence ID: KP724986.lidentities: 528/528(100%) >LPH 241-1 7.5-4 ITS 5-ITS4 ITS 600 VT 1548 (cao) GATGGAAGACCAACGCCCGTAGATCCTTCCCGAGCGGGTGACAGAGCCCCATACGCTCGAGGACCTAG GCGGGCGTCGCCACTGCCTTTCAGGCAGGTCCCCGGGGGGACCACACCCAACACACAAGCCGTGCTTG AGGGCAGAAATGACGCTCGGACAGGCATGCCCCCCGGAATGCCAGGGGGCGCAATGTGCGTTCAAAGA TTCGATGATTCACGGAATTCTGCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCC GGAACCAAGAGATCCAXTGTTGAAAGTTTTGAjCTATTATCAATCATGGTQAGACAGTCCATCTTCATG AGGGTTCAAGGGCGCjHlầác\;^0AOfetíề8ccẰợt(ỈỈỘKcÃơéỈ5GƠÌbLPH 241-2 8.5-4 ITS 5-ITS4 ITS 600 VT 1549 GTAAAAAGATTTGGTGGTGACCAACCCCCGCAGGTCCCTCCCGAGCGAGTGACAAAGCCCCATGCGCTCGAGGAC CCGACGGGCGTCGCCGCTGCCTTTGGGGCAGGTCCCCGGGGGGACCACACCCAACACACAAGCCGTGCTTGAGGG CAGAAATGACGCTCGGACAGGCATGCCCCCCGGAATGCCAGGGGGCGCAATGTGCGTTCAAAGATTCGATGATTC ACGGAATTCTGCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATT GTTGAAAGTTTTGACAATTTTCTAGGTACTCAGACAGCCCTATCTTCTTCAGAGTTCAGGGGGTGCTCCGGCGGG CGCGGGCCCGGGGACAAACGTCCCCCGGCGACCAGGTAACCCCGGTGGGCCCGCCAAAGCAACAGGTATTATAAC GGCACGGGTGGGAGGTTGAGCCCAGAGGGCTCGCACTCGGTAATGATCCTTCCGCAGGTTCACCTACGGAAACCT TGTTA Talaromyces helicus var major strain MH480 Sequence ID: FJ430765.1 Identities: 453/459(99%) ATTTGGTGGTGACCAACCCCCGCAGGTCCCTCCCGAGCGAGTGACAAAGCCCCATGCGCTCGAGGACCCGACGGG CGTCGCCGCTGCCTTTGGGGCAGGTCCCCGGGGGGACCACACCCAACACACAAGCCGTGCTTGAGGGCAGAAATG ACGCTCGGACAGGCATGCCCCCCGGAATGCCAGGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATT CTGCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAG Hoàng Văn Năm Page72 VIỆN ĐẠI HỌC MỔ HÀ NỘIKHOA CÔNG NGHỆ SINH HỌC TTTTGACAATTTTCTAGGTACTCAGACAGCCCTATCTTCTTCAGAGTTCAGGGGGTGCTCCGGCGGGCGCGGGCC CGGGGACAAACGTCCCCCGGCGACCAGGTAACCCCGGTGGGCCCGCCAAAGCAACAGGTATTATAACGGCACGGG TGGGAGGTTGAGCCCAGAGGGCTCGCACTCGGTAATGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTA Talaromyces barcinensis Sequence ID: JN899349.1Identities: 502/513(98%) >LPH 242-1 9.5-4 ITS 5-ITS4 ITS 600 VT 1550 GTTGGGTGTCGGCTGGCGCCGGCCGGGCCTACAGAGCAGGTGACAAAGCCCCATACGCTCGAGGACCGGACGCGG TGCCGCCGCTGCCTTTCGGGCCCGTCCCCCGGGAGAGGGGGACGGGGGCCCAACACACAAGCCGTGCTTGAGGGC AGCAATGACGCTCGGACAGGCATGCCCCCCGGAATACCAGGGGGCGCAÃTGTGCGTTCAAAGACTCGATGATTCÃ CTGAATTCTGCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTG TTGAAÃGTTTTAACTGATTACGATAATCAACTCAGACTGCATACTTTCAGAACAGCGTTCATGTTGGGGTCTTCG GCGGGCGCGGGCCCGGGGGCGCAAGGCCTCCCCGGCGGCCGTCGAAACGGCGGGCCCGCCGAAGCAACAAGGTAC GATAGACACGGGTGGGAGGTTGGACCCAGAGGGCCCTCACTCGGTAATGATCCTTCCGCAGGTTCACCTACGGAA ACCTTGTTA Aspergillus fumigatus Sequence ID: KP131574.1 Identities: 534/534 (100%) >LPH 242-2 10.5-4 ITS 5-ITS4 ITS 600 VT 1551 ATGGAAGACCAACGCCCGTAGATCCTTCCCGAGCGGGTGACAGAGCCCCATACGCTCGAGGÃCCTAGGCGGGCGT CGCCACTGCCTTTCAGGCAGGTCCCCGGGGGGACCACACCCAACACACAAGCCGTGCTTGAGGGCAGAAATGACG CTCGGACAGGCATGCCCCCCGGAATGCCAGGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTG CAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTT TGATTATTATCAATCATGCTCAGACAGTCCATCTTCATGAGGGTTCAAGGGCGCTCTGGCGGGCACGGGCCCAGG GGCACAGGGGCTCCTCGGCGACCGGGAGAGCCCAGTGGGCCCGCCAAAGCAACAGGGTGTACAAGAGACAAGGGT GGGAGGTTCGGTCCACGCGGGACCGGCACTCAGTAATGATCCTTCCGCAGGTTCACCTACGGAACGGTTAGGTTT CACTTGGGGGGGTTTAGGCCCCAACAGTGTACTCGCCCCCCTGGTCTCCCAGGGAGCCCGACTATATCTTAAGCÃ GGACTCGACGTCCCACCCACTTCCACTTAGTCTGTGAACGTTCCCCATATCCCCCCTGAAACTACAGGGGACACT TAGGGGCTTCGATGCGGATTACCCATGGTCTCTGCCTGGAGCAGAACTATCCGAATCGTCTGTTACCGTCCCCCG TGGTGTGTCCACGGGCCCỊCpì^A^Ỹệ^l^C^G^A^Gppg^T^ỹpộT^Gp^ựpGGCGTCCCCGTCAATT Talaromyces solicola Sequence ID: KF114730.lidentities: 436/444(98%) >LPH 243 11.5-4 ITS 5-ITS4 ITS 600 VT 1552 GTTGCTGGATTATACTCTTGTACTTTACTTCCTGGGCGAACCAAAGAAAAAGATCCTGAGACCAGCGTAATATTC CTGCCTAGCAAGCCAGACAGAAAATCACACACATTTTAGGTGCTCACTGTAATAAAACAGCGATGCGACCCATTA CCACATAAACAAATGTTATGTGTGGGTTTGTGATGATACTGAAGCAGGCGTACTCTATAGAAAAACCATAGAGTG CAAGCTGCGTTCAAAGACTCGATGATTCACTGAATATGCAATTCACACTAGTTATCGCACTTTGCTACGTTCTTC ATCGATGCGAGAACCAAGAGATCCATTGTTAAAAGTTGTTTTTTATTAAACTTTATAATACTGAATTTCTAGGTT TATTATGAAGGGTGCTCCTGAAACCAGGAGTGGCATCGATCAAACCCCAGATAGGTCTACCCATGACCAGTCTGA GTCTCTCAGCCAAATTTTCACAGTGTAGAAGCAATCACTTACCCCAGAGGAAACCCTAAGAGGTAAGGCGCTTTA ACATAATTAATGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTAC Rhizopus oryzae Sequence ID: AB109754.1 Identities: 573/573(100%) >LPH 244 12.5-4 ITS 5-ITS4 ITS 600 VT 1553 Khơng có TT >LPH 245-1 13.5-4 ITS 5-ITS4 ITS 600 VT 1554 GATGGAAGACCAACGCCCGTAGATCCTTCCCGAGCGGGTGACAGAGCCCCATACGCTCGAGGACCTAGGCGGGCG TCGCCACTGCCTTTCAGGCAGGTCCCCGGGGGGACCACACCCAACACACAAGCCGTGCTTGAGGGCAGAAATGAC GCTCGGACAGGCATGCCCCCCGGAATGCCAGGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT GCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTT TTGATTATTATCAATCATGCTCAGACAGTCCATCTTCATGAGGGTTCAAGGGCGCTCTGGCGGGCACGGGCCCAG GGGCACAGGGGCTCCTCGGCGACCGGGAGAGCCCAGTGGGCCCGCCAAAGCAACAGGGTGTACAAGAGACAAGGG Hoàng Văn Năm Page73 VIỆN ĐẠI HỌC MỔ HÀ NỘIKHOA CÔNG NGHỆ SINH HỌC TGGGAGGTTCGGTCCACGCGGGACCGGCACTCAGTAATGATCCTTCCGCAGGTTCACCTACGGAACGGTTAGGTT TCACTTGGGGGGGTTTAGGCCCCAACAGTGTACTCGCCCCCCTGGTCTCCCAGGGAGCCCGACTATATCTTAAGC AGGACTCGACGTCCCACCCACTTCCACTTAGTCTGTGAACGTTCCCCATATCCCCCCTGAAACTACAGGGGACAC TTAGGGGCTTCGATGCGGATTACCCATGGTCTCTGCCTGGAGCAGAACTATCCGAATCGTCTGTTACCGTCCCCC GTGGTGTGTCCACGGGCCCCTGCACGGTTTCCCGCGGCAGGTTGGTGCGATCGGCTTTAGGGCGTCCCCGTCAAT TCAAAAGTGTCGCCCAGCCCGGGAGGGGCTGGACTTGCAACGGCACCATGTTCTT Talaromyces solicola Sequence ID: KF114730.lldentỉties: 436/444 (98%) Talaromyces albobiverticiỉtius Sequence ID: KF114738.1 Idem ities: 435/445 (98%) >LPH 245-2 14.5-4 ITS 5-ITS4 ITS 600 VT 1555 GATGGAAGACCAACGCCCGTAGATCCTTCCCGAGCGGGTGACAGAGCCCCATACGCTCGAGGACCTAGGCGGGCG TCGCCACTGCCTTTCAGGCAGGTCCCCGGGGGGACCACACCCAACACACAAGCCGTGCTTGAGGGCAGAAATGAC GCTCGGACAGGCATGCCCCCCGGAATGCCAGGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCT GCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTT TTGATTATTATCAATCATGCTCAGACAGTCCATCTTCATGAGGGTTCAAGGGCGCTCTGGCGGGCACGGGCCCAG GGGCACAGGGGCTCCTCGGCGACCGGGAGAGCCCAGTGGGCCCGCCAAAGCAACAGGGTGTACAAGAGACAAGGG TGGGAGGTTCGGTCCACGCGGGACCGGCACTCAGTAATGATCCTTCCGCAGGTTCACCTACGGAACGGTTAGGTT TCACTTGGGGGGGTTTAGGCCCCAACAGTGTACTCGCCCCCCTGGTCTCCCAGGGAGCCCGACTATATCTTAAGC AGGACTCGACGTCCCACCCACTTCCACTTAGTCTGTGAACGTTCCCCATATCCCCCCTGAAACTACAGGGGACAC TTAGGGGCTTCGATGCGGATTACCCATGGTCTCTGCCTGGAGCAGAACTATCCGAATCGTCTGTTACCGTCCCCC GTGGTGTGTCCACGGGCCCCTGCACGGTTTCCCGCGGCAGGTTGGTGCGATCGGCTTTAGGGCGTCCCCGTCAAT TCAAAAGTGTCGCCCAGCCCGGGAGGGGCTGGACTTGCAACGGCACCATGTTCTTTAGAGTC Talaromyces solicola Sequence ID: KF114730.lidentities: 436/444 (98%) Talaromyces albobiverticillius Sequence ID: KF114738.lidentities: 435/445(98%) >LPH 256 Tihj-4 5! VlTS 5-ITS4 ^TS 600 v^(1556 ttgatttgcgttcggcaagcgccggccgggcctacagagcgggtgacaaagccccatacgctcgaggatcggacg CGGTGCCGCCGCTGCCTTTGGGGCCCGTCCCCCCCGGAGAGGGGACGACGACCCAACACACAAGCCGTGCTTGAT GGGCAGCAATGACGCTCGGACAGGCATGCCCCCCGGAATACCAGGGGGCGCAATGTGCGTTCAAAGACTCGATGA TTCACGGAATTCTGCAATTCACACTAGTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCC ATTGTTGAAAGTTTTAACTGATTGCGATACAATCAACTCAGACTTCACTAGATCAGACAGAGTTCGTGGTGTCTC CGGCGGGCGCGGGCCCGGGGCTGAGAGCCCCCGGCGGCCATGAATGGCGGGCCCGCCGAAGCAACTAAGGTACAG TAAACACGGGTGGGAGGTTGGGCTCGCTAGGAACCCTACACTCGGTAATGATCCTTCCGCAGGTTCACCTACGGA AACCTTGTTAC Aspergillus flavus Sequence ID: KR611590.lidentities: 536/536 (100%) >LPH 257-1 16.5-4 ITS 5-ITS4 ITS 600 VT 1557 GACCAAGGCCTTCTAACAAAATCCTGCATCAGTCAGCTCACACAAGAGGACCTTAGAGTAGCTGCAGCAAATTAG AAATAATCCGCCGAAACGGACTAAATAGATATGCTTTTAGCTCAGTCTGTTCTTGTCACAGCTGCACCTGCATTT CAAGCAACCCGCCGCATTTGCTAGGCGCATTTGCCGTTGGGTTTGCTCAAATCCATCCAAAÃTTCAAAAAATAGÃ ATTTTGGTTGAGGTGTTTACTGATACTCAAACAAGCATGCTCTTCGGAATACCAAAGAGCGCAATATGCGTTCAA AGATTCGATGATTCACTGAATTCTGCAATTCACATTACGTATCGCATTTCGCTGCGTTCTTCATCGATGCGAGAG CCAAGAGATCCGTTGTTGAAAGTTATGTTTTAGAATTTAGTCAATTCTCTAACATTTTCAGACAATCGTATTATA GAAAAGTGTATATTAAAATTGAAAAGAAGAAAGCAGAAAAAAAGCCTAAAAACGATTTGCATCGTTAGACTTTTT TTACACACTCTCTCTTTTTACATTGCACACAAGGTGGGTATGGATTTTTTGAAGAGTGCCATAAACACTCTTATT GTGAATGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTT Mortierella wolfii Sequence ID: HQ630304.1Identities: 611/611 (100%) >LPH 257-2 17.5-4 ITS 5-ITS4 ITS 600 VT 1558 TGTTTTACGGACGTGGACGCGCCGCGCTCCCGGTGCGAGTTGTGCAAACTACTGCGCAGGAGAGGCTGCGGCGAG ACCGCCACTGTATTTCGGGGCCGGCACCCGTGTGAGGGGTCCCGATCCCCAACGCCGATCCCCCGGAGGGGTTCG AGGGTTGAAATGACGCTCGGACAGGCATGCCCGCCAGAATACTGGCGGGCGCAATGTGCGTTCAAAGATTCGATG ATTCACTGAATTCTGCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCAGAACCAAGAGATC Hoàng Văn Năm Page74 VIỆN ĐẠI HỌC MỔ HÀ NỘIKHOA CÔNG NGHỆ SINH HỌC CGTTGTTGAAAGTTTTGATTCATTTTGAATTTTTGCTCAGAGCTGTAAGAAATACGTCCGCGAGGGGACTÃCAGA AAGAGTTTGGTTGGTTCCTCCGGCGGGCGCCTGGTTCCGGGGCTGCGACGCACCCGGGGCGTGACCCCGCCGAGG CAACAGTTTGGTAACGTTCACATTGGGTTTGGGAGTTGTAAACTCGGTAATGATCCCTCCGCTGGTTCACCAACG GAGACCTTGTTAC Trichoderma asperellum Sequence ID: KT824429.lldentities: 538/538 (100%) >LPH 259 19.5-4 ITS 5-ITS4 ITS 600 VT 1559 GATGGTGTTCGCCGGCGGGCGCCGGCCGGGCCTACAGAGCGGGTGACGAAGCCCCATACGCTCGAGGACCGGACG CGGTGCCGCCGCTGCCTTTCGGGCCCGCCCCCCGGÃAGCGGGGGGCGAGAGCCCAACACACÃAGCCGTGCTTGAG GGCAGCAATGACGCTCGGACAGGCATGCCCCCCGGAATACCAGGGGGCGCAATGTGCGTTCAAAGACTCGATGAT TCACTGAATTCTGCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCG TTGTTGAAAGTTTTAACTGATTTAGTCAAGTACTCAGACTGCAATCTTCAGACAAGAGTTCGTTTGTGTGTCTTC GGCGGGCGCGGGCCCGGGGGCGGATGCCCCCCGGCGGCCGTGAGGCGGGCCCGCCGAAGCAACAAGGTACGATAA ACACGGGTGGGAGGTTGGACCCAGAGGGCCCTCACTCGGTAATGATCCTTCCGCAGGTTCACCTACGGAAACCTT GTTACG Penicillium oxalicum Sequence ID: KP132493.1Identities: 531/531 (100%) >LPH 260 20.5-4 ITS 5-ITS4 ITS 600 VT 1560 GATTGGGGTCGCCGGCGGGCGCCGGCCGGGCCTACAGAGCGGGTGACGAAGCCCCATACGCTCGAGGACCGGACG CGGTGCCGCCGCTGCCTTTCGGGCCCGCCCCCCGGGAGCCGGGGGGCGAAGCCCAACACACAAGCCGTGCTTGAG GGCAGCAATGACGCTCGGACAGGCATGCCCCCCGGAATACCAGGGGGCGCAATGTGCGTTCAAAGACTCGATGAT TCACTGAATTCTGCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCG TTGTTGAAAGTTTTAACTGATTTAGCTAATCTGCTCAGACTGCAATCTTCAGACAGAGTTCATTGGTGTCTTCGG CGGGCGCGGGCCCGGGGGCGAGTGCCCCCCGGCGGCCGTGAGGCGGGCCCGCCGAAGCAACAAGGTAGAATAAAC ACGGGTGGGAGGTTGGACCCAGAGGGCCCTCACTCGGTAATGATCCTTCCGCAGGTTCACCTACGGAAACCTTGT TAC Penicillium simplicissimum sequence m H?l%N0i >LPH 261 21.5-4 ITS 5-ITS4 ITS 600 VT 1561 TGTTTTACGGACGTGGACGCGCCGCGCTCCCGGTGCGAGTTGTGCAAACTACTGCGCAGGAGAGGCTGCGGCGAG ACCGCCACTGTATTTCGGGGCCGGCACCCGTGTGAGGGGTCCCGATCCCCAACGCCGATCCCCCGGAGGGGTTCG AGGGTTGAAATGACGCTCGGACAGGCATGCCCGCCAGAATACTGGCGGGCGCAATGTGCGTTCAAAGATTCGATG ATTCACTGAATTCTGCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCAGAACCAAGAGATC CGTTGTTGAAAGTTTTGATTCATTTTGAATTTTTGCTCAGAGCTGTAAGAAATACGTCCGCGAGGGGACTACAGA AAGAGTTTGGTTGGTTCCTCCGGCGGGCGCCTGGTTCCGGGGCTGCGACGCACCCGGGGCGTGACCCCGCCGAGG CAACAGTTTGGTAACGTTCACATTGGGTTTGGGAGTTGTAAACTCGGTÃATGATCCCTCCGCTGGTTCACCAACG GAGACCTTGTTAC Trichoderma asperellum Sequence ID: KM456217.lidentities: 538/538 (100%) >LPH 262 22.5-4 ITS 5-ITS4 ITS 600 VT 1562 GTTGCAAATAAATGCGTCGGCGGGCGCCGGCCGGGCCTACGGAGCGGAAGACGAAGCCCCATACGCTCGAGGACC GGACGCGGTGCCGCCGCTGCCTTTCGGGCCCGTCCCCCGGGAGCCGGGGGACGAGGGCCCAACACACAAGCCGGG CTTGAGGGCAGCAATGACGCTCGGACAGGCATGCCCCCCGGAATACCAGGGGGCGCAATGTGCGTTCAAAGACTC GATGATTCACTGAATTCTGCAATTCACATTAGTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGA GATCCATTGTTGAAAGTTTTAACTGATTGCAAAGAATCACACTCAGACTGCAAGCTTTCAGAACAGGGTTCATGT TGGGGTCTCCGGCGGGCACGGGCCCGGGGGCGAGTCGCCCCCCGGCGGCCAGCAACGCTGGCGGGCCCGCCGAAG CAACAAGGTACAATAGTCACGGGTGGGAGGTTGGGCCATAAAGACCCGCACTCGGTAATGATCCTTCCGCAGGTT CACCTACGGAAACCTTGTTAC Aspergillus terreus Sequence ID: KR610363.lidentities: 546/546 (100%) >LPH 263 23.5-4 ITS 5-ITS4 ITS 600 VT 1563 TTTGGTGGTGACCAACCCCCGCAGGTCCCTCCCGAGCGAGTGACAAAGCCCCATGCGCTCGAGGACCCGACGGGC GTCGCCGCTGCCTTTGGGGCAGGTCCCCGGGGGGACCACACCCAACACACAAGCCGTGCTTGAGGGCAGAAATGA CGCTCGGACAGGCATGCCCCCCGGAATGCCAGGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTC Hoàng Văn Năm Page75 VIỆN ĐẠI HỌC MỔ HÀ NỘIKHOA CÔNG NGHỆ SINH HỌC TGCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGT TTTGACAATTTTCTAGGTACTCAGACAGCCCTATCTTCTTCAGAGTTCAGGGGGTGCTTCGGCGGGCGCGGGCCC GGGGACAGGCGTCCCCAAGCGACCAGGTGACCCCAGTGGGCCCGCCAAAGCAACAGGTATTATAACGGCACGGGT GGGAGGTTGAGCCCAGAGGGCTCGCACTCGGTAATGATCCTTCCGCAGGTTCACCTACGGAACGGCTAGAGTTCA CAATGGGAAGTTTCAACAGAGTTTTGACTGGGGGACTCAACTAGAAAGCTGCGTCCTGAGGCAAGCGCACATCTC AACACCAGGAAGGGTACATATAAATACCACGTGACATATGCAATCCTCGAAACAACCCCTAGACAGCTCCCCCTC TGTAAAAATCCCAAACAGTGTACTTGTCTCTCCAGTTTCCTAGAGAGCCCGACTATATCTTAAGCAGGACCCCGG AACAGGGTGCCCACCCACTTCCACTTAGTCTGTGAACGTTCCCCATATCCCGGAGGACGTAGGGGCTTCGCTGCG GATTGCCCATCATCTCTGCCTGGGGCAGAACTATCCAAGTCGTCTATTACCTTCCCCCATGATGTGTTCAT Talaromyces helicus Sequence ID: KM066176.lldentities: 446/448 (99%) >LPH 263-1X 24.5-4 ITS 5-ITS4 ITS 600 VT 1564 ATCGTGGGTGGCCAACGCCCGCAGGCCCCTCCCGAGCGGGTGACAAAGCCCCATACGCTCGAGGACCTAGACGGG ACGTCGCCGCTGCCTTTCGGGCAGGTCCCCCCAACACCGGGGGGACCACACCCAACACACAAGCCGCGCTTGAGG GCAGAAATGACGCTCGGACAGGCATGCCCCCCGGAATGCCAGGGGGCGCAATGTGCGTTCAAAGATTCGATGATT CACGGAATTCTGCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCAT TGTTGAAAGTTTTGATAATTCAAAATCACACTCAGACAGCCCATCTTCATTAGGGTTCACAGAGCGCTCTGGCGG GCGCAGGCCCGGGGGCGCAGTGCCCCCCGGCGACCGGGGCGACCCGGTGGGCCCGCCAAAGCAACAGGGTATTCA AGAGACACGGGTGGGAGGTTGGACCCGCGAGGGGTCCGCACTCAGTAATGATCCTTCCGCAGGTTCACCTACGGA AACCTTGTTACG Talaromyces atroroseus Sequence ID: NR_137815.1 Identities: 453/453 (100%) >LPH 263-2T 25.5-4 ITS 5-ITS4 ITS 600 VT 1565 GGTGGTGACCÃACCCCCGCAGGTCCCTCCCGAGCGAGTGACAAAGCCCCATGCGCTCGAGGACCCGACGGGCGTC GCCGCTGCCTTTGGGGCAGGTCCCCGGGGGGACCACACCCAACACACAAGCCGTGCTTGAGGGCAGAAATGACGC TCGGACAGGCATGCCCCCCGGAATGCCAGGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGC AATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTGTTGAAAGTTTT GACAATTTTCTAGGTAC'IICAGACAGeCC-IWrm'ZC'rECAGAGTTCAGGGGGTG.C'MCGGCGGGCGCGGGCCCGGG GACAGGCGTCCCCAAGCGACCAGGTGACCcd^GGG^HcGCCAAAGCAACAl&TATTATAACGGCACGGGTGGG AGGTTGAGCCCAGAGGGCTCGCACTCGGTAATGATCCTTCCGCAGGTTCACCTACGGAACGGCTAGAGTTCACAA TGGGAAGTTTCAACAGAGTTTTGACTGGGGGACTCAACTAGAAAGCTGCGTCCTGAGGCAAGCGCACATCTCAAC ACCAGGAAGGGTACATATAAATACCACGTGACATATGCAATCCTCGAAACAACCCCTAGACAGCTCCCCCTCTGT AAAAATCCCAAACAGTGTACTTGTCTCTCCAGTTTCCTAGAGAGCCCGACTATATCTTAAGCAGGACCCCGGAAC AGGGTGCCCACCCACTTCCACTTAGTCTGTGAACGTTCCCCATATCCCGGAGGACGTAGGGGCTTCGCTGCGGAT TGCCCATCATCTCTGCCTGGGGCAGAACTATCCAAGTCGTCTATTACCTTCCCCCATGATGTGTTCAT Talaromyces barcinensis Sequence ID: NR-137078 Identities: 504/509 (99%) >LPH 264 26.5-4 ITS 5-ITS4 ITS 600 VT 1566 GGAAAACGTCGGCAGGCGCCGGCCAATCCTACAGAGCATGTGACAAAGCCCCATACGCTCGAGGATCGGACGCGG TGCCGCCGCTGCCTTTCGGGCCCGTCCCCCCGGAGAGGGGGACGGCGACCCAACACACAAGCCGGGCTTGAGGGC AGCAATGACGCTCGGACAGGCATGCCCCCCGGAATACCAGGGGGCGCAATGTGCGTTCAAAGACTCGATGATTCA CTGAATTCTGCAATTCACATTAGTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTG TTGAAAGTTTTAACTGATTGCATTCAATCAACTCAGACTGCACGCTTTCAGACAGTGTTCGTGTTGGGGTCTCCG GCGGGCACGGGCCCGGGGGGCAGAGGCGCCCCCCCGGCGGCCGACAAGCGGCGGGCCCGCCGAAGCAACAGGGTA CAATAGACACGGATGGGAGGTTGGGCCCAAAGGACCCGCACTCGGTAATGATCCTTCCGCAGGTTCACCTACGGA AACCTTGTTA Aspergillus niger Sequence ID: KP724986.1Identities: 535/535 (100%) >LPH 265 27.5-4 ITS 5-ITS4 ITS 600 VT 1567 AACGGCACGCCGCCGTCCACACCCATCGAGCGACTGAACAAAGTCTCGCAACGCTCGGGGACGGACGGCCGGGCC GGACGGCGTTTGAGGCGCGCGGCGGCATCGCCGCGGCGCCCAATACCCAGCCGGGCTGGAGTGGTGCAATGACGC TCGAACAGGCATGCCCCCCGGAATACCAAGGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACTGAATTCTGC AATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCAGAACCAAGAGATCCGTTGTTGAAAGTTTT GTTTGATTTCGCTTTACGCTCAGACGCTCAGCAAGACAGTGTTGGGAAGGTCCCGCGGGGGCCCCGGCGCGCGGC Hoàng Văn Năm Page76 VIỆN ĐẠI HỌC MỔ HÀ NỘIKHOA CÔNG NGHỆ SINH HỌC CGGGTCACCCCCCAGGCATAAGGGGTTGAAGGTTTGGGGGTCGGAGGTCGGGCCGGAACCCTCACTCGGTÃATGA TCCCTCCGCAGGTTCACCTACGGAGACCTTGTTAC Acidomyces acidothermus Sequence ID: JQ904606.lldentities: 484/485 (99%) >LPH 266 28.5-4 ITS 5-ITS4 ITS 600 VT 1568 CTGAGAAGATTGGGGGTCGAGGCAAGCCCCGGCCGGGCCCATAGAGCGGGTGACAGAGCCCCATACGCTCGAGGA CCGGACGGTGCCGCCGTTTCTCTCGAGGCCCGCCCCCGGGGGGGCGCGGCCCAACAACCAGCGGGGCTGGAGGGG AGAAATGACGCTCGGACAGGCATGCCCCCCGGAATACCAGGGGGCGCAATGTGCGTTCAAAGACTCGATGATTCA CTGAATTCTGCAATTCACATTAGTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCATTG TTGAAAGTTTTGACTGATTGGTATCAATCGACTCAGACTGCACGCTTTCAGACAGTGTTCCATTGGGGTCTCCGG CGGGCGCGGTCCCGGGGGCAGGCCCCGGGCCGCCCGAAGGCGGGCCCGCCGAAGCAACAGGGTACGGTAAGCACG GGTGGGAGGTTGGGCCCCGAAGGACCCAGCACTCGGTAATGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTT A Aspergillus aculeatus Sequence ID: JX501384.1Identities: 517/517 (100%) >LPH 267 29.5-4 ITS 5-ITS4 ITS 600 VT 1569 TCACCTGGTAAGAAGAGGTTGCCAGGCTGGCGCCGGCCGGTCCTGCAGAGCGGGTGACAGAGCCCCATACGCTCG AGGATCGGACGCGGTACCGCCGCTGCCTTTCGGGCCCGTCCCCCCCCGGAGCGGGGGGACGGCGACCCAACACAC AAGCCGGGCTTGAGGGTAGCAATGACGCTCGGACAGGCATGCCCCCCGGAATACCAGGGGGCGCAATGTGCGTTC AAAGACTCGATGATTCACTGAATTCTGCAATTCACACTACTTATCGCAGTTCGCTGCGTTCTTCATCGATGCCGG AACCAAGAGATCCGTTGTTGAAAGTTTTGACTGATTTTAGAAATCCACTCAGACGGCAGTCTTCAGACAGCGTTC GTGTGGTCCCCGGCGGGCGCTCGCACGGGGGCCGAAGCCCCATGGCGGCCCCCAAGGTTGGGGGCGGGCCCGCCG AAGCAACGTGGTACGGAGACACGGATGGGAGGTTGGCACCCCGAAGGGGCCGCACTCGGTAATGATCCTTCCGCA GGTTCACCTACGGAAACCTTGTTA Phialosimplex caninus Sequence ID: GQ169313.lidentities:491/537 (91%) GACCTTC^AGGCTATG^^^aWo^WSM^W^GCTCGAGGACCGGA CGCGACGCCGCCGCTGCCTTTCAGGCCCGTCCCCTCCAAACGAGGGGACGGCGGCCCAACACACAAGCCGTGCTT GAGGGTTCGCAATGACGCTCGGACAGGCATGCCCCCCGGAATACCAGAGGGCGCAATGTGCGTTCAAAGATTCGA TGATTCACGGAATTCTGCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGA TCCATTGTTGAAAGTTTTGAACGATTGCATTTCGTCACTCAGACAATCCTCGCATTCACAAAAGCGTTCACAGTG AGTGCCTCTGGCGGGCACGGGCCCCGGGGGCACACCGCGCCCCGGGACGACCCCCCCGGCGGAACACCGGAGGAG TGGGCCCGCCAAAGCAACACTAGGTGTGGTAGACACGGGTGGGAGATTGGGACCCGACTGGGTTCCCGCACTCGG TAATGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTA Thermomyces lanuginosus Sequence ID: NR—121309.lidentities : 565/565 (100%) >LPHT 221 221 5-4 ITS 5-ITS4 ITS 600 VT 1571 TGCTTTCCGTTTAACGGCTAGGACCTAGAGCATGCCTTAAGCGAGGTAGTTGCTACTATGCTTAGGGTGATAGTG GGCACCGCCATTGGTTTTTAGGGCCTGCGGGTAGCTGTAGGTCCCTAACACAAGCCCGGGGCTTGATGGTCGAAA TGACGCTCGGACAGGTATACCCGCCGGAATGCCGGCGGGCGTAATGTGCGTTCAAAGATTCGATGATTTACGGAA TTCTGCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTTATCGATGCTAGAACCAAGAGATCCGTTGTTGAA AGTTTTGACTTATTCAGTATAGAAGACTTAGAGAGGCCTTGTGTATACGAGAATGGTTTGGAGCCTCCGGCGGGC GCCTCTGCGAGGGGGGCTAGGGGGCGCGCTGGCGGGCCCGTAGGGGCCCTGGCGCTGCCCGCCGAAGCAACGGTT AGGTAAGGTCCACAGTGGTTTGGGAGTCTTGCAACTCTGTAATGATTCCTCCGCTAGTTCACCAACGGAGACCTT GT Thielavia terrestris Sequence ID: CP003011.1 Identities: 488/527 (93%) >LPHT 222 222 5-4 ITS 5-ITS4 ITS 600 VT 1572 GCTTTCCGTTTAACGGCTGGGACCTGGAGTATACCCTGAGCGAGGTGGTTGCTACTACGCTTGGGGTGACAGTGG GTACCGCCACTGGTTTTCGGGGCCTGTGGGTAGTCGTAGGTCCCTAATACAAGCTTGGGGCTTGATGGTCGAAAT Sordariales Sequence ID: KƯ365891.1Identities: 134/150 (89%) Thielavia sp Hoàng Văn Năm Page77 VIỆN ĐẠI HỌC MỚ HÀ NỘIKHOA CÔNG NGHỆ SINH HỌC Sequence ID: KJ813011.lidentities: 134/150 (89%) >LPHT 223 223 5-4 ITS 5-ITS4 ITS 600 VT 1573 ACCTTCAGAGGCTATGGCCCCGGCCGGACCCCTCCTTGAGCGCGTGACAGAGCCCCATACGCTCGAGGACCGGAC GCGACGCCGCCGCTGCCTTTCAGGCCCGTCCCCTCCAAACGAGGGGACGGCGGCCCAACACACAAGCCGTGCTTG AGGGTTCGCAATGACGCTCGGACAGGCATGCCCCCCGGAATACCAGAGGGCGCAATGTGCGTTCAAAGATTCGAT GATTCACGGAATTCTGCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGAT CCATTGTTGAAAGTTTTGAACGATTGCATTTCGTCACTCAGACAATCCTCGCATTCACAAAAGCGTTCACAGTGA GTGCCTCTGGCGGGCACGGGCCCCGGGGGCACACCGCGCCCCGGGACGACCCCCCCGGCGGAACACCGGAGGAGT GGGCCCGCCAAAGCAACACTAGGTGTGGTAGACACGGGTGGGAGATTGGGACCCGACTGGGTTCCCGCACTCGGT AATGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTA Thermomyces lanuginosus Sequence ID: NR-121309.1 Identities: 564/564 (100%) >LPHT 225 225 5-4 ITS 5-ITS4 ITS 600 VT 1574 CTTTCCCGTTTAACGGCCAGGACCTGGAGCACGCCCTGAGCAAGTGGTTGCTACTATGCTCGGGGTGACAGCGGG CTCCGCCACTGGTTTTCGGGCCTATAAGTAGCCGTAGGTCCCTAACATAAGCCTAGGGGCTTAATGGTCGAAATG ACGCTCGGACAGGCATGCCCGCCAGAATGCTAGCGGGCGCAATGTATATTTAAAGATTCGATGATTCACGGAATT CTGCAATTCACATTACTTATCGCATTTCGCTACGTTCTTCATCGATGCTAGAAC Thielavia sp Sequence ID: KJ813011.lidentities: 253/281 (90%) >LPHT 225 225 5-4 ITS5-ITS4 ITS5 600 VT 1660 TTGATTGGGGTCGCCGGCGGGCGCCGGCCGGGCCTACAGAGCGGGTGACGAAGCCCCATACGCTCGAGGACCGGA CGCGGTGCCGCCGCTGCCTTTCGGGCCCGCCCCCCGGGAGCCGGGGGGCGAAGCCCAACACACAAGCCGTGCTTG AGGGCAGCAATGACGCTCGGACAGGCATGCCCCCCGGAATACCAGGGGGCGCAATGTGCGTTCAAAGACTCGATG ATTCACTGAATTCTGCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGATC CGTTGTTGAAAGTTTTAACTGATTTAGCTAATCTGCTCAGACTGCAATCTTCAGACAGAGTTCATTGGTGTCTTC GGCGGGCGCGGGCCCGGGGGCGAGTGCCCCCCGGCGGCCGTGAGGCGGGCCCGCCGAAGCAACAAGGTAGAATAA ACACGGGTGGGAGGTTGt^AỊ^A^Iệ^CỤCỊlg^Cg^T^ộgAl^gTỊỊgp^íỰ^CACCTACGGAAACCTT ACTGTGGACCTTACTTAACCGTTGCTTCGGCGGGCGGCGCTAGGGCCCCTATAGGCCCGCTAGCGCGCCCCCCGG CCCCCCTCGCGGGGGCGCCCGCCGGAGGCTCTAAACTATTCTCGTATATACAAGGCCTCTCTGAGTCTTCTATAC TGAATGAGTCAAAACTTTCAACAACGGATCTCTTGGTTCTAGCATCGATGAAGAACGTAGCGAAATGCGATAAGT AATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAATATATATTGCGCCCGCTAGCATTCTGGCGGGTAT GCCTGTCCGAGCGTCATTTCGACTATCAAGCCCTAGGCTTGTGTTAGGGACCTGCGGCTACTCATAGGCTTCGAA AACCAGTGGCGGTGCCCGCTGTTACCCCGAGCATAGTAGCAACCACCTTGCTCAGGGCGTGCTCCAGGTCCTGGC CGTTAAACGGAAAGCAACCCCCTAAAGGTTGACCTCAGATTAGGTAGGAGGACCCACTAAACTTAAGCATATC Thielavia sp Sequence ID: KJ813011.1 Identities: 481/523(92%) >LPHT 227 227 5-4 ITS 5-ITS4 ITS 600 VT 1575 GTCTGACGGGTAGGCGGGCACCGGCCGGCACCTCCAGAGCGGGTGACAGAGCCCCATACGCTCAGGGACCGGAAA CGCGGCACCGCCGCTGCCTTTCGGGCCCGTCCCTCCCCGCGGGAAGGGACGGCGGCCCAACACACAAGCCGTGCT TGAGGGGCAGAAATGACGCTCGGACAGGCATGCCCCCCGGAATGCCAGAGGGCGCAATGTGCGTTCAAAGATTCG ATGATTCACGGAATTCTGCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAG ATCCGTTGTTGAAAGTTTTGACGATTGTGTTTTCCACTCAGACCGCAACCTTTGTTCAAGACAGCGTTCACGAGG GTCTCCGGCGGGCACGGGCCCGGAGGCACGGGAGATGGCCCCCCGGCGGCCAGGGACAGGAGCCCCCCTGGCGGG CCCGCCGAAGCAACACGGTACGGTAGACACGGGTGGGAGGTCGGGCCCGCAAAGGGGACCCTCACTCGGTAATGA TCCTTCCGCAGGTTCACCTACGGAAACCTTGTTAC Rasamsonia emersonii Sequence ID: JF417478.lldentities: 559/559 (100%) >LPHT 228 228 5-4 ITS 5-ITS4 ITS 600 VT 1576 AGTTGAATTTATTTGTGGTTGATTGACCTTTATACTTGATCATCTCGAAGCACACTGGTAAAAAATTAGATACCA ATGCAAGCCCTCAAGGAAATATCAAGGGTCAACAACATACAAATTGTTCGGGTAGCTTGAACGGATGAAAATCCA TCAGCACCGCAAATCCAAAGATTTATCAAAAAATAAACCTTTAGGGGTTAATAAAGATACTGAACTAGACGTACC CAATGGATGAACCAAAGGGTGCAAGGTGCGTTCGAGAATTCGATGATTCGCAAAGGCTGCAGATCGCATTACTTT Hoàng Văn Năm Page78 VIỆN ĐẠI HỌC MỔ HÀ NỘIKHOA CÔNG NGHỆ SINH HỌC TCGCAATTTGCTACGCTCTTCATCGATGCGAGAACCAAGTGATCCATTGCTTAAAGTTGTTTTGAAATTTTATCG TCTTGAATCATTTCTGATCCAAAACTTTCAATTTGTTAAACAACAGTCAATCAAATATGTAAAATAAAAGGTCGA GAGATCTGGAGCAATCCCTCAGAACATTTCACAAAAAGGAGAGAATCGCGGCTCACCÃATAGAGGTCACCACGAT TTCCACAACTTTTTAATGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTA Rhizomucor pusillus Sequence ID: JN206313.lldentities: 540/540 (100%) >LPHT 230 230 5-4 ITS 5-ITS4 ITS 600 VT 1577 (xấu) AGCTAGGACCTAGAGCACGCCCTAAGCAAGGTGGTTGCTACTATACTCAGGGTAATAGTGGGCACCGCTACTGGT TTTCAGGGCCTATAGGTAGCTACAGGTCCCCAACACAAGCCTAGGGCTTGATAGTCGAAATGACGCTTGGACAGG TATACCTACTAGAATACCGGTAGGCGCAATGTACGTTCAAAGATTCGATGATTCATAGAATTCTACAATTCATAT TACTTATCGTATTTTGCTACGTTCTTTATCGATACTAGAACTAAGAGATCTATTGTTGAAAGTTTTAACTTATTT AGTATAGAAGACTCAGAGAGGCCTTGTATATACGAGAATAGTTTAGAGCCTCTAGTAGGCGCCTCTGCGAGGGGG GCCAGGGGGCGTGCTAGCAGGCCTATAGGGGCCCTAGCGCCGTCCGCCAAAGCAACGGTTAGGTAAGGTCTATAA TAGTTTAGGAGTCTTACAACTCTATAATAATCCCTCTGCTAGTTCATTAACGGAGACCTTGT Thielavia ovispora Sequence ID: AJ271593.lldentities: 431/491 (88%) >LPHT 230 230 5-4 ITS5-ITS4 ITS5 600 VT 1662 GAGTTGTAAGACTCCTAAACTATTATAGACCTTACCTAACCGTTGCTTTGGCGGACGGCGCTAGGGCCCCTATAG GCCTGCTAGCACGCCCCCTGGCCCCCCTCGCAGAGGCGCCTACTAGAGGCTCTAAACTATTCTCGTATATACAAG GCCTCTCTGAGTCTTCTATACTAAATAAGTTAAAACTTTCAACAATAGATCTCTTAGTTCTAGTATCGATAAAGA ACGTAGCAAAATACGATAAGTAATATGAATTGTAGAATTCTATGAATCATCGAATCTTTGAACGTACATTGCGCC TACTGGTATTCTAGTAAGTATACCTGTCCAAGCGTCATTTCGACTATTAAGCCCTAGGCTTGTGTTGGGGACCTG TAGCTACCTATAGGCCCTGAAAACCAGTAGCGGTGCCTACTATTACCCCGAGTATAGTAGCAACCACCTTGCTTA GGGCGTGCTCTAGGTCCTAGCTATTAAACAGAÃAGTAACCCTCCAÃAGGTTGACCTCGGATTAGATAGGAGGACC CACTAAACTTAAGCATATCAATAA Thielavia ovispora Sequence ID: AJ271593.1 Identities:TRV5^iệh'%Viện Đại học Mở Hà Nội >LPHT 231 231 5-4 ITS 5-ITS4 ITS 600 VT 1578 TAGTGCTAAAGACGCAAAGGTCGCTTTACGGCCAGCACCCGCAGCACTCCCAAAGCGAGGTTGTAAGCTACTACG CTCGGTGTGTCTTGCGAGCCCGCCACTGCATTTCAGGGCCTGCGGCAGCCGCAGGTCCCCAACACAAGCCCAGGG CTTGATGGTTGAAATGACGCTCGAACAGGCATGCCCGCCGGAATACCGGCGGGCGCAATGTGCGTTCAAAGATTC GATGATTCACTGAATTCTGCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCAGAACCAAGA GATCCGTTGTTGAAAGTTTTGACTTATTCAGTACATAAACTCAGAGAGGCCATGTGCTGCAAAGGGTTTGGGGTA TTCCTCCGGCGGGAGCCTCCCCCCACGCCGCGGGGGCGGGGGATCGGCCGAGGGGCGCCGAGGCGCCTCCCGCCG AAGCAACAGAAGGGTCGGTTCACAATGGTTTGGTGAGTTGTGTAACTCTGTAATGATCCCTCCGCTGGTTCACCÃ ACGGAGACCTTGTTA Scytalidium thermophilum Sequence ID: FJ548831.1 Identities: 540/540 (100%) >LPHT 232 232 5-4 ITS 5-ITS4 ITS 600 VT 1579 CACCTTTGGGGGGTTGCTTTCTGTTTAATGGCTAGGACCTGGAGTACGCCCTAAGTAAGGTGGTTGCTACTACGC TTAGGGTGATAGTGGGCATCGCCACTGGTTTTCGGGGCCTATAGATAGCCGTAGGTCCCTAATACAAGCCTAGGG CTTGATAGTCGAAATGACGCTCAGACAGATATATCTACTAGAATATTGGTAGGCGCAATATACGTTTAAAGATTC GATGATTCATAGAATTCTACAATTCACATTACTTATTGTATTTTGCTATATTCTTTATCGATGCTAGAACCAAGA GATCCATTGTTAAAAGTTTTGACTCATTTAGTATAGAAGACTCAGAGAGGCCTTGTATATACAAGAATAGTTTAG AGCCTCCGGTGGGCGCCCCCGCGAGGGGGGCCGGGGGGCGCGCTAGCGGGCCTGTAGGGGCCTTAGCGCCGCCCA CTGAAGCAACGGTTAAGTAAGGTCTATAGTGGTTTAGGAGTCTTGCAACTCTATAATAATCCCTCCGCTAGTTCA CTAATAGAGACCTTGTT Aspergillus sp Sequence ID: FJ571436.1 Identities: 110/128(86%) >LPHT 233 233 5-4 ITS 5-ITS4 ITS 600 VT 1580 GCGAGGTGGTTGCTACTACGTTTAGGGTGATAGTGGGCATTGCCATTGGTTTTTGGGGCCTATAAGTAGCTGTAG GTCCCTAATATAAGCCTAGGGCTTGATAGTCGAAATGACGCTCAGATAGGTATACCCACTAGAATGCTGGTGAGT GTAATGTACGTTTAAAGATTCGATGATTTATAGAATTCTGCAATTTACATTACTTATTGTATTTCGCTATGTTCT Hoàng Văn Năm Page79 VIỆN ĐẠI HỌC MỔ HÀ NỘIKHOA CÔNG NGHỆ SINH HỌC TTATCGATACTAGAACTAAGAGATCCGTTATTAAAAGTTTTGACTTATTTAGTATAGAAGACTCAGAGAGGCCTT GTATATACGAGAATGGTTTAGAGCCTTTGGCAGGCGCCTTTGCAAAGGGGGCTAGGGGGCGCGCTAGTGAGCCTA TAGAGGCCCTAGCGCTACCTACTAAAACAACGGTTAGGTAAGGTCTATAGTGGTTTAGGAGTCTTACAACTCTGT AATGATCTCTCCGCTAGTTCACCAACGGAGACCTTGTTA Uncultured Cryptosporiopsis Sequence ID: FR774103 ^Identities: 116/130 (89%) >LPHT 234 234 5-4 ITS 5-ITS4 ITS 600 VT 1581 CCTTGGAAGATGTCTGACGGGTAGGCGGGCACCGGCCGGCACCTCCAGAGCGGGTGACAGAGCCCCATACGCTCA GGGACCGGAAACGCGGCACCGCCGCTGCCTTTCGGGCCCGTCCCTCCCCGCGGGAAGGGACGGCGGCCCAACACA CAAGCCGTGCTTGAGGGGCAGAAATGACGCTCGGACAGGCATGCCCCCCGGAATGCCAGAGGGCGCAATGTGCGT TCAAAGATTCGATGATTCACGGAATTCTGCAÃTTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCC GGAACCAAGAGATCCGTTGTTGAAAGTTTTGACGATTGTGTTTTCCACTCAGACCGCAACCTTTGTTCAAGACAA GCGTTCACGAGGGTCTCCGGCGGGCACGGGCCCGGAGGCACGGGAGATGGCCCCCCGGCGGCCAGGGACAGGAGC CCCCCTGGCGGGCCCGCCGAAGCAACACGGTACGGTAGACACGGGTGGGAGGTCGGGCCCGCAAAGGGGACCCTC ACTCGGTAATGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTAC Rasamsonia emersonii Sequence ID: JF417478.1Identities: 570/571 (99%) >LPHT 246 246 5-4 ITS 5-ITS4 ITS 600 VT 1582 GATTGACGGGTGGGCAGGCACCGGCCGGACCTCCAGAGCGGGTGACAGAGCCCCATACGCTCAAGGACCGGAACG CGGCACCGCCGCTGCCTTTCGGGCCCGTCCCCGAGGGGGACGGCGGCCCAACACACAAGCCGTGCTTGAGGGGCA GAAATGACGCTCGGACAGGCATGCCCCCCGGAATGCCAGAGGGCGCAATGTGCGTTCAAAGATTCGATGATTCAC GGAATTCTGCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGT TGAAAGTTTTGACGATTATGCTTTTCACTCAGACTGCAACCTTTGTTCAAGACAGCGTTCGCGAAGGTCTCCGGC GGGCGCGGGCCCGGGGGCGCGGTAGATGGCCCCCCGGCGGCCAGGGACAGAAGCCCCCGGCGGGCCCGCCGAAGC AACACGGTACAGTAGACACGGGTGGGAGGTCGGGCCCGCGAAGGGGACCCTCACTCAGTAATGATCCTTCCGCAG GTTCACCTACGGAAACCTTGTTAC Rasamsonia byssochlamydoides sequence Wft’mWiib&WHS! Nội >LPHT 251 251 5-4 ITS 5-ITS4 ITS 600 VT 1583 GTTGGGTGTCGGCTGGCGCCGGCCGGGCCTACAGAGCAGGTGACAAAGCCCCATACGCTCGAGGACCGGACGCGG TGCCGCCGCTGCCTTTCGGGCCCGTCCCCCGGGAGAGGGGGACGGGGGCCCAACACACAAGCCGTGCTTGAGGGC AGCAATGACGCTCGGACAGGCATGCCCCCCGGAATACCAGGGGGCGCAATGTGCGTTCAAAGACTCGATGATTCA CTGAATTCTGCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTG TTGAAAGTTTTAACTGATTACGATAATCAACTCAGACTGCATACTTTCAGAACAGCGTTCATGTTGGGGTCTTCG GCGGGCGCGGGCCCGGGGGCGCAAGGCCTCCCCGGCGGCCGTCGAAACGGCGGGCCCGCCGAAGCAACAAGGTAC GATAGACACGGGTGGGAGGTTGGACCCAGAGGGCCCTCACTCGGTAATGATCCTTCCGCAGGTTCACCTACGGAA ACCTTGTTACGA Aspergillus fumigatus Sequence ID: KP131574.1Identities: 537/537(100%) >LPHT 253 253 5-4 ITS 5-ITS4 ITS 600 VT 1584 GACCTTCAGAGGCTATGGCCCCGGCCGGACCCCTCCTTGAGCGCGTGACAGAGCCCCATACGCTCGAGGACCGGA CGCGACGCCGCCGCTGCCTTTCAGGCCCGTCCCCTCCAAACGAGGGGACGGCGGCCCAACACACAAGCCGTGCTT GAGGGTTCGCAATGACGCTCGGACAGGCATGCCCCCCGGAATACCAGAGGGCGCAATGTGCGTTCAAAGATTCGA TGATTCACGGAATTCTGCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGA TCCATTGTTGAAAGTTTTGAACGATTGCATTTCGTCACTCAGACAATCCTCGCATTCACAAAAGCGTTCACAGTG AGTGCCTCTGGCGGGCACGGGCCCCGGGGGCACACCGCGCCCCGGGACGACCCCCCCGGCGGAACACCGGAGGAG TGGGCCCGCCAAAGCAACACTAGGTGTGGTAGACACGGGTGGGAGATTGGGACCCGACTGGGTTCCCGCACTCGG TAATGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTA Thermomyces lanuginosus Sequence ID: NR_121309.1Identities: 565/565(100%) >LPHT 254 254 5-4 ITS 5-ITS4 ITS 600 VT 1585 GTTGGGTGTCGGCTGGCGCCGGCCGGGCCTACAGAGCAGGTGACAAAGCCCCATACGCTCGAGGACCGGACGCGG TGCCGCCGCTGCCTTTCGGGCCCGTCCCCCGGGAGAGGGGGACGGGGGCCCAACACACAAGCCGTGCTTGAGGAG CAATGACGCTCGGACAGGCATGCCCCCCGGAATACCAGGGGGCGCAATGTGCGTTCAAAGACTCGATGATTCACT Hoàng Văn Năm Page80 VIỆN ĐẠI HỌC MỔ HÀ NỘIKHOA CÔNG NGHỆ SINH HỌC GAATTCTGCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAACCAAGAGATCCGTTGTT GAAAGTTTTAACTGATTACGATAATCAACTCAGACTGCATACTTTCAGAACAGCGTTCATGTTGGGGTCTTCGGC GGGCGCGGGCCCGGGGGCGCAAGGCCTCCCCGGCGGCCGTCGAAÃCGGCGGGCCCGCCGAAGCAACAAGGTACGA TAGACACGGGTGGGAGGTTGGACCCAGAGGGCCCTCACTCGGTAATGATCCTTCCGCAGGTTCACCTACGGAAAC CTTGTTAC Aspergillus fumigatus Sequence ID: KP131574.1 Identities: 535/535(100%) >LPHT 269 269 5-4 ITS 5-ITS4 ITS 600 VT 1586 TCTGACCTTTGAGGCTATGGCCCCGGCCGGACCCCTCCTTGAGCGCGTGACAGAGCCCCATACGCTCGAGGACCG GACGCGACGCCGCCGCTGCCTTTCAGGCCCGTCCCCCCCCCCTGCAACGGGGGGACGGCGGCCCAACACACAAGC CGTGCTTGAGGGTTCGCAÃTGACGCTCGGACAGGCATGCCCCCCGGAATACCAGAGGGCGCAATGTGCGTTCAAA GATTCGATGATTCACGGAATTCTGCAATTCACATTACTTATCGCATTTCGCTGCGTTCTTCATCGATGCCGGAAC CAAGAGATCCATTGTTGAAAGTTTTGAACGATTGCATTTCATCACTCAGACAATCCTCGCATTCACAACAAGCAT TCACAGTGAGTGCCTCTGGCGGGCACGCGCCCCGGCAAAACCGCGCCCCGGGACGACCCCCCCGGCGGAGACCGG AGGAGTGGGCCCGCCAAAGCAACACCAGGTGTGGTAGACACGGGTGGGAGATTGGGACCCGATGGGGGTTCCCGC ACTCGGTAATGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTA Thermomyces dupontii Sequence ID: FJ548825.1Identities: 570/572(99%) >LPHT 224 224 5-4 ITS 1F-ITS4 ITS 600 VT 1587 TTATAAAAGTTTTTGGTTTTAAATTGGGGTTTCCCCAAACAATGGAAACCCCAAATTTCAAAATTTTAAAATTCC AAATAAGCCCCTTCAAAAAGGTCCCCCATCCAAAAAGGGCCAAAACCCCAAAACCGGTCCAATAACTTGGAAGGG GGAACCCAAAAAAAATTGGTTTTGCCCCCCCCCCCTCCACCCCCCAAAACCCAAAAATCCACAACAAAATTTAAT CCTTTGGGGTTCCAAAAẦAAACCGAACTAAACCTACCCCAGGGGAGAACCCAAGGGGGGAAAAAGGCTTCCAAAA TTT >LPHT 226 226 5-4 ITS 1F-ITS4 ITS 600 VT 1588 GTGTTCAAGAGTGGTTTGCACTTCAAACATAGCAAGTTTGGCAACATTGCTTCGAGATCAGCCTACTAAGTAAGC ACAGAAGCGATCCAAAGCACACAGGCGTGCCTTAGCATCGCTTACCAGTATTGCCGGAGCAACACCTAGAAGTCA AATGCAGTTGCAAAAGA^AGAGAAQTJTOCjrTZCEAACT|^ATG^I^C.TOCAAI]CAGATGAAACTAGTCTTGT aacggactagctgcattgcgcattagaatagtcgagt 6HtgttttatBsac^tagcatataacaccgattaacg TATCTAGCTACGTTCTTCATCGACCTGCGAGCCAAGTGATCCACCACTAATAGTTGTGAATTCGTTTTTCGTTTC AAAAGGCTAGAAATGGGTTTCGGTCGGCTGGAAACCATTCCAGGTCCGACCACGTTGCCCGGAGGCACCGTTCAT TTGGTTCATACATTAGTATGTATTTGGATTGATCCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTA CTTC Arthrobotrys arcuata Sequence ID: AF106527.lldentities: 61/64 (95%) >LPHT 236-1Đ 236 -1 IF.4 ITS 1F-ITS4 ITS 600 VT 1589 GAGGTGGTTGCTACTACGCTTAGGGTAATAGCGGGCATCGCCACTGGTTTTCGGGGCCTATAGACAGCTATAGGT CCCTAẦTATAAGCCTAGGGCTTGATAGTCGAAATAACGCTTAGATAGGTATATCCATCGGAATGCTGGCGAGCGC AATGTACGTTCAAAGATTCGATGATTTATAGAATTCTGCAATTTATATTATTTATCGTATTTCGCTATGTTCTTT ATCGATACTAGAACCAAGAGATCTATTGTTGAAAGTTTTGACTTATTCAGTATAGAAGACTTAGAGCAGCCTTAT ATATATAAGAATGGTTTAGAGCCTCTAGCGGGCGCCCCTGCGAGGGGGGCCGGGGGACACGCTAGTAGGCCTATA GGGGCCCTAGCGCCGCCTACCGAAGCAACAGTTAAATAAAGTCTACAGTGGTTTAGGAGTCTTGCAACTCTATAA TGATCCCTCCACTAGTTC Thielavia terrestris Sequence ID: CP003011.lldentities: 429/508 (84%) >LPHT 236-2T 236 -2 IF.4 ITS 1F-ITS4 ITS 600 VT 1590 TGCTTTCCATTTAACGGCTAGGACCTAGAGTATACCTTAAGCGAGGTGGTTGCTACTACGCTTAGGGTA ATAGCGGGCATCGCCACTGGTTTTCGGGGCCTATAGACAGCTATAGGTCCCTAATATAAGCCTAGGGCTTGATAG TCGAAATAACGCTTAGATAGGTATATCCATCGGAATGCTGGCGAGCGCAATGTACGTTCAAAGATTCGATGATTT ATAGAATTCTGCAATTTATATTATTTATCGTATTTCGCTATGTTCTTTATCGATACTAGAACCAAGAGATCTATT GTTGAAAGTTTTGACTTATTCAGTATAGAAGACTTAGAGCAGCCTTATATATATAAGAATGGTTTAGAGCCTCTA GCGGGCGCCCCTGCGAGGGGGGCCGGGGGACACGCTAGTAGGCCTATAGGGGCCCTAGCGCCGCCTACCGAAGCA ACAGTTAAATAAAGTCTACAGTGGTTTAGGAGTCTTGCAACTCTATAATGATCCCTCCACTAGTTCACCAACGGA GACCTTGTTACGACTTTTACTTCCTCT Thielavia terrestris Hoàng Văn Năm Page81 VIỆN ĐẠI HỌC MỚ HÀ NỘIKHOA CÔNG NGHỆ SINH HỌC Sequence ID: CP003011.lidentities: 457/546 (84%) >LPHT 236 236 1F-4 ITS1F-ITS4 ITS1F 600 VT 1656 GCCCCTATAGGCCTACTAGCGTGTCCCCCGGCCCCCCTCGCAGGGGCGCCCGCTAGAGGCTCTAAACCATTCTTA TATATATAAGGCTGCTCTAAGTCTTCTATACTGAATAAGTCAAAACTTTCAACAATAGATCTCTTGGTTCTAGTA TCGATAAAGAACATAGCGAAATACGATAAATAATATAAATTGCAGAATTCTATAAATCATCGAATCTTTGAACGT ACATTGCGCTCGCCAGCATTCCGATGGATATACCTATCTAAGCGTTATTTCGACTATCAAGCCCTAGGCTTATAT TAGGGACCTATAGCTGTCTATAGGCCCCGAAAACCAGTGGCGATGCCCGCTATTACCCTAAGCGTAGTAGCAACC ACCTCGCTTAAGGTATACTCTAGGTCCTAGCCGTTAAATGGAAAGCAACCCCCTAAAGGTTGACCTTGGATTAGG TAGGAGGACCCACTAAACTTAAGCATAT >LPH 216 216 IF.4 ITS 1F-ITS4 ITS 600 VT 1591 CAAGGTGGTTGCTACTACGCTCGGGGTGACAGTGGGCACCGCCACTGGTTTTCAGGGCCTGTAGGTAGCCGCAGG TCCCTAATACAAGCCTAGGGCTTGATGGTCGAAATGACGTTCGGATAGGTATACCCGCCGGAATACCGGCGGGCG CAATGTGCGTTCAAAGATTCGATGATTCATAGAATTCTGCAATTCACATTACTTATCGCATTTCGCTACGTTCTT TATCGATACTAGAACTAAGAGATCCGTTGTTGAAAGTTTTGACTCATTCAGTATAGAAGACTTAGAGAGGCCTTA TATATACTAAAATAGTTTGAAGCCTCTGGCGGGCGCCCCTGCGAGGGGGGCCGGGGGGCGCGCTAGTAGGCCTGT GGGGGCCCTGGCGCCTCCCACCCAAACAACCGTTAAGTAAAGTCCATAGTGGTTTAGGAATCTTGCAACTCTATA ATGATCCCTTCCCTGGTTCACCAACGGAAACCTTGTTACCACTTTTAC Thielavia terrestris Sequence ID: CP003011.1Identitie: 444/496 (90%) >LPH 216 216 IF ITS1F-ITS4 ITS1F 600 VT 1665 CGGAGGATCATTATAGAGTTGCAAGACTCCCAAACCACTATAGACCTTACCTAACCGTTGCTTCGGCAGGCAGCG CCAGGGCCCCTACAGGCCCGCTAACGCGCCCCCCGGCCCCCCTCGCGAGGGCGCCCGCCAAAGGCTCTAẦACCAT TCTCATATATACAAGGCCTCTCTAAGTCTTCTATACTGAATAAGTCAAAACTTTCAACAACGGATCTCTTAGTTC TAGCATCGATAAAGAACGTAGCGAAATGCAATAAGTAATATAAATTGCAGAATTCTATAAATTATCGAATCTTTA AACGTACATTGCGCCTACTAGCATTCCAGTGGGCATACCTATCCAAACGTCATTTCGACTATCAAGCCCCGAGCT TGTATTAAGGACCTACGGCTACCTATAGGCCCCGAAAACCAATAGCGGTACCCACTATCACCCTGAGCGTAATAG Thielavia terrestris Sequence ID: CP003011.1 Identities: 489/558(88%) Hoàng Văn Năm Page82 ... xylanase nấm mốc chịu nhiêt (pH 2.0,50 ) ph? ?n tích pH 3.0, pH 5.0, pH 7.0 Error! Bookmark not defined Hình 3.12 Hoạt tính xylanase chùng nấm mốc chịu pH thấp (pH 1.0, 30 °C) ph? ?n tích Ớ pH 3.0, pH. .. ph? ?n mạnh nguồn giàu lignocellulose từ môi trường khác nham đáp ứng nhu cầu thực tiền đời sống với đề tài: ? ?Ph? ?n lập nấm mốc chịu nhiệt, chịu pH thấp? ?? Muc tiêu: Tìm kiếm, ph? ?n lập chủng nấm mốc. .. lU/ml, LPH245 6,26 lU/ml, thấp LPHT 228 Với pH 7.0 cao LPH 254 3,24 lU/ml Với chủng nấm mốc chịu pH thấp (pH 1.0, 30 °C) hoạt tính CMCase dái pH 3.0, pH 5.0 đồng (Hình 3.10) cao Cũng chủng nấm mốc