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TIÊU HÓA IN VITRO ARGONChuyên ngành : : 60 54 02 12 2013 i C HOÀN THÀNH T I I H C BÁCH KHOA Cán b - HCM ng d n khoa h c : TS Tr nh Khánh S n Cán b ch m nh n xét : GS TS n Cán b ch m nh n xét : TS Phan T i Huân Lu Thành ph n H i Ch t ch H c b o v t i Tr ng ng i h c Bách Khoa, HQG Tp HCM nh giá lu n v n th c s g m: ng: PGS TS Ngô K Ph n bi n Ph n bi n 2: TS Phan T i Huân y viên: TS Tr nh Khá n Th Ng c Yên Xác nh n c a Ch t ch H i ngành sau lu n v n ã CH T CH H NG ng h giá LV c s a ch a (n u có) ng Khoa qu n lý chuyên NG KHOA K THU T HOÁ H C ii I H C QU C GIA TP.HCM C NG HÒA XÃ H I CH I H C BÁCH KHOA T NAM c l p - T - H nh phúc NHI M V LU 08 An : 60 54 02 I TÀI: Nghiên c u s bi n tính s iv tiêu hóa in vitro c a tinh b t b p s n d ng h t qua trình x lý Argon-plasma áp su ng II NHI M V VÀ N I DUNG: Nghiên c u trình bi n tính tinh b t b p, s n d ng h t b ng Argon-plasma nh m t o tinh b t liên k t ngang i v c u trúc hóa h m hình thái, màu s c kh n nhi t c a tinh b c sau x lý Argon-plasma áp su ng thông Nghiên c tiêu hóa in vitro c a tinh b c sau x lý Argon-plasma áp su ng Mơ t m hình thái c a tinh b t sau enzyme tiêu hóa t n cơng b ng kính hi n t quét (SEM) III NGÀY GIAO NHI M V : 24-06-2013 IV NGÀY HOÀN THÀNH NHI M V : 22-11-2013 V CÁN B NG D N: TS Tr Tp HCM CÁN B NG D N (H tên ch ký) CH NHI M B (H tên ch ký) NG KHOA K THU T HOÁ H C (H tên ch ký) O iii Tp H iv ABSTRACT Tapioca and maize starches have been widely used in the food industry However, native starches have limited in industrial applications Thus, food scientists try to modify the properties of starch such as heat stability, theology, es were treated by atmospheric Argon-plasma from to 40 minutes at to KV Structural properties of starch samples were characterized using FTIR spectroscopy and thermalgravimetric analyzer The positive correlations between the degree of crosslinking, morphological properties and digestibility (especially in RS content) were investigated The results showed that the highest degree of cross-linking of treated tapioca starch (2.47 times higher than that of native starch), was reached under 10 minutes, and kW of treatment Besides, the highest degree of cross-linking of treated maize starch, two times higher than that of native, were reached under 20 minutes, and kW of treatment The morphology of treated tapioca and maize starches showed slight changes in shape, sizes and colour compared with that of native starch The behavior of native and treated starches under enzymatic digestion was obviously different depending on starch sources Argon-plasma treatment prevents starch granules from the digestion reflected in high content of resistant starch In summary, atmospheric Argon-plasma treatment is possible for heat stable RS production v - - Argon- vi in vitro - vii ii iii ABSTRACT iv v vi vii x xi xii xiii .1 .2 .4 .4 .9 10 2.3 10 11 11 13 13 14 15 .15 15 16 18 RDS) 19 viii SDS) 19 RS) .19 .21 -plasma 21 in vitro 22 24 24 24 .24 24 3.2.3 Enzyme 25 25 29 30 .30 31 3.5.3 Kh o sát ng c a liên k n kh u nhi t c a m u tinh b t s n, b p 32 3.5.4 Kh o sát ng c a x lý Argonm hình thái c a tinh b t s n, b p 33 3.5.5 Kh o sát ng c a x lý Argon-plasma lên màu s c c a tinh b t s n, b p 34 3.5.6 in vitro pancreatin amyloglucosidase 34 3.5.7 Kh o sát ng c a enzyme tiêu hóa m hình thái c a tinh b t s n, b p 35 36 rgon 36 36 .39 39 42 45 ix 45 .47 49 49 51 in vitro 53 in vitro 53 in vitro 53 4.2.2.2 M 55 58 4.2.2.1 Tin 58 .59 61 5.1 K t lu n 61 5.2 Ki n ngh 61 63 Th i gian 20 phút ANOVA Table for LKN by DIEN AP Source Sum of Squares Between groups 0.154109 Within groups 0.00604333 Total (Corr.) 0.160152 Df 12 17 Mean Square 0.0308218 0.000503611 Multiple Range Tests for LKN by DIEN AP Method: 95.0 percent LSD DIEN AP Count Mean Homogeneous Groups 0.639333 X 0.654667 XX X 0.690333 X 0.692 X 0.736333 X 0.916667 F-Ratio 61.20 Contrast 4-5 4-6 4-7 4-8 4-9 5-6 5-7 5-8 5-9 6-7 6-8 6-9 7-8 7-9 8-9 P-Value 0.0000 Sig * * * * * * * * * * * Difference -0.0443333 -0.224667 0.00166667 0.0373333 0.0526667 -0.180333 0.046 0.0816667 0.097 0.226333 0.262 0.277333 0.0356667 0.051 0.0153333 + /- Limits 0.039923 0.039923 0.039923 0.039923 0.039923 0.039923 0.039923 0.039923 0.039923 0.039923 0.039923 0.039923 0.039923 0.039923 0.039923 * denotes a statistically significant difference Th i gian 30 phút ANOVA Table for LKN by DIEN AP Source Sum of Squares Between groups 0.0744312 Within groups 0.00320133 Total (Corr.) 0.0776325 Df 12 17 Mean Square 0.0148862 0.000266778 Multiple Range Tests for LKN by DIEN AP Method: 95.0 percent LSD DIEN AP Count Mean Homogeneous Groups 0.479333 X X 0.554333 3 3 0.598333 0.640667 0.657 0.657333 X X X X F-Ratio 55.80 Contrast 4-5 4-6 4-7 4-8 4-9 5-6 5-7 5-8 5-9 6-7 6-8 6-9 7-8 7-9 8-9 P-Value 0.0000 Sig * * * * * * * * * * * * Difference -0.0586667 -0.059 0.044 0.119 -0.0423333 -0.000333333 0.102667 0.177667 0.0163333 0.103 0.178 0.0166667 0.075 -0.0863333 -0.161333 + /- Limits 0.029057 0.029057 0.029057 0.029057 0.029057 0.029057 0.029057 0.029057 0.029057 0.029057 0.029057 0.029057 0.029057 0.029057 0.029057 * denotes a statistically significant difference Th i gian 40 phút ANOVA Table for LKN by DIEN AP Source Sum of Squares Between groups 0.0913025 Within groups 0.003416 Total (Corr.) 0.0947185 Df 12 17 Mean Square 0.0182605 0.000284667 Multiple Range Tests for LKN by DIEN AP Method: 95.0 percent LSD DIEN AP Count Mean Homogeneous Groups 0.464667 X Contrast 4-5 4-6 4-7 4-8 4-9 5-6 5-7 5-8 5-9 6-7 6-8 6-9 7-8 7-9 8-9 X 0.53 0.572 X 0.582667 X 0.655667 X 0.674 X F-Ratio 64.15 P-Value 0.0000 Sig * * * * * * * * * * * * * Difference 0.0836667 -0.0183333 0.191 0.125667 0.073 -0.102 0.107333 0.042 -0.0106667 0.209333 0.144 0.0913333 -0.0653333 -0.118 -0.0526667 + /- Limits 0.0300154 0.0300154 0.0300154 0.0300154 0.0300154 0.0300154 0.0300154 0.0300154 0.0300154 0.0300154 0.0300154 0.0300154 0.0300154 0.0300154 0.0300154 * denotes a statistically significant difference 2.2 nh 929 cm-1 ANOVA Table for DINH 929 by NT Source Sum of Squares Between groups 169312 Within groups 0287 Total (Corr.) 198012 Df 10 14 Mean Square 0423281 00287 Multiple Range Tests for DINH 929 by NT Method: 95.0 percent LSD NT Count Mean Homogeneous Groups DC 370766 X T-20P-8K T-10P-6K 480798 518255 T-20P-6K T-10P-8K 579816 691423 X XX X X F-Ratio 14.75 P-Value Contrast Sig Difference DC - T-10P-6K * -.147489 DC - T-10P-8K * -.320658 DC - T-20P-6K * -.20905 DC - T-20P-8K * -.110033 T-10P-6K - T-10P-8K * -.173168 T-10P-6K - T-20P-6K -.061561 T-10P-6K - T-20P-8K 0374567 T-10P-8K - T-20P-6K * 111607 T-10P-8K - T-20P-8K * 210625 T-20P-6K - T-20P-8K * 0990177 * denotes a statistically significant difference + /- Limits 0974627 0974627 0974627 0974627 0974627 0974627 0974627 0974627 0974627 0974627 nh 1016 cm-1 ANOVA Table for DINH 1016 by NT Source Sum of Squares Between groups 1.05728 Within groups 025444 Total (Corr.) 1.08273 Df 10 14 Mean Square 26432 0025444 Multiple Range Tests for DINH 1016 by NT Method: 95.0 percent LSD NT Count Mean Homogeneous Groups X DC 8914 X T-20P-8K 1.25291 T-20P-6K 1.35965 X T-10P-6K 1.43003 X T-10P-8K 1.70847 X F-Ratio 103.88 Contrast DC - T-10P-6K DC - T-10P-8K DC - T-20P-6K DC - T-20P-8K T-10P-6K - T-10P-8K T-10P-6K - T-20P-6K T-10P-6K - T-20P-8K T-10P-8K - T-20P-6K T-10P-8K - T-20P-8K T-20P-6K - T-20P-8K P-Value Sig * * * * * * * * * Difference -.538626 -.817072 -.468249 -.361509 -.278446 0703771 177118 348823 455563 10674 + /- Limits 0917678 0917678 0917678 0917678 0917678 0917678 0917678 0917678 0917678 0917678 * denotes a statistically significant difference nh 1244 cm-1 ANOVA Table for DINH 1244 by NT Source Sum of Squares Between groups 129618 Within groups 024276 Total (Corr.) 153894 Df 10 14 Multiple Range Tests for DINH 1244 by NT Method: 95.0 percent LSD NT Count Mean Homogeneous Groups DC 357087 X T-20P-8K 454252 X T-10P-6K 489441 XX T-20P-6K 552853 XX T-10P-8K 633481 X Mean Square 0324045 0024276 F-Ratio 13.35 P-Value Contrast Sig Difference DC - T-10P-6K * -.132354 DC - T-10P-8K * -.276394 DC - T-20P-6K * -.195766 DC - T-20P-8K * -.0971645 T-10P-6K - T-10P-8K * -.144041 T-10P-6K - T-20P-6K -.0634123 T-10P-6K - T-20P-8K 0351891 T-10P-8K - T-20P-6K 0806283 T-10P-8K - T-20P-8K * 17923 T-20P-6K - T-20P-8K * 0986015 * denotes a statistically significant difference nh 1647 cm-1 Source Between groups Within groups Total (Corr.) Sum of Squares 139939 0248607 164799 Df 10 14 Mean Square 0349846 00248607 F-Ratio 14.07 P-Value + /- Limits 0896368 0896368 0896368 0896368 0896368 0896368 0896368 0896368 0896368 0896368 Multiple Range Tests for DINH 1647 by NT Method: 95.0 percent LSD NT Count Mean Homogeneous Groups X T-20P-8K 31679 DC 349644 X T-10P-6K T-20P-6K 3 400464 509992 X X T-10P-8K 571782 X Contrast Sig Difference DC - T-10P-6K -.0508196 DC - T-10P-8K * -.222137 DC - T-20P-6K * -.160347 DC - T-20P-8K 0328539 T-10P-6K - T-10P-8K * -.171318 T-10P-6K - T-20P-6K * -.109528 T-10P-6K - T-20P-8K 0836735 T-10P-8K - T-20P-6K 0617899 T-10P-8K - T-20P-8K * 254991 T-20P-6K - T-20P-8K * 193201 * denotes a statistically significant difference + /- Limits 0907098 0907098 0907098 0907098 0907098 0907098 0907098 0907098 0907098 0907098 nh 3282 cm-1 ANOVA Table for DINH 3282 by NT Source Sum of Squares Between groups 349205 Within groups 023034 Total (Corr.) 372239 Df 10 14 Multiple Range Tests for DINH 3282 by NT Method: 95.0 percent LSD NT Count Mean Homogeneous Groups DC 285352 X T-10P-6K 526042 X T-20P-8K 549927 X T-20P-6K 576115 X T-10P-8K 763514 X Mean Square 0873013 0023034 F-Ratio 37.90 Contrast DC - T-10P-6K DC - T-10P-8K DC - T-20P-6K DC - T-20P-8K T-10P-6K - T-10P-8K T-10P-6K - T-20P-6K T-10P-6K - T-20P-8K T-10P-8K - T-20P-6K T-10P-8K - T-20P-8K T-20P-6K - T-20P-8K P-Value Sig * * * * * * * Difference -.240691 -.478162 -.290764 -.264575 -.237472 -.0500731 -.0238842 187399 213587 0261889 * denotes a statistically significant difference nh 929 cm-1 ANOVA Table for DINH 929 by NT Source Sum of Squares Between groups 283331 Within groups 019974 Total (Corr.) 303305 Df 10 14 Mean Square 0708328 0019974 F-Ratio 35.46 P-Value + /- Limits 0873137 0873137 0873137 0873137 0873137 0873137 0873137 0873137 0873137 0873137 Multiple Range Tests for DINH 929 by NT Method: 95.0 percent LSD NT Count Mean Homogeneous Groups M-10P-5K 405866 X DC 412489 X M-20P-5K 591772 X M-10P-6K 598272 X M-20P-6K 775647 X Contrast DC - M-10P-5K Sig Difference 00662334 + /- Limits 0813074 DC - M-10P-6K DC - M-20P-5K * * -.185783 -.179283 0813074 0813074 DC - M-20P-6K M-10P-5K - M-10P-6K * * -.363158 -.192406 0813074 0813074 M-10P-5K - M-20P-5K M-10P-5K - M-20P-6K * * -.185906 -.369781 0813074 0813074 M-10P-6K - M-20P-5K M-10P-6K - M-20P-6K * 00650002 -.177375 0813074 0813074 M-20P-5K - M-20P-6K * -.183875 0813074 * denotes a statistically significant difference nh 1016 cm-1 ANOVA Table for DINH 1016 by NT Source Sum of Squares Between groups 1.21632 Within groups 0146388 Total (Corr.) 1.23096 Df 10 14 Multiple Range Tests for DINH 1016 by NT Method: 95.0 percent LSD NT Count Mean Homogeneous Groups DC 984451 X M-10P-5K 1.14866 X M-10P-6K 1.48364 M-20P-5K 1.6344 M-20P-6K 1.72823 X X X Mean Square 30408 00146388 F-Ratio 207.72 P-Value Contrast Sig Difference DC - M-10P-5K * -.164212 DC - M-10P-6K * -.499185 DC - M-20P-5K * -.649954 DC - M-20P-6K * -.743775 M-10P-5K - M-10P-6K * -.334974 M-10P-5K - M-20P-5K * -.485742 M-10P-5K - M-20P-6K * -.579564 M-10P-6K - M-20P-5K * -.150768 M-10P-6K - M-20P-6K * -.24459 M-20P-5K - M-20P-6K * -.0938217 * denotes a statistically significant difference nh 1242 cm-1 ANOVA Table for DINH 1242 by NT Source Sum of Squares Between groups 16343 Within groups 03056 Total (Corr.) 19399 Df 10 14 Mean Square 0408576 003056 F-Ratio 13.37 P-Value + /- Limits 0696065 0696065 0696065 0696065 0696065 0696065 0696065 0696065 0696065 0696065 Multiple Range Tests for DINH 1242 by NT Method: 95.0 percent LSD NT Count Mean Homogeneous Groups M-10P-5K 386071 X DC 393334 X M-20P-5K 567976 X M-10P-6K 569293 X M-20P-6K 646624 X Contrast DC - M-10P-5K DC - M-10P-6K DC - M-20P-5K DC - M-20P-6K M-10P-5K - M-10P-6K M-10P-5K - M-20P-5K M-10P-5K - M-20P-6K M-10P-6K - M-20P-5K M-10P-6K - M-20P-6K M-20P-5K - M-20P-6K Sig * * * * * * Difference 00726285 -.175959 -.174641 -.25329 -.183222 -.181904 -.260552 00131756 -.0773306 -.0786481 + /- Limits 100571 100571 100571 100571 100571 100571 100571 100571 100571 100571 * denotes a statistically significant difference nh 1647 cm-1 ANOVA Table for DINH 1647 by NT Source Sum of Squares Between groups 1089 Within groups 024756 Total (Corr.) 133656 Df 10 14 Multiple Range Tests for DINH 1647 by NT Method: 95.0 percent LSD NT Count Mean Homogeneous Groups M-10P-5K 311377 X DC 338281 X M-20P-5K M-10P-6K 440343 482272 X XX M-20P-6K 53639 X Mean Square 027225 0024756 F-Ratio 11.00 P-Value 11 Contrast DC - M-10P-5K DC - M-10P-6K DC - M-20P-5K DC - M-20P-6K M-10P-5K - M-10P-6K M-10P-5K - M-20P-5K M-10P-5K - M-20P-6K M-10P-6K - M-20P-5K M-10P-6K - M-20P-6K M-20P-5K - M-20P-6K Sig * * * * * * * Difference 0269041 -.14399 -.102061 -.198108 -.170894 -.128965 -.225012 0419291 -.0541182 -.0960473 * denotes a statistically significant difference nh 3242 cm-1 ANOVA Table for DINH 3242 by NT Source Sum of Squares Between groups 693929 Within groups 030378 Total (Corr.) 724307 Df 10 14 Mean Square 173482 0030378 F-Ratio 57.11 P-Value + /- Limits 0905186 0905186 0905186 0905186 0905186 0905186 0905186 0905186 0905186 0905186 Multiple Range Tests for DINH 3242 by NT Method: 95.0 percent LSD NT Count Mean Homogeneous Groups DC 346838 X M-10P-5K 399403 X M-10P-6K M-20P-6K 698034 785643 M-20P-5K 893146 Contrast DC - M-10P-5K DC - M-10P-6K DC - M-20P-5K DC - M-20P-6K M-10P-5K - M-10P-6K M-10P-5K - M-20P-5K M-10P-5K - M-20P-6K M-10P-6K - M-20P-5K M-10P-6K - M-20P-6K M-20P-5K - M-20P-6K X X X Sig * * * * * * * * Difference -.0525651 -.351196 -.546308 -.438805 -.298631 -.493743 -.38624 -.195112 -.0876095 107503 * denotes a statistically significant difference Giá tr L ANOVA Table for L by NT Source Sum of Squares Between groups 260417 Within groups 376667 Total (Corr.) 637083 Df Mean Square 260417 0941667 F-Ratio 2.77 P-Value 1717 F-Ratio 12 P-Value 7515 Multiple Range Tests for L by NT Method: 95.0 percent LSD NT Count Mean DC 48.5833 Homogeneous Groups X T-10P-8K X 49.0 Contrast Sig DC T-10P-8K Difference -.416667 + /- Limits 695654 * denotes a statistically significant difference Gía tr a ANOVA Table for a by NT Source Sum of Squares Between groups 000266667 Within groups 00926667 Total (Corr.) 00953333 Df Mean Square 000266667 00231667 Multiple Range Tests for a by NT Method: 95.0 percent LSD NT Count T-10P-8K Mean 32 Homogeneous Groups X DC 333333 X Contrast Sig Difference DC T-10P-8K 0133333 * denotes a statistically significant difference + /- Limits 109113 + /- Limits 100271 100271 100271 100271 100271 100271 100271 100271 100271 100271 Gía tr b ANOVA Table for b by NT Source Sum of Squares Between groups 0096 Within groups 00713333 Total (Corr.) 0167333 Multiple Range Tests for b by NT Method: 95.0 percent LSD NT Count DC T-10P-8K Contrast DC T-10P-8K Sig Df Mean 993333 1.07333 Mean Square 0096 00178333 F-Ratio 5.38 P-Value 811 Homogeneous Groups X X Difference -.08 + /- Limits 0957329 * denotes a statistically significant difference Giá tr L ANOVA Table for L by NT Source Sum of Squares Between groups 0266667 Within groups 2.69853 Total (Corr.) 2.7252 Df Mean Square 0266667 674633 F-Ratio P-Value 8521 Multiple Range Tests for L by NT Method: 95.0 percent LSD NT Count DC M-20P-6K Contrast DC - M-20P-6K Sig Mean 50.2533 50.3867 Homogeneous Groups X X Difference -.133333 + /- Limits 1.862 * denotes a statistically significant difference Gía tr b ANOVA Table for b by NT Source Sum of Squares Between groups 000816667 Within groups 0321333 Total (Corr.) 03295 Multiple Range Tests for b by NT Method: 95.0 percent LSD NT Count Mean M-20P-6K 2.24333 DC 2.26667 Contrast Sig DC - M-20P-6K Df Mean Square 000816667 00803333 F-Ratio 10 Homogeneous Groups X X Difference 0233333 * denotes a statistically significant difference + /- Limits 203185 P-Value 7658 in vitro hóa RDS ANOVA Table for RDS by nt Source Sum of Squares Between groups 907.4 Within groups 0.5006 Total (Corr.) 907.9 Df 10 14 Mean Square 226.85 0.05006 F-Ratio 4531.56 P-Value 0.0000 Multiple Range Tests for RDS by nt Method: 95.0 percent LSD nt Count Mean T-10P-8K 30.37 T-10P-6K T-20P-8K T-20P-6K T-THO 32.85 43.8767 47.4233 49.5833 3 3 Homogeneous Groups X X X X X Contrast T-10P-6K - T-10P-8K T-10P-6K - T-20P-6K T-10P-6K - T-20P-8K T-10P-6K - T-THO T-10P-8K - T-20P-6K T-10P-8K - T-20P-8K T-10P-8K - T-THO T-20P-6K - T-20P-8K T-20P-6K - T-THO T-20P-8K - T-THO Sig * * * * * * * * * * Difference 2.48 -14.5733 -11.0267 -16.7333 -17.0533 -13.5067 -19.2133 3.54667 -2.16 -5.70667 + /- Limits 0.407046 0.407046 0.407046 0.407046 0.407046 0.407046 0.407046 0.407046 0.407046 0.407046 * denotes a statistically significant difference SDS ANOVA Table for SDS by nt Source Sum of Squares Between groups 333.449 Within groups 2.2298 Total (Corr.) 335.678 Multiple Range Tests for SDS by nt Method: 95.0 percent LSD nt Count Mean Df 10 14 T-10P-6K T-20P-8K Homogeneous Groups 9.74667 X X 10.8 X 13.2833 T-20P-6K T-THO 15.54 23.0033 T-10P-8K X X Mean Square 83.3621 0.22298 F-Ratio 373.85 Contrast T-10P-6K - T-10P-8K T-10P-6K - T-20P-6K T-10P-6K - T-20P-8K T-10P-6K - T-THO T-10P-8K - T-20P-6K T-10P-8K - T-20P-8K T-10P-8K - T-THO T-20P-6K - T-20P-8K T-20P-6K - T-THO T-20P-8K - T-THO P-Value 0.0000 Sig * * * * * * * * * * Difference 1.05333 -4.74 -2.48333 -12.2033 -5.79333 -3.53667 -13.2567 2.25667 -7.46333 -9.72 * denotes a statistically significant difference + /- Limits 0.859074 0.859074 0.859074 0.859074 0.859074 0.859074 0.859074 0.859074 0.859074 0.859074 RS ANOVA Table for RS by nt Source Sum of Squares Between groups 2181.81 Within groups 1.143 Total (Corr.) 2182.96 Df 10 14 Mean Square 545.453 0.1143 Multiple Range Tests for RS by nt Method: 95.0 percent LSD nt Count Mean Homogeneous Groups T-THO 27.4133 X T-20P-6K T-20P-8K 37.0367 42.84 T-10P-6K T-10P-8K 56.35 59.8833 X X X X F-Ratio 4772.12 Contrast T-10P-6K - T-10P-8K T-10P-6K - T-20P-6K T-10P-6K - T-20P-8K T-10P-6K - T-THO T-10P-8K - T-20P-6K T-10P-8K - T-20P-8K T-10P-8K - T-THO T-20P-6K - T-20P-8K T-20P-6K - T-THO T-20P-8K - T-THO P-Value 0.0000 Sig * * * * * * * * * * Difference -3.53333 19.3133 13.51 28.9367 22.8467 17.0433 32.47 -5.80333 9.62333 15.4267 + /- Limits 0.615065 0.615065 0.615065 0.615065 0.615065 0.615065 0.615065 0.615065 0.615065 0.615065 * denotes a statistically significant difference hóa RDS ANOVA Table for RDS by nt Source Sum of Squares Between groups 449.027 Within groups 1.19247 Total (Corr.) 450.22 Multiple Range Tests for RDS by nt Df 10 14 Method: 95.0 percent LSD nt Count Mean T-20P-8K T-20P-6K Homogeneous Groups 41.0733 X 45.4167 X X 48.66 X 51.7167 T-THO 57.1667 T-10P-8K T-10P-6K 3 X Mean Square 112.257 0.119247 F-Ratio 941.38 Contrast T-10P-6K - T-10P-8K T-10P-6K - T-20P-6K T-10P-6K - T-20P-8K T-10P-6K - T-THO T-10P-8K - T-20P-6K T-10P-8K - T-20P-8K T-10P-8K - T-THO T-20P-6K - T-20P-8K T-20P-6K - T-THO T-20P-8K - T-THO P-Value 0.0000 Sig * * * * * * * * * * Difference 4.34333 -6.3 -3.24333 -11.75 -10.6433 -7.58667 -16.0933 3.05667 -5.45 -8.50667 * denotes a statistically significant difference SDS ANOVA Table for SDS by nt Source Sum of Squares Between groups 618.875 Within groups 6.58233 Total (Corr.) 625.458 Df 10 14 Mean Square 154.719 0.658233 F-Ratio 235.05 P-Value 0.0000 + /- Limits 0.628233 0.628233 0.628233 0.628233 0.628233 0.628233 0.628233 0.628233 0.628233 0.628233 Multiple Range Tests for SDS by nt Method: 95.0 percent LSD nt Count Mean Homogeneous Groups X T-10P-8K 6.56 T-20P-8K 19.2067 X X T-10P-6K 20.7733 T-20P-6K T-THO 21.35 25.58 Contrast T-10P-6K - T-10P-8K T-10P-6K - T-20P-6K T-10P-6K - T-20P-8K T-10P-6K - T-THO T-10P-8K - T-20P-6K T-10P-8K - T-20P-8K T-10P-8K - T-THO T-20P-6K - T-20P-8K T-20P-6K - T-THO T-20P-8K - T-THO X X Sig * * * * * * * * * Difference 14.2133 -0.576667 1.56667 -4.80667 -14.79 -12.6467 -19.02 2.14333 -4.23 -6.37333 + /- Limits 1.476 1.476 1.476 1.476 1.476 1.476 1.476 1.476 1.476 1.476 * denotes a statistically significant difference RS ANOVA Table for RS by nt Source Sum of Squares Between groups 1979.95 Within groups 3.71587 Total (Corr.) 1983.67 Df 10 14 Mean Square 494.988 0.371587 Multiple Range Tests for RS by nt Method: 95.0 percent LSD nt Count Mean Homogeneous Groups T-THO 17.2533 X T-20P-6K T-20P-8K 26.9333 32.1333 T-10P-6K T-10P-8K 33.81 52.3667 X X X X F-Ratio 1332.09 Contrast T-10P-6K - T-10P-8K T-10P-6K - T-20P-6K T-10P-6K - T-20P-8K T-10P-6K - T-THO T-10P-8K - T-20P-6K T-10P-8K - T-20P-8K T-10P-8K - T-THO T-20P-6K - T-20P-8K T-20P-6K - T-THO T-20P-8K - T-THO P-Value 0.0000 Sig * * * * * * * * * * Difference -18.5567 6.87667 1.67667 16.5567 25.4333 20.2333 35.1133 -5.2 9.68 14.88 * denotes a statistically significant difference hóa RDS ANOVA Table for RDS by nt Source Sum of Squares Between groups 637.188 Within groups 0.727467 Total (Corr.) 637.916 Df 10 14 Mean Square 159.297 0.0727467 F-Ratio 2189.75 P-Value 0.0000 + /- Limits 1.10899 1.10899 1.10899 1.10899 1.10899 1.10899 1.10899 1.10899 1.10899 1.10899 Multiple Range Tests for RDS by nt Method: 95.0 percent LSD nt Count Mean Homogeneous Groups X M-20P-6K 31.56 X M-20P-5K 42.81 X M-10P-6K 46.35 M-10P-5K M-THO X X 47.89 49.9967 Contrast M-10P-5K - M-10P-6K M-10P-5K - M-20P-5K M-10P-5K - M-20P-6K M-10P-5K - M-THO M-10P-6K - M-20P-5K M-10P-6K - M-20P-6K M-10P-6K - M-THO M-20P-5K - M-20P-6K M-20P-5K - M-THO M-20P-6K - M-THO Sig * * * * * * * * * * Difference 1.54 5.08 16.33 -2.10667 3.54 14.79 -3.64667 11.25 -7.18667 -18.4367 + /- Limits 0.490687 0.490687 0.490687 0.490687 0.490687 0.490687 0.490687 0.490687 0.490687 0.490687 * denotes a statistically significant difference SDS ANOVA Table for SDS by nt Source Sum of Squares Between groups 512.125 Within groups 6.46187 Total (Corr.) 518.586 Df 10 14 Multiple Range Tests for SDS by nt Method: 95.0 percent LSD nt Count Mean Homogeneous Groups X M-20P-6K 10.5 M-20P-5K 18.4933 M-10P-6K M-10P-5K M-THO 20.2933 22.0633 28.5933 X X X X Mean Square 128.031 0.646187 F-Ratio 198.13 Contrast M-10P-5K - M-10P-6K M-10P-5K - M-20P-5K M-10P-5K - M-20P-6K M-10P-5K - M-THO M-10P-6K - M-20P-5K M-10P-6K - M-20P-6K M-10P-6K - M-THO M-20P-5K - M-20P-6K M-20P-5K - M-THO M-20P-6K - M-THO P-Value 0.0000 Sig * * * * * * * * * * Difference 1.77 3.57 11.5633 -6.53 1.8 9.79333 -8.3 7.99333 -10.1 -18.0933 * denotes a statistically significant difference RS ANOVA Table for RS by nt Source Sum of Squares Between groups 2230.54 Within groups 4.4298 Total (Corr.) 2234.97 Df 10 14 Mean Square 557.634 0.44298 F-Ratio 1258.83 P-Value 0.0000 + /- Limits 1.46244 1.46244 1.46244 1.46244 1.46244 1.46244 1.46244 1.46244 1.46244 1.46244 Multiple Range Tests for RS by nt Method: 95.0 percent LSD nt Count Mean Homogeneous Groups X M-THO 21.41 M-10P-5K 30.0467 X X M-10P-6K 33.3567 M-20P-5K M-20P-6K 38.6967 57.94 X X Contrast M-10P-5K - M-10P-6K M-10P-5K - M-20P-5K M-10P-5K - M-20P-6K M-10P-5K - M-THO M-10P-6K - M-20P-5K M-10P-6K - M-20P-6K M-10P-6K - M-THO M-20P-5K - M-20P-6K M-20P-5K - M-THO M-20P-6K - M-THO Sig * * * * * * * * * * Difference -3.31 -8.65 -27.8933 8.63667 -5.34 -24.5833 11.9467 -19.2433 17.2867 36.53 + /- Limits 1.21085 1.21085 1.21085 1.21085 1.21085 1.21085 1.21085 1.21085 1.21085 1.21085 * denotes a statistically significant difference hóa RDS ANOVA Table for RDS by nt Source Sum of Squares Between groups 853.822 Within groups 0.924733 Total (Corr.) 854.747 Df 10 14 Multiple Range Tests for RDS by nt Method: 95.0 percent LSD nt Count Mean Homogeneous Groups M-20P-6K 34.9567 X X M-20P-5K 44.29 M-10P-6K M-10P-5K 48.91 51.89 M-THO 57.2833 X X X Mean Square 213.456 0.0924733 F-Ratio 2308.29 Contrast M-10P-5K - M-10P-6K M-10P-5K - M-20P-5K M-10P-5K - M-20P-6K M-10P-5K - M-THO M-10P-6K - M-20P-5K M-10P-6K - M-20P-6K M-10P-6K - M-THO M-20P-5K - M-20P-6K M-20P-5K - M-THO M-20P-6K - M-THO P-Value 0.0000 Sig * * * * * * * * * * Difference 2.98 7.6 16.9333 -5.39333 4.62 13.9533 -8.37333 9.33333 -12.9933 -22.3267 * denotes a statistically significant difference SDS ANOVA Table for SDS by nt Source Sum of Squares Between groups 430.58 Within groups 1.17393 Total (Corr.) 431.754 Df 10 14 Mean Square 107.645 0.117393 F-Ratio 916.96 P-Value 0.0000 + /- Limits 0.55323 0.55323 0.55323 0.55323 0.55323 0.55323 0.55323 0.55323 0.55323 0.55323 Multiple Range Tests for SDS by nt Method: 95.0 percent LSD nt Count Mean Homogeneous Groups M-20P-6K 10.1567 X X M-20P-5K 17.1 X M-10P-6K 18.2467 M-10P-5K M-THO 20.28 26.79 X X Contrast M-10P-5K - M-10P-6K M-10P-5K - M-20P-5K M-10P-5K - M-20P-6K M-10P-5K - M-THO M-10P-6K - M-20P-5K M-10P-6K - M-20P-6K M-10P-6K - M-THO M-20P-5K - M-20P-6K M-20P-5K - M-THO M-20P-6K - M-THO Sig * * * * * * * * * * Difference 2.03333 3.18 10.1233 -6.51 1.14667 8.09 -8.54333 6.94333 -9.69 -16.6333 + /- Limits 0.623332 0.623332 0.623332 0.623332 0.623332 0.623332 0.623332 0.623332 0.623332 0.623332 * denotes a statistically significant difference RS ANOVA Table for RS by nt Source Sum of Squares Between groups 2470.67 Within groups 0.955 Total (Corr.) 2471.62 Df 10 14 Multiple Range Tests for RS by nt Method: 95.0 percent LSD nt Count Mean Homogeneous Groups M-THO 15.9267 X X M-10P-5K 27.83 X M-10P-6K 32.8433 M-20P-5K M-20P-6K 38.61 54.8867 X X Mean Square 617.666 0.0955 F-Ratio 6467.71 Contrast M-10P-5K - M-10P-6K M-10P-5K - M-20P-5K M-10P-5K - M-20P-6K M-10P-5K - M-THO M-10P-6K - M-20P-5K M-10P-6K - M-20P-6K M-10P-6K - M-THO M-20P-5K - M-20P-6K M-20P-5K - M-THO M-20P-6K - M-THO P-Value 0.0000 Sig * * * * * * * * * * Difference -5.01333 -10.78 -27.0567 11.9033 -5.76667 -22.0433 16.9167 -16.2767 22.6833 38.96 * denotes a statistically significant difference + /- Limits 0.562211 0.562211 0.562211 0.562211 0.562211 0.562211 0.562211 0.562211 0.562211 0.562211 LÝ L CH TRÍCH NGANG H tên: NGUY N TH LÝ 24-08-1988 Ngh An a ch liên l c: Xã Nam L c, huy n Nam QUÁ TRÌNH O 2006 2010: Sinh viên t i 2011 , t nh Ngh An ng 2013: H c viên Cao h c t i i h c Nông Lâm Tp.HCM ng i h c Bách Khoa - i h c Qu c Gia Tp.HCM Q TRÌNH CƠNG TÁC 2010 2012: Nhân viên QA t i công ty Liên Doanh TNHH KFC Vi t Nam 2012 2013: Nhân viên nghiên c u t i trung tâm Nghiên C u Tri n Khai Công Ngh B c X ... hóa in vitro c a tinh b t b p s n d ng h t qua trình x lý Argon- plasma áp su ng II NHI M V VÀ N I DUNG: Nghiên c u q trình bi n tính tinh b t b p, s n d ng h t b ng Argon- plasma nh m t o tinh. .. trúc hóa h m hình thái, màu s c kh n nhi t c a tinh b c sau x lý Argon- plasma áp su ng thơng Nghiên c tiêu hóa in vitro c a tinh b c sau x lý Argon- plasma áp su ng Mơ t m hình thái c a tinh b... hi Nghiên c u s bi n tính s tài nghiên c u c iv c u trúc, tính ch xu t m tài tiêu hóa in vitro c a tinh b t b p s n d ng h t qua trình x lý Argon- plasma sáng t nt áp su ng làm tiêu hóa c a tinh
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