ercises in Functional Analysis

Ngày tải lên: 21/09/2013, 15:11

... N-terminal region of DEC1, including the bHLH domain, interacted with the C-terminal r egion o f BMAL1 in a mammalian two-hybrid assay. Accordingly, a Fig. 5. Analysis of DEC1 mutants for binding to ... estern blot analysis (Fig. 5 ). These r esults indicate that the bHLH region, including His57 and Arg65, is responsible for t he E-box binding. Determination of the region in BMAL1 for...

Ngày tải lên: 19/02/2014, 16:20

... transfected individually or in combination into ldlD cells and immunoprecipitation was carried out with myc antibody using detergent-soluble cell lysates containing the amount of protein indicated. ... mutant proteins were also observed in the cytoplasm, as seen for the wild-type protein. Similar staining patterns were observed in other human cell lines, including hepatoma Hep3B cells a...

Ngày tải lên: 07/03/2014, 00:20

... also affects the binding of galactose at some point in the reaction. Modest changes in K m,ATP are seen in H44Y and R68C. That H44 in uences the binding of both substrates suggests that the binding sites ... change in the enzyme, which creates a functional binding site for galactose. Identifying the nature of this change and the residues involved in transmitting information throu...

Ngày tải lên: 08/03/2014, 02:20

... terpenes are produced in resin ducts [2,3]. Their biosynthesis is induced upon wounding [4–6], indicating their role in the defence against bark beetle infestation. For angiosperms many investigations ... after drying dissolved in 5 m M Tris, pH 6.8, 0.2% (w/v) SDS and 1% glycerol. Protein concentration was determined using the BCA Protein assay kit using BSA as protein standard referen...

Ngày tải lên: 08/03/2014, 23:20

... such as: DNA-binding ferritin- like protein, ferritins and bacterioferritins Putative dpsA gene The Dps protein family includes DNA-binding hemoproteins in several bacteria P3 Medium increase sco6501 ... shows an increased level in the dmdR1 mutant, is a DNA-binding protein with domains typical of the ferritin superfamily. This protein might be involved in a cascade of iron regu- lation...

Ngày tải lên: 16/03/2014, 18:20

... same single binding site, rather that I-AniI appears to contain two binding sites. Keywords: Aspergillus nidulans; homing endonuclease; RNA binding protein; DNA sliding; RNA splicing. Group I ... its binding affinity is not altered by the presence of DNA in the DNA binding site. Interestingly, when 3 n M RNA and 10 n M DNA were added simultaneously to a limiting amount of protein in TK8 .....

Ngày tải lên: 17/03/2014, 10:20

... °Cor 22 °C), by using an alternative expression host {BL21(DE3)[pLysS]} or by including protease inhibi- tors in the solutions used during purification. Kinetic analysis of disease-causing mutations The ... protein and from each other. The main effect seen was in the turnover, k cat , and in the specificity constant, k cat ⁄ K m . Again, this contrasts with disease- causing mutations i...

Ngày tải lên: 23/03/2014, 11:20

... similarity to human uridine kinase, UMPK, and UDPK. Uridine kinases are downstream of UP in the pyrimidine salvage pathway. Homology searches revealed that several uridine kinases exist in the C. ele- gans ... protein (UPP-1) exhibited both uridine and thymidine phos- phorylase activity in vitro. Knockdown of other pyrimidine biosynthesis enzyme homologs, such as uridine monophosphate k...

Ngày tải lên: 30/03/2014, 01:20

Ngày tải lên: 07/09/2013, 13:48