... N-terminal region of DEC1, including thebHLH domain, interacted with the C-terminal r egion o fBMAL1 in a mammalian two-hybrid assay. Accordingly, aFig. 5. Analysis of DEC1 mutants for bindingto ... by DEC1 coincidedwith that for interaction with BMAL1 and binding to theE-boxes, indicating the importance of t his region in DEC1for its suppressive activity against CLOCK/BMAL1-induced transcription.Materials ... Arg65, is responsible for t heE-box binding.Determination of the region in BMAL1 for bindingto DEC1To identify the region in BMAL1 required for the binding toDEC1, w e constructed e xpression...
... transfected individually or in combination into ldlD cells and immunoprecipitation was carried out withmyc antibody using detergent-soluble cell lysates containing the amount of protein indicated. ... protein. Tubulin serves as a loading control. The relative amountof mutant protein was calculated based on the ratio of the band intensities of myc-GALE to tubulin in the western blot and indicated ... measuring theintensity of the individual GALE and tubulin bands in the western blot using Scion Image (Scion Corporation,Frederick, MD, USA).Generation of stable cell lines and BrdUincorporation...
... alsoaffects the binding of galactose at some point in thereaction. Modest changes in Km,ATPare seen in H44Y andR68C. That H44 in uences the binding of both substratessuggests that the binding sites ... change in the enzyme, which creates a functional binding site for galactose. Identifying the natureof this change and the residues involved in transmittinginformation through the protein will ... homoserine kinase, proton transfer is unlikely to play amajor role in the rate-determining step of catalysis.Several of the disease-causing mutationsare not soluble following induction in E....
... terpenesare produced in resin ducts [2,3]. Their biosynthesis isinduced upon wounding [4–6], indicating their role in thedefence against bark beetle infestation. For angiospermsmany investigations ... afterdrying dissolved in 5 mMTris, pH 6.8, 0.2% (w/v) SDS and1% glycerol. Protein concentration was determined usingthe BCA Protein assay kit using BSA as protein standardreference, according ... at 3 mLặmin)1. The compounds of interest weretransferred from GC1 to GC2 from 6.6 min to 7.1 min(a-pinene) and 10.2 min to 10.4 min (b-pinene). The fusedsilica capillary column in GC2 (30%...
... DNA-binding proteinof the ferritin family563 nt20 052 DaContains domains typical of the ferritin-likesuperfamily, such as: DNA-binding ferritin-like protein, ferritins and bacterioferritinsPutative ... shows anincreased level in the dmdR1 mutant, is a DNA-bindingprotein with domains typical of the ferritin superfamily.This protein might be involved in a cascade of iron regu-lation in response ... availableregarding ferritin regulation in Streptomyces species. In addition to the 11 proteins listed in Tables 1 and2, minor changes in other proteins were observed.These proteins may be involved in other...
... °Cor22 °C), by using an alternative expression host{BL21(DE3)[pLysS]} or by including protease inhibi-tors in the solutions used during purification.Kinetic analysis of disease-causing mutationsThe ... theseenzymes and inserted into the corresponding sites in theexpression vector pET-21d (Merck, Nottingham, UK). Theentire GALE coding sequence was verified by DNA sequen-cing.The recombinant plasmid ... stained withCoomassie blue. C, control lanes containing 50 lM GALE; P, GALEdigested with 90 nM thermolysin for 30 min at 37 °C; U, GALEdigested with 90 nM thermolysin for 30 min at 37 °C in...
... similarity to human uridine kinase, UMPK, andUDPK. Uridine kinases are downstream of UP in thepyrimidine salvage pathway. Homology searchesrevealed that several uridine kinases exist in the C. ele-gans ... is expressed in the hypo-dermis and intestine, both UP ⁄ UMPK in the hypo-dermis and OPRT ⁄ UMPK in the intestine are essentialfor mediating 5-FU function in C. elegans. It is alsopossible ... acontrol. In vitro enzymatic assaysTo induce the MBP–UPP1 fusion protein, 0.3 lm IPTGwas added to the culture. Induced fusion proteins werepurified using amylose resin (New England Biolabs),according...
... and there exist nonlinear terms in theobjective function and constraints, then (1.1) is a mixed-integer nonlinear programming MINLPproblem. The fundamentals of MINLP optimization ... multipliers in a constrained nonlinear optimization problem have a similar inter-pretation to the dual variables or shadow prices in linear programming. To provide such aninterpretation, ... stationary points of the Lagrangefunction correspond to the minimum of (3.3).Illustration 3.2.2 Consider the following two-variable constrained nonlinear programming prob-lem in the...
... )andt01(1(s))ds =, a(t), a(t) are defined as in Theorem 2.1, and a(0) >t0>0. If x Î C(R+, R+) satisfies the following integral inequality containing multipleintegralsφ(x(t)) ≤ a(t)+α1(t)0f ... R+), and a, j, ψ , ω, a,b, J, Y are defined as in Theorem 2.3. If u ẻ C(R+ì R+, R+) satisfies the following inte-gral inequality containing multiple integralsφ(u(x, y)) ≤a(x, y)+β(y)0α(x)0[f ... R+), i =1,2,anda, j, ψ, ω, J, Yare defined as in Theorem 2.3. If u ẻ C(R+ì R+, R+) satisfies the following integralinequality containing multiple integralsφ(u(x, y)) ≤ a(x, y)+β(y)0α(x)0[f1(s,...
... 11T99AbstractThe main purpose of this paper is to show that many inequalities in functional analysis, probability theory and combinatorics are immediate corollaries of the bestapproximation theorem in inner ... of combinatorics 17 (2010), #R58 4 Proof. Since the total number s of (n − 1)-dimensional hyperplanes passing through theorigin equals the total number of lines passing through the origin,s ... electronic journal of combinatorics 17 (2010), #R58 3 The main purpose of this paper is to show that many inequalities infunctional analysis, probability theory and combinatorics are immediate...
... REV paralog potentially having beenlost during ancestral genomic rearrangements [42], andresulting in a gain of function for the remaining REVsequence in developing xylem and leaves. Ko et ... at 42C in a solution containing 5ì SSC, 0.1% SDS, 0.02%BSA (w/v), 0.01% herring sperm DNA (w/v), and 50%formamide. The slides were then washed twice in 0.1ìSSC, once in water, rinsed in 2-propanol, ... protein 0.000 0.390 -0.803 0.446CN520368 POPTR_0008s06940 E-41 **Cys-3-His zinc finger protein 4.086 -0.332 -0.044 * 0.619CN520805 POPTR_0009s02400 E-93 *leucine rich protein **Brassinosteroid insensitve1-associated...
... -30 in the 5'UTR,(ACAAAAAAGG) position 1083 in the second intron, and(TCAAAAAAGG) position 3052 in intron 5. We detected asingle potential LEAFY binding site (CCATTGT) in thesecond intron ... among the six individuals sequenced, includingall coding and non-coding regions. To determine if therewas any variation that was obscured in a heterozygous state in the direct sequencing, we cloned ... (TCAT-ATTTGG) is located in the second intron at position 785.Similarly. LEAFY binding sites have been determined in intronic regions in Arabidopsis B class genes [34,35]. Thespinach SpAP3 sequences...