Biochemical characterization of thermophilic lignocellulose degrading enzymes and their potential for biomass bioprocessing

Ngày tải lên: 05/09/2013, 16:11

... structure of RNase A tandem enzymes and their interaction with the cytosolic ribonuclease inhibitor Ulrich Arnold, Franziska Leich*, Piotr Neumann, Hauke Lilie and Renate Ulbrich-Hofmann Department of ... crystal structures of three tandem enzymes with the linker sequences GPPG, SGSGSG, and SGRSGRSG, which allowed us to analyze the mode of binding of RI to the RNase A t...

Ngày tải lên: 22/03/2014, 16:21

... with a higher concentration of PGAM than of PPDK at pH 6. The control analysis showed C J Ei values of 0.08 and 0.2 for PGAM and 0.85 and 0.57 for PPDK at 10 and 39 mU of added PPDK, respectively. ... instead of PPi-PFK and PYK instead of PPDK). At pH 6.0, the V max values of amoebal PGAM and PPDK showed a slight increase, those of HK, HPI, TPI and ENO were r...

Ngày tải lên: 07/03/2014, 17:20

... software swiss-pdbviewer and visual molecular dynamics [49]. Acknowledgements We thank G. Magni and S. Ruggieri for helpful com- ments and suggestions and T. Begley (Department of Chemistry and ... in media containing isoform I. Both isoforms were also expressed intracellularly, as evidenced by the appearance of protein bands of the expected size upon SDS ⁄ PAGE analysis of...

Ngày tải lên: 19/02/2014, 02:20

... jellyroll domain formed by two b-sheets and a distal domain formed by insertions between the strands of the jellyroll domain (Fig. 3). Antiparallel b-sheets made up of strands CHEF and BIDG pack against ... ionic strength of the solvent environment and ⁄ or presence of small organic compounds (dioxane and MPD) seems to favor formation of dodecahedra over isolated pentamers....

Ngày tải lên: 19/02/2014, 06:20

... crotonyl-CoA, 3-hydroxybutyryl-CoA and 3-methylcrotonyl-CoA were used for the kinetic characterization of AUH. The data are summarized in Table 2. Overexpression of a mutant form of AUH and its activity on (E)-3-MG-1-CoA Mutations ... procedures Production and purification of human AUH in E. coli The production of AUHp40 (precursor form), AUHp32 (mature form), AUHp40A240 V...

Ngày tải lên: 19/02/2014, 07:20

... large number of orthologs (Figs 2,9). This suggests an essential role for FMN despite the low affinity of the enzyme for that cofactor and the apparent absence of a redox process as part of the catalytic cycle. ... homooctamer structure with a relative mass of 309 kDa [29]. In order to check for the potential influence of substrates and cofactors on the quaternary struct...

Ngày tải lên: 19/02/2014, 13:20

... calcium-binding loops of EF-hand proteins, have chemical shifts . 10 p.p.m. for filled loops and chemical shifts of < 8 p.p.m. for empty loops [50]. The chemical shifts of CR I–II, Calb I – II and several ... University of Debrecen, Hungary; 3 Department of Inorganic Chemistry, and 4 Research Group for Antibiotics, Department of Chemistry, University of Debrecen, H...

Ngày tải lên: 22/02/2014, 07:20

... process- ing of ubiquitin and ubiquitin-like proteins. They are responsible for the disassembly of ubiquitin chains, and for the cleavage of mono- and oligomers of this mole- cule, either in precursor form ... Coomassie stained and major bands were excised and eluted in order to perform N-terminal sequencing. Tandem affinity purification and mass spectrometric analysis...

Ngày tải lên: 07/03/2014, 05:20

... structures of isoquinoline alkaloids, because the structures of many P450s are closely correlated with those of their substrates. Fur- ther identification and characterization of other mem- bers of the ... (R,S)-tetrahydroberberine. Amplification of cytochrome P450 cDNA fragments from single-stranded cDNAs of cultured E. californica cells Single-stranded cDNAs were synthesiz...

Ngày tải lên: 07/03/2014, 10:20