chondroitin degrading enzymes and oligosaccharides

... cellulose- and xylan -degrading enzymes from a thermophilic microbial consortium obtained by enrichment of yard waste compost as a source Materials and methods 2.1 Chemicals and reagents All chemicals and ... (filer paper), BWX and PWSD The enzyme kinetic studies for cellulase and xylanase (Km and Vmax) were performed with 1-10 mg/ml of CMC and BWX, respectively [35] The crude cellulase and xylanase were ... albumin as standard [38] All experiments were run in duplicate and standard deviations (SD) were calculated using Microsoft Excel and results were presented as average ± SD Results and discussion...

Ngày tải lên: 05/09/2013, 16:11

... class II ALDO, iPGAM, and the PPi-dependent enzymes PPi-PFK and PPDK, are not found in human cells (Table 1) Gene cloning, overexpression and purication of recombinant glycolytic enzymes The genes ... temperature at which amoebas grow in vitro and in the host, and at optimal pH and at pH values of 6.0 and 7.0 The Vmax values of the His-tagged recombinant enzymes in the forward (glycolytic) direction ... glycolysis E Saavedra et al in which Vf and Vr represent the maximal forward and reverse rates, Ks and Kp are the Michaelis constants for substrate s and product p, and kz is the rst-order rate constant...

Ngày tải lên: 07/03/2014, 17:20

... future, for treatment and prevention of obesity and diabetes and other diseases The investigation of effects of plant extracts on activity of lipid-carbohydrate metabolic enzymes such as lipase, ... activity of the digestion and absorption of dietary fat such as lipase, lipoprotein lipase and enhancement of carnitine acyl transferases activity and fatty acid oxidation enzymes in fat expenditure ... metabolic enzymes and obesity parameters Life sciences 78 (2006) 2797- 2803 [2] Ide T Effect of dietary α-linolenic acid on the activity and gene expression of hepatic fatty acid oxidation enzymes...

Ngày tải lên: 14/03/2014, 10:20

... (residues 51–57), a b-strand (residues 61–63) and a loop region (residues 75–79) of the N-terminal entity, and a-helix I¢ (residues 4¢–12¢), a loop region (residues 13¢–18¢) and the end of a-helix ... substrate] and cytotoxic (IC50 values between 12.9 lm and 40.0 lm with mammalian K-562 cells) All three variants showed thermodynamic stabilities similar to that of monomeric RNase A, and were ... C-terminal RNase A entity, is shown in pale green The N-terminal and C-terminal RNase A entities are shown in ruby and orange, respectively, and the linker is shown in blue As shown in Fig 2A, binding...

Ngày tải lên: 22/03/2014, 16:21

... aminotransferases Purification and phylogenetic analysis of aminotransferases Enzymes that exhibited GOT, GPT, GGT or AGT activity were subjected to purification, and three enzymes (AT1, AT2 and AT3) were purified ... shown that GOT, GPT and AGT activities were derived from the single enzymes AT1, AT2 and AT3, respectively (Fig 1) GGT activity was caused by AT1 and AT2, which exhibited 11 and 60 UÆ(mg purified ... AT2 and AT3 gave single bands of 44, 42 and 45 kDa on SDS ⁄ PAGE, respectively (Fig 2) The N-terminal amino acid sequences of AT1, AT2 and AT3 were determined to be MNLSKRVSHIKPAPT, MYQERLFTPG and...

Ngày tải lên: 29/03/2014, 09:20

... 3-DeazaThDP and its derivatives and 5, triazole analogues and 7, and open-chain analogues and FEBS Journal 276 (2009) 29052916 ê 2009 The Authors Journal compilation ê 2009 FEBS K Agyei-Owusu and F ... cycle of various ThDPdependent enzymes In so doing, very selective and potent inhibitors of the E1 components (E1p and E1b) of pyruvate dehydrogenase complex (PDHc) and the related branched-chain ... (M D H Wood and F J Leeper, unpublished results) PDHc and branched-chain 2-keto acid dehydrogenase complex belong to the highly complex 2-oxoacid dehydrogenase family of enzymes Enzymes in this...

Ngày tải lên: 29/03/2014, 23:20

... John Smith and Claire Simons Cardiff Univeristy Cardiff, UK Carbonic Anhydrase: Its Inhibitors and Activators Edited by Claudiu T Supuran, Andrea Scozzafava and Janet Conway Enzymes and Their ... John Smith and Claire Simons © 2005 by CRC Press TF1766_title 7/22/04 2:53 PM Page CRC Enzyme Inhibitors Series Enzymes and Their Inhibition Drug Development Edited by H John Smith and Claire ... Cataloging-in-Publication Data Enzymes and their inhibition: drug development / edited by H John Smith and Claire Simons p.; cm — (Enzyme inhibitor) Includes bibliographical references and index ISBN 0-415-33402-0...

Ngày tải lên: 02/07/2014, 11:20

... Enzymes • Definition: E are proteins that participate in cellular metabolic processes with the ability to enhance the rate of reaction between biomolecules The name and classification of enzymes: ... DNA: identification and cloning the insulin DNA and plasmid DNA are mixed together with ligase enzyme the sticky end bases form hydrogen bonds the ligase joins the DNA backbone and a recomninant ... is a non-protein substance which may be organic, and called a coenzyme • Final enzyme: cofactor + apoenzyme Mechanism of enzyme action • Lock and key model: • Induced model: Factors affecting...

Ngày tải lên: 02/08/2014, 08:21

... aims at providing a more complete and comprehensive understanding of the effects of diacerein/rhein on OA subchondral bone metabolism and cells (osteoblasts and osteoclasts) As bone resorption ... activated the ERK1/2 and p38 pathways (Figures 3a and 3b, respectively), diacerein and rhein both significantly inhibited the phosphorylation levels of ERK1/2 (Figure 3a) and both decreased the ... proliferation/differentiation and diacerein and tion and (b) total cells Raw 264.7 cells were incubated for days with RANKL (100 ng/mL) in the presence or absence of interleukin-1-beta (IL-1β) (100 pg/mL) and diacerein...

Ngày tải lên: 09/08/2014, 10:23

... between enzymes The activities of some of the studied enzymes are significantly positively associated (e.g ldh, and Me, ldh and a-Gpd, Me and a-Gpd, Adh and ldh, etc.) On the other hand, there ... activities of many genes, and has a relatively low heritability (e.g., Brncic and Budnik, 1974; De Oliviera and Cordeiro, 1981; Marinkovic and Tucic, 1981 ) In the G-0, G-1, G-5, and G-10generations ... (e.g Britten and Davidson, 1969; Gillespie and Langley, 1974; Stein and Stein, 1976; McDonald and Ayala, 1978; and so on) The difference in the amount of enzymes might be due to a modification of...

Ngày tải lên: 09/08/2014, 22:22

... study These studies will certainly help us understand the role of dosage and the method of administration 14 15 16 17 18 19 Selenoprotein P and selenium compounds, especially sodium selenite, ... Reference Intakes for Vitamin C, Vitamin E, Selenium and carotenoids Edited by Panel on Dietary Antioxidants and Related Compounds, Interpretation and Uses of Dietary Reference Intakes, Institute ... selenoprotein P decrease in severe inflammatory and septic patients In 1st International FESTEM Congress on Trace Elements and Minerals in Medicine and Biology; May 2001 Venice, Italy: Universita...

Ngày tải lên: 13/08/2014, 03:20

... new career and duly submitting would have been so much harder Furthermore, I would like to mention Jackie Whitford and George – the former made such a great and uncomplicated landlady and the latter ... SpUbp21 and/ or SpUbp22 Introduction Screening Results for the Strains ubp21∆::ura4, ubp22∆::ura4 and ubp21∆::ura4 ubp22∆::ura4 Previous Studies, Homology and Domains of Sp Ubp21 and SpUbp22 Meprin and ... Polη, Polι, Polκ, and Rev7, and has been proposed to have a role in gap-filling during G2-M in S cerevisiae (Waters and Walker, 2006) and to recruit Polζ to stalled replication forks and DSBs (reviewed...

Ngày tải lên: 10/10/2014, 23:13

... before and after being treated with water and FFA Textural properties and composition of CaO impregnated silica supports Textural properties of CeS-x and SBA-15 catalyst prepared at pH = and SBA-15 ... gel-formation via co-precipitation) and Candida antarctica lipase (Lp) as the acid, base and bio-catalyst, respectively The catalytic performance (i.e., activity, stability and reusability) of each type ... of SZS, SZM and SZA catalysts at low angle XRD patterns of SZS, SZM and SZA catalysts at high angle TEM micrographs of SZS, SZM and SZA Evolution of FAME yield produced over SZS, SZM and SZA catalysts...

Ngày tải lên: 10/09/2015, 08:25

... Introduction 1.1 Prostate gland and prostate cancer 1.1.1 The anatomy of the prostate gland 1.1.2 Functions of the prostate gland 1.1.3 The histology and histopathology of the prostate gland 1.1.3.1 Normal ... (GlcNAcT-II) coded by EXTL1 and EXTL3 and the heparan sulphate polymerases (GlcA/GlcNAc transferases) coded by EXT1 and EXT2, which are bifunctional enzymes that have both GlcNAcT-II and glucuronyltransferase ... of GlcA and GlcNAc N-deacetylation and N-sulphation of GlcNAc units N-deacetylation and N-sulphation of GlcNAc units N-deacetylation and N-sulphation of GlcNAc units N-deacetylation and N-sulphation...

Ngày tải lên: 12/09/2015, 11:08

... for his guidance and support I would like to thank everybody at the lab for their advice and friendship It’s the best lab I have been in and everybody has been great They are past and present Guang ... being a good friend and helping me ever so often Xue Li for her expert assistance and comments Sravan for his advice and suggestions I am also indebted to Dr Robert Yang and all his lab members ... was recorded under both positive and negative ESI modes with EMS scan type, and ESI conditions are: Turbo Spray source voltage, 5000 and 4500 volts for positive and negative, respectively; source...

Ngày tải lên: 04/10/2015, 16:03

... Nieduszynski IA, Anderson K, Boocock J, Sandall PL & Weeks SD (2001) Characterization of oligosaccharides from the chondroitin sulfates 1H-NMR and 13C-NMR studies of reduced disaccharides and tetrasaccharides ... derived from DS and those derived from CS We have previously reported 1H-NMR data for disaccharides and tetrasaccharides from CS ⁄ DS [16], and Sugahara et al [21] have examined, by 1H NMR and MS, a ... Results and Discussion Isolation of oligosaccharides After depolymerization by chondroitin ABC endolyase, oligosaccharides are isolated as disaccharides, trisaccharides, tetrasaccharides and, because...

Ngày tải lên: 16/03/2014, 14:20

... amino acid transporter-2, and NOS in activated murine microglial cells (19) and co-induction of inducible NOS and arginine recycling enzymes in cytokine-stimulated PC12 cells and high output production ... incidents involving anesthesia To understand the role of citrulline-NO cycle enzymes, GS and the oxidative status in anoxia and reperfusion, NOS, AS, AL, GS and arginase activities along with the ... were removed and killed by decapitation The brains were quickly removed and the different regions (CC, CB and BS) were separated according to the procedure described by Sadasivudu and Lajtha (22)...

Ngày tải lên: 26/10/2012, 09:07

... cells (Kunz and Rudloff, 1993) Sialylated oligosaccharides prevent the binding of E.coli strains associated with neonatal meningitis and sepsis (Kunz and Rudloff, 1993) 697 Human milk oligosaccharides: ... Fierfort and coworkers (2008) and Drounilard and coworkers (2010) have successfully developed a microbiological process to economically produce 3′sialyllactose (Fierfort and Samain, 2008) and 6′sialyllactose ... (Guarner and Malagelada, 2003) In contrast, the infant GIT is more variable in its composition and less stable over time The foetal GIT is sterile and bathed in swallowed amniotic fluid and rapidly...

Ngày tải lên: 28/08/2013, 16:28

... but some code for tetracycline -degrading enzymes (Yang et al., 2004; Nonaka and Suzuki, 2007; Park and Choung, 2007) Degradation of tetracycline by some fungal enzymes was also recently reported ... samples (1, 5, and 10 mg/L), all deionized water samples, and Unoke River water samples (5 and 10 mg/L), after d of incubation However, the density decreased in all rain water samples and the Unoke ... treatment plant effluent (10 mg/L), and rain water (5 and 10 mg/L) More than 20% degradation was observed in all Lake Tedori water and Unoke River water samples and rain water sample (1 mg/L) 100...

Ngày tải lên: 05/09/2013, 10:15

... insulin and key hepatic enzymes in experimental diabetes, Pharmaceutical Biology 40(3), (2002) 165 [8] N Brandstrup, JE Kirk, C Bruni, The hexokinase and phosphoglucoisomerase activities of aortic and ... potato was important crops and was grown popularly every where in the country but up to now, there is no study on anti-obesity and hypoglycemic effects on the basis of the key enzymes activity of carbohydrate ... study is to investigate hypolipidemic and hypoglycaemic effects of extract fractions from Ipomoea batatas leaves Materials and methods 2.1 Plant material and preparation of sweet potato leaf extract...

Ngày tải lên: 12/02/2014, 17:20