Lecture Connections 28 | Regulation of Gene Expression

... CHAPTER 28 Regulation of Gene Expression Key topics: – DNA elements that control transcription – Protein factors that control transcription – Lac operon as a model for regulation – Regulation of protein ... classes of promoters – Allows coordinated expression of different sets of genes • Binding other proteins (transcription factors) to promoters – These recognize promoters of specific genes – These ... post-translational modifications – Allows expression of a specific genes in response to signals in the environment Regulation by  Factors Regulation by Transcription Factors (1) Regulation by Transcription...

Lecture AP Biology Chapter 18 Regulation of gene expression

... transforming a zygote Compare oncogenes, proto-oncogenes, and tumor suppresor genes What are the roles of the ras gene and the p53 gene? Chapter 18         Genes can be activated by inducer ... proto-oncogene  Increase activity of each protein molecule produced by gene  Ras gene: stimulates cell cycle (protooncogene) ◦ Mutations of ras occurs in 30% of cancers  p53 gene: tumor-suppresor gene ... these genes can lead to cancer Proto-Oncogene  Gene that stimulates normal cell growth & division Oncogene   Mutation in protooncogene Cancer-causing gene Effects:  Increase product of proto-oncogene...

Báo cáo khoa học: "In vitro dynamics of HIV-1 BF intersubtype recombinants genomic regions involved in the regulation of gene expression" pdf

... of this study was to gain more insight of the consequences of the BF intersubtype recombination phenomenon on the different but functionally related genomic regions LTR and Vpr/Tat, through the ... directly bind to p300 via interaction the C-terminal αhelix of the protein [29] suggesting that Vpr may act by recruiting them to the HIV-1 promoter thus enhancing viral expression Analysis of the ... amplify the proviral genome regions under study: one comprising the viral promoter and part of the Gag coding sequence (positions 128 to 944 in the HXB2 numbering), and the other including the 3'...

Báo cáo khoa học: " In vitro dynamics of HIV-1 BF intersubtype recombinants genomic regions involved in the regulation of gene expression" docx

Báo cáo y học: "Social regulation of gene expression in human leukocytes" pps

... indications of immune activation emerged in the set of over-expressed genes, including proinflammatory cytokines, chemokines, granzymes, proteinases, and receptors for inflammatory mediators ... effects may be shaped by reduced activity of the anti-inflammatory glucocorticoid transcription control pathway and a complementary increase in activity of the pro-inflammatory NF-κB/ Rel pathway These ... verify the portrait of increased inflammatory signaling that emerged from functional genomic analyses, we assayed circulating levels of C-reactive protein (CRP) in blood samples obtained during...

Báo cáo y học: "Genome-wide patterns of carbon and nitrogen regulation of gene expression validate the combined carbon and nitrogen (CN)-signaling hypothesis in plants" pptx

... was the number of genes in Model CN-enhanced (the child list), k was the number of genes in the pathway being analyzed and in the child list, and p was the number of genes in the pathway being ... model includes genes not regulated by carbon, nitrogen and/ or CN The 'C-only' model includes genes regulated only by carbon Finally, the 'N-only' model includes genes regulated only by nitrogen Three ... shown in yeast that genes encoding ribosomal proteins are induced by nitrogen in the presence of carbon; whether this induction by nitrogen requires carbon to be present was not addressed in the yeast...

Báo cáo y học: "Translational regulation of gene expression" docx

... the regulation of translation of maternally inherited mRNA by cytoplasmic polyadenylation in Xenopus oocytes Polyadenylation is required before the mRNA can be translated, and regulation of this ... therefore necessary for oocyte maturation and embryonic development Cytoplasmic polyadenylation depends on phosphorylation of CPEB, a protein that is bound to specific cytoplasmic polyadenylation elements ... property previously described only for cellular mRNAs RNA-binding proteins have key roles in the regulation of nearly every aspect of gene expression They often display a modular architecture, exert...

Báo cáo y học: "RNA interference is not involved in natural antisense mediated regulation of gene expression in mammals" docx

... which could potentially modulate sense mRNA through two distinct modes of action One of the most exciting findings in genome biology in recent years has been the discovery of RNA interference (RNAi), ... Endogenous singlewell expression of( HIF) and its antisense antisense Figure Endogenous single cell expression of TS sense (TS) and its antisense (rTSα) mRNA, as well as HIF sense (HIF) and its antisense ... data in the present investigation, Jen et al [28] pursued a meta-analysis of NAT expression and suggested that RNA degradation by dsRNA formation is not a predominant route of gene regulation in...

Regulation of gene expression by esrrb in embryonic stem cells

... Chip-sequencing assay 3.4.2 Genome-wide mapping of Esrrb binding sites 3.4.3 Distribution of Esrrb binding and gene expression profiling 3.4.4 Functional relevance of the target genes 3.4.4.1 Esrrb binds ... phenotype induced by Esrrb depletion Thus, Nanog is a key downstream target of Esrrb in maintaining pluripotency In addition, Esrrb is involved in the regulation of genes encoding for chromatin modifiers, ... 3.22 Esrrb binds to its encoding gene in ES cells 105 Figure 3.23 Esrrb binds to the promoter and intronic regions of the Sall4 gene in ES cells 106 Figure 3.24 Esrrb binds to Oct4 gene in ES cells...

Báo cáo khoa học: Improvement of a monopartite ecdysone receptor gene switch and demonstration of its utility in regulation of transgene expression in plants pdf

... (forward, 5¢-AAG GCT TAC CAC GAG CAG CTA TCA-3¢; reverse, 5¢-ACA GGC CAT GTA CTT TCC GTG TCT-3¢) and tobacco (forward, 5¢-ATG AGA GAG TGC ATA TCG AT-3¢; reverse, 5¢-TTC ACT GAA GGT GTT GAA-3¢) a- tubulinspeciﬁc ... VGCfEVY switch in regulating the expression of a zinc ﬁnger protein transcription factor isolated from Arabidopsis thaliana (AtZFP11) in both Arabidopsis and tobacco plants Results Selection of CfEcR ... used in several inducible gene regulation systems to control transgene expression in mammalian cells, transgenic animals, and plants [39] The EcR gene switches described to date are mostly in monopartite...

Analysis of gene expression

... Labeling of a PCR-ampliﬁed probe with DIG as part of in situ RT-PCR indirect detection of gene expression expression standard RT-PCR was performed showing speciﬁc ampliﬁcation of the TNF gene For ... scintillation counting of isolated products on sections of the ﬁlter Virtual Northern blotting Semi-quantitative analysis of gene expression proﬁles, either by Northern blot analysis or by differential ... success of both the DNA elution and re-ampliﬁcation depends on the amount of cDNA generated during the ﬁrst round of PCR A good way of optimizing the ﬁrst round PCR is to take advantage of known genes...

Tài liệu Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression doc

... of gene expression As each step of the RNA metabolism is tightly regulated, the regulation of mRNA export, stability and translation rate is essential for the control of the expression of mRNAs ... localized in the UTRs of the transcript and the trans-acting factors (RNA-binding proteins and noncoding regulatory RNAs) that bind to these sequences Among the best studied regulatory sequences, the ... of the reporter protein fused with the full-length ASF ⁄ SF2 protein, or with the RRM1 alone Most likely, this difference is partly a result of the absence of the RS domain that contributes to...

Báo cáo khoa học: Modiﬁed PCR methods for 3¢ end ampliﬁcation from serial analysis of gene expression (SAGE) tags doc

... nested PCR principle can easily produce 3¢- end cDNA tag-speciﬁc frag- Methods for 3¢ end ampliﬁcation from SAGE tags ments from the SAGE tags (see the protocol in Scheme in the Appendix for more ... (1999) Serial analysis of gene expression: rapid RT -PCR analysis of unknown SAGE tags Nucleic Acids Res 27, e17 34 Chen JJ, Rowley JD & Wang SM (2000) Generation of longer cDNA fragments from serial ... serial analysis of gene expression tags for gene identiﬁcation Proc Natl Acad Sci USA 97, 349–353 35 Richards M, Tan SP, Chan WK & Bongso A (2006) Reverse serial analysis of gene expression (SAGE)...

Báo cáo khoa học: Semi-nested PCR analysis of unknown tags on serial analysis of gene expression potx

... expression: rapid RT -PCR analysis of unknown SAGE tags Nucleic Acids Res 27, e17 Chen JJ, Rowley JD & Wang SM (2000) Generation of longer cDNA fragments from serial analysis of gene expression tags ... for gene identiﬁcation Proc Natl Acad Sci USA 97, 349–353 Richards M, Tan SP, Chan WK & Bongso A (2006) Reverse serial analysis of gene expression (SAGE) characterization of orphan SAGE tags ... al (1999) Analysis of human transcriptomes Nat Genet 23, 387–388 Hashimoto S, Suzuki T, Dong HY, Nagai S, Yamazaki N & Matsushima K (1999) Serial analysis of gene expression in human monocyte-derived...

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