Lecture Connections 28 | Regulation of Gene Expression

... CHAPTER 28 Regulation of Gene Expression Key topics: – DNA elements that control transcription – Protein factors that control transcription – Lac operon as a model for regulation – Regulation of protein ... classes of promoters – Allows coordinated expression of different sets of genes • Binding other proteins (transcription factors) to promoters – These recognize prom...

Ngày tải lên: 15/06/2017, 20:50

... transforming a zygote Compare oncogenes, proto-oncogenes, and tumor suppresor genes What are the roles of the ras gene and the p53 gene? Chapter 18         Genes can be activated by inducer ... proto-oncogene  Increase activity of each protein molecule produced by gene  Ras gene: stimulates cell cycle (protooncogene) ◦ Mutations of ras occurs in 30% of cancers  p5...

Ngày tải lên: 18/05/2017, 08:54

... of this study was to gain more insight of the consequences of the BF intersubtype recombination phenomenon on the different but functionally related genomic regions LTR and Vpr/Tat, through the ... directly bind to p300 via interaction the C-terminal αhelix of the protein [29] suggesting that Vpr may act by recruiting them to the HIV-1 promoter thus enhancing v...

Ngày tải lên: 12/08/2014, 04:21

... of this study was to gain more insight of the consequences of the BF intersubtype recombination phenomenon on the different but functionally related genomic regions LTR and Vpr/Tat, through the ... directly bind to p300 via interaction the C-terminal αhelix of the protein [29] suggesting that Vpr may act by recruiting them to the HIV-1 promoter thus enhancing v...

Ngày tải lên: 12/08/2014, 04:22

... indications of immune activation emerged in the set of over-expressed genes, including proinflammatory cytokines, chemokines, granzymes, proteinases, and receptors for inflammatory mediators ... effects may be shaped by reduced activity of the anti-inflammatory glucocorticoid transcription control pathway and a complementary increase in activity of the pro-inflammatory NF-κB/ Rel...

Ngày tải lên: 14/08/2014, 08:20

... was the number of genes in Model CN-enhanced (the child list), k was the number of genes in the pathway being analyzed and in the child list, and p was the number of genes in the pathway being ... model includes genes not regulated by carbon, nitrogen and/ or CN The 'C-only' model includes genes regulated only by carbon Finally, the 'N-only' model incl...

Ngày tải lên: 14/08/2014, 14:21

... the regulation of translation of maternally inherited mRNA by cytoplasmic polyadenylation in Xenopus oocytes Polyadenylation is required before the mRNA can be translated, and regulation of this ... therefore necessary for oocyte maturation and embryonic development Cytoplasmic polyadenylation depends on phosphorylation of CPEB, a protein that is bound to specific cytoplasmic poly...

Ngày tải lên: 14/08/2014, 14:21

... which could potentially modulate sense mRNA through two distinct modes of action One of the most exciting findings in genome biology in recent years has been the discovery of RNA interference (RNAi), ... Endogenous singlewell expression of( HIF) and its antisense antisense Figure Endogenous single cell expression of TS sense (TS) and its antisense (rTSα) mRNA, as well a...

Ngày tải lên: 14/08/2014, 16:21

... Chip-sequencing assay 3.4.2 Genome-wide mapping of Esrrb binding sites 3.4.3 Distribution of Esrrb binding and gene expression profiling 3.4.4 Functional relevance of the target genes 3.4.4.1 Esrrb binds ... phenotype induced by Esrrb depletion Thus, Nanog is a key downstream target of Esrrb in maintaining pluripotency In addition, Esrrb is involved in the reg...

Ngày tải lên: 12/09/2015, 08:19

... (forward, 5¢-AAG GCT TAC CAC GAG CAG CTA TCA-3¢; reverse, 5¢-ACA GGC CAT GTA CTT TCC GTG TCT-3¢) and tobacco (forward, 5¢-ATG AGA GAG TGC ATA TCG AT-3¢; reverse, 5¢-TTC ACT GAA GGT GTT GAA-3¢) a- tubulinspecific ... VGCfEVY switch in regulating the expression of a zinc finger protein transcription factor isolated from Arabidopsis thaliana (AtZFP11) in both Arabidopsis and tobac...

Ngày tải lên: 16/03/2014, 06:20

... Labeling of a PCR-amplified probe with DIG as part of in situ RT-PCR indirect detection of gene expression expression standard RT-PCR was performed showing specific amplification of the TNF gene For ... scintillation counting of isolated products on sections of the filter Virtual Northern blotting Semi-quantitative analysis of gene expression profiles, either by Northern...

Ngày tải lên: 25/10/2013, 22:20

... of gene expression As each step of the RNA metabolism is tightly regulated, the regulation of mRNA export, stability and translation rate is essential for the control of the expression of mRNAs ... localized in the UTRs of the transcript and the trans-acting factors (RNA-binding proteins and noncoding regulatory RNAs) that bind to these sequences Am...

Ngày tải lên: 16/02/2014, 15:20

... nested PCR principle can easily produce 3¢- end cDNA tag-specific frag- Methods for 3¢ end amplification from SAGE tags ments from the SAGE tags (see the protocol in Scheme in the Appendix for more ... (1999) Serial analysis of gene expression: rapid RT -PCR analysis of unknown SAGE tags Nucleic Acids Res 27, e17 34 Chen JJ, Rowley JD & Wang SM (2000) Ge...

Ngày tải lên: 07/03/2014, 00:20

... expression: rapid RT -PCR analysis of unknown SAGE tags Nucleic Acids Res 27, e17 Chen JJ, Rowley JD & Wang SM (2000) Generation of longer cDNA fragments from serial analysis of gene expression tags ... for gene identification Proc Natl Acad Sci USA 97, 349–353 Richards M, Tan SP, Chan WK & Bongso A (2006) Reverse serial analysis of gene expression (SAGE) ch...

Ngày tải lên: 07/03/2014, 04:20