redox regulation of gene expression and cell function

... Inhibition of uPAR by DNAzymes all of which contribute to focal proteolysis and regulation of wound healing, angiogenesis, and metastasis [5] Of the components of the uPA system, uPAR is of particular ... control and mutant DNAzyme-treated cells (Fig 5) Inhibition of Saos-2 cell invasion by Dz720 The plasminogen activator system can promote the breakdown of ECM and facilitate cell invasion and metastasis ... substrate, and a maximum reduction of 84% of uPAR substrate concentration at a molar ratio of : 200 (Fig 2D) The specificity of the DNAzymes was confirmed by the lack of cleavage by the untreated and...

Ngày tải lên: 16/03/2014, 22:20

... First, single strands of the promoter sequences of the A thaliana genes were randomized 200 times, the reverse complement of the randomized strand was determined, and the number of times the 33 ... dependent and independent component to carbon and nitrogen regulation Two examples of Model are shown (genes Y and Z) For gene Y, nitrogen only has an effect in the presence of carbon, while for gene ... CN-element gene for an alignment Despite this lack of similarity, we tested for the presence of CN1 and CN2 in the promoter of this gene; three copies of CN1 (p-value = 0.052) and one copy of CN2...

Ngày tải lên: 14/08/2014, 14:21

... concomitant increased expression of TauT (Fig 7B) and most probably as a result of restoration of normal cellular taurin level Ó FEBS 2004 TauT expression, activity and subcellular localization ... Figs and the active taurine uptake correlates with TauT expression In order to confirm changes in subcellular level of TauT expression and TauT localization upon supplementation of taurine and ... procedure and the influx experiment we used isotonic standard NaCl medium for control cells and cells pretreated with mM taurine, and standard NaCl medium supplemented with 100 mM sucrose for cells...

Ngày tải lên: 30/03/2014, 15:20

... in situ effect of licofelone on the gene expression and protein synthesis of the major collagenolytic enzymes (MMP-13 and cathepsin K) and aggrecandegrading proteases (ADAMTS-4 and ADAMTS-5) in ... exists between the mRNA and protein levels At the two dosages tested, the R1094 ADAMTS-4 and ADAMTS-5 gene expression and protein synthesis The level of gene expression of ADAMTS-5 in OA cartilage ... The results of a number of studies indicate that the regulatory mechanisms of ADAMTS-4 and ADAMTS-5 gene expression and protein synthesis are complex and may vary based on species and culture...

Ngày tải lên: 09/08/2014, 06:23

... infections, × 106 MT2 cells were infected with B or F and B+F viruses separately at a multiplicity of infection (MOI) of 0.01 IU /cell B and F were mixed before cells addition, and then incubated ... (CATATAA), and E-Box (USF-binding site) A high degree of conservation was observed in all of them but in NFκB and Sp1 sites The HIV-1 promoter contains Sp1 and NF-kB binding sites that regulate gene expression ... 27.3% and 22.7% of the sequences harbored non-parental nucleotide changes at Sp1 II and Sp1III binding sites, respectively, changing to 62.5% and 37.5% at day 10, and to 59% and 40.9% at day 18 of...

Ngày tải lên: 12/08/2014, 04:21

... infections, × 106 MT2 cells were infected with B or F and B+F viruses separately at a multiplicity of infection (MOI) of 0.01 IU /cell B and F were mixed before cells addition, and then incubated ... (CATATAA), and E-Box (USF-binding site) A high degree of conservation was observed in all of them but in NFκB and Sp1 sites The HIV-1 promoter contains Sp1 and NF-kB binding sites that regulate gene expression ... 27.3% and 22.7% of the sequences harbored non-parental nucleotide changes at Sp1 II and Sp1III binding sites, respectively, changing to 62.5% and 37.5% at day 10, and to 59% and 40.9% at day 18 of...

Ngày tải lên: 12/08/2014, 04:22

... immortalized and required IL-2 and stimulation with PHA and feeder cells at 14-day intervals for continued growth MT2 cells harbor 18 integrated proviruses per cell [24], and its level of tax expression ... effects of in vitro and in vivo infections on the cell cycle The percentages of MT2 cells in the G0G1, G2M and S phases of the cycle were respectively 89%, 3%, and 8% For all clones, the cell cycle ... [35], and integration site position [36,37] Given the cell- associated replication of HTLV-1, Tax expression appears ambivalent for infected cells On the one hand it promotes cell cycling and cell...

Ngày tải lên: 12/08/2014, 23:23

... antagonists, and decreased activity of GR transcription cofactors [37] In both human and animal models, prolonged stress has been linked to reduced cellular expression of NR3C1 and increased cellular ... signaling, and prostaglandin synthesis Against this generalized backdrop of immunological activation, however, several functionally distinct subsets of immune response genes showed selective under -expression, ... isolation (denoted x) and other demographic, psychological, or medical covariates (denoted c) that might influence gene expression In these analyses, the profile of expression of each gene across participants...

Ngày tải lên: 14/08/2014, 08:20

... influence of both regulatory proteins and RNA structures Regulation of mRNA utilization by cis elements and trans-acting factors The expression of many proteins is influenced by the structure and cellular ... only for cellular mRNAs RNA-binding proteins have key roles in the regulation of nearly every aspect of gene expression They often display a modular architecture, exert multiple functions and participate ... well as the intricacies of their RNA targets One of the best characterized models of translational control involving cis-acting RNA elements is the regulation of translation of maternally inherited...

Ngày tải lên: 14/08/2014, 14:21

... mRNA, as cell as HIF sense TS sense (TS) and its (aHIF) RNA Endogenous singlewell expression of( HIF) and its antisense antisense Figure Endogenous single cell expression of TS sense (TS) and its ... is a translation block or other kind of RNA mediated regulation of gene expression, without alteration of mRNA levels Expression assessment and evaluation of mRNA levels would be recommended as ... comparing the expression of TS, rTSα, HIF and aHIF with that of a relatively low abundance gene product, TATA binding protein (TBP) The S-AS mRNA expression levels were 5% to 13% of that of β2M, as...

Ngày tải lên: 14/08/2014, 16:21

... approaches to understand ES cell biology 1.3.1 Alteration of gene expression by genetic perturbation 1.3.2 Genomic approaches to study gene expression in ES cells 1.4 Objective and value of this project ... mapping of Esrrb binding sites 3.4.3 Distribution of Esrrb binding and gene expression profiling 3.4.4 Functional relevance of the target genes 3.4.4.1 Esrrb binds to ES cell- associated genes 3.4.4.2 ... the study of ES cell biology First of all, mouse ES cells provide a universal cell source for the study of embryogenesis, gene function and directed differentiation Because mouse ES cells are...

Ngày tải lên: 12/09/2015, 08:19

... distribution of glutathione peroxidases and peroxiredoxins throughout the cell 1.2 REDOX REGULATION OF GENE EXPRESSION The regulation of gene expression is the control of the level and timing of appearance ... RESULTS – REDOX REGULATION OF ERα 58 3A.1 H2O2 AND THE EXPRESSION OF ERα PROTEIN 58 3A.1.1 Morphology and growth of MCF7 cells upon exposure to H2O2 58 3A.1.2 Effect of H2O2 on the expression ... of ERα as a redox regulated gene 172 4.1.4 ROS-induced suppression of ERα gene expression: Possible development of ERα-negative breast cancer phenotype 178 viii 4.2 REDOX REGULATION OF...

Ngày tải lên: 10/09/2015, 15:49

... c-myc gene expression To investigate whether NAMI-A-induced inhibition of ERK1/2 signaling could affect the expression of a cell growth-related gene, we assessed c-myc gene expression in growing cells ... between the inhibitory effect of NAMI-A on ERK1/2 activation and NAMI-A- induced down regulation of c-myc gene expression NAMI-A inhibits c-myc gene transcription and protein expression To establish ... endothelial cell plays a crucial role in angiogenesis [39] and that it might affect the growth and differentiation of neighboring cells [40] The endothelial cell is also the target cell for tumor...

Ngày tải lên: 21/02/2014, 01:21

... important aspects of the gene switch together and develop a tight EcR gene regulation system with improved ligand sensitivity and reduced background expression in the absence of chemical ligand [15] Our ... severely deformed and dwarfed and did not set seed (data not shown) Molecular genetic approaches such as antisense RNA, loss of function, gain of function, ectopic expression and overexpression cannot ... of methoxyfenozide The two-hybrid gene regulation system requires two receptor gene expression cassettes (DBD:EcR and AD:RXR), whereas the monopartite gene switch requires one receptor gene expression...

Ngày tải lên: 16/03/2014, 06:20

... the regulation of cell function both comprehensively and fairly quantitatively Yet it is of crucial importance to evaluate how much of a given regulation is effected at the level of gene expression ... due to regulation at the activity level and 28% (2.2 of 7.8) is due to regulation at the gene expression level Of the latter 28%, 7% is accounted for by regulation through topoisomerase I expression ... of gyrase activity and gyrase expression for supercoiling are negative (i.e the activity and expression of DNA gyrase is inhibited not stimulated by higher levels of supercoiling) and those of...

Ngày tải lên: 18/03/2014, 01:20

... (lanes 1, and 4) Effect of the )1CfiA mutation on reporter gene expression To further confirm that the )1CfiA mutation is associated with low expression of the b2GPI gene, we performed Fig Effect of the ... [30,31] However, our in vitro mutagenesis and expression study did not link these mutations to an altered b2GPI gene expression [32] Although, in vitro mutagenesis and expression study not rule out ... 4.3 and 7.3 mgÆdL)1) This indicates that the genetic basis of APA is complex and other genetic and/ or biological factors are involved in the occurrence of APA The structural organization of the...

Ngày tải lên: 31/03/2014, 07:20

... FGF-2 regulation and sequential expression of growth, angiogenic and chemotactic genes and depresses expression of mineralization-related genes, these figures not tell us the relative abundance of ... induction of proliferation and angiogenesis; qRTPCR analysis of gene reduction of genes over 24 hours of treatment with FGF-2 shows a significant increase in genes associated with proliferation and ... hour of each gene) Relative mineralization of FGF-2 and BMP-2 treated cells As seen in Figure and Table 3, BMP-2 treatment enhances mineralization of the cells as shown by uptake and presence of...

Ngày tải lên: 20/06/2014, 04:20

... FGF-2 regulation and sequential expression of growth, angiogenic and chemotactic genes and depresses expression of mineralization-related genes, these figures not tell us the relative abundance of ... induction of proliferation and angiogenesis; qRTPCR analysis of gene reduction of genes over 24 hours of treatment with FGF-2 shows a significant increase in genes associated with proliferation and ... hour of each gene) Relative mineralization of FGF-2 and BMP-2 treated cells As seen in Figure and Table 3, BMP-2 treatment enhances mineralization of the cells as shown by uptake and presence of...

Ngày tải lên: 20/06/2014, 07:20

... Memory B cells 60% BBcells cells B cells CD27 Expression CD27 Expression Plasmablasts Naïve Naïve B cells B cells Memory Memory B cells B cells CD27 Expression Schematic distribution of B cell subsets ... peripheral naive (IgM+/CD27–) and memory (CD27+) B cells Interaction of CD27 with its ligand on T cells, CD70, serves as a pathway of differentiation of B cells into plasma cells [73,75,76] Recently, ... regions of the VH molecule Evidence of clonal expansions The major salivary glands are known to be the site of preferential B cell expansions and in some cases of lymphoproliferation in pSS B cells...

Ngày tải lên: 09/08/2014, 01:21

... targeting of these complexes Consequently, an oxidant-mediated reduction in the protein expression of Mi-2α and mSin3a and increased protein expression of Mi2β may lead to altered gene repression ... understanding of the roles of these co-repressor complexes and thereafter their roles in disease In summary cigarette smoke exposure reduced HDAC2 activity and the expression of mSin3a and Mi-2α ... Transcription Cell 1999, 99:443-446 Denslow SA, Wade PA: The human Mi-2//NuRD complex and gene regulation Oncogene 2007, 26:5433-5438 Silverstein RA, Ekwall K: Sin3: a flexible regulator of global gene expression...

Ngày tải lên: 11/08/2014, 03:20