androgen regulation of gene expression

... delicate mechanism of regulation of viral gene expression by compensating any losses or gains in transcription levels This mechanism may play a key role in the adaptation process of recombinant variants ... including transactivation of viral [16] and host cell genes, regulation of the reverse-transcription process accuracy, viral DNA nuclear import, cell cycle progression, and apoptosis regulation http://www.virologyj.com/content/6/1/107 ... degree of conservation was observed in all of them but in NFκB and Sp1 sites The HIV-1 promoter contains Sp1 and NF-kB binding sites that regulate gene expression via the recruitment of both...

Ngày tải lên: 12/08/2014, 04:21

... delicate mechanism of regulation of viral gene expression by compensating any losses or gains in transcription levels This mechanism may play a key role in the adaptation process of recombinant variants ... including transactivation of viral [16] and host cell genes, regulation of the reverse-transcription process accuracy, viral DNA nuclear import, cell cycle progression, and apoptosis regulation http://www.virologyj.com/content/6/1/107 ... degree of conservation was observed in all of them but in NFκB and Sp1 sites The HIV-1 promoter contains Sp1 and NF-kB binding sites that regulate gene expression via the recruitment of both...

Ngày tải lên: 12/08/2014, 04:22

... http://genomebiology.com/2007/8/9/R189 decreased expression of the GR NR3C1 gene, post-translational modification of GR protein, increased expression of GR antagonists, and decreased activity of GR transcription cofactors [37] ... gene expression In these analyses, the profile of expression of each gene across participants (denoted y) was first adjusted to remove any variance attributable to a covariate (based on the general ... prevalence of distinct leukocyte subsets within the mononuclear cell RNA pool [45-47], analyses of covariance (ANCOVA) were employed to adjust gene expression profiles for the relative prevalence of...

Ngày tải lên: 14/08/2014, 08:20

... regulate the expression of genes in Arabidopsis We predicted six general models that could describe the possible modes of gene regulation due to carbon, nitrogen and CN together Three of these models ... qualitatively compare gene- expression profiles across multiple treatments For each gene, each treatment is assigned a value representing the effect of the treatment on the expression of that gene Treat- ... classes represent a broad spectrum of expression patterns that validate each of the six general models for gene regulation This analysis shows that of the 3,652 genes in the analysis, the vast majority...

Ngày tải lên: 14/08/2014, 14:21

... roles in the regulation of nearly every aspect of gene expression They often display a modular architecture, exert multiple functions and participate in more than one step of the gene- expression ... well as the intricacies of their RNA targets One of the best characterized models of translational control involving cis-acting RNA elements is the regulation of translation of maternally inherited ... ribosome The examples of regulation of protein expression by signaldependent translation (or inhibition of translation) presented at the meeting are consistent with the general prediction that...

Ngày tải lên: 14/08/2014, 14:21

... is a translation block or other kind of RNA mediated regulation of gene expression, without alteration of mRNA levels Expression assessment and evaluation of mRNA levels would be recommended as ... the sensitivity of our methods by comparing the expression of TS, rTSα, HIF and aHIF with that of a relatively low abundance gene product, TATA binding protein (TBP) The S-AS mRNA expression levels ... can be ruled out on this criterion alone Expression levels of S-AS pairs are also important, as these could predict the mode of regulation High levels of SAS pairs in a single cell, as suggested...

Ngày tải lên: 14/08/2014, 16:21

... regions of the Sall4 gene in ES cells 106 Figure 3.24 Esrrb binds to Oct4 gene in ES cells 107 Figure 3.25 The regulation of Esrrb on ES cell-associated genes 108 Figure 3.26 Expression profiles of ... region of Sox17 gene in ES cells 122 x Figure 3.31 Esrrb binds to Gata6 gene in ES cells 123 Figure 3.32 The regulation of Esrrb on developmental genes 125 Figure 3.33 Expression profile of Jmjd3 ... mapping of Esrrb binding sites 3.4.3 Distribution of Esrrb binding and gene expression profiling 3.4.4 Functional relevance of the target genes 3.4.4.1 Esrrb binds to ES cell-associated genes 3.4.4.2...

Ngày tải lên: 12/09/2015, 08:19

... Tavva et al A Improvement of EcR gene switch C D B E Fig Schematic representation of the chemical-inducible EcR gene regulation systems Monopartite gene switch: the chimeric gene, AD:DBD:EcR LBD, ... receptor gene expression cassettes (DBD:EcR and AD:RXR), whereas the monopartite gene switch requires one receptor gene expression cassette (AD:DBD:EcR), to transactivate the reporter gene expression ... demonstration of its utility in regulating the expression of transcription factor in plants The ability to tightly regulate gene expression in plants is an essential tool for the elucidation of gene function...

Ngày tải lên: 16/03/2014, 06:20

... Labeling of a PCR-amplified probe with DIG as part of in situ RT-PCR indirect detection of gene expression expression standard RT-PCR was performed showing specific amplification of the TNF gene For ... success of both the DNA elution and re-amplification depends on the amount of cDNA generated during the first round of PCR A good way of optimizing the first round PCR is to take advantage of known genes ... intensity of green versus red This gives a measure of the relative levels of the competing mRNAs bound to each spot and so provides information on the relative level of expression of the genes on...

Ngày tải lên: 25/10/2013, 22:20

... crucial role played by post-transcriptional regulation in the control of gene expression As each step of the RNA metabolism is tightly regulated, the regulation of mRNA export, stability and translation ... activity of both RRMs Finally, the association of ASF ⁄ SF2 with TIAR led us to investigate its role on the expression of reporter gene bearing an ARE in its 3¢ UTR We showed that overexpression of ... might modulate the expression of ARE-containing genes Accordingly, we tested the effect of overexpressing ASF ⁄ SF2 on the expression of Renilla luciferase (Rluc) reporter genes carrying (or...

Ngày tải lên: 16/02/2014, 15:20

... single or multiple gene predictors not involved ‡ in one of the gene expression profile tests of interest: 150 Article does not involve one of the three gene expression tests of interest: 659 No ... later This cut-off year was established based on the introduction date of the MeSH heading gene expression profiling,” 2000, and the introduction date of the MeSH heading gene expression, ” 1990 ... between the investigated gene expression signatures (see Glossary, Appendix B) and the actual gene expressionbased tests The gene “signature” is the collection of genes whose expression levels are...

Ngày tải lên: 06/03/2014, 01:20

... (2005) Analysis of long-lived C elegans daf-2 mutants using serial analysis of gene expression Genome Res 15, 603–615 10 Ryu EJ, Angelastro JM & Greene LA (2005) Analysis of gene expression changes ... number of serial analysis of gene expression tag sequences into 3¢ complementary DNAs Genes Chromosomes Cancer 33, 252–261 33 van den Berg A, van der Leij J & Poppema S (1999) Serial analysis of gene ... gene expression: rapid RT-PCR analysis of unknown SAGE tags Nucleic Acids Res 27, e17 34 Chen JJ, Rowley JD & Wang SM (2000) Generation of longer cDNA fragments from serial analysis of gene expression...

Ngày tải lên: 07/03/2014, 00:20

... of gene expression: rapid RT-PCR analysis of unknown SAGE tags Nucleic Acids Res 27, e17 Chen JJ, Rowley JD & Wang SM (2000) Generation of longer cDNA fragments from serial analysis of gene expression ... known of the function of mRNAs in human spermatozoa, it was difficult to estimate whether the rest of the genes were related to the function of human spermatozoa, or just retained during spermatogenesis ... (2003) An anatomy of normal and malignant gene expression Proc Natl Acad Sci USA 99, 11287–11292 10 Lee S, Zhou G, Clark T, Chen J, Rowley JD & Wang SM (2001) The pattern of gene expression in human...

Ngày tải lên: 07/03/2014, 04:20

... impairment of the NF-jB-mediated regulation of the transcription of TauT From Fig 6C it is seen that the taurine-induced downregulation of TauT is reversible and that TauT in NIH3T3 cells regain 50% of ... up -regulation of TauT mRNA expression in astrocyte primary cultures and they suggested that TauT expression is controlled by a taurine-induced down -regulation and an osmolarity-induced up -regulation ... phosphorylation of the inhibitory complex IjB, dissociation of the heterodimer NF-jB (p50, RelA) from IjB, translocation of NF-jB to the nucleus and subsequent initiation of gene expression HOCl...

Ngày tải lên: 30/03/2014, 15:20

... http://www.translational-medicine.com/content/9/1/44 Page of Figure Sorting of human CD8+ T lymphocytes and microRNA large scale expression profiling a) CD8+ T cells from healthy volunteers were FACS-sorted based on their expression of CD45RA, ... variability, the expression of some of these miRNAs was found to be preferentially associated with specific subsets of CD8+ T cell differentiation Interestingly, the expression level of miR-20a was ... clustering of the expression levels of a selected set of microRNAs in each subset normalized to levels in naive cells (log2 fold change) (green: downregulated/red: upregulated) b) Expression levels of...

Ngày tải lên: 18/06/2014, 19:20

... regarding the potential hierarchy of expression of these three genes The CrLFC showed upregulation of both MMP 13 and COX-2, while the CaMTP showed upregulation of MMP 13 This could indicate that ... content of the tissues between ACL-X and control joints for any of the regions tested Error bars indicate standard error of the mean Values are μg of HP/mg of tissue wet weight Differential gene expression ... feasibility of analyzing changes in gene expression of articular cartilage two weeks after ACL-transection The specific aims of this study were to determine if changes in relevant gene expression...

Ngày tải lên: 20/06/2014, 00:20

... http://www.translational-medicine.com/content/9/1/44 Page of Figure Sorting of human CD8+ T lymphocytes and microRNA large scale expression profiling a) CD8+ T cells from healthy volunteers were FACS-sorted based on their expression of CD45RA, ... variability, the expression of some of these miRNAs was found to be preferentially associated with specific subsets of CD8+ T cell differentiation Interestingly, the expression level of miR-20a was ... clustering of the expression levels of a selected set of microRNAs in each subset normalized to levels in naive cells (log2 fold change) (green: downregulated/red: upregulated) b) Expression levels of...

Ngày tải lên: 20/06/2014, 03:20

... quantification of gene expression was calculated by using 2-(Ct gene T - Ct CPHI T)-(Ct gene hr - Ct CPHI hr) equation, where “Ct gene T” represents the calculated threshold cycle (C t ) of a time point of ... analysis of gene induction of proliferation and angiogenesis; qRTPCR analysis of gene reduction of genes over 24 hours of treatment with FGF-2 shows a significant increase in genes associated with ... mineralization [1] Page of Figure qRTPCR analysis of FGF-2 regulated genes associated with mineralization; qRTPCR analysis of gene reduction of genes over a 24 hours of treatment with FGF-2 shows...

Ngày tải lên: 20/06/2014, 04:20

... quantification of gene expression was calculated by using 2-(Ct gene T - Ct CPHI T)-(Ct gene hr - Ct CPHI hr) equation, where “Ct gene T” represents the calculated threshold cycle (C t ) of a time point of ... analysis of gene induction of proliferation and angiogenesis; qRTPCR analysis of gene reduction of genes over 24 hours of treatment with FGF-2 shows a significant increase in genes associated with ... mineralization [1] Page of Figure qRTPCR analysis of FGF-2 regulated genes associated with mineralization; qRTPCR analysis of gene reduction of genes over a 24 hours of treatment with FGF-2 shows...

Ngày tải lên: 20/06/2014, 07:20

... μxg represents the mean of xg 2.3 Extraction of Shape Information and Time Scaling To extract shape information of time-varying gene expression, the derivative of the expression trajectory is ... optimal number of clusters was arrived at by multiple runs of the algorithm at values of K, 3.7 Validation of E Coli Expression Data Results We validated the results of our tests using Gene Ontology ... vector of time points associated with gene g, zg is the vector of transformed time-series data (from (1)) associated with gene g, and yg is the resulting vector of first differences associated with gene...

Ngày tải lên: 22/06/2014, 00:20