In vitro culture of small floral receptacle of gerbera jamesonil

... Cornu D & Miginiac E (1989) Endogenous levels of abscisic acid, indole-3-acetic acid and benzyladenine during in vitro bud growth induction of wild cherry (Prunus avium L.) Plant Growth Regul ... Epstein and Lavee (1986) reported a transformation of IBA into IAA during in vitro culture of Vitis vinifera and Olea europea The chemical pathway could be a 0...

Ngày tải lên: 09/08/2014, 04:20

... in thin cell layers Int Rev Cytol 32:291–311 Tran Thanh Van K, Trinh NH (1978) Thin cell layers, Concept Methodology and Results In: Thorpe TA (ed) Frontiers of Plant Tissue Culture Int Ass of ... tTCLs of Panax ginseng after nine weeks in MS medium containing 2,4-D (5 M) under 16-hour photoperiod and 100 Mm-2s-1 light intensity In present experiment, petiole lTCL...

Ngày tải lên: 20/04/2015, 21:33

... type of collagen in the ECM This research also focuses on the effect of the suppression of the MMP-3 gene on mRNA and proteoglycan production Moreover, the biological effects of the suppression of ... increased cell viability and mitotis Moreover, a suppression of MMP-3 can increase production of GAG and HA For further study, MMP-3 gene suppr...

Ngày tải lên: 20/06/2014, 04:20

... formation According to Liu and Foote [14] nonessential amino acids (NEAA) have a stimulatory effect upon all developmental stages, and essential amino acids (EAA) enhance blastocyst formation and the ... Non-essential amino acids d Bovine serum albumin (fatty acid free, fraction V) e Fetal bovine serum Ta ble Effect of protein source on the in vitro development...

Ngày tải lên: 07/08/2014, 17:22

... 20 Isolation and characterization of T-DNA flanking sequences Isolation of T-DNA flanks was accomplished with Thermal Asymmetric Interlaced-PCR (TAIL-PCR) and Inverse PCR (I-PCR) TAIL-PCR was ... Sequences were analyzed with BLASTn and BLASTx http://www.ncbi.nlm.nih.gov/BLAST/ programs against the GenBank database, and against a banana EST database donated by Syngent...

Ngày tải lên: 12/08/2014, 03:20

... numbers of samples are involved (Section 5.4) Analysis, sequencing and in vitro expression of PCR products 93 5.4 Direct DNA sequencing of PCR products Once a PCR product has been cloned into a ... primer in excess continues to allow linear accumulation of one strand of DNA suitable for subsequent DNA sequencing Analysis, sequencing and in vitro...

Ngày tải lên: 25/10/2013, 22:20

... of the triangular prism binding pocket, with its amino sugar anchored Expansion of DNA repeat sequences in the major groove of the DNA pointing toward the 3¢ -bulge- flanking base pair We were interested ... as the ligand–nucleic acid complex is formed To gain insight into the binding of drugs to bulge DNA, several bulgecontaining oligonucleotides were selected as binding...

Ngày tải lên: 07/03/2014, 06:20

... particular phase of the cell cycle, and was associated with an increase in the sub-G1 (apoptotic) portion of the cell cycle We next investigated the effect of combining CDO with the chemotherapeutic ... possible model of drug interaction in GEO cells, which involves the complex interaction of proteins involved in cell cycle regulation To recapitulate, com...

Ngày tải lên: 07/03/2014, 15:20

... plot of nuclear NF-jB from in vitro and in silico analysis of the data The plot shows nuclear NF-jB oscillation in the original model and in the updated model with newly measured kr1, ka1 and ... described for the kinase time-course assay The assay was again repeated with the inclusion of 10 mm MnCl2 in the kinase condition In vitro analysi...

Ngày tải lên: 16/03/2014, 11:20

... the only protein with which one can biochemically assay both DNA endonuclease and RNA maturase activities in vitro This investigation provides the first step in the study of the relationship between ... stringent concentration of KCl, there was a correlation between inhibition of endonuclease activity and inhibition of splicing activity indicating that R...

Ngày tải lên: 17/03/2014, 10:20

... Evidence of different steps in the GPI-bALP mediated mineralization: effects of Ca2 + concentration To assess the role of Ca2 + in initiating the mineralization process, we modelled the biological process ... taken and centrifuged Ca2 + and Pi were determined in the pellets corresponding to the mineral structure Symbols corresponding to various Ca2 + concentrations a...

Ngày tải lên: 23/03/2014, 17:21

... the addition of NaOH to a 0.2 M final concentration) The concentration of NaCl was also increased in a separate assay PTOX activity is given as percentage of starting O2 consumption (before addition ... PTOX activity To examine the role of iron and other metals, PTOX expressing bacteria and control bacteria were grown in the Table Effect of citrate, malate and NaCl on PTOX act...

Ngày tải lên: 31/03/2014, 07:20

... loading concentration and solvent composition on the in vitro release of ketoprofen To compare and contrast the in vitro release rates of ketoprofen from proprietary gel products and extemporaneously ... Structure of ketoprofen 36 Stereochemistry of ketoprofen 37 Synthesis of ketoprofen starting from (3-carboxy-phenyl)-2-propionitrile .39 Synthes...

Ngày tải lên: 13/04/2014, 09:25

... factors in the supernatant inhibit the maturation of fusion cells and have a negative impact in the stimulation of T cells To determine the induction of WT1-specitic CD8+ T cells, a pentameric ... supernatants in the generation of Treg in vitro is demonstrated in the present study, little is known about the impact of fusion cell vaccination on generating Tre...

Ngày tải lên: 18/06/2014, 15:20