animal and in vitro models of immunotoxicity

Báo cáo y học: "Argon: Neuroprotection in in vitro models of cerebral ischemia and traumatic brain injury" pdf

Ngày tải lên : 13/08/2014, 20:21

... injury were first incubated for 30 minutes at 37°C, 95% air and 5% CO2 in EM containing 4.5 μM PI Following baseline fluorescence imaging the traumatic brain injury was generated using an apparatus ... two and three hours after injury Discussion We investigated the potential neuroprotective effects of the noble gas argon in two in vitro models of OGD and TBI Our methods involved depriving cultured ... benzodiazepine binding site and possibly at multiple other discrete sites of the GABAA receptor [49] The activation of GABA receptors has been shown to be neuroprotective in in vitro and in vivo...

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Analysis, sequencing and in vitro expression of PCR products

Ngày tải lên : 25/10/2013, 22:20

... a combination of such factors (Chapter 4) In such Analysis, sequencing and in vitro expression of PCR products 89 cases it is often possible to increase the sensitivity of the analysis in order ... separation in thin-coated capillaries containing nonpolymerized gel matrices and laser detection systems The introduction and removal of polymer from the capillaries, plus loading and running samples and ... exhausted the primer in excess continues to allow linear accumulation of one strand of DNA suitable for subsequent DNA sequencing Analysis, sequencing and in vitro expression of PCR products 99...

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báo cáo hóa học: " Expression profiles for macrophage alternative activation genes in AD and in mouse models of AD" pptx

Ngày tải lên : 19/06/2014, 22:20

... profile of macrophages can change, shutting down the production of pro-inflammatory cytokines and increasing the production of factors that participate in tissue repair and wound healing [6] Anti-inflammatory, ... related chitinase genes, namely CHI3L1 and CHI3L2 are overexpressed in AD brain Both forms of chitinase 3-like proteins not have enzymatic chitinase activity and instead, inhibit IL-1 and TNFα-mediated ... Calingasan N, Erdely H, Altar C: Identification of CD40 ligand in Alzheimer's disease and in animal models of Alzheimer's disease and brain injury Neurobiol Aging 2002, 23:31-39 Townsend K, Town T, Mori...

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báo cáo hóa học: " Beyond platinum: synthesis, characterization, and in vitro toxicity of Cu(II)-releasing polymer nanoparticles for potential use as a drug delivery vector" ppt

Ngày tải lên : 21/06/2014, 02:20

... synthesis, and metal loading RP conceived of the study, and participated in its design and coordination, carried out in vitro toxicity studies, SEM and EDX analysis and drafted the manuscript Competing ... of added competing ligand, (D) release as a function of pH in the absence of competing ligand effects observed in ultrapure water with approximately 95% of the loaded Cu remaining bound to the ... difference between the amount released at 48 h and that contained in the original loading solution determined Cu loading, resulting in values ranging between 12 and 16 wt.% based on these reactions conditions...

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Báo cáo lâm nghiệp: "Eﬃcient method of micropropagation and in vitro rooting of teak (Tectona grandis L.) focusing on large-scale industrial plantations" pptx

Ngày tải lên : 07/08/2014, 16:20

... auxins, it promoted early rooting and increased the ﬁnal rooting percentage and the number of roots per explant [21, 22] Putrescine is involved in root induction by increasing the activity of ... the use of putrescine, which in combination with low concentration of auxin, in rooting medium, was essential in stimulating high rooting percentage with high quality of roots, resulting in fast ... reducing vitriﬁcation problems and aimed at improving shoot length and rooting percentage, by modifying the medium composition in terms of salt balance, type of gelling agent, by adding pectin of...

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Báo cáo khoa học: " Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos" pps

Ngày tải lên : 07/08/2014, 20:23

... gniniatnoc pord muidem noitalupinam a otni derrefsnart dna ,muidem noitalupinam hserf ni eciwt dehsaw ,)24333 tshceoH lm/gµ gniniatnoc ASB-HLT eerf-muiclac( muidem noitalupinam ni nim 51 rof ... ,h rof edimixeholcyc lm /gµ 01 gniniatnoc muidem CVI ni detabucni erew setycoo detalumits-ortcelE 2lCgM Mm 50.0 dna 2lCaC Mm 10.0 gniniatnoc lotinnam Mm 082 ni ces yb detarapes cesµ 06 rof V ... )v/w( %4.0 gniniatnoc muidem 32 -)USCN( ytisrevinU etatS aniloraC htroN saw tnempoleved oyrbme rof muidem )CVI( erutluc ehT stnemirepxe lla ni desu saw hctab emas eht morf diulf ralucillof enicroP...

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Báo cáo y học: "Phenotype and in vitro function of mature MDDC generated from cryopreserved PBMC of cancer patients are equivalent to those from healthy donors" ppt

Ngày tải lên : 11/08/2014, 10:23

... repertoire of DC matured in the presence of inflammatory stimuli comprises pro-inflammatory cytokines and chemokines, including the T cell inhibitory cytokine IL-10, the Th-1 promoting cytokine IL-12, ... Mixtures of peptides consisting of 15 amino acid residues, overlapping by 11 amino acids each, were designed to span the sequences of CMV pp65, CEA, MAGE-3, and the intracellular domain (ICD) of HER-2/neu ... PerCP-Cy5.5 and CD4 APC and acquired on a flow cytometer CD3+CD4+ lymphocytes were gated including the blasts and excluding CD209+ MDDC The percentage of cells showing decreased CFSE staining intensity...

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Báo cáo khoa học: "In vivo and in vitro evaluation of an Acetobacter xylinum synthesized microbial cellulose membrane intended for guided tissue repair" ppt

Ngày tải lên : 12/08/2014, 18:22

... Differences in the manufacturing according to initial concentrations of carbon sources, surface/volume ratios, strain of Acetobacter and extended times of fermentation interfere in the final product ... Committee at the School of Veterinary Medicine and Animal Science, São Paulo State University Before surgery, anesthesia was induced by intramuscular injection of a combination of xylazine 2%, 10 mg/kg ... of inflammatory and immune response, means of disintegration and its longevity in the host tissue [1] The inflammatory response may delay the healing process [23] In the present study a low inflammatory...

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In vivo and in vitro studies of th17 response to specific immunotherapy in house dust mite induced allergic rhinitis patients

Ngày tải lên : 23/09/2015, 14:47

... treatment were used in the following in vivo and in vitro studies 3.3 In vitro Study To mimic the functional response of Th17 and other Th cell subpopulations to HDM allergens, an in vitro allergen ... between in vivo and in vitro findings, confirming that not only Th2, but also Th17 cells play an important role in the context of allergic inflammation IL10 producing CD4+ T cells showed a distinct ... markers in each study group tested in vitro 3.2 In vivo Study 3.3.1 Th17 specific gene mRNA/protein expression Following a 3-day in vitro stimulation, there were mRNA/protein levels of Th17 associated...

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Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf

Ngày tải lên : 07/03/2014, 06:20

... (DDIs and doxorubicin) The DNA bulge binding of both compounds was detected by CD and UV melting experiments Possible slippage mechanisms are discussed Results and Discussion Effect of DDI-1A and ... change in Tm (DTm) for the ATT bulge increased by 3.4 and 1.7 °C in the presence of DDI-1A and DDI-1B, respectively The increase in DTm for DDI-1A was higher than that for DDI-1B, implying that intercalation ... binding pocket, with its amino sugar anchored Expansion of DNA repeat sequences in the major groove of the DNA pointing toward the 3¢-bulge-ﬂanking base pair We were interested in small molecules that...

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Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc

Ngày tải lên : 07/03/2014, 21:20

... harvesting complex (LHCI), consisting of four members (Lhca1–4) of the chlorophyll a ⁄ b binding protein family LHCI is bound along one side of PSI only, in the vicinity of the PSI-F and PSI-J ... extreme C-terminus of PSI-G (Fig 1) These cassettes were both cloned into binary vectors under the control of the 35S promoter and terminator, and into vectors for in vitro transcription and translation ... disrupting protein–protein interactions within PSI, or by altering the conformation of the loop His-tags were employed in a topological study of the major light harvesting chlorophyll a ⁄ b binding...

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In Vitro Screening of Plant Resources for Extra-Nutritional Attributes in Ruminants: Nuclear and Related Methodologies pptx

Ngày tải lên : 15/03/2014, 04:20

... the initial screening is conducted in vitro because of the large number of candidate plant species and the prohibitive cost of screening them in vivo The number of species for in vivo testing ... emergence and wide-spread incidence of chemical residues in human food and antimicrobial and anthelmintic resistance causing a surge of interest in the use of “natural” alternatives to chemicals in ... to the methods involved in collecting, preparing and screening plants for bioactive properties for use in manipulating key ruminal fermentation pathways and against gastrointestinal pathogens...

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Báo cáo khoa học: Recent contributions of in vitro models to our understanding of hepatitis C virus life cycle pdf

Ngày tải lên : 16/03/2014, 05:20

... mutations are at the N-terminus of the NS3 helicase, in two distinct positions of NS4B, in the centre of NS5A and in the C-terminal region of NS5B [3–6] The significance of these mutations has been ... vitro HCV infection models This model has essentially been used in two major fields of research: binding process and vaccination development Specific binding of HCV-like particles was obtained for ... the poor binding inhibition achieved in the presence of mAbs directed against CD81 In contrast, heparin sulfate seemed to mediate this interaction [53] Taking advantage of this binding model, a...

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Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt

Ngày tải lên : 16/03/2014, 11:20

... ratio of apparent dissociation constants for binding GST-IjBa in the presence and absence of ATP, and the value of a indicates whether the binding of one substrate (ATP) affects the afnity of the ... In vitro analysis of NF-jB signaling pathway 46 Peet GW & Li J (1999) IkappaB kinases alpha and beta show a random sequential kinetic mechanism and are inhibited by staurosporine and quercetin ... = Intensity of nucleus Intensity of cytoplasm Intensity of nucleus Scheme This is a simplication of a cell as seen by analysis software, where the software measures the intensity of NF-jB in...

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Báo cáo khoa học: In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional doc

Ngày tải lên : 17/03/2014, 10:20

... available and its binding affinity is not altered by the presence of DNA in the DNA binding site Interestingly, when nM RNA and 10 nM DNA were added simultaneously to a limiting amount of protein in ... stringent concentration of KCl, there was a correlation between inhibition of endonuclease activity and inhibition of splicing activity indicating that RNA binding can be monitored by measuring ... filter binding assay in TK8 buffer with nM I-AniI and varying concentrations of end-labeled pCOBLE or uniformly labeled AnCOB pre-RNA RNA binding reactions contained mM MgCl2 and DNA binding reactions...

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Báo cáo khoa học: Chick embryo anchored alkaline phosphatase and mineralization process in vitro Influence of Ca 2+ and nature of substrates doc

Ngày tải lên : 23/03/2014, 17:21

... quality and the type of mineral formation obtained in the mineralization medium during the GPIbALP-induced hydrolysis of different phosphate substrates Ó FEBS 2003 Bone GPI-bALP, roles in mineralization ... interpretation of IR spectra of mineral deposits Distinct mineralization steps were observed during the GPI-bALPinduced hydrolysis of b-GP At incubation times in the range 1.5–3 h, two bands located ... J.E & Heersche, J.N.R (1991) Initiation and progression in mineralization of bone nodules formed in vitro: the role of alkaline phosphatase and organic phosphate Bone Mineral 14, 27–40 32 Coc, M.R.,...

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in vitro release of ketoprofen from proprietary and extemporaneously manufactured gels

Ngày tải lên : 13/04/2014, 09:25

... on the transdermal delivery of various molecules, both in vitro and in vivo These methods include in vitro and in vivo delivery of insulin, mannitol, glucose and heparin (17, 80) Phonophoresis ... events include extrusion of lamellar bodies, loss of nucleus and increasing amount of keratin in the stratum corneum (12) The origins of the cells of the epidermis lie in the basal lamina between ... Effect of molarity and pH on the diffusion profile of ketoprofen .85 Effect of temperature on the diffusion profile of ketoprofen 86 Effect of mass on the diffusion profile of ketoprofen ...

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báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx

Ngày tải lên : 18/06/2014, 15:20

... cells producing TGF-β resulted in the induction of Treg in vivo and in vitro in a murine model [52] Moreover, the blockade of TGF-β reduces Treg induction by the fusion cell vaccine and enhances ... (obtained with following informed consent and approved by our institutional review board obtained) was isolated and maintained in TIL Media I medium with 10% heat-inactivated FCS, mM L-glutamine, ... each vaccination [27,33] Autologous FCs were irradiated, suspended in 0.3 ml normal saline, and underwent up to nine times vaccinations via SC injection in the left inguinal area at 2-week intervals...

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Báo cáo sinh học: " In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strain" pot

Ngày tải lên : 18/06/2014, 18:20

... cells (cell lines and PBMC) and MYXV wild type (T1) strain or vaccinal (SG33) strain In bovine cell lines, serial viral passages analysis and infection with both T1 and SG33 expressing LacZ gene ... comparing two different MYXV strains (a wild-type strain (T1) and a cell-cultured attenuated vaccinal strain (SG33) [14]) we verified the stability of the viral tropism in vitro Findings Three bovine ... http://www.virologyj.com/content/4/1/94 information concerning interactions between MYXV and bovine cells is available yet In this study, we characterized the infection of bovine cell lines and bovine peripheral blood...

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Báo cáo sinh học: "In vitro migration of cytotoxic T lymphocyte derived from a colon carcinoma patient is dependent on CCL2 and CCR2" pot

Ngày tải lên : 18/06/2014, 19:20

... fixed in buffered formalin and embedded in paraffin a, b: Staining with H&E c, d: Specific brown staining of apoptotic cells by anti-caspase Ab e, f: In situ end-labeling (ISEL); black staining of ... cells resulting in tumor growth inhibition (reviewed in [25] and [1]) In mouse systems, ex vivo transduction of chemokines into tumor cells has provided potent tumor vaccines inducing tumor rejection, ... grown in vitro under three-dimensional conditions using a mixture of collagen and fibroblasts [32,44] Interaction of a2 and b1 integrins on CRC-specific T cells with collagen and the presence of...

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