in vitro evaluation of surfactants

... Abstract Skin aging is the result of internal and external factors So-called photoaging has been identified as the major factor in skin aging Effects of photoaging include inhibition of fibroblast ... In Vitro Cell.Dev.Biol.—Animal DOI 10.1007/s11626-013-9706-1 In vitro evaluation of the effects of human umbilical cord extracts on human fibroblasts, keratinocytes, and melanocytes ... umbilical cord extract-derived formulae on three kinds of skin cells including fibroblasts, keratinocytes, and melanocytes We prepared 20 formulae from intracellular umbilical cord extracts, extracellular

Ngày tải lên: 16/12/2017, 18:04

... permitting them to interact with biomolecules in a distinct way [2] This property has increased the possibility of surface engineering according to need in cancer therapy, cell imaging, bio-sensing ... less than that of unmodified ZnO NPs which could be due to coating of non-crystalline TritonX Corresponding miller indices obtained from Powder X software indicate crystalline planes of polygonal ... gunjanbisht31@gmail.com Department of? ?Chemical Science and Engineering, School of? ?Engineering, Kathmandu University, Dhulikhel, Nepal Full list of author information is available at the end of the article © 2016

Ngày tải lên: 29/05/2020, 14:12

... with 2% digitonin in PBS and the protein concentration was determined using the BCA assay kit For each sample, an aliquot of 50 μl of protein lysate containing μg/μl of protein in PBS was added ... apoptosis by activation of the intrinsic death pathway [15] As proof of concept, we found that OPH depletes GSH in the presence of S-NPAA in vitro as well as in cell lines overexpressing OPH (e.g LNCaP ... calculated by subtracting the absorbance of the well containing the control tube aliquot from the absorbance of the well containing the sample tube aliquot The amount of protein carbonyls in the sample

Ngày tải lên: 14/10/2020, 15:41

... Computational study and in vitro evaluation of the anti-proliferative activity of novel naproxen derivatives Abstract In the present work, five naproxen derivatives, i.e., 3-amino-(4E)-5-imino-1-[2-(6methoxy-2-naphthyl)propanoyl]-4-(benzylidene)-4,5-dihydro-1H-pyrazole ... activity of a great number of pyrazoles and naproxen moieties and in continuation of our research to syntheses of bioactive naproxen derivatives [8], we report herein the syntheses of Nnaproxenylpyrazole ... be gained in one-electron transfer mechanism The values of the ionization potential have been tabulated in Table S1 The trend in IP has been observed as c < a < b < d < e illuminating that in compound

Ngày tải lên: 19/11/2022, 11:42

... floating, swelling ability, and in vitro drug release was investigated Moreover, the in vivo cytoprotective effect of the selected formulation on gastric ulcers induced by indomethacin in rabbits ... Examination of Gastric Erosions Specimens of stomach of rabbits were fixed in 10% neutral-buffered formalin for 24 h, dehydrated in ascending grades of alcohol, cleared in xylene, and embedded in ... assessment of swelling, floating, bioadhesion, and drug release in 0.1 N HCl The optimized GR formulation was examined for its protective effect on the gastric ulcer induced by indomethacin in albino

Ngày tải lên: 11/05/2020, 12:02

... slow and sustained-release oral formulation of capsaicin is urgently required to maintain sustainable levels of capsaicin in the blood and lessen stimulation of the gastrointestinal tract Studies ... chosen for oral administration in our study The in vivo pharmacokinetic behavior of free capsaicin, SDC, and SMPC were investigated following oral administration of 60 mg/kg of capsaicin to 18 healthy ... preparation of its sustained-release matrix pellets KEY WORDS: capsaicin; in vitro release; oral bioavailability; pharmacokinetic studies; sustained-release pellets INTRODUCTION Capsaicin, belonging

Ngày tải lên: 11/05/2020, 12:07

... evaluated in vitro in several established human colorectal cell lines Since the in vitro setup does not allow us taking into account the influence of the tumor-micro environment, the most promising ... Results: In the tested cell lines, the FGFR inhibitor (JNJ-42756493) was effective in vitro and in vivo in CRC tumors with highest expression of FGFR2 (NCI-H716) In vitro, cell proliferation in this ... dose rate of 3Gy/min For the NCI-H716 xenograft model, a single dose of Gy was used Tumors of the CaCo2 xenograft model were irradiated with a single dose of 10Gy The in vitro and in vivo radiation

Ngày tải lên: 22/09/2020, 23:07

... to have little effect on in vitro binding, internalization, or in vivo pharmacokinetics The in vitro binding of DOTA-PEG-BN(7-14) to GRPR was lower than the binding of DOTAAoc-BN(7-14) (Fig 3) ... corresponding to the retention time of DOTA-PEGBN(7-14) was collected, concentrated and re-suspended for in vitro and in vivo evaluation In vitro Evaluation A representative competitive binding assay ... peptide (64Cu-DOTA-Aoc-BN(7-14)) containing an eight carbon spacer in place of PEG in vitro using GRPRexpressing PC-3 human prostate cancer cells and in vivo in normal athymic nude mice The 64CuDOTA-Aoc-BN(7-14)

Ngày tải lên: 21/10/2022, 15:17

... studied in all possible combinations are considered to be the most efficient in estimating the influence of individual variables and their interactions using minimum experiments The application of ... was determined by using the following equation Weight of sample- weight of detached partiicles ×100 Percentagemucoadhesion = Weight of sample In vitro swelling studies The swellability of microspheres ... technique utilizing a 23 factorial design The effect of formula variables, such as drug: polymer ratio, concentration of cross‑linking agent and cross‑linking time on the particle size and in? ?vitro mucoadhesion

Ngày tải lên: 02/11/2022, 10:41

... Jianlin Gong6 and Hisao Tajiri1 Address: 1Division of Gastroenterology and Hepatology, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan, 2Institute of Clinical ... Medicine and Research, The Jikei University School of Medicine, Tokyo, Japan, 3Department of Oncology, Institute of DNA Medicine, The Jikei University School of Medicine, Tokyo, Japan, 4Clinical ... generation of Treg through the inhibition of DCs maturation; however, fusion cell vaccination results in recovery of the DCs function and induction of antigen-specific CTL responses in vitro The

Ngày tải lên: 18/06/2014, 15:20

... adding T cells), fixed in buffered formalin and embedded in paraffin. a, b: Staining with H&E. c, d: Specific brown staining of apoptotic cells by anti-caspase 3 Ab. e, f: In situ end-labeling ... through binding of CCL2 to its corre- sponding chemokine receptor CCR2 on the T cells. This was demonstrated by blocking of T-cell migration in presence of addition of excess chemokine CCL2 in the ... implicated in tumor rejection induced by vaccination of mice with CCL19-transduced tumor cells [26], and CD8 + CTL were instrumental in tumor growth rejection in mice following intratumoral delivery of

Ngày tải lên: 18/06/2014, 19:20

... prove the feasibility of detecting several forms of femoral implant loosening, in vitro and in vivo using techniques based on harmonic distortion [16-19]. In vitro, the analysis of frequency response ... The influence of contact conditions on resonance frequencies of a hip stem- femur system: a finite element analysis. In Proceedings of the International seminar Prediction and evaluation of total ... tem for intra operative manufacturing and stability testing of hip prostheses. In Proceedings of TEHNOMUS XIII, the 13th Inter- national conference on New technologies and products in machine manu-

Ngày tải lên: 20/06/2014, 01:20

... loading of protein was validated by re-blotting the same membranes with anti-β-actin. The data is representative of three independent experiments. (B) Increase in the expression of MnSOD in ... of Se on viral infection. Se deficiency increases apoptosis in WNV-infected Vero cells Vero cells were inoculated with WNV NY99 strain at mul- tiplicity of infection (MOI) 1, at day 7 post-induction ... virus infection, it is important to identify cell lines in which Se deficiency can be efficiently induced in vitro without compromising cell viability. Based on the Se-deficient in vitro and in

Ngày tải lên: 20/06/2014, 01:20

... adding T cells), fixed in buffered formalin and embedded in paraffin. a, b: Staining with H&E. c, d: Specific brown staining of apoptotic cells by anti-caspase 3 Ab. e, f: In situ end-labeling ... through binding of CCL2 to its corre- sponding chemokine receptor CCR2 on the T cells. This was demonstrated by blocking of T-cell migration in presence of addition of excess chemokine CCL2 in the ... implicated in tumor rejection induced by vaccination of mice with CCL19-transduced tumor cells [26], and CD8 + CTL were instrumental in tumor growth rejection in mice following intratumoral delivery of

Ngày tải lên: 20/06/2014, 03:20

... 5 min of incubation with 0.4% Trypan blue, the percentages of stained cells (indicative of nonviable cells) versus stain-excluding cells were counted in a hemocytometer. Then the percentage of ... to remove traces of FCS. After remov- ing HBSS, the cells were trypsinized with 3 ml of trypsin- EDTA (BioWhittaker™ Cambrex Bio Science). After exam- ining the cells using an inverted microscope ... disease in joints. The incidence of OA in humans is 12.1% of the popula- tion between 25-74 years of age [1]. Reports on OA epide- miology consistently show an almost exponential increase of prevalence

Ngày tải lên: 20/06/2014, 04:20

... display a range of activities inside the cell ranging from redox catalysis to the tar- geted binding of biomolecules [14-17]. Recent findings [18-26] indicate that many types of nanoparticles ... reproduction in a ny medium, provided the original work is properly cited. capable of inducing oxidative stress, which i s of great concern in terms of the nanotoxicology of particles being pursued ... contained in the original loading solution deter- mined Cu loading, resulting in values ranging between 12 and 16 wt.% based on these reactions conditions. XPS was used to further confirm Cu loading

Ngày tải lên: 21/06/2014, 02:20

... albumin. IN VITRO MATURATION OF OOCYTES IN INFERTILITY TREATMENTS The research into maturation of immature oocytes initiated by Pincus and Enzmann (8) and continued by Edwards et al. (23) was not incorporated ... perinatal outcome in pregnancies following IVM, IVF, and ICSI delivered in a single center In: Proceedings of the 61st Annual Meeting of the American Society for Reproductive Medicine, ... Intravenous fentanyl is administered at intervals of 15–20 min up to a total dose of 150–200 mg Local in ltration of bupivacaine 0.5% in the vagina reduces the discomfort of multiple needle

Ngày tải lên: 05/08/2014, 16:20

... high inhibition of CCV replication in CCO up to 48 hr post-infection, which decreased slightly by 84 hr post-infection All remaining extracts tested against CCV in CCO cells were determined to ... attachment/entry inhibition assays This is particularly pertinent in confined marine habitats that can be seeded with the active elements of these extracts in hopes of preventing the spread of viral ... cell line easier than another, thus possibly producing some replication inhibition in one cell line and not the other Further testing is needed to identify any of these contributing or limiting...

Ngày tải lên: 12/08/2014, 04:20

... surfaces, allowing for a controlled comparison of sperm binding Thus, the variation in binding capacity between individual ZP is eliminated The binding capacity of two semen samples to matching hemizonae ... is staining with fluoresceinconjugated lectins, such as Peanut Agglutinin (PNA), Pisum Sativum Agglutinin (PSA) or Concanavalin A (ConA) coupled with fluoresceinisothiocynate (FITC) (Magistrini et ... videomicrographic evaluation of stallion semen before and after freezing in a diluent containing egg yolk) Thesis, Hannover 1991 Sammandrag Bedömning av frys-tinad hingst sperma med hjälp av in vitro metoder...

Ngày tải lên: 12/08/2014, 15:20

... degree of Master of Engineering in the Department of Mechanical Engineering at the National University of Singapore under the supervision of Professor Teoh Swee Hin and Dr Alvin Yeo No part of this ... 1.3.2 Part 2: Optimization of native and customized scaffolds in vitro and their effects in initial bone healing 1.3.3 Part 3: Evaluation of PCL-TCP scaffolds in a clinically relevant defect model ... CHAPTER 4: OPTIMIZATION OF NATIVE AND CUSTOMIZED SCAFFOLDS IN VITRO AND THEIR EFFECTS IN INITIAL BONE HEALING 4.1 INTRODUCTION 44 4.1.1 In vitro degradation study 44 4.1.2 In vivo degradation study...

Ngày tải lên: 09/10/2015, 11:18