in vitro evaluation of surfactants
báo cáo khoa học: " In vitro evaluation of various bioabsorbable and nonresorbable barrier membranes for guided tissue regeneration" pptx
... were maintained in DMEM (Invitrogen, Carlsbad, CA, USA) containing 1% penicillin/streptomycin (Invitrogen, Carlsbad, CA, USA), 1% fungizone (Sigma, St Louis, MO, USA) and 10% fetal bovine serum ... and the inflammatory reaction of the material Further confirmation of this hypothesis is required Competing interests One must be cautious when interpreting results obtained by using in vitro experimental ... to be common problems of nonresorbable membranes resulting in lower probing attachment level gains in intrabony defects [8] In order to overcome these issues, a variety of bioabsorbable materials,...
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Báo cáo y học: " In vitro evaluation of marine-microorganism extracts for anti-viral activity" pot
... high inhibition of CCV replication in CCO up to 48 hr post-infection, which decreased slightly by 84 hr post-infection All remaining extracts tested against CCV in CCO cells were determined to ... attachment/entry inhibition assays This is particularly pertinent in confined marine habitats that can be seeded with the active elements of these extracts in hopes of preventing the spread of viral ... cell line easier than another, thus possibly producing some replication inhibition in one cell line and not the other Further testing is needed to identify any of these contributing or limiting...
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Báo cáo khoa học: "In Vitro Evaluation of Frozen-Thawed Stallion Semen: A Review" doc
... surfaces, allowing for a controlled comparison of sperm binding Thus, the variation in binding capacity between individual ZP is eliminated The binding capacity of two semen samples to matching hemizonae ... is staining with fluoresceinconjugated lectins, such as Peanut Agglutinin (PNA), Pisum Sativum Agglutinin (PSA) or Concanavalin A (ConA) coupled with fluoresceinisothiocynate (FITC) (Magistrini et ... videomicrographic evaluation of stallion semen before and after freezing in a diluent containing egg yolk) Thesis, Hannover 1991 Sammandrag Bedömning av frys-tinad hingst sperma med hjälp av in vitro metoder...
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Báo cáo khoa học: "In vivo and in vitro evaluation of an Acetobacter xylinum synthesized microbial cellulose membrane intended for guided tissue repair" ppt
... becoming a subject of increasing interest because of their potential in tissue engineering applications [20] They can be obtained from adult bone marrow and are capable to differentiate into ... ratios, strain of Acetobacter and extended times of fermentation interfere in the final product obtained [2,19] In addition, the choice of a particular cellulose structure will depend on the clinical ... Committee at the School of Veterinary Medicine and Animal Science, São Paulo State University Before surgery, anesthesia was induced by intramuscular injection of a combination of xylazine 2%, 10 mg/kg...
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Báo cáo sinh học: " In vitro evaluation of a double-stranded selfcomplementary adeno-associated virus type2 vector in bone marrow stromal cells for bone healing" pptx
... non-enveloped single-stranded DNA virus [19] This unique viral vector has the distinct advantage of being capable of infecting a wide range of host cell types including dividing and non-dividing cells ... a distinct affinity for certain cell types that is because of differences in cell binding and/or intracellular trafficking [21] AAV2 is the most widely used serotype in human clinical trials ... broad range of tissue tropism in several species [21] A number of clinical trials have been approved by the FDA to assess AAV2 in treatment of a variety of human diseases including inflammatory...
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Báo cáo y học: "In vitro and in vivo evaluation of a new active heat moisture exchange" potx
... the offset was maintained, with no significant variations We used this offset (in the order of 0.1–0.2°C) to correct the measurements obtained during the study In each condition, the average of ... is increased [16,30], causing an increase in carbon dioxide levels, especially during low tidal volume ventilation [31] HMEs also cause an increase the inspiratory work of breathing, with an increase ... 30 mgH2O/l) when using HMEs in patients with high minute ventilation (between 10.5 and 16.5 l/min) [19,23,24] In the present study we found that increasing or decreasing the minute ventilation...
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In vitro and in vivo evaluation of customized polycaprolactone tricalcium phosphate scaffolds for bone tissue engineering
... degree of Master of Engineering in the Department of Mechanical Engineering at the National University of Singapore under the supervision of Professor Teoh Swee Hin and Dr Alvin Yeo No part of this ... 1.3.2 Part 2: Optimization of native and customized scaffolds in vitro and their effects in initial bone healing 1.3.3 Part 3: Evaluation of PCL-TCP scaffolds in a clinically relevant defect model ... CHAPTER 4: OPTIMIZATION OF NATIVE AND CUSTOMIZED SCAFFOLDS IN VITRO AND THEIR EFFECTS IN INITIAL BONE HEALING 4.1 INTRODUCTION 44 4.1.1 In vitro degradation study 44 4.1.2 In vivo degradation study...
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In vitro and in vivo evaluation of transferrin conjugated lipid shell and polymer core nanoparticles for targeted delivery of docetaxel
... THE DEGREE OF MASTER OF ENGINEERING DEPARTMENT OF CHEMICAL AND BIOMOLECULAR ENGINEERING NATIONAL UNIVERSITY OF SINGAPORE 2011 Acknowledgements First of all, I would like to express my profound gratitude ... discussed in Chapter In Chapter 5, in vitro cellular study of transferrin conjugated LPNPs is performed using MCF7 human breast adenocarcinoma cells In Chapter 6, in vivo pharmacokinetics, complete ... protein binding after IV administration (Extra et al., 1993) Docetaxel is mainly bound to alpha acid glycoprotein, lipoproteins, and albumin Among them, alpha acid glycoprotein is the main determinant...
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Stability and in vivo evaluation of pullulan acetate as a drug nanocarrier
... were fixed in 10% phosphate buffered formalin, embedded in paraffin, sectioned, and stained with hematoxylin and eosin (H&E) In vivo pharmacokinetics and bioavailability In vivo pharmacokinetic study ... i.v at a dose of 200 mg/kg In vivo pharmacokinetics The plasma levels of EPI were determined following a single i.v injection of EPI or PA/EPI (10 mg/kg EPI equiv.) in female Wistar rats The plasma ... drug concentration of EPI and PA/EPI after i.v injection in rats at a single equivalent dose of 10 mg/kg Table 5. Pharmacokinetic parameters of EPI and PA/EPI i.v injected in rats at the equivalent...
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Analysis, sequencing and in vitro expression of PCR products
... also protein fragments for mapping of interaction domains The use of in vitro expression approaches to analyze protein products is likely to gain momentum for the analysis of known proteins as well ... functionally analyzed using in vitro assays Furthermore, rapid in vitro protein–protein interaction studies could be performed using this technique where not only full-length version of proteins could be ... original primers Of course in this case the use of an enzyme with 3′-exonuclease proofreading activity should be avoided to protect the differentiating 3′-end An example of the design of original...
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Tài liệu Aesthetic preferences and the attribution of meaning: Environmental categorization processes in the evaluation of urban scenes ppt
... provide important information in terms of optimizing processes of environmental sampling To overcome some of the above-mentioned limitations, an evaluation procedure was designed in a previous study ... subjects interviewed Exploring the generality of such a categorization in other geographical scenes, incorporating the analysis of places considered ‘‘unattractive,’’ is one of the aims of the ... familiarity or recognition of visualized scenes may play in processes of aesthetic evaluation The development of evaluation procedures aimed at identifying categories of known and/or familiar places...
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PET in the Evaluation of Alzheimer’s Disease and Related Disorders docx
... Future of Neuroimaging in Clinical Evaluations Innovations in neuroimaging techniques and tracer development hold promise for new clinical applications Brain imaging will play an important role in ... friend of many years who has always sportingly accepted my invitations to participate in any number of forums of symposia and writing projects and has once again offered his insights into the ... Small Clinical Interpretation of Brain PET Scans: Performing Visual Assessments, Providing Quantifying Data, and Generating Integrated Reports Daniel H.S Silverman 33 FDG PET in the Evaluation...
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Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf
... reaction by using 32P-labeling primer or template in the presence or absence of DNA-binding agents (DDIs and doxorubicin) The DNA bulge binding of both compounds was detected by CD and UV melting experiments ... is formed To gain insight into the binding of drugs to bulge DNA, several bulgecontaining oligonucleotides were selected as binding hosts for DDI-1A and DDI-1B CD spectroscopy of DDI-1A (Fig ... two-base bulge in DNA via stacking of its helically oriented aromatic ring systems on the bulge-flanking base pairs that define the long sides of the triangular prism binding pocket, with its amino sugar...
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Báo cáo khoa học: The in vitro effects of CRE-decoy oligonucleotides in combination with conventional chemotherapy in colorectal cancer cell lines potx
... alone did not increase the extent of staining However, in cells that had been cocultured with CDO and a cytotoxic drug, staining was significantly increased, indicating the presence of senescence ... provide a possible model of drug interaction in GEO cells, which involves the complex interaction of proteins involved in cell cycle regulation To recapitulate, combining CDO with cytotoxic chemotherapy ... analysis of BrdU incorporation The degree of incorporation of the thymidine analogue 5-bromo-2¢-deoxyuridine (BrdU; Sigma) in HCT116 and GEO cells was measured by flow cytometry Following culture in...
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In Vitro Screening of Plant Resources for Extra-Nutritional Attributes in Ruminants: Nuclear and Related Methodologies pptx
... challenges of extrapolating in vitro findings to in vivo evaluation of plant resources The chapters in this book are written by experts interested in exploring and making better use of plant biodiversity ... the initial screening is conducted in vitro because of the large number of candidate plant species and the prohibitive cost of screening them in vivo The number of species for in vivo testing ... remaining larvae into individual labelled petri dishes and stain with a few drops of helminthological iodine 10 Add 50 μL of helminthological iodine to each well on the culture plate 11 Using...
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Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
... (Fig 3E) Interestingly, the inclusion of IKK2 inhibition by SC-514 with our experimentally determined rate constants in the original model resulted in a delay in simulated peak and damping of subsequent ... simulated in silico Combining this method of analysis (in vitro, cell-based and in silico analysis) will facilitate systematic understanding of the underlying properties of this signaling pathway ... = Intensity of nucleus Intensity of cytoplasm Intensity of nucleus Scheme This is a simplication of a cell as seen by analysis software, where the software measures the intensity of NF-jB in...
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Báo cáo khoa học: In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional doc
... stringent concentration of KCl, there was a correlation between inhibition of endonuclease activity and inhibition of splicing activity indicating that RNA binding can be monitored by measuring ... and its binding affinity is not altered by the presence of DNA in the DNA binding site Interestingly, when nM RNA and 10 nM DNA were added simultaneously to a limiting amount of protein in TK8 buffer ... background In vitro competition assays RNA splicing and DNA cleavage competition experiments both involved a prebinding step in which either DNA or RNA substrates were incubated with I-AniI in a binding...
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Báo cáo khoa học: Chick embryo anchored alkaline phosphatase and mineralization process in vitro Influence of Ca 2+ and nature of substrates doc
... in the GPI-bALP mediated mineralization: effects of Ca2+ concentration To assess the role of Ca2+ in initiating the mineralization process, we modelled the biological process by using 0.8 U of ... presence of anchored GPI-bALP (Fig 1) The presence of HA in the mineral samples was confirmed by In uence of other phosphate substrates in the mineralization process To examine the effects of substrates ... of HA No mineral material was formed in media lacking GPI-bALP Mineral calcium phosphate identification by infrared spectra To identify the quality and the type of mineral formation obtained in...
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Báo cáo khoa học: In vitro characterization of a plastid terminal oxidase (PTOX) pot
... types (Fig 1A,B) in the presence of 0.2 mM of ubiquinone and, to some extent, of dPQ, duroquinone or vitamin K1 (phylloquinone) In contrast, addition of benzoquinone did not lead to such changes ... by Fisher and Rich [20] for quinone binding sites Although speculative at this stage, this consensus sequence was found in various quinone-binding proteins including AOX [20,21] It should be mentioned ... quinone binding site would be expected to be close to iron binding amino acids, which is the case here ([6], Fig 4) This potential quinone-binding site appears to be conserved in PTOX sequences (including...
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in vitro release of ketoprofen from proprietary and extemporaneously manufactured gels
... Effect of molarity and pH on the diffusion profile of ketoprofen .85 Effect of temperature on the diffusion profile of ketoprofen 86 Effect of mass on the diffusion profile of ketoprofen ... variability in percutaneous absorption, the precision of dosing, the reservoir capacity of the skin, heterogeneity and inducibility of the skin in turnover and metabolism, inadequate definition of bioequivalence ... include extrusion of lamellar bodies, loss of nucleus and increasing amount of keratin in the stratum corneum (12) The origins of the cells of the epidermis lie in the basal lamina between the...
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