The Crystal Structure of the [NiFe] Hydrogenase

... experiments to elucidate the structure of the ready and unready states of the [NiFe] hydroge- The Structure of the A vinosum [NiFe] Hydrogenase 65 66 67 68 69 70 71 72 73 74 75 nase of Desulfovibrio ... to an oxygen atom in the case of the D vulgaris MF hydrogenase in the Ni-A state and in the case of the D gigas hydrogenase in the Ni-SU sta...

Ngày tải lên: 21/12/2016, 10:50

... A2 for main-chain atoms, 35.7 A2 for side-chain ˚ overall (3 244 atoms) For the pepatoms and 33.8 A tide, B factors are slightly higher than those for ˚ ˚ importin -a: 36.9 A2 for main-chain atoms, ... atoms, 40 .2 A2 for ˚ overall (62 total atoms) side-chain atoms and 38.7 A Electron density analysis Both the main-chain and side-chain atoms of the modelled CLIC4 peptide...

Ngày tải lên: 14/02/2014, 19:20

... illustrates the superimposition of ApeSOD with the cambialistic SOD from P shermanii The overall structures of these enzymes were similar; the rmsd of the 747 Ca atoms was ˚ 0.695 A ApeSOD eluted from the ... superoxide dismutases Biochim Biophys Acta 1804, 24 5–2 62 Crystal structure of SOD from A pernix K1 Sugio S, Hiraoka BY & Yamakura F (2000) Crys...

Ngày tải lên: 14/02/2014, 22:20

... substrate recognition For the superposition of the substrate- free PhyK with substrate- free ˚ AppA the Ca atoms are 2.41 A apart, whereas for the substrate- free PhyK and the substrate- loaded AppA ... B Fig Crystal structure of Klebsiella sp ASR1 phytase (A) Cartoon representation showing the two domains of PhyK, the a domain (orange) and the a ⁄...

Ngày tải lên: 16/02/2014, 09:20

... 14-48 form the small insertion domain that comprises a short a- helix and a three-stranded anti-parallel b-sheet; the remaining protein residues form the (a ⁄ b)8 barrel domain and a C-terminal extension ... would act as a regulatory link between glycolysis and NAD synthesis The structure of hACMSD in complex with DHAP may used for the design o...

Ngày tải lên: 18/02/2014, 06:20

... structure of the catalytic domain of DESC1 Scheme Domain organization of human DESC1 membrane region The extracellular part of DESC1 consists of a 120-amino acid SEA domain followed by the C-terminal ... growth factors and proteinase inhibitors Biol Chem 380, 473–483 Kataoka H, Uchino H, Asada Y, Hatakeyama K, Nabeshima K, Sumiyoshi A & Koono M (1997) Analy- C...

Ngày tải lên: 19/02/2014, 00:20

... References Ivanova N, Sorokin A, Anderson I, Galleron N, Candelon B, Kapatral V, Bhattacharyya A, Reznik G, Mikhailova N, Lapidus A et al (2003) Genome sequence of Bacillus cereus and comparative analysis ... of GAB catalysis have been identified to date [12] which are based either on a single, bifunctional GAB catalyst or on a GAB catalysts pair The available biochemical dat...

Ngày tải lên: 19/02/2014, 00:20

... the carboxyl oxygen atoms of the side chains of Asp11, Asp14 and Asp21, the amide oxygen atom of Gln15 and the carbonyl oxygen atoms of Asp11 and Asn23 (Fig 3) in an arrangement similar to what ... within a few days to thin hexa62 Table Data collection and refinement statistics for SPRK ˚ Resolution (A) Space group Cell parameters ˚ a- axis (A) ˚ b-axis (A) ˚...

Ngày tải lên: 19/02/2014, 07:20

... Evidence for the presence of DNA-binding proteins involved in regulation of the gene expression of indole-3pyruvic acid decarboxylase, a key enzyme in indole-3-acetic acid biosynthesis in Azospirillum ... the aminopyrimidine ring, are conserved in all ThDP-dependent enzymes One of these, the hydrogen bond between the N1¢ atom of the pyrimidine ring...

Ngày tải lên: 20/02/2014, 11:20

... and serves as an inhibitor for diol and glycerol dehydratases [57] Overall structure of glycerol dehydratase in complex with cobalamin and propane-1,2-diol The crystal structure of glycerol dehydratase ... dehydratase- bound cobalamins (2.9–5.1°) [9,10] Figure 4C indicates the comparison of the position of the a-acetamide side chain of pyrrol...

Ngày tải lên: 21/02/2014, 03:20

... report the crystal structure of the intact glutaminase under four different conditions: in the absence of the additives (referred to as N); in the presence of Tris (referred to as T); in the presence ... determined the F structure containing the truncated region of the C-terminal domain; however, we failed to determine the structure of th...

Ngày tải lên: 06/03/2014, 09:22

... aaagtgaagtcc-3¢ and 5¢-attattggatccctattgctggtccgattctgccag-3¢ 3-TPR NlpI primers (residues 62–197) were 5¢-aataatccatgg gggcacagcttttatatgagcgcggag-3¢ and 5¢-aataatggatcctcactgttc cttatccgatttttcgaagtgc-3¢ ... (Finland) Data for this study were measured at beamline X12C of the National Synchrotron Light Source We are grateful for the assistance of Dr Anand Saxena in the use of...

Ngày tải lên: 07/03/2014, 16:20

... Crystallization and preliminary X-ray analysis of a Trx domain of human thioredoxin-like protein Acta Crystallogr 57, 1712–1714 32 Otwinowski, Z & Minor, W (1997) Processing of X-ray diffraction data ... identity, dissimilar crystal forms and dissimilar intermolecular contacts near the active site in the crystal, the conformation of the active site (-Cys-Gly-ProCys...

Ngày tải lên: 08/03/2014, 22:20

... on the presence or absence of the Isp1 protein The expression of the Hup enzyme depends on the thiosulfate content of the medium With a view to examining the function of the HupC protein in T roseopersicina, ... synthesis [9] Next to the hupSL Electron-transfer subunits of NiFe hydrogenases genes encoding for the small and large hydrogenase subunits,...

Ngày tải lên: 16/03/2014, 03:20

... N-terminus) (B) Zoom -in view of the interactions of papain with loop L6 of chagasin and loop L2 of cystatin B (C) Zoom -in view of the interactions of papain with loop L4 of chagasin and the N-terminal ... 3) The difference of 9° between these complexes may be correlated with the variation of the rate of binding and affinity for chagasi...

Ngày tải lên: 16/03/2014, 04:20