... produce pET19b(H6bG) A pair of synthetic oligonucleotides, 5¢-TATGGGCAGCAGCCATCATCATCATCATCACA GCAGCGGCCTGGTGCCGCGCGGCAGCAC-3¢ and 5¢-TAGTGCTGCCGCGCGGCACCAGGCCGCTGCT GTGATGATGATGATGATGGCTGCTGCCCA-3¢ ... glycerol dehydratase were amplified by PCR using pairs of primers 5¢-CATATGCAACAGACAACCCAAATTCAGCCC-3¢ and 5¢-AGATCTTATCACTCCCTTACTAAGTCGATG-3¢ for the b subunit and 5¢-CATATGAGCGAGAAAACCA TGCGCGTGCAG-3¢ ... N (20 00) How a protein generates a catalytic radical from Ó FEBS 20 02 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 Structureof B 12- dependent glycerol dehydratase (Eur J Biochem 26 9)...
... program to check the stereochemical quality of protein structures J Appl Crystallogr 26 , 28 3 29 1 27 Schuck P (20 00) Size-distribution analysis of macromolecules by sedimentation velocity ultracentrifugation ... Analysis of a catalytic acidic pair in the active center of cellulase from Aspergillus aculeatus Biosci Biotechnol Biochem 63, 21 57 21 62 33 Lever M (19 72) A new reaction for colorimetric determination ... determined the crystalstructureof Bg7S, which is the first three-dimensional structure FEBS Journal 27 8 (20 11) 1944–1954 ª 20 11 The Authors Journal compilation ª 20 11 FEBS 1949 Structureof Bg7S,...
... Y457G mutant was constructed using the QuikChangeÒ procedure (Stratagene) with primers 5¢-TCTTCAGCGGC ATGGGCGACCTCGGCGGCAT-3¢ and 5¢-ATGCCGC CGAGGTCGCCCATGCCGCTGAAGA-3¢ The recombinant protein ... the accuracy ofcrystal structures Nature 355, 4 72 475 21 Emsley P & Cowtan K (20 04) Coot: model-building tools for molecular graphics Acta Crystallogr D 60, 21 26 21 32 22 Potterton L, McNicholas ... Y (20 02) Purification, characterization, cloning, and expression of a novel xyloglucan-speci c glycosidase, oligoxyloglucan reducing end-speci c cellobiohydrolase J Biol Chem 27 7, 4 827 6–4 828 1...
... 550b 3 1c > 61b 34. 3c 0.6 5c 0.5 2c 0 .2 1c 0.4 2c Nitrocefin Cephalexin Cefazolin Benzylpenicillin Ampicillin Carbenicillin Oxacillin Cloxacillin 116b 5 0c > 150b 2. 3d 5.1d 3.7d 0.118d 0.016d kcat (S)1) ... linear dependence of the FEBS Journal 27 5 (20 08) 1687–1697 ª 20 08 The Authors Journal compilation ª 20 08 FEBS C Michaux et al Psychrophilic class C b-lactamase intrinsic fluorescence of the native ... marcescens kcat ⁄ Km (lM)1Æs)1) C Michaux et al 0.8 0.6 0.4 0 .2 0 Urea concentration (M) Fig Intrinsic fluorescence of the Pse fluorescens TAE4 as a function of the urea concentration at 30 C FEBS...
... differences in the cyclophilin domain of AtCyP38 and other known cyclophilin structures (section 4.9 .2) The helical domain and the cyclophilin domain of the structure are connected β1 α1 22 GGILLVANPVIPDVSVLISGPPIKDPEALMRYAMPIDNKAIREVQKPME ... Unit-cell parameters Space group C2 22 1 Native Se-Met a (Å) 58 .2 58.1 b (Å) 95.9 96.0 c (Å) 167.5 167 .2 No of molecules in ASU 1 Data collection X-ray source and detector BNL (X -25 ) / ADSCQ-315 CCD ... Asp255 has clusters of charged residues with a net surplus of acidic residues (21 acidic, 16 basic) In other high molecular weight immunophilins, such as CyP40 and FKBP 52, similar clusters of charged...
... Coordinating atoms of calcium and distances Calcium No Interacting Atoms ASP 44 OD1 ASP 46 OD1 ASP 48 OD1 ILE 50 O ASP 52 OD2 WAT 27 Distance in Å 2. 47 2. 49 2. 50 2. 45 2. 48 2. 70 Calcium No Interacting Atoms ... Distance in Å 2. 59 2. 50 2. 64 2. 58 2. 59 2. 70 Ca 1, the calcium ion, which is coordinated by residues within 44 to 52, is present in most α-amylases, Figs 2. 7, 2. 8 Also, a novel calcium site, Ca2, ... data collection and crystallographic statistics are summarized in Table 2. 1 40 Figure 2. 5 lAmyA crystal picture Crystals of AmyA, with maximum dimensions of 0.1 x 0.1 x 0.6 mm Table 2. 1 Crystal...
... 0 .2 61 ± 2. 7 ± 0.1 FEBS Journal 27 8 (20 11) 22 83 22 95 ª 20 11 The Authors Journal compilation ª 20 11 FEBS J P Kallio et al Crystalstructureof a Thielavia arenaria laccase Fig (A) The crystalstructure ... endospore coat laccase from Bacillus subtilis J Biol Chem 27 9, 23 4 72 23 476 21 Li X, Wei Z, Zhang M, Peng X, Yu G, Teng M & Gong W (20 07) Crystal structures of E coli laccase CueO at different copper concentrations ... pocket are completely different, possibly accounting for the clear differences in reaction kinetics between basidiomycete laccases and asco-laccases Dimerization In the TaLcc1 crystal structure, ...
... expression of a chloride ion channel of cell nuclei J Biol Chem 27 2, 125 75– 125 82 28 Hu W & Jans DA (1999) Efficiency of importin alpha ⁄ beta-mediated nuclear localization sequence recognition and nuclear ... protein crystallography Acta Crystallogr D: Biol Crystallogr 50, 760–763 37 Evans P (20 06) Scaling and assessment of data quality Acta Crystallogr D: Biol Crystallogr 62, 72 82 38 McCoy AJ, Grosse-Kunstleve ... importin-a (Table 2) The numbers of contacts are comparable with those of the basic residues at P2 (Lys203: 31 contacts), P3 (Lys204: 20 contacts) and P5 (Arg206: 45 contacts) Normalized B factor analysis...
... (%) GlcNAc (GlcNAc )2 (GlcNAc)3 (GlcNAc)4 (GlcNAc)5 (GlcNAc)6 100 89. 82 10.51 5.51 12. 19 3.93 molecule in the active site and the binding of acetate, strongly suggest that BcZBP acts as a zinc-dependent ... Journal compilation ª 20 07 FEBS V E Fadouloglou et al Crystalstructureof BcZBP from B cereus Fig Model of the BcZBP–GlcNAc complex Stereoview of the energy minimized putative BcZBP–GlcNAc complex ... formation of two, FEBS Journal 27 4 (20 07) 3044–3054 ª 20 07 The Authors Journal compilation ª 20 07 FEBS 3045 Crystalstructureof BcZBP from B cereus V E Fadouloglou et al A B Fig Structureof the BcZBP...
... enzymes: molecular basis of cold adaptation Cell Mol Life Sci 53, 830–841 25 Feller G (20 03) Molecular adaptations to cold in psychrophilic enzymes Cell Mol Life Sci 60, 648–6 62 26 Kristjansson ... not yet clear to what extent this interaction would affect PRK specificity at the S2 site FEBS Journal 27 3 (20 06) 61–71 ª 20 05 The Authors Journal compilation ª 20 05 FEBS 65 Structureof proteinase ... the S1¢ and S2¢ binding site of SPRK The 2fofc electron density is contoured at 1.0 r FEBS Journal 27 3 (20 06) 61–71 ª 20 05 The Authors Journal compilation ª 20 05 FEBS 67 Structureof proteinase...
... intermolecular interactions, less FEBS Journal 27 2 (20 05) 8 32 845 ª 20 05 FEBS Structural aspects of cold adaptation compact packing of the hydrophobic core, an increased apolar surface area, decreased ... D188–K17 E28–K95 D 124 –K153 D188–R270 D201–R249 E253–R249 D257–R270 D257–K275 2. 97 3.00 3.91 2. 79 3 .20 2. 81 3.41 2. 98 2. 80 2. 78 ˚ A ˚ A ˚ A ˚ A ˚ A ˚ A ˚ A ˚ A ˚ A ˚ A a The criterion of conserved ion ... (20 05) 8 32 845 ª 20 05 FEBS ´ ´ J Arnorsdottir et al 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 Structural adaptations to cold and investgation of the active site J Biol Chem 27 8, 7531–7539...
... Bre´sil (COFECUB) (contract no 29 4 H99) which is fully acknowledged We acknowledge La socie´te´ de Secours des Amis des Sciences and the European Cooperation in the Field of Scienti c and Technical ... Biolabs) with the corresponding reaction buffer PCR was carried out using the following programme: first at 95 C; 30 cycles of at 95 C, at 50 C, at 72 C; a final incubation of 10 at 72 C The amplified ... Park, H.W (20 00) Structural analysis of glyceraldehyde-3-phosphate dehydrogenase from Escherichia coli: direct evidence of substrate binding and cofactorinduced conformational changes Biochemistry...
... (20 04) Developments in the CCP4 molecular-graphics project Acta Crystallogr D Biol Crystallogr 60, 22 88 22 94 44 Potterton E, McNicholas S, Krissinel E, Cowtan K & Noble M (20 02) The CCP4 molecular-graphics ... Arg 223 and Ser 227 interact with Tris Tris binding in the TG structure seems to be coupled to the protein local structure in the region encompassing aa 22 2 -22 8, which is distinct from the corresponding ... Moriguchi M (20 06) Crystalstructureof a major fragment of the salt-tolerant glutaminase from Micrococcus luteus K-3 Biochem Biophys Res Commun 346, 1118–1 124 Madern D, Ebel C & Zaccai G (20 00)...
... 5¢-aataatccatggggagtaatacttcctggcgta aaagtgaagtcc-3¢ and 5¢-attattggatccctattgctggtccgattctgccag-3¢ 3-TPR NlpI primers (residues 62 197) were 5¢-aataatccatgg gggcacagcttttatatgagcgcggag-3¢ and 5¢-aataatggatcctcactgttc cttatccgatttttcgaagtgc-3¢ ... Sciences and Division of Chemical Sciences, under Contract No DE-AC 02 98CH10886 X1 2C is supported principally by the Of ces of Biological and Environmental Research and of Basic Energy Sciences ... 25 5, 25 8, 25 9 ,26 1, 26 2, 26 4, 26 6 16 Helix 14 27 1, 27 5, 27 8 2. 45 Total 38 FEBS Journal 27 2 (20 05) 166–179 ª 20 04 FEBS C G M Wilson et al Crystalstructureof NlpI pendent domain One could therefore...