crystal structure of glycoprotein b from herpes simplex virus 1 217

... of < /b> doi: 10 .11 86 /14 23- 012 7 -17 -47 Cite this article as: Orosz et al., Involvement of < /b> p63 in the herpes simplex virus- 1- induced demise of < /b> corneal cells Journal of < /b> Biomedical Science 2 010 , 17 :47 ... HEp-2 cells by herpes simplex virus type J Virol 19 99, 73 :10 359 -10 370 Aubert M, Blaho JA: Modulation of < /b> apoptosis during herpes simplex virus infection in human cells Microbes Infect 20 01, 3:859-866 ... conceived of < /b> the study, performed research and revised the manuscript All authors read and approved the final manuscript Page of < /b> 10 11 12 13 14 15 16 17 18 19 Acknowledgements We thank Gyöngyi Ábrahám...

Ngày tải lên: 10/08/2014, 05:21

... IE-IIA IIB-IVB IIB-IVB IIA-IIB IVB-IVC Anx24(Ca32) AnxA5 Arg80-Glu99 Glu 113 -Arg2 71 CO 117 -Arg276 Asp39-Arg 118 Arg276-Asp280 – Glu 116 -Arg272 CO120-Arg277 Asp96-Arg1 21 Arg277–2 81 – Glu 112 -Arg2 71 – Asp92-Arg 117 ... Estimated from SCALEPACK output; b P 311 2 61. 05, 61. 05, 215 .36 40–2 .1 808320 27 412 10 0% (10 0%) 0.043 (0.433) 24054 (3750)/ 2656 ( 419 ) 4–3 21 2549 18 1/3 0. 219 (0.300)/ 0.280 (0.377) 51. 9 87.5 /11 .5 /1. 0 ... of < /b> cotton fiber cellulose synthase catalytic Ó FEBS 2003 2564 A Hofmann et al (Eur J Biochem 270) 12 13 14 15 16 17 18 19 20 21 22 23 24 25 subunits occurs via oxidation of < /b> the zinc-binding domains...

Ngày tải lên: 17/03/2014, 03:20

... residues 2 10 5, 11 4–289 and 303–562 of < /b> subunit A, residues 2 10 4 and 11 4–554 of < /b> subunit B, residues 2 10 4 and 11 6–556 of < /b> subunit C, residues 2 10 4 and 12 1–562 of < /b> subunit D, four molecules of < /b> ThDP, ... (2.32–2.26) 18 466 16 776 15 .8 (16 .3) 21. 4 (27.0) 0. 015 1. 477 92.5 21. 7 22.9 13 .4 15 .8 29.7 Neither the terminal residues, nor residues 10 5 11 3 in all subunits and residues 290–302 in one subunit, ... possibly stabilizing the binding of < /b> substrate An alternative pathway for substrate activation is favored by Baburina et al [10 12 ] and Li et al [13 ,14 ], who suggest that an activator molecule, bound...

Ngày tải lên: 30/03/2014, 10:20

... Corneaa TGb Gene 3d 10 d 30d 3d 10 d 30d CCR1 11 (9.2 12 ) 18 (13 –23) 20 (10 –26) (2.2–7 .1) 15 (9.0 19 ) (1. 7–7.0) CCR2 14 (9.2 19 ) 22 (11 –32) 14 (8 .1 24) (1. 5–4.2) 15 (11 19 ) (1. 3–4.4) CCR3 (1. 0–5.0) ... (2.2–20) (1. 8–4.7) CCR5 12 (12 .3 12 .5) 11 (8.0 14 ) 20 (8.9–36) (4.8 11 ) 57 (22 11 0) (7.0 10 ) CCR6 (2.4–3.0) (1. 0–2.5) (1. 5–8.5) (0.3 11 ) (1. 0–5.0) 14 (1. 0–40) CCR7 24 (8.0–40) (3.0–6.5) 17 (13 – 21) 13 ... MIP -1 Eotaxin -1 (3.2–3.3) (5.0 12 ) (5 .1 5.7) 17 (10 –24) 10 (7.0 13 ) (1. 5–3.9) (1. 3–8.8) (1. 0–4.4) (4.9–9.0) 68 (25 11 1) 35 (4.0–67) (1. 1–3 .1) (2.2–2.4) 0.7 (0.4 1. 0) (2.9–6.5) 20 (11 –28) (1. 0–7.0)...

Ngày tải lên: 18/06/2014, 22:20

... 2008, 5 :12 5 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 not essential for virus replication in cell culture J Virol 19 96, 70 :10 91- 1099 Kawaguchi Y, Van Sant C, Roizman B: Herpes simplex virus ... hyperphosphorylated by the protein kinase encoded by the U(L )13 gene of < /b> herpes simplex virus J Virol 19 98, 72 :17 31- 1736 Galvan V, Brandimarti R, Roizman B: Herpes simplex virus blocks caspase-3-independent ... 5 :12 5 http://www.virologyj.com/content/5 /1/ 125 Figure and construction of < /b> Strategy1 (see previous page) the recombinant virus MT1 01, 10 2, and 10 3 Strategy and construction of < /b> the recombinant virus...

Ngày tải lên: 20/06/2014, 01:20

... FEBS V Tiwari et al Nectin -1 mediates HSV -1 entry into RPE cells FITC stained CHO-HVEM RPE D Events B 11 4 A C 10 1 10 2 11 9 10 0 10 3 10 4 FITC stained CHO-nectin -1 Events RPE 10 0 10 1 10 2 FITC 10 3 10 4 ... susceptibility to infection by mutants of < /b> herpes simplex virus type 1, herpes simplex virus type 2, and pseudorabies virus Virology 246, 17 9 18 9 13 Shukla D, Liu J, Blaiklock P, Shworak NW, Bai X, ... for herpes simplex virus are heparan sulfate proteoglycans J Cell Biol 11 6, 12 73 12 81 Spillmann D (20 01) Heparan sulfate: anchor for viral intruders? Biochimie 83, 811 – 817 WuDunn D & Spear PG (19 89)...

Ngày tải lên: 18/02/2014, 14:20

... MN: Herpes simplex viruses and human herpes virus In Infectious Disease Edited by: Gorbach SL, Bartlett JG, Blacklow NR Philadelphia, PA: Saunders; 19 92 :16 67 -17 00 Sharma S, Mosunjac M: Herpes simplex ... prednisolone 15 mg daily and methotrexate mg weekly Her full blood count revealed a total count of < /b> 17 .5 × 10 9 with a neutrophilia of < /b> 10 .9 × 10 9, a C-reactive protein of < /b> 15 3 mg/litre, and an ESR of < /b> 75 ... Reactivation of < /b> herpes simplex virus type in the mouse trigeminal ganglion: an in vivo study of < /b> virus antigen and cytokines J Virol 19 99, 73(3) :17 67 -17 73 Minagawa H, Hashimoto K, Yanagi Y: Absence of < /b> tumour...

Ngày tải lên: 11/08/2014, 21:22

... (2 011 ) 19 44 19 54 ª 2 011 The Authors Journal compilation ª 2 011 FEBS T Yoshizawa et al 10 11 12 13 14 15 16 17 18 19 20 21 Robben J et al (2005) His374 of < /b> wheat endoxylanase inhibitor TAXI-I stabilizes ... 1. 0000 P 212 12 13 5.2 16 1.2 84.8 50.0 1. 90 720 554 13 8 568 6.5 (36.5) 95.6 ( 81. 0) 11 .3 (1. 9) 20 1. 91 130 634 6532 11 297 6 21 21. 1 25.9 0. 018 1. 782 87 .1 11. 1 1. 1 0.7 47.07 3AUP contained mostly legume ... Journal 278 (2 011 ) 19 44 19 54 ª 2 011 The Authors Journal compilation ª 2 011 FEBS 19 45 Structure < /b> of < /b> Bg7S, a XEGIP-like protein of < /b> soybean T Yoshizawa et al Fig Structure < /b> of < /b> Bg7S from soybean (A) Top...

Ngày tải lên: 14/03/2014, 23:20

... tetrasaccharide backbone (XXXG) For many years, endo -b- 1, 4-glucanases have been considered to be a subgroup of < /b> cellulases (EC 3.2 .1. 4) Recently, however, it has been clarified that some endo -b- 1, 4glucanases ... repeat of < /b> a seven-bladed beta-propeller domain in oligoxyloglucan reducing-endspecific cellobiohydrolase Structure < /b> 12 , 12 09 12 17 12 Ishida T, Yaoi K, Hiyoshi A, Igarashi K & Samejima M (2007) Substrate ... rate of < /b> 0.8 mLÆmin )1 References Vincken JP, York WS, Beldman G & Voragen AGJ (19 97) Two general branching patterns of < /b> xyloglucan, XXXG and XXGG Plant Physiol 11 4, 9 13 Fry SC (19 89) The structure...

Ngày tải lên: 23/03/2014, 05:22

... 0.05 18 ± 12 6 ± 0.08 9.5 ± 0.9 11 9 ± ) 0.09 12 2 ± 11 3.5 ± 0 .1 ) 0.03 13 2 ± 22 12 .1 ± 0.6 ) 0. 21 ND ND ) 0 .17 ND ND ) 0.02 45 ± 99 ± 0 .11 5.8 ± 0.6 87 ± ) 0.06 12 8 ± 3.5 ± 0 .1 ) 0 .18 ND ND ) 0 .14 ... 0 .14 ND ND 0.25 18 ± 19 3 ± 0.38 6.3 ± 1. 3 91 ± 0. 21 35 ± 8.3 ± 0.4 0.27 17 ± 2.0 ± 0 .1 0.09 16 8 ± 19 21 ± 0 .13 50 ± 16 2.6 ± 0.2 61 ± 2.7 ± 0 .1 FEBS Journal 278 (2 011 ) 2283–2295 ª 2 011 The Authors ... (2 011 ) 2283–2295 ª 2 011 The Authors Journal compilation ª 2 011 FEBS J P Kallio et al Crystal < /b> structure < /b> of < /b> a Thielavia arenaria laccase Fig (A, B) The substrate-binding pockets of < /b> (A) TaLcc1 and...

Ngày tải lên: 14/02/2014, 18:20

... (aapo ) are obtained from the apo imporc tin-a structure < /b> FEBS Journal 278 (2 011 ) 16 62 16 75 ª 2 011 The Authors Journal compilation ª 2 011 FEBS A V Mynott et al Crystal < /b> structure < /b> of < /b> importin-a:CLIC4 ... for auto-inhibitory regulation of < /b> NLS binding J Biol Chem 275, 212 18– 212 23 Kobe B (19 99) Autoinhibition by an internal nuclear localization signal revealed by the crystal < /b> structure < /b> of < /b> mammalian ... residues as a result of < /b> the binding of < /b> the CLIC4 NLS peptide FEBS Journal 278 (2 011 ) 16 62 16 75 ª 2 011 The Authors Journal compilation ª 2 011 FEBS 16 63 Crystal < /b> structure < /b> of < /b> importin-a:CLIC4 NLS peptide...

Ngày tải lên: 14/02/2014, 19:20

... (°) D165-M-H79 H79-M-H169 H79-M-wata Wata-M-H169 H169-M-D165 H 31- M-D165 H 31- M-watb a Fe 0.06 ± 0. 01 0.03 ± 0.02 0.04 ± 0.03 10 4.8 ± 1. 3 13 6 .1 ± 0.8 97.2 15 1.5 77 .1 74.5 11 1 .1 82 .1 169.5 11 8.8 ... 732659 12 46 31 6 .1 (5.2) 16 .6 (3.6) 18 .3 36 .13 1. 57 (1. 61 1. 57) 987 31 (6589) 19 .8 (33.7) ⁄ 23.6 (35.9) 0.008 1. 135 6984 29 630 31. 86 1. 35 (1. 38 1. 35) 15 29 91 (10 838) 18 .8 (23.9) ⁄ 20.3 (25.4) 0.008 1. 170 ... 71. 78 c = 76.85 b = 91. 81 50.0 1. 35 (1. 40 1. 35) 1. 94 4.8 (36 .1) 98.0 (97.0) 11 79 512 16 4370 7.3 (6.9) 15 .9 (5.8) 13 .8 a = 69.06 b = 71. 76 c = 76.40 b = 91. 72 50.0 1. 48 (1. 53 1. 48) 1. 92 7.7 (38.3)...

Ngày tải lên: 14/02/2014, 22:20

... FEBS 66 21 Crystal < /b> structure < /b> of < /b> human ACMSD 10 11 12 13 14 15 16 17 18 19 20 S Garavaglia et al compounds in K562 cells but not in normal human lymphocytes Biosci Biotechnol Biochem 64, 11 42 11 46 ... B Met 19 5 w1 w 268 DHAP Leu 296 w 51 C1 Trp 19 1 Phe 294 centre into a small pocket delimited by residues Asp2 91, Trp1 91, Met195, Arg47 and the Phe294Pro295-Leu296 amino acid stretch (Fig 3A ,B) ... Inhibition (%) 0.5 0 .1 0.5 0 .1 1 10 0 10 0 70 10 0 10 0 25 40 30 20 [ 21, 22], and insulin injection was observed to suppress such elevation Therefore, we propose that inhibition of < /b> hACMSD should be explored...

Ngày tải lên: 18/02/2014, 06:20

... soluble polypeptide chain of < /b> 234 amino acids (UniProt accession number Q81FP2) and has three homologues in B anthracis (str A2 012 ) (i.e bant_ 010 0 217 1, bant_ 010 04539 and bant_ 010 0 418 4) [4] All of < /b> ... conserved motif (Fig 7B) in the immediate neighborhood of < /b> the active site (His 110 , Pro 111 , Asp 112 , His 113 in the BcZBP numbering) adopts the same structural arrangement in both proteins, which ... Crystal < /b> structure < /b> of < /b> MshB from Mycobacterium tuberculosis, a deacetylase involved in mycothiol biosynthesis J Mol Biol 335, 11 31 11 41 10 Newton GL, Av-Gay Y & Fahey RC (2000) N-acetyl-1D-myo-inosityl-2-amino-2-deoxy-a-D-glucopyranoside...

Ngày tải lên: 19/02/2014, 00:20

... (%) 1. 8 C2 86.43 42.56 74.26 11 0 .19 10 814 0 30408 11 .4 (38 .1* ) 10 0 (10 0*) 6.0 (1. 9*) 11 .57 3.7 16 .0 19 .3 ˚ *Outer shell: 1. 9 1. 8 A gonal plates growing in bundles, and were up to ˚ 0.2 · 0 .1 · ... of < /b> intramolecular quenching Biochemistry 31, 6 011 –6 018 17 Gron H & Breddam K (19 92) Interdependency of < /b> the binding subsites in subtilisin Biochemistry 31, 8967–89 71 18 Larsen AN, Moe E, Helland ... residue, probably resulting from different scaffolds of < /b> the inhibitors Structures of < /b> PRK with inhibitors or sub- Fig Stereo plot illustrating inhibitor binding in subtilases The binding loop of < /b> chymotrypsin...

Ngày tải lên: 19/02/2014, 07:20

... areab (A2) ˚ Apolarc (A2) ˚ Buried surface areab (A2) ˚ Apolarc (A2) a 1IC6 1THM 38 24 ⁄ 14 23 38 18 ⁄ 20 23 30 15 ⁄ 15 15 (16 ⁄ 7) (10 ⁄ 13 ) (7 ⁄ 8) 10 15 2 87 23 262 10 11 5 4989 31 695 18 6 01 157 ... ˚ deviation (A) 1IC6–1THM 1SH7–1THM 2 81 279 2 61 (93%) 12 0 (43%) 0.84 279–279 238 (85%) 86 ( 31% ) 1. 21 2 81 279 246 (88%) 93 (33%) 1. 11 FEBS Journal 272 (2005) 832–845 ª 2005 FEBS regard to root ... 14 0.5 A ? ¼ 97.80° 13 5,690 37,893 93.2 (50.4) 9.3 (50.3) 13 .0 (2.7) 30 1. 84 (1. 88 1. 84) 14 .1( 22.6) ⁄ 19 .6(29.8) 0. 014 ⁄ 1. 5 21 13.3 ⁄ 25.4 ⁄ 34 .1 ⁄ 11 .9 89.9 ⁄ 9.9 ⁄ 0.2 a Rsym ¼ 10 0ÆShSi|Ii(h) –...

Ngày tải lên: 19/02/2014, 16:20

... 10 16 Brandl, M & Lindow, S.E (19 96) Cloning and characterization of < /b> a locus encoding anindolepyruvate decarboxylase involved in 2320 A Schutz et al (Eur J Biochem 270) ¨ 10 11 12 13 13 a 14 15 ... of < /b> thiol groups of < /b> pyruvate decarboxylase from brewer’s yeast Biomed Biochim Acta 47, 9 18 46 Baburina, I., Gao, Y., Hu, Z., Jordan, F., Hohmann, S & Furey, W (19 94) Substrate activation of < /b> brewers’ ... 745–7 51 34 Killenberg-Jabs, M., Konig, S., Eberhardt, I., Hohmann, S & ¨ Hubner, G (19 97) Role of < /b> Glu 51 for cofactor binding and cata¨ lytic activity in pyruvate decarboxylase from yeast studied by...

Ngày tải lên: 20/02/2014, 11:20

... (99.6) 14 7 220 46 819 99.5 (98.6) 11 .1 54.6 6 .1 3.6 (3.6) 12 .4 49.2 8.2 3.7 (3.7) 11 .9 35.6 8.7 3.5 (3.5) 13 .1 41. 2 5.7 3 .1 (2.8) 11 8.08 14 2.25 74.25 90 10 4 .1 90 42 .1 117 .70 14 1.39 75.23 90 10 4.5 ... coordinate by Luzzati (A) N BL-6A T AR-NW12A G AR-NW12A TG BL-6A 2.3 10 .9 (2.2) 2.5 11 .0 (2 .1) 2.6 9 .1 (2.8) 2.4 8.7 (1. 8) 18 9 811 52 6 71 100 (99.9) 14 9 207 40 4 51 98.4 (98.0) 12 5 11 1 35 920 98.2 ... 52.8 11 7.45 14 1.62 75 .15 90 10 4.0 90 58.0 11 8.36 14 1.20 76 .12 90 10 5.4 90 41. 0 F 19 .9–2.3 52 579 0.247 0.2 91 TG 19 .9–2.5 40 379 0. 217 0.258 N 19 .9–2.6 35 873 0.2 71 0.309 N 19 .9–2.4 46 7 41 0.222...

Ngày tải lên: 06/03/2014, 09:22

... P 21 2.2 46 203 14 .1 (2 .1) 98.3 (95.5) 3.5 (2.2) a ¼ 62.02 b ¼ 10 3.87 c ¼ 80. 51 b ¼ 10 1.83 0.058 (0. 41) 0.209 0.279 P 212 1 21 2.4 36 088 10 .1 (1. 1) 98.0 (96.5) 2.5 (2.3) a ¼ 83. 81 b ¼ 97.94 c ¼ 12 2 .16 ... purified enzyme was crystallized in two crystal < /b> forms, characterized by space groups P 21 and P 212 1 21 The structure < /b> was solved by molecular replacement (molrep) [17 ] The structure < /b> of < /b> both crystal < /b> forms ... rmsd, respectively Crystal < /b> form (P 212 1 21) Subunita Crystal < /b> form (P 21) A B C D A B C D 0.9 31 0.368 0.274 0.402 0.739 0.498 0.370 0.3 81 3.937 3.705 3 .17 5 0.534 3.335 3 .13 3 2.253 2.8 41 a The rmsds were...

Ngày tải lên: 16/03/2014, 11:20