Metabolic Engineering Of Saccharomyces Cerevisiae For Sesquiterpene Production Gionata Scalcinati

... 5¢-CACACTACACTGGGAAGCAGAGACTCCAGC-3¢ and 5¢-AGCTGGCCCAGCAGCCATACACAGTTAAAG3¢ The cDNA was subcloned into the EcoRV site of pBluescript SK(+) (Stratagene, La Jolla, CA, USA) and sequenced using an automated ... Na -palmitoylation of Gas is regulated also remains unclear However, we assume that mammalian Gup1 competes with Skn for Shh to prevent palmitoylation rather than catalyzing...

Ngày tải lên: 18/02/2014, 16:20

... properties and cell size to the presence of benzoic acid Transient response to benzoic acid total membrane surface area (=AXÆCXÆVL) available for the benzoic acid transport during the transient ... al Transient response to benzoic acid A B C D E Fig Transient responses to the shift in benzoic acid concentration (the timing of the shift is marked b...

Ngày tải lên: 07/03/2014, 04:20

... the most reliable means of purifying integral membrane proteins from the mitochondrial outer membrane, and of the 11 most abundant integral proteins, 10 had a-helical transmembrane segments Of ... the proteins of the outer membrane, we set out to determine what proportion was integral and to identify the major protein species To determine whether the...

Ngày tải lên: 19/02/2014, 07:20

... Application of the antibody to thin sections of wild-type cells grown on oleic acid medium Fig SKL is required to direct Mls1p to the peroxisomes under oleic acid- medium conditions (A) Speci®city of the ... issue of the partitioning of the glyoxylate cycle in cells grown under fatty acid- medium conditions has hitherto remained open We showed h...

Ngày tải lên: 22/02/2014, 04:20

... certain pools of fatty acids To assess the role of acyl-CoA synthetases in regulating particular pools of fatty acids, we used mutants of S cerevisiae characterized by various combinations of deletions ... exogenous fatty acids, additional release of internal fatty acids had to contribute to the increase of intracellular fatty acids However, ana...

Ngày tải lên: 07/03/2014, 05:20

... (PLS) regressions For future metabolic engineering of strains with the purpose of obtaining increased NADPH levels and for the understanding of the regulation of the NADPH level it is important ... acid-producing mycelia [26] Overproduction of 6PGDH resulted in an increase of synthesis of pyridine nucleotide cofactors, as the total pool of these increase...

Ngày tải lên: 07/03/2014, 17:20

... AACEVAPD1 AACEVAPD3 AACEVAPD2 AACEVAPD5 AACEVAPD4 AACEVAPD6 Vma3p Vma11p Vma16p 100 97 80 80 80 80 50 50 32 100 94 93 93 54 51 28 AACEVAPD5 AACEVAPD2 AACEVAPD3 AACEVAPD1 Table Alignments of the ... R Hirata of the Institute of Physical and Chemical Research (Wako, Japan), and the antibody against the 100-kDa (a) subunit of S cerevisiae V-ATPase was purchased from Mol...

Ngày tải lên: 08/03/2014, 23:20

... PAHs are orthologs of yeast PAH1 and mammalian lipins Based on previous studies demonstrating the involvement of yeast PAH1 and mammalian lipins in storage lipid accumulation [27], we were initially ... derepression of key enzymes involved in phospholipid (PL) biosynthesis [20,21] In mammals, highly phosphorylated forms of Lipin-1 are enriched in the cytoplasm, where...

Ngày tải lên: 15/03/2014, 00:20

... investigate patterns of flux control The model with the best-fit Vmax parameters was used as the base model for parameter scanning using routines contained in GEPASI METHODS Model A model of branched glycolysis, ... in all combinations) using the parameter scanning functions of GEPASI Only around 50% of the simulations reached steady state, and of those that did...

Ngày tải lên: 17/03/2014, 11:20

... either are involved in oligomerization of the Mrs2p channel protein or in forming structures participating in the gating of this channel Possibly they contribute to both functions The coiled-coil ... formation of higher order structures of the Mrs2p part on the inner side of the membrane, which may contribute to the proposed opening ⁄ closing of the ch...

Ngày tải lên: 23/03/2014, 10:21

... al Dissociation of tubulin H+-ATPase complex by glucose H+-ATPase activity of isolated plasma membrane is inhibited by tubulin Plasma membranes isolated from S cerevisiae were incubated in the ... hydrophobic tubulin partitioning into the detergent phase (HAT) is the acetylated tubulin forming a complex with H+-ATPase The finding of the H+-A...

Ngày tải lên: 23/03/2014, 11:20

... F99L kinase, suggests a mechanism of co-ordinated feedback regulation of the synthase and the kinase Arginine feedback inhibition of N-acetylglutamate kinase is altered in the absence of N-acetylglutamate ... expressing the kinase protein) allow growth of the argininedeficient mutant in the absence of arginine On the other hand, plasmids pYK...

Ngày tải lên: 23/03/2014, 21:20

... containing galactose as a carbon source Wild-type and mrg19 strains grew on galactose plates within 12 h while gal3 strain showed the characteristic delay in growth on galactose Interestingly, the ... disruption of MRG19 might effect GAL induction only at low galactose concentrations This hypothesis was tested by monitoring galactokinase induction as a function of...

Ngày tải lên: 31/03/2014, 08:20

... references isc1D + pDZ6 was a transformant of the isc1D mutant with the ISC1 containing plasmid, pDZ6 cell homogenate The characteristics of the Disc1::HIS5 deletion strain, DZY1, which was constructed ... sphingolipids in the heat stress response of Saccharomyces cerevisiae J Biol Chem 272, 32566–32572 14 Wells, G.B., Dickson, R .C & Lester, R.L (1998) Heat-induced elevation of...

Ngày tải lên: 31/03/2014, 09:20

Ngày tải lên: 21/06/2014, 06:20