Proteomic analysis of saccharomyces cerevisiae KAY446 under very high gravity conditions

... affinity (lower KDapp) WW WW WW WW (c) X WW WW Figure for protein ligands A model10 the optimization of interactions between WW domains and A model for the optimization of interactions between WW domains ... suggesting that WW domains have sufficient recognition malleability to bind structurally similar peptide ligands within the PY (PPxY) and LPxY ligand classes Bot...

Ngày tải lên: 14/08/2014, 16:21

... either are involved in oligomerization of the Mrs2p channel protein or in forming structures participating in the gating of this channel Possibly they contribute to both functions The coiled-coil ... formation of higher order structures of the Mrs2p part on the inner side of the membrane, which may contribute to the proposed opening ⁄ closing of the ch...

Ngày tải lên: 23/03/2014, 10:21

... molecules in the course of hypoxia-induced apoptotic pathways Such analysis of trophoblastic cells will, in the near future, yield insights into the mechanisms of preeclampsia and other hypoxia-related ... and by modulating phosphorylation of JNK In conclusion, various pro- and antiapoptosis-related proteins were expressed in the process of hypoxia-induced apoptos...

Ngày tải lên: 31/10/2012, 15:12

... Modeling of wind turbine The turbine is a wind energy conversion system It consists of an induction generator and other electrical control power requirements The wind turbine is effected by many parameters ... losses in wind turbine, ρ = air density in kg/m3, A = swept area in m2, Cp =power coefficient of wind turbine, V = wind speed in m/s Mechanical power of w...

Ngày tải lên: 05/09/2013, 16:10

... overexpressing a- synuclein A proteomic analysis is expected to identify cellular alterations that are associated with dopamine treatment and modulated by a- synuclein overexpression, without any a priori ... eukaryotic initiation factor 5A (eIF 5A) , parathymosin, L7 ⁄ L12, annexin A2 , annexin A5 , aldolase A, fascin and peroxyredoxin 1] displayed quantitative diff...

Ngày tải lên: 15/02/2014, 01:20

... 5¢-CACACTACACTGGGAAGCAGAGACTCCAGC-3¢ and 5¢-AGCTGGCCCAGCAGCCATACACAGTTAAAG3¢ The cDNA was subcloned into the EcoRV site of pBluescript SK(+) (Stratagene, La Jolla, CA, USA) and sequenced using an automated ... Na -palmitoylation of Gas is regulated also remains unclear However, we assume that mammalian Gup1 competes with Skn for Shh to prevent palmitoylation rather than catalyzing...

Ngày tải lên: 18/02/2014, 16:20

... the most reliable means of purifying integral membrane proteins from the mitochondrial outer membrane, and of the 11 most abundant integral proteins, 10 had a-helical transmembrane segments Of ... the proteins of the outer membrane, we set out to determine what proportion was integral and to identify the major protein species To determine whether the...

Ngày tải lên: 19/02/2014, 07:20

... Application of the antibody to thin sections of wild-type cells grown on oleic acid medium Fig SKL is required to direct Mls1p to the peroxisomes under oleic acid- medium conditions (A) Speci®city of the ... issue of the partitioning of the glyoxylate cycle in cells grown under fatty acid- medium conditions has hitherto remained open We showed h...

Ngày tải lên: 22/02/2014, 04:20

... properties and cell size to the presence of benzoic acid Transient response to benzoic acid total membrane surface area (=AXÆCXÆVL) available for the benzoic acid transport during the transient ... al Transient response to benzoic acid A B C D E Fig Transient responses to the shift in benzoic acid concentration (the timing of the shift is marked b...

Ngày tải lên: 07/03/2014, 04:20

... certain pools of fatty acids To assess the role of acyl-CoA synthetases in regulating particular pools of fatty acids, we used mutants of S cerevisiae characterized by various combinations of deletions ... exogenous fatty acids, additional release of internal fatty acids had to contribute to the increase of intracellular fatty acids However, ana...

Ngày tải lên: 07/03/2014, 05:20

... AACEVAPD1 AACEVAPD3 AACEVAPD2 AACEVAPD5 AACEVAPD4 AACEVAPD6 Vma3p Vma11p Vma16p 100 97 80 80 80 80 50 50 32 100 94 93 93 54 51 28 AACEVAPD5 AACEVAPD2 AACEVAPD3 AACEVAPD1 Table Alignments of the ... R Hirata of the Institute of Physical and Chemical Research (Wako, Japan), and the antibody against the 100-kDa (a) subunit of S cerevisiae V-ATPase was purchased from Mol...

Ngày tải lên: 08/03/2014, 23:20

... PAHs are orthologs of yeast PAH1 and mammalian lipins Based on previous studies demonstrating the involvement of yeast PAH1 and mammalian lipins in storage lipid accumulation [27], we were initially ... derepression of key enzymes involved in phospholipid (PL) biosynthesis [20,21] In mammals, highly phosphorylated forms of Lipin-1 are enriched in the cytoplasm, where...

Ngày tải lên: 15/03/2014, 00:20

... investigate patterns of flux control The model with the best-fit Vmax parameters was used as the base model for parameter scanning using routines contained in GEPASI METHODS Model A model of branched glycolysis, ... in all combinations) using the parameter scanning functions of GEPASI Only around 50% of the simulations reached steady state, and of those that did...

Ngày tải lên: 17/03/2014, 11:20

... al Dissociation of tubulin H+-ATPase complex by glucose H+-ATPase activity of isolated plasma membrane is inhibited by tubulin Plasma membranes isolated from S cerevisiae were incubated in the ... hydrophobic tubulin partitioning into the detergent phase (HAT) is the acetylated tubulin forming a complex with H+-ATPase The finding of the H+-A...

Ngày tải lên: 23/03/2014, 11:20