Isolation, cloning and characterization of the FSH beta gene promoter of the chinook salmon (oncorhynchus tshawytscha)

... (2008) A novel plant protein disulfide isomerase family homologous to animal P5 – molecular cloning and characterization as a functional protein for folding of soybean seed-storage proteins FEBS J ... both luminal proteins Generally, PDI family proteins play important roles in folding and quality control of nascent polypeptides [25] In soybean cotyledon, lar...

Ngày tải lên: 18/02/2014, 11:20

... 5¢-GTATCAGCTTC(C ⁄ T)TCAAATGTC-3¢ 5¢-CCATCACAAGAAACTAGAGAAAC-3 5¢-TTAAGTACTATAAAT-ACACAGCCTA-3¢ 5¢-CGAGCTCACTGCCTCTCAAC-3¢ 5¢-ACTCGTAGC-ACAGAGACAGAG-3¢ 5¢-ATGGCAGTAGTAGCAG-CTCC-3¢ 5¢-TCAGGTGTCT-AAGTTCAGAGATTC-3¢ ... Remarks 10 11 12 13 5¢-GG (A ⁄ T)CA (A ⁄ C)GG (A ⁄ T)AC (A ⁄ C)TT(C ⁄ T)GC(C ⁄ G ⁄ T)AAGGT-3¢ 5¢-ACAAA (A ⁄ C )A( A ⁄ G) (A ⁄ G ⁄ T )A( C ⁄ T) (A ⁄ C ⁄ G)ACACCACAAGACC)3¢ 5...

Ngày tải lên: 19/02/2014, 07:20

... vivo state of rainbow trout HSF1, however, it remains to be elucidated whether the HSF1 isoforms of rainbow trout form heterotrimers in vivo Why are there two isoforms of HSF1 in rainbow trout? Although ... of the heat shock response and beyond FASEB J 15 , 11 18 11 31 Mosser, D.D., Heikkila, J.J & Bols, N.C (19 86) Temperature ranges over which rainbow tr...

Ngày tải lên: 19/02/2014, 12:20

... process of soybean seeds as decreases in the mRNA levels of GmPDIL-1, GmPDIS-1 [26] and GmPDIM [27] were observed during the accumulation of the storage proteins Expression of certain seed storage proteins ... (2008) A novel plant protein disulfide isomerase family homologous to animal P5: molecular cloning and characterization as a functional protein f...

Ngày tải lên: 07/03/2014, 05:20

... (S)-cheilanthifoline, not (S)-nandinine [7,30,31] If we assume that (S)-nandinine is produced in E californica, there may be no need for a catalyst from (S)-nandinine to (S) -stylopine However, nandinine could ... 1029 Stylopine synthase from Eschscholzia californica N Ikezawa et al E californica [27] Indeed, CYP80B1 has been cloned using this strategy, although other P450s invol...

Ngày tải lên: 07/03/2014, 10:20

... product of the second round of PCR was cloned and the 1066 bp sequence encoding 125 amino acids of the A-chain, the 19 amino acids linker, and 216 amino acids of the B-chain of the ml gene was obtained ... (Met153 of the ML1p and ML2p B-chains and Met156 of the ML3p B-chain corresponding to the ricin B-chain Leu152; and Met234 of the ML1p and...

Ngày tải lên: 07/03/2014, 15:20

... glabra GgbAS1 b-amyrin synthase [6], was prepared by PCR using GgbAS1 as a template, Taq DNA polymerase (Takara Shuzo, Kyoto, Japan), the primers 50 -GAAGCATA TCCACTATGAAGATGA-30 and 50 -TGAATACTCCCGTG ... Ebizuka, Y (2001) Cloning and characterization of a cDNA encoding b-amyrin synthase involved in glycyrrhizin and soyasaponin biosynthesis in licorice Biol Phar...

Ngày tải lên: 08/03/2014, 23:20

... nucleotide sequence of oyster CaLP cDNA obtained by RACE, a PCR reaction was performed using a pair of specific primers P3 (5¢-GGAAGAATACAGACACGGACAG-3¢) and P4 (5¢-ATAACAACAGTTTATACATCGCTTC-3¢) corresponding ... metabolism and calcium signaling pathways Experimental procedures RNA preparation and cDNA synthesis Adult specimens of P fucata were purchased from Guofa P...

Ngày tải lên: 16/03/2014, 23:20

... hydrochloride, and mM EDTA in a volume of 0.5 mL Three microliters of 4-vinylpyridine were then added, and alkylation was allowed to proceed for h at room temperature The S-pyridylethylated proteins ... and cloning of tigrin and ablomin During the isolation process of a prothrombin activator from the Duvernoy’s gland of Yamakagashi snake (R tigrinus tigrinus), we ident...

Ngày tải lên: 18/03/2014, 01:20

... expression of L schmitti CHH gene The size and expression of the CHH mRNA in different tissues were determined by northern blot analysis of total RNA isolated from eyestalk, muscle and stomach ... interference RNA in vivo, for example, the length of target mRNA, the length and concentration of dsRNA, the region of homology between the dsRNA and t...

Ngày tải lên: 23/03/2014, 10:20

... identity with HvTrxh1 and HvTrxh2 are present in the TIGR barley sequence database As only HvTrxh1 and HvTrxh2 have been identied so far in the barley seed proteome, the other thioredoxin h isoforms ... endosperm and embryo show in detail how HvTrxh1 and HvTrxh2 are distributed in these tissues HvTrxh1 from all three tissues is observed The relative intens...

Ngày tải lên: 23/03/2014, 17:22

... human and a murine NICN1 cDNA clone from the IMAGE collection and determined the complete sequences of these cDNAs The comparative analysis of the human, canine, and murine NICN1 cDNA revealed a ... CACCAGgtcagctgggcctca 423 418 bp (exon 3, 11 4 bp) … CCAAAGgcaagtgactttgca 4 01 bp (exon 4, 72 bp) 495 … CTTGAGgtaagctctctaaca 3 71 bp (exon 5, 10 5 bp) 600 … TTCGACgtgag...

Ngày tải lên: 23/03/2014, 21:20

... step and to elute the recombinant enzyme in the elution step were determined in preliminary experiments Fractions were analysed for epoxide hydrolase activity and protein content based on the ... probably corresponding to the histidine and tyrosine residues, respectively [6–8] Several compounds were tested for potential inhibitory effects on the hydrolysis of TSO...

Ngày tải lên: 31/03/2014, 08:20

... II Isolation and Characterization of the Promoter of the FAT10 Gene 32 2.3.2 The responsive domain of the FAT10 promoter to TNF-α and IFN-γ resides in the region upstream of FAT10 promoter To ... CLONING AND CHARACTERIZATION OF THE PROMOTER OF THE CANCER- ASSOCIATED GENE, FAT10 ZHANG DONGWEI (M Sc.) Wuhan Institute of Virology Chine...

Ngày tải lên: 03/10/2015, 20:57

... the mechanism of transcriptional regulation of the FSH subunit gene promoter of the Chinook salmon The Chinook salmon FSH subunit gene and 5’ flanking sequence will be isolated, sequenced and ... regard to the size of introns and 3’ untranslated region (3’ UTR; Figure 3) Compared to the 0.3 – 0.35 kb size of intron of the LHβ genes, the size...

Ngày tải lên: 08/11/2015, 17:15