INTERACTION OF MITOCHONDRIAL SSC1 DOMAINS WITH n TERMINAL DOMAIN OF TIM44

... Bhagwat SV, Biswas G, Anandatheerthavarada HK, Addya S, Pandak W & Avadhani NG (199 9) Dual targeting property of the N-terminal signal sequence of P450 1A1 Targeting of heterologous proteins to ... open N-terminal signal domain is critical for protein targeting to the ER (Fig 2D) In contrast to the targeting of CYP 1A1 requiring N-terminal truncation, w...

Ngày tải lên: 18/02/2014, 16:20

... L25NMe monomer to the CXCR1 N -domain peptide We used ITC to determine the enthalpy (DH), entropy (DS), and the free energy (DG) of binding of monomeric IL-8 to the receptor CXCR1 N -domain The binding ... essential for interleukin-8 (IL-8) binding to CXCR1 receptor J Biol Chem 279, 36175–36178 Rajagopalan L & Rajarathnam K (2004) Ligand selectivity and affini...

Ngày tải lên: 19/02/2014, 05:20

... revealed that the N-terminal region of the bacterial replicative polymerase PolC includes two structural domains: PolC- NI and PolC- NII Both domains are distantly related to domain V of the DNA ... N-terminal region has two adjacent structural domains, both related to PolIIIs -V We termed these two putative domains PolC- NI and PolC- NII (Fi...

Ngày tải lên: 05/03/2014, 23:20

... Role of JNK in radiation- induced mitochondrial cell death M.-J Kim et al Fig Ionizing radiation induces expression of Fas and activation of caspases in human cervical cancer cells (A) Ionizing radiation- induced ... development of molecular targets for cancer treatment Results To examine the kinetics of the apoptotic cell death induced by ionizi...

Ngày tải lên: 07/03/2014, 05:20

... (20 04) Unanticipated antigens: translation initiation at CUG with leucine PLoS Biol 2, e366 15 Kozak M (1986) Point mutations dene a sequence anking the AUG initiator codon that modulates translation ... an N-terminal leucine amino acid Our present study indicates that nonAUG translation initiation may be operable more often than anticipated This may have a grea...

Ngày tải lên: 07/03/2014, 10:20

... effect on the fibrillation properties of the most extreme disease-associated extension of seven additional alanines, fibrillation kinetics and fibril properties of N-WT were compared with those of the ... reason, fibril formation was analyzed with the N-terminal domain of PABPN1 consisting of amino acids 1–125 of the wild-type protein (N-WT) Beca...

Ngày tải lên: 07/03/2014, 11:20

... suitable for discriminating the cytotoxic effect of < /b> the stefin < /b> prefibrillar forms In order to determine whether the prefibrillar aggregates of < /b> stefin < /b> B exert their toxic effect via lipid membrane interactions, ... 2005 FEBS Stefin < /b> B and cellular toxicity to its homolog, stefin < /b> A [17,18] By following the kinetics of < /b> fibril formati...

Ngày tải lên: 07/03/2014, 17:20

... expressed GAF domains, e.g the cyclic nucleotidebinding affinities of PDE2 and )5 GAF domains However, we were able to determine additional GAF domain properties because of the dissociation of the allosteric ... regulating the cGMP affinity of the PDE11A4 tandem GAF domain and thus allosterically affect PDE11 activity The role of the N-terminus of the...

Ngày tải lên: 16/03/2014, 06:20

... representation of the binding of the N-terminal domain (ND) of PEX1 to the peroxisomal membrane PEX1 is predicted to interact with the PEX6 portion of AAA domains PEX1- ND binds to the peroxisomal ... vivo [22] On the other hand, colocalization of VCP and PtdIns-4-kinase IIa on the buoyant subfraction of ER-derived membrane was observed [66] The ND of...

Ngày tải lên: 16/03/2014, 12:20

... six cysteines in yeast Erv1p was the major goal of this paper All three pairs of cysteines in Erv1p are indispensable for in vivo activity The six cysteines of yeast Erv1p can be grouped into ... any activity in vitro or in vivo This phenotype is in agreement with the finding that the C130–C133 pair is part of the primary redox- acti...

Ngày tải lên: 17/03/2014, 10:20

... additional N -domain, homologous to the N-domains of ClpA or ClpB, and a linker domain homologous to, but half the size of, the linker domain of ClpB [19] The N-terminal region contains two 32-amino acid ... tuberculosis ClpC1 has an inherent ATPase activity and also functions like a chaperone in vitro Furthermore, we investigated the role of the N-termin...

Ngày tải lên: 23/03/2014, 06:20

... alteration of the protein wherein potential lipid binding sites may be exposed Thus, it appears that helix 1¢ plays a structural role, serving to maintain the integrity of the helix bundle in the absence ... changes in the N-terminal domain of apolipoprotein E J Lipid Res 40, 93–99 10 Fisher CA, Narayanaswami V & Ryan RO (2000) The lipid associated conforma...

Ngày tải lên: 30/03/2014, 11:20

... expanded HCII levels in the Table Recombinant heparin cofactor II (HCII) variants investigated in this study wt-rHCII, wild-type recombinant heparin cofactor II Variant Sequence wt-rHCII DC DC/F195C ... of the inhibitor’s N-terminal extension, compatible with a permissive mode of Ó FEBS 2004 N-terminal domain of heparin cofactor II (Eur J Biochem 271) 4281 Ta...

Ngày tải lên: 30/03/2014, 15:20

... binding of NCp7 to the C-terminal domain (80–96 )Vpr [8] may expose the N-terminal (1–39) portion of the protein and allow its tight binding to Lys-tRNA synthetase [15] The C-terminal domain (52–96 )Vpr ... when comparing the isolated domains of (1–51 )Vpr [25] and (52–96 )Vpr [26] with the intact Vpr protein The differences in structure are loc...

Ngày tải lên: 31/03/2014, 23:20

... domains and N terminal domain of Tim44 and study their interactions. We found  that the N terminal domain of Tim44 adopts a disordered structure and the  nucleotide binding domain and peptide binding domain of Ssc1 adopt well folded  ... about the structure of the complex of Tim44 Ssc1:  The N terminal of Tim44 has its binding sites on both NBD and PBD of Ssc1,  probably in a way that the ...

Ngày tải lên: 08/11/2015, 16:31