Kinectin in protein synthesis and membrane dynamics

... further investigate the role of the SEA domain in cleavage of membrane-tethered mucins we carried out site-directed mutagenesis of the FRPG ⁄ SVVV site and evaluated hybrid mucins in which the SEA ... previously The fragments were then cloned into the SpeI ⁄ XhoI sites of the polylinker in MUC1FDTR The remaining portions of the polylinker intr...

Ngày tải lên: 19/02/2014, 18:20

... a-helices of the N-terminal 185 residues of the functional domain of the HA2 subunit of the in uenza virus hemagglutinin protein and to detect conformational heterogeneity of the protein within IBs ... use of molecular and chemical chaperones and modu- Potential of bacterial inclusion bodies lation of the expression conditions to reduce the...

Ngày tải lên: 06/03/2014, 00:20

... sources or Triacylglycerol mobilization in yeast as sinks for internal fluxes From a biological point of view, they allow the cell to dispose of excess metabolites by storing them in an inert form When ... inoculation of cells into fresh culture medium; and, most importantly, (c) yields a quantitative description of the utilization of TAG depots for the production o...

Ngày tải lên: 16/03/2014, 04:20

... that the poly (b-lysine) chain of nourseothricin must be synthesized by an NRPS-like system in a mechanism that uses NspA, a stand-alone b-lysine adenylating enzyme (A- domain) and NspB, a b-lysine ... b-lysines are added to the gulosamine moiety By analogy to some poly amino acids such as folylpoly c-glutamate, an amino-acid polymer produced by bacteria and eukary...

Ngày tải lên: 17/03/2014, 17:20

... study the roles of stress-activated signalling pathways on cell function We find that these stresses rapidly and profoundly inhibit protein synthesis and markedly alter the phosphorylation and/ or ... inhibition of protein synthesis, it will be important to consider their effects on protein synthesis when studying the effects of these stress conditions on ce...

Ngày tải lên: 18/03/2014, 01:20

... bridging the A and G b-strands of the I27 protein during the main unfolding barrier.[39] To further validate this view and gain insight into the role of solvent hydrogen bonds in protein unfolding, ... separating b-strands In Figure B, we define the pulling coordinate for the A and G b-strands as the distance between the first amino acid of strand...

Ngày tải lên: 22/03/2014, 18:20

... complimentary mutagenic primers (IDS1 2A, 5¢CGATGGAAGAAGGTGCTGAGAATTCGAAGC-3¢ and IDS12B, 5¢-GCTTCGAATTCTCAGCACCTTCTTCCA TCG-3¢) were synthesized and purified by MWG-Biotech AG (Ebersberg, Germany) ... spectropolarimeter (Jasco UK, Great Dunmow, UK) The bandwidth was nm and the scanning speed was 200 nmÆmin)1 with a response time of s and a data pitch of 0.5 nm Typically, 16 sp...

Ngày tải lên: 23/03/2014, 10:21

... Metathesis-based Monoliths in Catalysis 364 Grafted Supports for Ring-closing Metathesis (RCM) and Related Reactions 364 Poly-(N,N-dipyrid-2-yl-7-oxanorborn-2-en-5-ylcarbamido·PdCl2)-grafted Monolithic ... ISBN 3-5 2 7-3 063 0-7 V To Andrea Polymeric Materials in Organic Synthesis and Catalysis Edited by Michael R Buchmeiser Copyright © 2003 WILEY-VCH Verlag GmbH &...

Ngày tải lên: 03/04/2014, 12:15

... replication include protein 3AB, the precursor of 3A, which is a small membrane binding and RNA binding protein, the terminal protein VPg, RNA polymerase 3Dpol and proteinase 3Cpro/3CDpro As a proteinase ... absence of a viral RNA template (Figs 2B and 2C, lane 3) When 3CDpro mRNA, containing the R84S/I8 6A mutations in the RNA binding domain of 3Cpro, wa...

Ngày tải lên: 19/06/2014, 08:20

... binders) and C (4-wt.% YF3 + 3-wt.% CaF2 and 4.5-wt.% organic binders) sintered at 1750ºC for 2h after de-waxing in Air 228 Advances in Ceramics - Synthesis and Characterization, Processing and ... carbon during binder burnout and sintering of tape-cast aluminium nitride J Am Ceram Soc., Vol.76, No.1, pp 166– 172 242 Advances in Ceramics - Synthesis and Ch...

Ngày tải lên: 29/06/2014, 13:20

... current and initial weights of the sample, K and n are the constant coefficients, and τ is the holding time 16 Advances in Ceramics - Synthesis and Characterization, Processing and Specific ... Phase composition and physical and mechanical properties of the synthesis products in the Ti–Cr–Al–C system 12 Advances in Ceramics - Synthesis and Character...

Ngày tải lên: 29/06/2014, 13:20

... L-3,4dihydroxyphenylalanine (l-dopa) by tyrosine hydroxylase (TH) and l-dopa is converted into cytosolic dopamine (cda) by aromatic amino acid decarboxylase (AADC) Cytosolic dopamine is transported into the ... understand the many homeostatic mechanisms involved in dopamine synthesis, release and reuptake We have demonstrated that substrate inhibition of tyrosine hydroxylase by...

Ngày tải lên: 13/08/2014, 16:20

... ratio in the cell at the steady state In this way, the rates of the irreversible and independent mechanisms of synthesis and degradation are joined and balanced both in the first instance and subsequently ... protein on its own rate of synthesis any more likely Once manufactured, the new protein is released from the synthetic machinery of the ribosome into...

Ngày tải lên: 13/08/2014, 16:20

... ATP-dependent kinesin motor protein, making its activity controllable 1.3.4 Kinectin: A multifunctional protein that interacts with kinesin Kinectin, an integral membrane protein found in ER (Kumar ... dynamic anchors, the kinectin- kinesin interaction appears most prominent Kinectin interacts with kinesin and the kinesin-binding domain enhances the microtubule-stimulated kine...

Ngày tải lên: 16/10/2015, 15:38