Molecular characterization and developmental expression patterns of the zebrafish twist gene family

Tài liệu Báo cáo khoa học: Molecular characterization, phylogenetic relationships, and developmental expression patterns of prion genes in zebraﬁsh (Danio rerio) doc

... of mammalian origin by the ﬁsh intestine Comparison of zebraﬁsh prp1, prp2, and prp3 developmental expression patterns The developmental expression patterns of prp1 and prp2 coding for long zebraﬁsh ... distributed within the CNS 509 Expression of prion genes in the developing zebraﬁsh and in speciﬁc areas outside the CNS of the developing zebraﬁsh The prp1 and prp2-expressing sites in the developing ﬁsh ... Analysis of the N-terminal repeat domain of zebraﬁsh PrP1 and PrP2 The N-terminal domain of zebraﬁsh PrP1 contained nine repeats (residues 53–332) including four highly conserved 37 amino acid-long...

Báo cáo khoa học: High and complementary expression patterns of alcohol and aldehyde dehydrogenases in the gastrointestinal tract Implications for Parkinson’s disease docx

... systems in the epithelial lining of the GI tract deserve further investigation as to their possible etiological roles for forms of PD Here we map the expression pattern of the Adh1, Adh3, Adh4 and ... in the epithelial cells of the gastric pits and in the neck of the gastric glands Aldh1 expression continued in duodenum with the signal conﬁned to the epithelial cells of the villi, whereas the ... Results The distribution and relative intensity of expression of the Adh1, Adh3, Adh4 and Aldh1 genes in the GI tract of rats and mice is summarized in Fig and Table Figures and document key ﬁndings...

Báo cáo khoa học: Molecular cloning and functional expression of the human sodium channel b1B subunit, a novel splicing variant of the b1 subunit potx

... characterization of the human VGSC b1B subunit The human b1B subunit is a novel splicing variant of the b1 subunit via alternative intron retention The retained intron encodes a novel extracellular, ... peptide Functional expression of the human b1B subunit with NaV1.2 in Xenopus oocytes To explore the regulatory function of the human b1B subunit, we injected cRNA of human b1B, as well as cRNA of the ... 2003 Cloning and characterization of Na+ channel b1B (Eur J Biochem 270) 4763 reaction (RT-PCR) and RACE-PCR Marathon-ReadyTM human adrenal gland and fetal brain cDNA libraries were purchased...

Báo cáo Y học: Puriﬁcation, characterization and subunits identiﬁcation of the diol dehydratase of Lactobacillus collinoides pot

... of the L collinoides diol dehydratase The three genes pduCDE encoding the three subunits of the diol dehydratase were cloned in the pQE30 expression vector system The synthesis of the three subunits ... study, we report the puriﬁcation, enzymatic characterization and analysis of the composition of the diol dehydratase of L collinoides MATERIALS AND METHODS Bacteria and culture conditions The ... belong to the dehydratase complex Characterization of the diol dehydratase The molecular mass of the native dehydratase was estimated by the Sephacryl S300H gel ﬁltration using ﬁve standards of known...

Báo cáo khoa học: Characterization and expression analysis of the aspartic protease gene family of Cynara cardunculus L. docx

... pattern of expression of a gene [34–39] To evaluate the relevance of the leader intron in cardosin expression, we deleted it from the 5¢-ﬂanking region of the genes (Fig 8) The deletion of the cardosin ... region of the cardosin B gene (from ) 147 bp to + 238 bp) .The 529 bp of the promoter region of the cardosin A gene that is relevant for gene expression in Arabidopsis and the corresponding region of ... of cardosin A, B and D genomic clones with the respective cDNAs (Fig 2) revealed the presence of an intron in the 5¢-UTR of the genes The nucleotide sequences of the cDNA and genomic clones of...

Báo cáo khóa học: Isolation and characterization of the Xenopus HIVEP gene family ppt

... combinations of degenerate and speciﬁc primers and by rescreening the cDNA libraries The partial clones of XHIVEP2 and -3 covered 5.9 and 3.2 kb of the respective 3¢ ends and included 3¢-UTRs and poly(A)-tails ... analyze the function of XHIVEP in the context of Xenopus embryogenesis (data not shown) However, the interpretation of the in vivo role of XHIVEP was not conclusive as the activator and repressor ... constructs by the generation of deletion mutants containing discrete functional domains of XHIVEP1 Thus, the cloning and characterization of the XHIVEP interacting factors would be of interest and should...

Báo cáo khoa học: Molecular cloning and functional expression of a gene encoding an antiarrhythmia peptide derived from the scorpion toxin pptx

... E.P., Martin-Eauclaire, M.F., Mansuelle, P & Sampieri, F (1991) An anti-insect toxin puriﬁed from the scorpion Androctonus australis Hector also acts on the alpha- and beta-sites of the mammalian ... Sampieri, F & Granier, C (1991) An anti-insect toxin puriﬁed from the scorpion Androctonus australis Hector also acts on the a- and b-sites of the mammalian sodium channel: sequence and circular dichroism ... obtained was displayed an ORF of 258 bp encoding a polypeptide of 85 amino acids and termed BmKIM The 5¢ and 3¢ UTRs of BmKIM cDNA are 17 bp and 76 bp, respectively A single AATAAA polyadenylation...

Báo cáo hóa học: " Molecular characterization and complete genome sequence of avian paramyxovirus type 4 prototype strain duck/Hong Kong/D3/75" potx

... 11.2 25 24. 7 24. 3 25.5 24. 5 22.3 25.8 22.7 24. 2 25.5 34. 3 24. 2 7.1 11.2 8.1 12 .4 14. 2 17.5 12 .4 15.1 14. 3 16.2 14. 3 16.8 22.2 14. 6 24 22.9 25 24. 9 20.8 26.5 21.2 23.3 23.5 23.5 23.2 11 .4 18.9 18.2 ... subunit, and 199, the most upstream cysteine in the F1 Deduced protein Size (aa) 34 14 37 42 - Mol wt (kDa) 45 7 393 2 24 137 369 566 44 6 120 569 2211 50.03 42 .02 23.98 14. 29 41 .45 61.32 47 .93 13 .41 ... ORF (nt) 3'UTR (nt) 1551 13 64 1365 1366 1293 1891 19 14 68 34 60 46 46 46 77 74 69 95 13 74 1182 675 41 4 1110 1701 1710 6636 117 136 644 906 106 116 42 - editing site, and none had a two G insertion...

Báo cáo hóa học: " Synthesis, Characterization, and Microwave Absorption Property of the SnO2 Nanowire/Parafﬁn Composites" doc

... where lr and er are the relative permeability and permittivity of the composite medium, c the velocity of EM waves in free space, f the frequency of the microwave, and d the thickness of the absorber ... working at 0.1–18 GHz Results and Discussion Figure shows the SEM and TEM images of the as-synthesized SnO2 NWs The diameters of the SnO2 NWs are about 100 nm, and the lengths are up to micron ... is either of the real part and imaginary part of the complex permittivity e and complex permeability l Ue, Ui, Um correspond to the parameter of the effective medium, the insulator, and the semiconductor...

Báo cáo y học: " Patterns of expansion and expression divergence in the plant polygalacturonase gene family" ppsx

... Figure The phylogeny of Arabidopsis and rice PGs The phylogeny of Arabidopsis and rice PGs The amino acid sequences for the glycosyl hydrolase 28 family motif were aligned The phylogeny was generated ... few of them were not retained 35x 5a The size of the plant PG family and the patterns of PG duplication in Arabidopsis indicate that the PG family expanded in both Arabidopsis and rice after their ... fruits [5-7] In addition, these enzymes are hypothesized to be involved in general housekeeping functions in plants [8] Among these hydrolytic enzymes, the PGs belong to one of the largest hydrolase...

Molecular characterization and developmental analysis of interferon regulatory factor 6 (IRF6) gene in zebrafish

... mouse, Fugu, and zebrafish 63 -64 3.5 Assembled sequences of irf6 genomic fragments 64 -66 3 .6 The irf6 gene locus on the LG22 in T51 RH panel and in the Ensembl zebrafish version (ZV6) 68 -69 3.7 Whole-mount ... morpholinos 85 3.14 Loss of irf6 function phenotypes 86- 87 3.15 Comparison of expression of molecular markers in irf6 morphants and wildtype 91-92 3. 16 Hematoxylin and eosin staining of intestines ... MOLECULAR CHARACTERIZATION AND DEVELOPMENTAL ANALYSIS OF INTERFERON REGULATORY FACTOR (IRF6) GENE IN ZEBRAFISH BEN JIN (M.Sc., National University of Singapore) A THESIS SUBMITTED...

Molecular characterization of the zebrafish ff1b gene

... promoter 176 Generation of the ff1bEx2EGFP transgenic zebrafish: a major step for the lineage tracing of ff1b- expressing cells 177 6.2.1 The EGFP transgene expression parallels the endogenous ... pattern of ff1b in the VMH 178 6.2.2 The EGFP transgene expression parallels the endogenous expression pattern of ff1b in the interrenal gland 180 6.2.3 The EGFP fluorescence unravels the axonal ... region of zebrafish cyp11a1 in comparison to the equivalent kb region in the gene promoter of Tetraodon, human, and mouse Overexpression of ff1b potentiates the transcriptional activity of zebrafish...

Molecular characterization and developmental analysis of the TGF beta 3 gene in zebrafish

... mutants, trunk notochords are present. This shows that spt mutation can suppress the 35 Molecular characterization and developmental analysis of the TGF Beta 3 gene in zebrafish flh mutation, suggesting that in the midline, flh is involved in promoting ... Molecular characterization and developmental analysis of the TGF Beta 3 gene in zebrafish phosphorylating TβRI on the serine and threonine residues and thus results in the activation of TβRI. In addition, the TβRII kinase domain can also phosphorylate itself. ... the molecule to the membrane of the early endosomes. The efficient recruitment of the 9 Molecular characterization and developmental analysis of the TGF Beta 3 gene in zebrafish Smad 2 and Smad 3 to the activated receptors for phosphorylation is facilitated by these ...

Investigating the transcriptional regualtion of the stevor multi gene family in plasmodium falciparum

... There are several classes of drugs used for the treatment of malaria: 4aminoquinolines, arylaminoalcohols, 8-aminoquinolines, artemisinines, antifolates and inhibitors of the respiratory chain ... modifications in the protocol future experiments will allow the elucidation of the lengths of these 5‟UTRs Repeating these transfections and following the suggested modifications in the protocol of the ... Summary The multi- gene family stevor in Plasmodium falciparum is thought to play a role in antigenic variation and virulence Although transcriptional regulation in the related var multi- gene family...

Molecular characterization and developmental expression patterns of the zebrafish twist gene family

... pattern with other species 83 4.5.1 Zebrafish twist1 a and twist1 b genes 83 4.5.2 Zebrafish twist2 85 4.5.3 Zebrafish twist3 86 4.6 Shared and unique expression sites of the zebrafish twist genes 86 ... proteins generated by the neighbor-joining method Figure 3.6: Gene structure of twist1 a, twist1 b, twist2 and twist3 Figure 3.7: RT-PCR of zebrafish twist genes Figure 3.8: Expression of zebrafish twist ... medaka, and human twist genes showed that the zebrafish twist1 a and twist1 b are coparalogs and co-orthologs of human TWIST1 Furthermore, zebrafish twist1 a and twist1 b are orthologous to medaka twist1 a...

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