cloning of recql4 a member of the recq helicase gene family
Báo cáo Y học: Cloning of the manganese lipoxygenase gene reveals homology with the lipoxygenase gene family doc
... 5¢-CCGTTCAGCGTCGAGA GCAAGG-3¢) and an antisense primer from the other sequence (MnS1, 5¢-TCTCGGGGATCGTGTGGAAGA GCA-3¢) amplified a fragment of 337 bp The latter contained WLLAK and the amino-acid ... (Pfam LOX; Fig 3) Plant and mammalian LOX contain a small N-terminal b-barrel domain and a large C-terminal and catalytic domain, as revealed by three-dimensional analysis [11,15] The function of ... Translation start pointa acaaaA73TGCGC… Met1ArgSerArgIle… 5¢-Donor …AGCg445tatgtgc …IleThrSer124 Translation end …GAGCGTC2058taaagg …PheLeuSerVal618 Branch signal t562gctaac 3¢-Acceptor ggctatag577CGT…...
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Báo cáo khoa học: Characterization and expression analysis of the aspartic protease gene family of Cynara cardunculus L. docx
... 764AF ) 234AF PromAR AAAAAGCAGGCTTGCTGTTCTAAGTGTACTAGCTGGAb AAAAAGCAGGCTCAAATTAAATCGACGGTTGAGb AAAAAGCAGGCTCAATGTAGTACCAATTGGGGTACCb AAAAAGCAGGCTGAGAAATCTATGGAATAAATAAAAATTAGGGb AGAAAGCTGGGTCGATGTTTCACTGAAACATTAATAGATATTCb,c ... AGAAAGCTGGGTCGATGTTTCACTGCAACCATGACb,c AAAAAGCAGGCTAACCACTATGATACCACTCACACCAb AGAAAGCTGGGTCGATGTTTCACTGCAACCATGACb,c AAAAAGCAGGCTCTTAACGACTGCGTATATCCTC PromBR PromBRDi AGAAAGCTGGGTCGATGTTTCACTGCAACCATGACb,c AGAAAGCTGGGTCGATGTTTCACTCTTATTTGATGGAAGCAATGAAGGb,c,d ... GATCTCGGCTGGGAAAGCG CardBR ATACCATTGCAGTCTACTATC FLCBR ActF TTTATTGGACCATTTTATTCCGG GATATGGAAAAGATCTGGCATCAC ActR TCATACTCGGCCTTGGAGATCC ) 2912AF AAAAAGCAGGCTATGAATTGCTAGAGTTGGTTAATGCb ) 1792AF ) 1263AF...
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Báo cáo khóa học: Isolation and characterization of the Xenopus HIVEP gene family ppt
... Sites of mutation are underlined: wt, 5¢-AGAGAGAA TGAGAGGCTTCCCAATAGC-3¢; mut1, 5¢-AGAGAG AATGATAGGCTTCACAATAGC-3¢; mut2, 5¢-AGAG AGAATGATAGGCTTCCCAATAGC-3¢; mut3, 5¢-AGA GAGAATGAGAGGCTTCACAATAGC-3¢ ... 971–980) and a XHIVEP1 gene specific primer set derived from the additional sequence obtained in the first amplification (5¢-GARAAYTTYGARAAYCAYAARAARTTYTAYTG-3¢ and 5¢-AGTTCTAATGCTATGTTTGGATGC-3¢) afforded ... (Molecular Dynamics) Primers and conditions used for RT-PCR were as follows: XHIVEP1, 5¢-ATCCAGAGGCAGAAGCAG-3¢ and 5¢-CTGCATT CAGAGTAAGCC-3¢, 60 °C, 29 cycles; XHIVEP2, 5¢-AAGCAGAGGAATGCAGTAG-3¢ and...
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Investigating the transcriptional regualtion of the stevor multi gene family in plasmodium falciparum
... tatgtaaaaatataaataattattacaataatttaaatatataattaaacaattattgcattttcagtaaa tattcattaatcgtctttttcttttttagGAGAATTATCTAAATAACCATTATAATGTATGTCTCATTCAA AACAAGACCAAAAGAACAACG B ATGAAGATGTATTACCTTAAAATGATATTGTTTAACTTTTTAATTAATACTTTATTATTACCACATTAT ... has been used previously to study the trafficking of STEVOR in the asexual stage (Przyborski et al 2005) A ATGAACATGTATTACGTTAAAATGTTATTGTTTGCCTTTTTAATAAATACATTAGTATTACCACATTATg tatgtaaaaatataaataattattacaataatttaaatatataattaaacaattattgcattttcagtaaa ... GGCAGATCTAATGCACTATTTAAGAAAACCTCTCAA – 3' 59.27 gPF10_0395_R 1_NI 5' – CCACCTAGGATAATGTGGTAATAATAAAGTATTAATTAAAAAGTTAAAC - 3' 58.62 FL 200 F 5‟ – GGCAGATCTAGCTATGAAACGATATGGGCT – 3‟ 56.46 FL 1200 R 5‟ – CCACCTAGGCATAATCATAGGACCTCTCACACAC...
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Molecular characterization and developmental expression patterns of the zebrafish twist gene family
... gctaaacttg actcgatgag tcaataaaca aaaaaaaaaa Figure 2.9: gtgttggtga tagctaacag aacaataaga aaaaaaaaaa tggtgtgaaa actaagagtt tgaaaaaaaa aattaaaaaa ataaactgaa atgaagcgta cagcaggaga tacgctgatt atcaagagct ... ggattataaa actacgacat aaacatgtgc ctgacagcaa aacgaatgaa atacttttat gggaaaattc gggagcagga ttccgtccac caaccagcat ggaaatcggc ttatttattg tggagcgtca attacagtca aaaacagaga ggcgtcatat tcatatcagt atgattgtca ... atcaagagct tgaaaattca aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa cDNA sequence of zebrafish twist3 Positions of primers are indicated by arrows and the 3’UTR region used for the synthesis of RNA...
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Báo cáo khoa học: Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis Functional analysis of the promoter identifies a calcium sensitive region required for basal activity doc
... TTAGG-3Â) and the 3Â specic oligonucleotides (5Â-GA AGATCTACCACACCTCCTCATCTCC-3Â) for amplication of the region from )180 to )36 and (5Â-GAAGAT CTAACTAGATTTTACCATTGG-3Â) for amplication of the ... acag|gtaag tatg|gtaag tatg|gtaag agag|gtaag g(t)5aacag|aagaa c(t)4gtatacag|actc a( t)4cag|atcc c(t)4ag|aatc Not in coding region I I II (2929) (986) (1985) (1490) Table Comparison between exon ... (5Â-CACGCAAGCTTCT CTTGAGTCTCTATGAAGG-3Â) and the 5Â specic oligonucleotides (5Â-CCGGAGCTCGAGACTCTTAGTAA ATGTGCCCC-3Â) for amplication of the fragment from )949 to +33 (5Â-CCGGAGCTCGAGCCGCTAAAGA...
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Báo cáo Y học: Characterization of a Saccharomyces cerevisiae NADP(H)-dependent alcohol dehydrogenase (ADHVII), a member of the cinnamyl alcohol dehydrogenase family pptx
... the genomic DNA from the S cerevisiae S288C strain using the oligonucleotides 5¢GGCGAGCTCAAAATGCTTTACCCAGAAAAATT TGAGG-3¢ and 5¢GGCTCTAGACTATTTATGGAA TTTCTTATC-3¢ that introduced SacI and XbaI ... although the kcat and Km values with ADHVII are approximately half the values found for ADHVI The catalytic efficiencies towards the oxidation of cinnamyl alcohol and several aliphatic alcohols are ... activity Cinnamaldehyde Phenylacetaldehyde Benzaldehyde Coniferaldehyde Veratraldehyde Anisaldehyde Vanillin Propanal Butanal Pentanal Hexanal Heptanal Octanal 2-Methylpropanal 2-Methylbutanal 3-Methylbutanal...
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Báo cáo sinh học: " Open Access Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells" docx
... TGTTTCGGCGACAGGCAGACAGACGACAGGCAGACGTAAAAGACAGCCGGTCCGTCCGTCGCTCGCCTTAGAGATGTGGGCCTCTGGGCGCGGGTGGGGTTCCGGGCTTGACCGCGCGGCCGAGCCGG Consensus (1851) TGTTTCGGCGACAGGCAGACAGACGACAGGCAGACGTAAAAGACAGCCGGTCCGTCCGTCGCTCGCCTTAGAGATGTGGGCCTCTGGGCGCGGGTGGGGTTCCGGGCTTGACCGCGCGGCCGAGCCGG ... BbsI CG ACCTCCG TTG G G G AG AAG G G G AG TCTTCTCG TAG ACCG AG AAG ACCTACCTG CAACAAAAAATG G T G G G CTG AG CTTCAACCCCCCCTCTCAG AG AAG CTCATCTTCTG CTG ATG ACCG TTTTTTACA G G G TTG 80 CAT (ng/well) ... GCATGTGCGGGCAGGAAGGTAGGGGAAGAC GATC Drosophila TATTGTACCTGGAGATATATGCTGACACGC 726,713 553, 500 427, 417, 413 311 249 200 GCGGGTTCAAAAACTACTATAGGTAGGCAG Human TTTTTTGTTGCCAGGTAGGTGCTGACACGT MDCK...
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Báo cáo hóa học: " Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells" pdf
... TGTTTCGGCGACAGGCAGACAGACGACAGGCAGACGTAAAAGACAGCCGGTCCGTCCGTCGCTCGCCTTAGAGATGTGGGCCTCTGGGCGCGGGTGGGGTTCCGGGCTTGACCGCGCGGCCGAGCCGG Consensus (1851) TGTTTCGGCGACAGGCAGACAGACGACAGGCAGACGTAAAAGACAGCCGGTCCGTCCGTCGCTCGCCTTAGAGATGTGGGCCTCTGGGCGCGGGTGGGGTTCCGGGCTTGACCGCGCGGCCGAGCCGG ... BbsI CG ACCTCCG TTG G G G AG AAG G G G AG TCTTCTCG TAG ACCG AG AAG ACCTACCTG CAACAAAAAATG G T G G G CTG AG CTTCAACCCCCCCTCTCAG AG AAG CTCATCTTCTG CTG ATG ACCG TTTTTTACA G G G TTG 80 CAT (ng/well) ... GCATGTGCGGGCAGGAAGGTAGGGGAAGAC GATC Drosophila TATTGTACCTGGAGATATATGCTGACACGC 726,713 553, 500 427, 417, 413 311 249 200 GCGGGTTCAAAAACTACTATAGGTAGGCAG Human TTTTTTGTTGCCAGGTAGGTGCTGACACGT MDCK...
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Báo cáo khoa học: Novel repressor of the human FMR1 gene ) identification ¨ of p56 human (GCC)n-binding protein as a Kruppel-like transcription factor ZF5 ppt
... siRNA selection [43] The first siRNAZF5 5¢-GGUUGAGGAUGUGAAAUUCUU-3¢ and 5¢-GAAUUUCACAUCCUCAACCUU-3¢) matched bases 192–210; the second siRNAZF5 (5¢-GAGGAAGCAUGA GAAACUCUU-3¢ and 5¢-GAGUUUCUCAUGCUUCCU ... Federation The first pair of primers (5¢-GGCCTTCAAGGCATTAAG-3¢, 5¢-AAACAAATGG CCTGTCCG-3¢) spanned a 958 bp 5¢-part of the ZF5 coding region; the second pair (5¢-CCCCTCAAGCCTT AACAT-3¢, 5¢-TCTCCACTTTCCAGGCAA-3¢) ... USA), Amersham Biosciences (Piscataway, NJ, USA), Roche Applied Science (Mannheim, Germany), Invitrogen (Carlsbad, CA, USA), Promega (San Luis Obispo, CA, USA) and from local Russian manufacturers...
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Báo cáo Y học: Cloning, chromosomal localization and characterization of the murine mucin gene orthologous to human MUC4 pdf
... TCTACgtgagtccgg ACCAGgtgagtcatt atgtgctcagTTCAC cctttcctagGAATA ATCAGgtgagccttt TTGGGgtgagtggat cctttcctagGAATA caacttccagACCAA TCGGGgtgagtagac TCCAGgtaagatcgg ccacccccagAGCAA ttgcctgcagTGGAG ... tttcaagaagATGCT CTCAGgtgagtcagc ttcactccagGAACC ccatgtccagGATTG ATCAGgtagctgcca TCAGGgtaagtgata acgtgtccagGAATG ccttgtctagGCATT GAGAGgtgaggccat TCTACgtgagtctct cctggcccagGAGTT ttgtgctcagGTTAC ... ccctttacagCTCTG cactctgcagCTCTG TACGGgtatggctaa CCTAGgtaccgccag ttatctccagAGCTT ccatctccagAACTT CCTCGgtcagtgctg CCTCGgtcagtgctg tccattgtagGTGGC tctaacctagGTGGC CGAACgtaagtagag CGAATgtaagtggga ttccatacagCTCTC...
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Báo cáo Y học: Propionate CoA-transferase from Clostridium propionicum Cloning of the gene and identi®cation of glutamate 324 at the active site pdf
... propionate CoAtransferase based on these data was glutamate 324 (Fig 3) Detection of glutamate 324 as the catalytic carboxylate of propionate CoA-transferase As the sequence analysis did not allow an unequivocal ... protein database search programs Nucleic Acids Res 25, 3389±3402 26 Takami, H., Nakasone, K., Takaki, Y., Maeno, G., Sasaki, R., Masui, N., Fuji, F., Hirama, C., Nakamura, Y., Ogasawara, N., Kuhara, ... mM) at 25 °C Note that the activity in the cell free extract is the sum of CoA-transferase and phosphotransacetylase and that the latter enzyme is completely separated from the CoA-transferase...
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Báo cáo Y học: Nuclear proteins that bind to metal response element a (MREa) in the Wilson disease gene promoter are Ku autoantigens and the Ku-80 subunit is necessary for basal transcription of the WD gene ppt
... Taq (Takara, Japan) in a total volume of 50 lL containing 55 pg of competitor At this concentration, the band intensity of the amplified product from the competitor was same as that of the product ... proteins that bind specifically to MREa play an important role as a basal regulator of WD gene transcription MATERIALS AND METHODS Cell culture The human hepatoma cell line HepG2 was obtained from ... normalized to b-gal activity, and the relative increases were calculated as the ratio of normalized activity in MREa/mutant transfected cells to that in pGLpro transfected cells Data represent the...
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Báo cáo khoa học: Molecular cloning of the ecdysone receptor and the retinoid X receptor from the scorpion Liocheles australasiae pot
... TCYTCYTGNACNGCYTC CAYTTYTGRTANCKRCARTA GCAAGCTGGAAAAGAGTAATGTGAC ATACTGGCAGCGATTCCTTTGAC AGCCTTTACAACCTTCACAGC ATAGCTGTGAAGGTTGTAAAGG GACAAACGTCAAAGGAATCG a N means a mixture of A, T, G and C In the ... AGACTCCCGTTTGATGGCACACTG GCATTCCGACACTGAGGCACTTTT GAAAAAGTGCCTCAGTGTCGGAATG CAGTGTGCCATCAAACGGGAGTCTA F1 F2 F3 R1 R2 R3 RR1 RR2 RF1 RF2 ATHTGYGGNGAYMGNGC GGNAARCAYTAYGGNGTNTA GATTCAGATCCCGACCATAAAGA TCYTCYTGNACNGCYTC ... L australasiae EcR -A O moubata EcR -A A americanum EcR -A1 B germanica EcR -A L migratoria EcR -A T castaneaum EcR -A L decemlineata EcR -A T molitor EcR -A A mellifera EcR -A A aegypti EcR -A D meelanogaster...
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Báo cáo khoa học: Myocyte enhancer factor 2 (MEF2) is a key modulator of the expression of the prothoracicotropic hormone gene in the silkworm, Bombyx mori ppt
... lobes and at the midline in the subesophageal ganglion (SG) (Fig 1A) The axons emanating from the somata of the two pairs of lateral cells extend towards the pars intercerebral with some arborization ... black and white images An area adjacent to the area of interest (A) and an area from an image lacking a specimen (N) were scanned as the background signals When PTPCs were not visible, the focal ... development of multicellular organisms [15] There are four isoforms (A D) of mammalian MEF2, and they have high homology within the 56-amino-acid MADS box at their N-termini and within an adjacent 29-amino-acid...
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Báo cáo khoa học: "Molecular cloning of the cDNA of canine homeodomaininteracting protein kinase 2" ppsx
... that the functions of canine HIPK2 were similar Molecular cloning of the cDNA of canine homeodomain-interacting protein kinase to those of human and murine HIPK2, because the deduced amino acid ... was similar to those of human and mouse The deduced amino acid sequence of canine HIPK2 was also very similar to those of human and murine HIPK2 The spinal cord was selected because HIPK2 mRNA ... sequences of canine HIPK2 contained all these domains For instance, HIPK2 acts as a transcriptional corepressor for homeoproteins and localizes to nuclear speckles In the N-terminal of the catalytic...
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Báo cáo khoa học: " Sequence analysis of the S1 glycoprotein gene of infectious bronchitis viruses: identification of a novel phylogenetic group in Korea" docx
... (5'TG AAAACTGAACAAAAGAC3') together with one of the reverse primers S1OLIGO3' (CTAAACTAACATAAGGG C3') or degenerate3'-2 (5'CCATAAGTAACATAAGGG CAA3') [14,16] The RT reaction for synthesis of cDNA ... isolates using the QIAamp MinElute Virus Spin Kit (QIAGEN, USA) Amplification of the S1 gene by RT-PCR was performed using one of the forward primers S1OLIGO5' (5'TGA AAACTGAACAAAAGA3') or NEWS1OLIGO5' ... - Arkansas KM91‡ KM91 KM91 KM91 KM91 Variant KM91 Variant Variant Variant Variant GenBank accession numbers AY790368 AY790359 AY790360 AY790358 AY790362 AY790361 AY790366 AY790365 AY790363 AY790364...
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Báo cáo y học: " A promoter haplotype of the interleukin-18 gene is associated with juvenile idiopathic arthritis in the Japanese population" pps
... allele2 FAM-CACTTATCTGTAGAGCTT-MGB SNP4F CTGGCAATTCTGCCTTGTTTCAG SNP4 allele1VIC- CCGAAGATAAAAGAATC-MGB SNP4R GGATTACAGCCGTGAGCCA SNP4 allele2 FAM- CGAAGATAGAAGAATC-MGB SNP5F AAGCTGAGGCAGGAAGATCAC ... ACAGGTTTTGGAAGGCACAGA SNP11 VIC- ACGGAAGAAAAGATTT-MGB SNP11R AATAAAGTGGCAGAGGATACGAGTACT SNP11 FAM-ACGGAAGAAAACATTT-MGB SNP12F AATTGTCTCCCAGTGCATTTTGC SNP12 allele1 VIC-CTGCCAACTCTGGCTG-MGB SNP12R TCTTCCCGAAGCTGTGTAGACT ... FAM-TGAAGACCCCGGGC-MGB SNP9F GAAAGTTTTAACACTGGAAACTGCAA SNP9 allele1 VIC-TTTTGGTAGCCCTCTC-MGB SNP9R TTACACTTTCTGCAACAGAAAGTAAGC SNP9 allele2 FAM-TTTTGGTATCCCTCTCC-MGB SNP11F ACAGGTTTTGGAAGGCACAGA...
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Báo cáo y học: " A promoter SNP rs4073TA in the common allele of the interleukin 8 gene is associated with the development of idiopathic pulmonary fibrosis via the IL-8 protein enhancing mode" docx
... the statistical analysis The inter- and intraassay coefficients of variance were below 10% Protein concentration of BAL samples was measured for standardization using a micro BCA protein assay ... were managed and analyzed using SAS version 9.1 (SAS Inc., Cary, NC, USA) Statistical power of single associations was calculated with false-positive rate of 5% and four given MAFs and sample ... results may provide the clue of the genetic contribution to the pathogenesis of IPF Additional material Additional file 1: The fluorescence labeled allelic probe for amplification of IL8, IL8RA and...
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A study of the recombination activating gene 1 in the zebrafish nervous system
... (Kapitonov and Jurka, 2005) In addition, Transib transposons carry a pair of 38-bp terminal inverted repeats consisting of a conserved 5’-CACAATG heptamer and an AAAAAAATC-3’ nonamer separated by a variable ... ABBREVIATIONS A/ P Anterior/posterior abcb3 ATP-binding cassette, subfamily B member ACP Annealing Control Primer ANOVA Analysis of Variance AOB Accessory Olfactory Bulb AP Alkline Phosphatase ATM Ataxia ... biochemical characterization of RAG1 and has largely focused on the core region of RAG1 (384-1008 aa) and RAG2 (1-387 aa) While the contribution of RAG2 to the V(D)J recombination is not clear, many aspects...
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