Cell based screening assay for inhibitors of porcine circovirus type2 (PCV2) replication 2

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Ngày tải lên: 27/01/2014, 08:52

... Analysis of HA and quantitative real-time PCR data employed for the determination of JC viral load A – C: Analysis of HA and quantitative real-time PCR data employed for the determination of JC viral ... G, Fischer S, Major EO: Comparison of PCR- southern hybridization and quantitative real-time PCR for the detection of JC and BK viral nucleotide Page...

Ngày tải lên: 19/06/2014, 08:20

... Analysis of HA and quantitative real-time PCR data employed for the determination of JC viral load A – C: Analysis of HA and quantitative real-time PCR data employed for the determination of JC viral ... microtiter and transwell plates Semiquantitative polymerase chain reaction (PCR) and quantitative real-time PCR have been recently developed and employed fo...

Ngày tải lên: 20/06/2014, 04:20

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Ngày tải lên: 07/08/2014, 18:21

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Ngày tải lên: 07/08/2014, 18:21

... Figure obtained by Easy CD4 assay the CD4+ cell counts Bland Altman plot analysis of( Guava) andTflow cytometry Bland Altman plot analysis of the CD4+ T cell counts obtained by Easy CD4 assay (Guava) ... use of flow cytometry in the resource poor settings We compared the CD4+ T cell estimations in HIV infected individuals using EasyCD4 System with the conventional...

Ngày tải lên: 10/08/2014, 05:20

... the immobilization methodologies discussed could be applied for rapid identification, epidemiological studies, genetic evaluation, and forensic investigation Page of 10 (page number not for citation ... serve as potential carriers and/or anchor materials for biomolecules Magnetic nanoparticles for example have been reported as support structures for biological materials including...

Ngày tải lên: 11/08/2014, 00:22

... be a very timely addition to our evaluation techniques 16 Summary 17 Surface SEMG of swallowing is a simple and reliable method for screening and initial evaluation of dysphagia and odynophagia ... normative database and standard analysis Table 2: Quick reference simplified set of normative data for electric activity obtained by surface EMG for masseter...

Ngày tải lên: 11/08/2014, 20:20

... article as: Zhao et al.: Rapid detection of porcine circovirus type using a TaqMan-based real-time PCR Virology Journal 20 10 7:374 Submit your next manuscript to BioMed Central and take full advantage ... 4 .2 105 24 .49 0 .25 89 1.05 24 .03 0.5597 2. 3 103 30.98 0 .20 92 0.59 30 .22 0.56 32 1.9 SD, standard deviation; CV, Coefficient of variation using a Taq...

Ngày tải lên: 11/08/2014, 21:21

... 2006BAD0 6A0 3) Author Details Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiologic Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural ... early diagnosis of many other animal viral diseases [7,8] In this study a one- step real-time RT-PCR assay was developed and evaluated for detectio...

Ngày tải lên: 12/08/2014, 04:20

... unlikely to be the target of ATA Disruption of phosphorylation of ERK by ATA was an indication that ATA inhibited the PDGFR/ ERK pathway upstream of ERK Subsequent analysis indicated that phosphorylation ... inhibitory potency of ATA The positions and number of carboxyl and hydroxyl groups were essential for PDGFR inhibition, as indicated by the fact that no compounds in the...

Ngày tải lên: 14/08/2014, 08:20

... αglucosidase and α-amylase can significantly reduce the post-prandial increase in blood glucose after a meal, with starch as the major calorie intake The apparent advantage of the starch hydrolase inhibitors ... linear range of gelatinized starch Table Accuracy and precision of the high- throughput method Table IC50 and acarbose equivalence of inhibitors obtaine...

Ngày tải lên: 10/09/2015, 09:01

... to  optimize  these  cell based screening assays  for inhibitors of PCV2  replication.       Cell Based Screening Assay for Inhibitors of Porcine Circovirus Type 2 (PCV2) Replication       ... second  messenger assays, (2) reporter gene assays, and (3) cell proliferation assays. Second messenger  Cell Based Screening Assay for Inhibitors of Porcine Circovirus Type 2 (PCV2) Replica...

Ngày tải lên: 03/10/2015, 11:36

... required tweaking three parameters: 1) cell seeding density, 2)  MOI, and 3) duration of infection prior to  fixation.   Cell Based Screening Assay for Inhibitors of Porcine Circovirus Type 2 (PCV2) Replication       Page |  62        4. DISCUSSION    For uniformity of results, it was necessary to have an even  monolayer of cells per well and any  ... rates. U0 126  is a MEK 1  /2 inhibit...

Ngày tải lên: 13/10/2015, 16:41